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pDRF1 Cyterm-yemiRFP670

Plasmid

#219856

Purpose

expression of Cyterm-yemiRFP670 in budding yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

Cyterm-yemiRFP670

Expression

Yeast

Available Since

Aug. 27, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pDRF1 Cyterm-ymiRFP680

Plasmid

#219854

Purpose

expression of Cyterm-ymiRFP680 in budding yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

Cyterm-ymiRFP680

Expression

Yeast

Available Since

Aug. 27, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pDRF1-GW ymTq2-ymScarletI

Plasmid

#205512

Purpose

Expression of ymTq2-ymScarletl in yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

ymTq2-ymScarletl

Expression

Yeast

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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Cyterm-mAvicFP1 pDRF1-GW

Plasmid

#168092

Purpose

Expresses Cyterm-mAvicFP1 to check for monomericity.

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

mAvicFP1

Expression

Yeast

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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Cyterm-ystagRFP pDRF1-GW

Plasmid

#168061

Purpose

Expresses Cyterm-ystagRFP to check for monomericity.

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

Cyterm-ystagRFP

Expression

Yeast

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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Cyterm-AausFP1 pDRF1-GW

Plasmid

#168062

Purpose

Expresses Cyterm-AausFP1 to check for monomericity.

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

Cyterm-AausFP1

Expression

Yeast

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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MvChR1_EYFP_pcDNA3.1

Plasmid

#159119

Purpose

This plasmid encodes MvChR1 rhodopsin domain

Depositor

John Spudich

Article

Govorunova et al mBio. 2011 Jun 21;2(3):e00115-11. doi: 1

Insert

MvChR1

Tags

EYFP

Expression

Mammalian

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAF302

Plasmid

#137752

Purpose

Intracellular control plasmid for HiBiTopt experiments

Depositor

Wiep Klaas Smits

Article

Oliveira Paiva et al J Bacteriol. 2020 Aug 31. pii: JB.00771-

Insert

hupA-linker-hibitopt

Tags

HiBiTopt

Expression

Bacterial

Promoter

Ptet

Available Since

Sept. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pAP233

Plasmid

#137753

Purpose

Extracellular control for HiBiTopt experiments

Depositor

Wiep Klaas Smits

Article

Oliveira Paiva et al J Bacteriol. 2020 Aug 31. pii: JB.00771-

Insert

srtB-linker-hibitopt

Use

Luciferase

Tags

HiBiTopt

Expression

Bacterial

Promoter

Ptet

Available Since

Sept. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pIAGO-Hap2

Plasmid

#114460

Purpose

Expression of hybrid AmphDI-PerDI (Hap) glycosyltansferase and perosamine synthase in streptomycetes

Depositor

Patrick Caffrey

Article

Hutchinson et al Chem Biol. 2010 Feb 26;17(2):174-82. doi

Insert

Hap2 + perDII

Expression

Bacterial

Promoter

ermE

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pJK599

Plasmid

#72524

Purpose

Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits, and associated hypothetical protein (AaPurS, AaOrfY, AaPurQ, AaPurLH6)

Depositor

T. J. Kappock

Inserts

formylglycinamidine ribonucleotide synthetase, small subunit
hypothetical protein in purSQL operon
formylglycinamidine ribonucleotide synthetase, glutaminase subunit
formylglycinamidine ribonucleotide synthetase, large subunit

Expression

Bacterial

Mutation

synthetic gene

Promoter

T7 and n/a

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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pJK618

Plasmid

#72535

Purpose

Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit, N-term His6 tag; glutaminase subunit; large subunit, C-terminal His6 tag (H6AaPurS, AaPurQ, AaPurLH6)

Depositor

T. J. Kappock

Inserts

formylglycinamidine ribonucleotide synthetase, small subunit
formylglycinamidine ribonucleotide synthetase, glutaminase subunit
formylglycinamidine ribonucleotide synthetase, large subunit

Tags

His6

Expression

Bacterial

Mutation

synthetic gene

Promoter

T7 and n/a

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pgRNA-bacteria

Plasmid

#44251

Purpose

Expression of customizable guide RNA (gRNA) for bacterial gene knockdown

Depositor

Stanley Qi

Article

Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

Insert

guide RNA (bacteria)

Use

CRISPR

Expression

Bacterial

Promoter

BBa_J23119 (SpeI)

Available Since

April 5, 2013

Availability

Academic Institutions and Nonprofits only

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PH-PLCD1-GFP

Plasmid

#51407

Purpose

Is a biosensor for PI(4,5)P2

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

Insert

PH-PLCD1 (aa 1–170) (PLCD1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pKS1

Plasmid

#84079

Purpose

Expresses the non-toxic fragment C of tetanus toxin

Depositor

Neil Fairweather

Article

Sinha et al Mol Microbiol. 2000 Sep;37(5):1041-51.

Insert

Tetanus neurotoxin fragment C

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2018

Availability

Academic Institutions and Nonprofits only

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pSTARR-seq_fly

Plasmid

#71499

Purpose

flySTARR-seq screening vector with DSCP core promoter.

Depositor

Alexander Stark

Article

Arnold et al Science. 2013 Mar 1;339(6123):1074-7. do

Type

Empty backbone

Use

Flystarr-seq screening vector

Tags

sgGFP (SuperGlo, Qbiogene, Inc)

Expression

Insect

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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PH-Akt(R25C)-GFP

Plasmid

#51466

Purpose

Non-PIP3 binding control for PH-Btk-GFP

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

Insert

AKT (aa 1–164)

Tags

EGFP

Expression

Mammalian

Mutation

R25C

Promoter

CMV

Available Since

March 26, 2014

Availability

Academic Institutions and Nonprofits only
pDRF1 ymBaojin-T2A-mCherry

Plasmid

#219857

Purpose

equimolar expression of ymBaojin and mCherry in budding yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

ymBaojin-T2A-mCherry

Expression

Yeast

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pET21a.Tat(48-57)-EGFP

Plasmid

#165040

Purpose

Expression of Tat(48-57) conjugated EGFP for protein purification (6XHis tag)

Depositor

Dokyun Na

Article

Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

Insert

EGFP

Tags

6XHis and Tat(48-57)

Expression

Bacterial

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-TrioN

Plasmid

#129621

Purpose

RhoGEF

Depositor

Dorus Gadella

Article

Reinhard et al Small GTPases. 2019 Aug 30:1-15. doi: 10

Insert

N-terminal part of TRIO

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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