We narrowed to 9,264 results for: Coli

1. pRSFDuet-1_DARPinOFF7

   Plasmid

   #182396

   Purpose

   Bacterial expression of DARPinOFF7

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   DARPinOFF7
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRXG

   Plasmid

   #113643

   Purpose

   This construct contains an amber stop codon within the linker between the two genes. The read through rate of the amber stop codon can be determined using the ratio of GFP signal to RFP signal.

   Depositor

   Jeffrey Barrick

   Article

   Monk et al ACS Synth Biol. 2016 Sep 20.

   Insert

   mRFP1-linker-sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAJT248

   Plasmid

   #202289

   Purpose

   RPU integration plasmid (LPattB2)

   Depositor

   Christopher Voigt

   Article

   Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

   Insert

   J23101-RiboJ00-B0034-YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 FLIPE-1m

   Plasmid

   #13544

   Depositor

   Wolf Frommer

   Article

   Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

   Insert

   FLIP-E A207W
   

   Use

   Tags

   ECFP and Venus

   Expression

   Mammalian

   Mutation

   A207W in binding protein

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSET FLIPE intramol-1u

   Plasmid

   #13549

   Depositor

   Wolf Frommer

   Article

   Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

   Insert

   FLIP-E intramol WT (gltI E. coli)
   

   Use

   Tags

   EYFP

   Expression

   Bacterial

   Mutation

   ECFP insert in binding protein

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDisplay FLIPE-600n

   Plasmid

   #13545

   Depositor

   Wolf Frommer

   Article

   Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

   Insert

   FLIP-E WT
   

   Use

   Tags

   ECFP and Venus

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-EF1a-PKAsub-mCherry-MinD-IRES-Puro

   Plasmid

   #213118

   Purpose

   Expression of one half of a PKAsub-mCherry-MinD reporter circuit in mammalian cells

   Depositor

   Scott Coyle

   Article

   Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

   Insert

   PKAsub-mCherry-MinD (minD Escherichia coli)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. cre_CD

   Plasmid

   #66030

   Purpose

   MoClo Basic Part: CDS - cre recombinase (recombines DNA at loxP sites) [C:cre:D]

   Depositor

   Douglas Densmore

   Article

   Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

   Insert

   Recombinase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCB11

   Plasmid

   #201111

   Purpose

   pSR58.6 (Addgene plasmid #63176) modified to include the tetA gene upstream of sfGFP.

   Depositor

   Mary Dunlop

   Article

   Lugagne et al Nat Commun. 2024 Mar 8;15(1):2148. doi:

   Inserts

   CcaR
   tetA(C)
   sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C-to-T transition at pos. no. 1049 of tetA(C) lea…

   Promoter

   J23100 and PcpcG2-172

   Available since

   March 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. plYJP067-Ptet

    Plasmid

    #202299

    Purpose

    PTet-YFP plasmid for LPattB2 integration

    Depositor

    Christopher Voigt

    Article

    Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

    Insert

    Ptet-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-LplA(W37I) Alternative coumarin PRIME ligase

    Plasmid

    #25846

    Depositor

    Alice Ting

    Article

    Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

    Insert

    LplA-W37I
    

    Use

    Tags

    Flag and His

    Expression

    Mammalian

    Mutation

    W37I

    Promoter

    Available since

    July 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFLIPglu-2uDelta13

    Plasmid

    #12990

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

    Insert

    FLIPglu F16F D152A Delta13
    

    Use

    Plant nanosensor

    Tags

    ECFP and EYFP

    Expression

    Plant

    Mutation

    D152A mutation in binding protein

    Promoter

    Available since

    Jan. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSNA

    Plasmid

    #135192

    Purpose

    Arabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains ampicillin resistance cassette

    Depositor

    Hongqing Fang

    Article

    Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

    Insert

    I-SceI lamba Red
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    araBAD

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLEX-uORF-HA-birA*-STOP-IRES-Puro

    Plasmid

    #120558

    Purpose

    Expresses control HA-birA* in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    birA
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    R118G

    Promoter

    CMV

    Available since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1 FLIPE-100u

    Plasmid

    #13543

    Depositor

    Wolf Frommer

    Article

    Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

    Insert

    FLIP-E S118W
    

    Use

    Tags

    ECFP and Venus

    Expression

    Mammalian

    Mutation

    S118W in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFLIPglu-600uDelta13

    Plasmid

    #12991

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

    Insert

    FLIPglu F16A Delta13
    

    Use

    Plant nanosensor

    Tags

    ECFP and EYFP

    Expression

    Plant

    Mutation

    F16A in binding protein

    Promoter

    Available since

    Jan. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pMBA323

    Plasmid

    #214751

    Purpose

    To create delta(glmS,nadE) working strains and maintaining their viability

    Depositor

    Tae Seok Moon

    Article

    Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

    Insert

    BBa_J23105-cas9 + pBAD-λ Red genes + ptet-gRNA (pBR322ori)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAJT430

    Plasmid

    #202291

    Purpose

    RPU integration plasmid (LPattB7)

    Depositor

    Christopher Voigt

    Article

    Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

    Insert

    J23101-RiboJ00-B0034-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. J23103_GB

    Plasmid

    #65991

    Purpose

    MoClo Basic Part: Constitutive promoter - Anderson series - low strength [G:J23103:B]

    Depositor

    Douglas Densmore

    Article

    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

    Insert

    Constitutive promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. J23107_GB

    Plasmid

    #65999

    Purpose

    MoClo Basic Part: Constitutive promoter - Anderson series - medium strength [G:J23107:B]

    Depositor

    Douglas Densmore

    Article

    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

    Insert

    Constitutive promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. J23116_GB

    Plasmid

    #66003

    Purpose

    MoClo Basic Part: Constitutive promoter - Anderson series - low strength [G:J23116:B]

    Depositor

    Douglas Densmore

    Article

    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

    Insert

    Constitutive promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTU2S-a (colE1 origin)

    Plasmid

    #74091

    Purpose

    Secondary module (BpiI) site in pTU2-a backbone with colE1 (medium-copy) origin of replication

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Golden Gate destination vector - Level 2, 2 TU's with secondary module and colE1 origin
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    None

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAJT254-litR

    Plasmid

    #202308

    Purpose

    litR repressor plasmid for LPattB2 integration

    Depositor

    Christopher Voigt

    Article

    Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

    Insert

    litR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAJT249-psrA

    Plasmid

    #202304

    Purpose

    psrA repressor plasmid for LPattB2 integration

    Depositor

    Christopher Voigt

    Article

    Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

    Insert

    psrA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRJPaph-lacZYA

    Plasmid

    #67037

    Purpose

    Plasmid for Paph-lacZYA integration downstream of B. diazoefficiens (B. japonicum) scoI

    Depositor

    Julia Vorholt

    Article

    Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

    Inserts

    B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
    lacZYA
    

    Use

    Conjugative bacterial vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET22b-BirA-CPDSalI

    Plasmid

    #38258

    Depositor

    Aimee Shen, Matthew Bogyo

    Article

    Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

    Insert

    birA (birA E. coli)
    

    Use

    Tags

    6xHis and Vibrio cholerae MARTX toxin cysteine pr…

    Expression

    Bacterial

    Mutation

    cloned from pGEX4T1-BirA

    Promoter

    T7

    Available since

    July 20, 2012

    Availability

    Academic Institutions and Nonprofits only

27. pTH

    Plasmid

    #56659

    Purpose

    Dual-expression vector for P. tricornutum

    Depositor

    Philip Weyman

    Article

    Weyman et al Plant Biotechnol J. 2014 Oct 10. doi: 10

    Insert

    KanR
    

    Use

    Synthetic Biology; Diatom expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pT7-agrBD-I

    Plasmid

    #53439

    Purpose

    T7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220

    Depositor

    Cynthia Collins

    Article

    Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

    Insert

    agrBD locus
    

    Use

    Synthetic Biology

    Tags

    V5 epitope, 6x HIS (not expressed on agrB or agrD…

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7-lac

    Available since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. T7 promoter + rpsL reporter plasmid

    Plasmid

    #156455

    Purpose

    A mutational reporter plasmid that can gain Streptomycin and/or Tetracycline resistance upon mutation. Contains T7 promoter.

    Depositor

    Matthew D. Shoulders

    Article

    Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

    Inserts

    rpsL
    TcR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    ATG start codon mutated to ACG

    Promoter

    tac and tet

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRJPaph-gusA

    Plasmid

    #67036

    Purpose

    Plasmid for Paph-gusA integration downstream of B. diazoefficiens (B. japonicum) scoI

    Depositor

    Julia Vorholt

    Article

    Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

    Inserts

    B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
    gusA
    

    Use

    Conjugative bacterial vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge