We narrowed to 7,476 results for: lif

1. pKrox24(2xD-E)dTomato

   Plasmid

   #200110

   Purpose

   Fluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   modified EGR1 promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   highly modified sequence

   Promoter

   2 copies of D-E element, no other promoter elemen…

   Available since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV.CAG.Flex.NES-jRCaMP1b.WPRE.SV40

   Plasmid

   #100849

   Purpose

   AAV expression of Cre recombinase-activated jRCaMP1b, a red fluorescent calcium sensor protein, from the CAG promoter

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Has Service

   AAV1

   Insert

   jRCaMP1b
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pXW117As(RS-E11-RinA)2

   Plasmid

   #123146

   Purpose

   Arsenic sensor with three-layered amplifier cascade. Need to work with plasmid pXWPrinA-gfp2. pSB4A3 carrying J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-33rinA-t

   Depositor

   Baojun Wang

   Article

   Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

   Insert

   J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-33rinA-t
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J117, ParsR, PhrpLE, Pecf11

   Available since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV.Syn.Flex.NES-jRCaMP1b.WPRE.SV40

   Plasmid

   #100850

   Purpose

   AAV expression of Cre recombinase-activated jRCaMP1b, a red fluorescent calcium sensor protein, from the Synapsin promoter

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Has Service

   AAV1

   Insert

   jRCaMP1b
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   March 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNS20-SpCas9-SNAP

   Plasmid

   #113717

   Purpose

   Bacterial vector for expression of Snap-tagged Streptococcus pyogenes Cas9

   Depositor

   Gerald Schwank

   Article

   Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   HA-2xNLS-SNAP-NLS and His6-MBP-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-RYK-V5/HIS

   Plasmid

   #205610

   Purpose

   Expression of human RYK receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   RYK receptor tyrosine kinase (RYK Human)
   

   Use

   Tags

   V5-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPB-3xnls-Tq-Ca-FLITS

   Plasmid

   #145030

   Purpose

   piggyBac vector for expressing a Turquoise calcium sensor (GECI) that reports with lifetime and intensity changes

   Depositor

   Dorus Gadella

   Article

   van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

   Insert

   3xnls-Tq-Ca-FLITS
   

   Use

   Tags

   3xnls-Tq-Ca-FLITS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1/Puro-CAG-ASAP2s

   Plasmid

   #101274

   Purpose

   Genetically encoded voltage indicator ASAP2s expressed under strong mammalian promoter (CAG)

   Depositor

   Francois St-Pierre

   Article

   Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

   Insert

   ASAP2s
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKrox24(2xD-E_inD)Luc

   Plasmid

   #200109

   Purpose

   Luciferase reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   modified EGR1 promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   highly modified sequence

   Promoter

   2 copies of D-E element in front of EGR1 promoter…

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Nrp1-Fc-His

    Plasmid

    #72097

    Purpose

    Expresses the extracellular region of the Neuropilin 1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Nrp1 (Nrp1 Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCB’ CbbL Y72R

    Plasmid

    #162707

    Purpose

    Carboxysome operon with point mutation to CbbL, prk; Substitution of CsoS2-binding tyrosine with arginine prevents rubisco encapsulation creating rubisco-less carboxysomes

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    pHnCB10 derived carboxysome operon, prk
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    CbbL Y72R; Substitution of CsoS2-binding tyrosine…

    Promoter

    PLtet0-1 promoter

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO.1-shSNAI1

    Plasmid

    #115467

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    shSNAI1 (SNAI1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHalo-MyosinIIA-C25

    Plasmid

    #128573

    Purpose

    Studies of cellular contractility

    Depositor

    Dylan Burnette

    Article

    Fenix et al Elife. 2018 Dec 12;7. pii: 42144. doi: 1

    Insert

    Myosin IIA (Myh9 Mouse)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSMT3_SRSF1

    Plasmid

    #201056

    Purpose

    Express sumo-tagged human SRSF1

    Depositor

    Jun Zhang

    Article

    Fargason et al Elife. 2023 Mar 2;12:e84412. doi: 10.755

    Insert

    SRSF1 (SRSF1 Human)
    

    Use

    Tags

    sumo

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMIA3-Olônne

    Plasmid

    #214447

    Purpose

    Expresses eSpCas9 and gRNA targeting Olônne-18 GSH

    Depositor

    Roger Foo

    Article

    Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

    Insert

    eSpCas9-P2A-mRuby2-P2A-tp53dn
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBeta-lacZ-attBx2

    Plasmid

    #124445

    Purpose

    Plasmid for in vivo enhancer reporter assay. Plasmid contains a β-globin minimal promoter followed by a lacZ reporter gene derived from pRS16, with the entire reporter cassette flanked by attB sites.

    Depositor

    Yingguang Frank Chan

    Article

    Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

    Type

    Empty backbone

    Use

    Enhancer reporter assay construct

    Tags

    LacZ

    Expression

    Mammalian

    Mutation

    Promoter

    beta globin minimal promoter

    Available since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV HA hRB delta CDK + T373

    Plasmid

    #58912

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T373 restored

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CDK* with T373 restored

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pNS38-SadCas9-SNAP

    Plasmid

    #113718

    Purpose

    Bacterial vector for expression of Snap-tagged Staphylococcus aureus dCas9

    Depositor

    Gerald Schwank

    Article

    Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    HA-2xNLS-SNAP-NLS and His6-MBP-TEV

    Expression

    Bacterial

    Mutation

    D10A, N580A

    Promoter

    Available since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. Sema7a-Fc-His

    Plasmid

    #72175

    Purpose

    Expresses the extracellular region of the Sema7A protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema7a (Sema7a Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. Fireworks RFP[PTC+] (AVA2626)

    Plasmid

    #85444

    Purpose

    Expresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a PTC-containing beta-globin reporter.

    Depositor

    Joan Steitz

    Article

    Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:

    Insert

    tDNA1 EF1alfa-5xtdTomato-TEVprotease-PEST-beta-globin(dI1)(PTC39)-BGH polyA tDNA2 FRT-HygromycinR
    

    Use

    Minimal backbone fragment for low-copy bacterial …

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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