We narrowed to 2,549 results for: PORA
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Plasmid#226202PurposeElectroporation knockout plasmid (pK18sB) to delete the C. necator soluble hydrogenase operon (deltaSH).DepositorInsertHomology arms for soluble hydrogenase operon
UseSynthetic BiologyMutation∆hoxFUYHWI ∆hypA2B2F2Available SinceNov. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCHC023 (∆MBH)
Plasmid#226201PurposeElectroporation knockout plasmid (pK18sB) to delete the C. necator membrane-bound hydrogenase operon (deltaMBH).DepositorInsertHomology arms for membrane bound hydrogenase operon
UseSynthetic BiologyMutation∆hoxKGZMLOQRTV ∆hypA1B1F1CDEX ∆hoxABCJAvailable SinceNov. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCHC004 (∆phaCAB)
Plasmid#226198PurposeElectroporation knockout plasmid (pK18sB) to delete the C. necator phaCAB operon, to prevent accumulation of PHB.DepositorInsertHomology arms for phaCAB
UseSynthetic BiologyAvailable SinceNov. 15, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::B1-NLSLacZ-B2
Plasmid#186413PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined NLSLacZ under control of an AttB3/B5 recombined reg. sequence.DepositorAvailable SinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
RGD-I27-CC
Plasmid#85393PurposeMTFM mechanosensor for integrin. Integrin binding domain fused to titan immunoglobulin domain (I27) with two cysteines for immobilization and site for p-azidophenylalanine incorporation & Cy3 labelingDepositorInsertRGD motif for integrin binding fused to titin immunoglobulin domain (I27), containing a TAG (amber) codon
Tags6x HisExpressionBacterialPromoterT7Available SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
NRF2-SmBiT_MAFG-LgBiT BiBiT Vector
Plasmid#238773PurposeExpress NRF2-SmBiT_MAFG-LgBiT Fusion Protein in Mammalian Cells under a CMV promoterDepositorHas ServiceDNATagsLgBiT and SmBiTExpressionMammalianMutationNonePromoterCMVAvailable SinceSept. 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pANAP-P2A-eRF(E55D)
Plasmid#241290PurposeAllows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses tRNACUA-Leu, AnapRS, and dominant negative ribosomal release factorDepositorInsertthe amber suppressor tRNACUA-Leu
ExpressionMammalianMutationmutant E.coli leucyl synthetase specific for Anap…PromoterCMVAvailable SinceDec. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-neo-CD2-FAK
Plasmid#37013DepositorAvailable SinceOct. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-AntiHer2-LC122-TAG
Plasmid#226839PurposeContains trastuzumab with a TAG codon at position 122 of the light chain for ncAA incorporationDepositorInsertsanti-HER2 light chain
anti-HER2 heavy chain
UseAffinity Reagent/ AntibodyExpressionMammalianMutationamino acid 122 of light chain mutated to TAGAvailable SinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-AntiHer2-HC197-TAG
Plasmid#226838PurposeContains trastuzumab with a TAG codon at position 197 of the heavy chain for ncAA incorporationDepositorInsertsanti-HER2 light chain
anti-HER2 heavy chain
UseAffinity Reagent/ AntibodyExpressionMammalianMutationamino acid 197 of heavy chain mutated to TAGAvailable SinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
XLone-Puro Cas13d-eGFP U6 BbsI
Plasmid#155184PurposePiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and U6 gRNA backboneDepositorTypeEmpty backboneUseUnspecified; Piggybac transposonExpressionMammalianPromoterU6Available SinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-ABK
Plasmid#126035PurposeEfficient expression of tRNA synthetase/tRNA for the incorporation of a photo-cross-linker, 3’-azibutyl-N-carbamoyl-lysine (AbK) into recombinant protein by amber codon (TAG) suppression in E. coli.DepositorInsertstRNA synthetase 1
tRNA synthetase 2
tRNA for TAG codon
ExpressionBacterialMutationL274M, C313A, Y349FPromoteraraBAD promoter, glnS promoter, and proK promoterAvailable SinceSept. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDule-3-nitroTyrosine (5B)
Plasmid#85498PurposePlasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsert3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
TagsNoneExpressionBacterialMutationY32H H70C D158S I159A L162RPromoterlpp (constitutive)Available SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDule-Tet2.0
Plasmid#85496PurposePlasmid for incorporating the non-canonical amino acid Tetrazine 2.0 with the Mj Tet2.0 synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsertTetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
TagsNoneExpressionBacterialMutationY32G L65Q F108S Q109D D158S L162NPromoterlpp (constitutive)Available SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSF-MjCouRS
Plasmid#229066PurposetRNA synthetase/tRNA pair for the in vivo incorporation of (7-hydroxy-4-coumarin-yl) ethylglycine (Hco), into proteins in E. coli in response to the amber (TAG) codonDepositorInsertMethanococcus jannaschii tRNA synthetase
ExpressionBacterialPromoterT7Available SinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1.tR4-AcdRS82
Plasmid#174101PurposeExpression of orthogonal Mb tRNA and Acd82 tRNA synthetase for incorporation of acridone in eukaryotic cells.DepositorInsertM. barkeri tRNA synthetase for acridone82
ExpressionMammalianMutationN311S, C313G, V366A, W382TPromoterCMVAvailable SinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDule2-Tet2.0
Plasmid#85497PurposePlasmid for incorporating the non-canonical amino acid Tetrazine 2.0 with the Mj Tet2.0 synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsertTetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
ExpressionBacterialMutationY32G L65Q F108S Q109D D158S L162NPromoterlpp (constitutive)Available SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV CytoBAS
Plasmid#62860PurposeFluorescent bile acid sensor (BAS) that allows spatiotemporal monitoring of bile acid transport in single living cells using FRETDepositorInsertCytoBAS
ExpressionMammalianPromoterCMVAvailable SinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV NucleoBAS
Plasmid#62861PurposeFluorescent bile acid sensor (BAS) targeted to nucleus that allows spatiotemporal monitoring of bile acid transport in single living cells using FRETDepositorInsertNucleoBAS
TagsSV40 NLSExpressionMammalianPromoterCMVAvailable SinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDule2-3-nitroTyrosine (5B)
Plasmid#85499PurposePlasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsert3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
ExpressionBacterialMutationY32H H70C D158S I159A L162RPromoterlpp (constitutive)Available SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only