We narrowed to 9,642 results for: SUB

1. pMAL c2x Tral N

   Plasmid

   #113009

   Purpose

   For bacterial expression of MBP fusion of N terminal region of Drosophila Tral

   Depositor

   Terry Orr-Weaver

   Article

   Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

   Insert

   N terminus of tral (tral Fly)
   

   Expression

   Bacterial

   Mutation

   amino acids 1-355

   Available Since

   Aug. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMAL c2x Tral FL-15A

   Plasmid

   #113011

   Purpose

   For bacterial expression of MBP fusion of full-length Drosophila Tral with phosphomutations (15A)

   Depositor

   Terry Orr-Weaver

   Article

   Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

   Insert

   full length tral (tral Fly)
   

   Expression

   Bacterial

   Mutation

   15 PNG phosphorylation sites mutated to A

   Available Since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMAL c2x Tral C

   Plasmid

   #113008

   Purpose

   For bacterial expression of MBP fusion of C terminal region of Drosophila Tral

   Depositor

   Terry Orr-Weaver

   Article

   Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

   Insert

   C terminus of tral (tral Fly)
   

   Expression

   Bacterial

   Mutation

   amino acids 356-652

   Available Since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMAL c2x Tral C 15A

   Plasmid

   #113007

   Purpose

   For bacterial expression of MBP fusion of C terminal region of Drosophila Tral phosphomutant (15A)

   Depositor

   Terry Orr-Weaver

   Article

   Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

   Insert

   C terminus of tral (tral Fly)
   

   Expression

   Bacterial

   Mutation

   PNG phosphorylation sites mutated to A

   Available Since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-AuroraB L154A/H250Y

   Plasmid

   #108491

   Purpose

   expression of EGFP-AuroraB Analog sensitive (L154A/H250Y)

   Depositor

   Susanne Lens

   Article

   Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

   Insert

   AuroraB (AURKB Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Analog sensitive L154A/H250Y

   Promoter

   CMV

   Available Since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lrrc4c-AP-His

   Plasmid

   #71959

   Purpose

   Expresses the extracellular region of the LRRC4C protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Lrrc4c (Lrrc4c Mouse)
   

   Tags

   AP-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-CHRFAM7ADelta2bp-YFP

   Plasmid

   #62637

   Purpose

   mammalian expression of human duplicated Alpha7-2 bp deletion (CHRFAM7A-∆2bp) with YFP fused into M3-M4 loop

   Depositor

   Henry Lester

   Article

   Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

   Insert

   CHRFAM7A (CHRFAM7A Human)
   

   Expression

   Mammalian

   Mutation

   there is a 2-bp deletion in exon 6 of CHRFAM7A an…

   Promoter

   CMV

   Available Since

   March 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. Bidirectional MYC 5'UTR Dual Fluorescence Reporter

   Plasmid

   #229498

   Purpose

   This is a lentiviral reporter for translation initiation under the control of the Myc 5'UTR, which produces destabilized GFP (dsGFP). There is a control destabilized mCherry (dsmCherry) as a control.

   Depositor

   Davide Ruggero

   Article

   Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/

   Insert

   MYC 5'Untranslated region + destabilized GFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Feb. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAVS1-tet-iCas9-BFP2

   Plasmid

   #125519

   Purpose

   all-in-one-inducible Cas9

   Depositor

   Jason Moffat

   Article

   Mair et al Cell Rep. 2019 Apr 9;27(2):599-615.e12.

   Insert

   AAVS1-TET-ON 3G-SpCas9-P2A-BFP2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET23a-H6-TEV-cAbl

    Plasmid

    #214234

    Purpose

    Bacterial expression of the kinase domain of cAbl with a TEV-cleavable N-terminal 6xHis affinity tag; For in vitro tyrosine phosphorylation in peptides/proteins and for kinase specificity screenings

    Depositor

    Neel Shah

    Article

    Shah et al Elife. 2018 Mar 16;7. pii: 35190. doi: 1

    Insert

    ABL1 Kinase domain (ABL1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223_SMURF2_WT

    Plasmid

    #82259

    Purpose

    Gateway Donor vector containing SMURF2 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SMURF2 (SMURF2 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJet-CMV-IARS1-GFP

    Plasmid

    #212322

    Purpose

    EGFP-tagged IARS1

    Depositor

    Sabine Fuchs

    Article

    Kok et al Nucleic Acids Res. 2025 Jan 7;53(1):gkae

    Insert

    IARS (IARS1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Wildtype

    Promoter

    CMV

    Available Since

    Nov. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. TRIPZ-bioGEF-UBwt-PURO

    Plasmid

    #208045

    Purpose

    Enables inducible expression of low-affinity Avitag-Ubwt; to perform bioE3; selection with puromycin

    Depositor

    Rosa Barrio, James Sutherland

    Article

    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

    Insert

    Ub (UBC Human)
    

    Use

    Lentiviral

    Tags

    Low-affinity Avitag

    Promoter

    tetO/UBC

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lenti-EFS-BirAopt-GSQ-RBXN-P2A-BLAST

    Plasmid

    #208048

    Purpose

    Enables constitutive expression of BirAopt alone; RBXN represents a EcoR1-BsiW1-Xba1-Not1 polylinker; to perform bioE3; selection with blasticidin

    Depositor

    Rosa Barrio, James Sutherland

    Article

    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

    Insert

    BirAopt
    

    Use

    Lentiviral

    Mutation

    BirAopt is human-codon-optimized BirA (PMID 15707…

    Promoter

    EFS

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. C1QBP-bio-His

    Plasmid

    #53330

    Purpose

    Expresses full-length Complement component 1 Q subcomponent-binding protein ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    C1QBP (C1QBP Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pWZL hygro Flag HA TRAP220 wt

    Plasmid

    #17433

    Purpose

    Retroviral expression of Med1/TRAP220 in mammalian cells

    Depositor

    Kai Ge

    Article

    Ge et al Mol Cell Biol. 2008 Feb . 28(3):1081-91.

    Insert

    TRAP220 (MED1 Human)
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Available Since

    Feb. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMega-MaPylRS

    Plasmid

    #200225

    Purpose

    The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with Nε-Boc-lysine.

    Depositor

    Alanna Schepartz

    Article

    Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155

    Inserts

    Pyrrolysyl-tRNA synthetase
    pyrrolysyl-tRNA
    

    Tags

    none

    Expression

    Bacterial

    Mutation

    none

    Promoter

    proK-lacO and tacI

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR223_WNT5A_WT

    Plasmid

    #82240

    Purpose

    Gateway Donor vector containing WNT5A , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    WNT5A (WNT5A Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Cry2 mCherry GRK2 ct in pcDNA3.1

    Plasmid

    #64212

    Purpose

    mCherry fused between Cry2 and GRK2ct to study cell migration

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

    Inserts

    Cry2 PHR (CRY2 Mustard Weed)
    GRK2 ct (GRK2 Human)
    

    Tags

    mcherry

    Expression

    Mammalian

    Promoter

    CMV and cmv

    Available Since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pKGmDUET-NgBR/hCIT

    Plasmid

    #203173

    Purpose

    Expression of human cis-prenyltransferase complex for purification

    Depositor

    William Sessa

    Article

    Grabinska et al J Biol Chem. 2017 Oct 20;292(42):17351-1

    Inserts

    NUS1 (NUS1 Human)
    DHDDS (DHDDS Human)
    

    Tags

    Strep-Tag II-TEV and internally 6HIS tagged

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGM234

    Plasmid

    #220177

    Purpose

    Expresses exogenous wild-type NAC complex members (NACA and BTF3)

    Depositor

    Jonathan Weissman

    Article

    Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

    Insert

    NACA and BTF3 (NACA, BTF3 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG (NACA); 6xHis (BTF3)

    Available Since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. R702-X80-635: His6-tev-Hs.NF1opt (1-2818) isoform 2

    Plasmid

    #159575

    Purpose

    Baculovirus protein expression of His6-tev-Hs.NF1opt (1-2818) isoform 2

    Depositor

    Dominic Esposito

    Article

    Sherekar et al J Biol Chem. 2020 Jan 24;295(4):1105-111

    Insert

    His6-tev-Hs.NF1opt (1-2818) isoform 2 (NF1 Human)
    

    Tags

    His6-tev

    Expression

    Insect

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDONR223_PKIA_WT

    Plasmid

    #82153

    Purpose

    Gateway Donor vector containing PKIA , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    PKIA (PKIA Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA 3.2/V5-DEST CDC42EP1 WT

    Plasmid

    #69819

    Purpose

    Expresses CDC42EP1-V5 in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    CDC42 effector protein (CDC42EP1 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Anti-TrpC4 [N77/15.1R]

    Plasmid

    #114504

    Purpose

    Mammalian Expression Plasmid of anti-TrpC4 (Rat). Derived from hybridoma N77/15.1.

    Depositor

    James Trimmer

    Article

    Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-TrpC4 (Rattus norvegicus) recombinant mouse monoclonal antibody (Trpc4 Mouse)
    

    Expression

    Mammalian

    Promoter

    dual CMV

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. R702-X38-635: His6-Hs.NF1opt (1-2839) isoform 1

    Plasmid

    #159576

    Purpose

    Baculovirus protein expression of His6-Hs.NF1opt (1-2839) isoform 1

    Depositor

    Dominic Esposito

    Article

    Sherekar et al J Biol Chem. 2020 Jan 24;295(4):1105-111

    Insert

    His6-Hs.NF1opt (1-2839) isoform 1 (NF1 Human)
    

    Tags

    His6

    Expression

    Insect

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLV-SspB-HaloTag-p63DH

    Plasmid

    #176129

    Purpose

    Heterodimerization with iLID, RhoA activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    RHOGEF p63 (ARHGEF25 Human)
    

    Use

    Lentiviral

    Available Since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTT3 Flrt2-myc

    Plasmid

    #72192

    Purpose

    Express full-length Flrt2with a C-terminal myc tag

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Flrt2 (Flrt2 Mouse)
    

    Tags

    myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Anti-WAVE1/SCAR [K91/36R]

    Plasmid

    #128629

    Purpose

    Mammalian Expression Plasmid of anti-WAVE1/SCAR (Human). Derived from hybridoma K91/36.

    Depositor

    James Trimmer

    Article

    Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-WAVE1/SCAR (Human) recombinant mouse monoclonal antibody (WASF1 Mouse)
    

    Expression

    Mammalian

    Promoter

    Dual CMV

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. ITSN-mcherry-sspB

    Plasmid

    #85220

    Purpose

    Role of Cdc-42 selective GEF domain from ITSN in immune cell migration

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50.

    Inserts

    ITSN1(1225-1575) (ITSN1 Human)
    mcherry
    sspB
    

    Tags

    NA

    Expression

    Mammalian

    Mutation

    R73Q

    Promoter

    CMV

    Available Since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDONR223_RHEB_WT

    Plasmid

    #81257

    Purpose

    Gateway Donor vector containing RHEB , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    RHEB (RHEB Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Tet-O-FUW-TRPS1-P2A-mCherry

    Plasmid

    #203851

    Purpose

    Tet-inducible lentiviral plasmid expressing TRPS1-P2A-mCherry, with ORF specific barcode close to 3'LTR site

    Depositor

    Andrea Califano

    Article

    Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

    Insert

    TRPS1 (TRPS1 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET15-ZmPYK

    Plasmid

    #220230

    Purpose

    Inducible expression of Zymomonas mobilis pyruvate kinase

    Depositor

    Liskin Swint-Kruse, Aron Fenton

    Article

    Swint-Kruse et al Database (Oxford). 2023 May 3;2023:baad0

    Insert

    Zymomonas mobilis pyruvate kinase (pyk, ZMO0152 Zymomonas mobilis)
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pEcRNAP6

    Plasmid

    #128940

    Purpose

    pET21a derivative encoding E. coli core RNA polymerase subunits: RpoA, B, C-ppx(His)10, Z

    Depositor

    Seth Darst

    Article

    Twist et al Protein Sci. 2011 Jun;20(6):986-95. doi:

    Inserts

    RpoA (rpoA E. coli)
    RpoB
    RpoC-ppx(His)10
    RpoZ
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    PreScission protease site (GE Healthcare; LEVLFQG…

    Promoter

    T7

    Available Since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Anti-VGlut2 [N29/29R]

    Plasmid

    #114501

    Purpose

    Mammalian Expression Plasmid of anti-VGlut2 (Rat). Derived from hybridoma N29/29.

    Depositor

    James Trimmer

    Article

    Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-VGlut2 (Rattus norvegicus) recombinant mouse monoclonal antibody (Slc17a6 Mouse)
    

    Expression

    Mammalian

    Promoter

    dual CMV

    Available Since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBa-KIF5C 559-tdTomato-FKBP

    Plasmid

    #64211

    Purpose

    KIF5C motor protein aa1-559 with C-terminal tandem dimer Tomato and FKBP tags

    Depositor

    Marvin Bentley, Gary Banker

    Article

    Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

    Insert

    Kif5c (Kif5c Rat)
    

    Tags

    FKBP and tdTomato

    Expression

    Mammalian

    Mutation

    motor domain aa1-559

    Promoter

    chicken Beta Actin

    Available Since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. RCAS-Flag-BRAFV600E

    Plasmid

    #126615

    Purpose

    Retroviral expression of Flag tagged human oncogenic BRAFV600E

    Depositor

    Yi Li

    Article

    Bu et al Cancer Res. 2019 Jan 1;79(1):61-71. doi:

    Insert

    BRAF (BRAF Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    V600E

    Available Since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMega-MaFRSA

    Plasmid

    #200226

    Purpose

    The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with meta-trifluoromethylphenylalanine..

    Depositor

    Alanna Schepartz

    Article

    Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155

    Inserts

    Pyrrolysyl-tRNA synthetase
    pyrrolysyl-tRNA
    

    Tags

    none

    Expression

    Bacterial

    Mutation

    mutated Asparagine 166 to Alanine and Valine 168 …

    Promoter

    proK-lacO and tacI

    Available Since

    June 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDONR223_TRAF5_WT

    Plasmid

    #82167

    Purpose

    Gateway Donor vector containing TRAF5 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    TRAF5 (TRAF5 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. nAChR beta2 YFP

    Plasmid

    #15107

    Depositor

    Henry Lester

    Article

    Nashmi et al J Neurosci. 2003 Dec 17. 23(37):11554-67

    Insert

    beta2-YFP-M (Chrnb2 Mouse)
    

    Tags

    YFP

    Expression

    Mammalian

    Available Since

    June 8, 2007

    Availability

    Academic Institutions and Nonprofits only