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Showing: 2461 - 2480 of 2760 results

  1. pGBW-m4046938

    Plasmid
    #145599
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145599-plasmid-map-sequence-id-300265

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  2. pGL5

    Plasmid
    #48745
    Purpose
    Expression of luciferase driven by truncated mPer2 promoter region (bases -1128 to +2064 with respect to transcription start site at +1)
    Depositor
    Joseph Takahashi
    Article
    Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10
    Insert
    truncated mPer2 promoter/enhancer region (-1128 to +2064)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    truncated promoter region (see pGL6)
    Promoter
    Available since
    Dec. 19, 2013
    Availability
    Academic Institutions and Nonprofits only

  3. RAB14 3'UTR D1D2

    Plasmid
    #61492
    Purpose
    luciferase reporter containing RAB14 3' UTR with both predicted MiR144 binding site deleted
    Depositor
    Curt Civin
    Article
    Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111
    Insert
    RAB14 3' UTR (RAB14 Human)
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mammalian
    Mutation
    contains position 837-1637 (numbering relative to…
    Promoter
    PGK
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only

  5. pGBW-m4046831

    Plasmid
    #145671
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;Myc
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145671-plasmid-map-sequence-id-319574

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  6. pHL 1405

    Plasmid
    #53010
    Purpose
    pLtetO-1:RhyB, pCon:DsrA, pLtetO-1:hfq, pLlacO-1:sodB::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    RhyB sRNA
    DsrA sRNA
    hfq
    sodB
    Use
    Synthetic Biology
    Tags
    gfp
    Expression
    Bacterial
    Mutation
    contains -56 to +141 relative to start codon
    Promoter
    pCon, pLlacO-1, and pLtetO-1
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  7. pGBW-m4046970

    Plasmid
    #145753
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;HA
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145753-plasmid-map-sequence-id-311539

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  9. RAB14 3'UTR D1

    Plasmid
    #61490
    Purpose
    luciferase reporter containing RAB14 3' UTR with the first predicted MiR144 binding site deleted
    Depositor
    Curt Civin
    Article
    Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111
    Insert
    RAB14 3' UTR (RAB14 Human)
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mammalian
    Mutation
    contains position 837-1637 (numbering relative to…
    Promoter
    PGK
    Available since
    Jan. 9, 2015
    Availability
    Academic Institutions and Nonprofits only

  11. RAB14 3'UTR D2

    Plasmid
    #61491
    Purpose
    luciferase reporter containing RAB14 3' UTR with the second predicted MiR144 binding site deleted
    Depositor
    Curt Civin
    Article
    Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111
    Insert
    RAB14 3' UTR (RAB14 Human)
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mammalian
    Mutation
    contains position 837-1637 (numbering relative to…
    Promoter
    PGK
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only

  12. pHRIG-S6K

    Plasmid
    #59446
    Purpose
    lentiviral expression of S6K and EGFP
    Depositor
    Heng Zhao
    Article
    Xie et al Stroke. 2014 Jul 10. pii: STROKEAHA.114.
    Insert
    S6K (Rps6kb1 Rat)
    Use
    Lentiviral
    Tags
    IRES-EGFP
    Expression
    Mammalian
    Mutation
    S6K starts at aa24 and contains 7 extra amino aci…
    Promoter
    CMV
    Available since
    Oct. 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. EW411 (UTRN-43-5bp spacer-UTRN-14)-H2B-GFP

    Plasmid
    #236148
    Purpose
    Fluorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with the UTRN promoter region encompassing 14 to 67 bp upstream of its transcriptional start site
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al bioRxiv 2025.02.24.639918
    Insert
    H2B (H2BC11 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    E1b minimal promoter with the UTRN promoter region
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236148-plasmid-map-sequence-id-468443

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  14. SCN1Aprom(1-500)-H2B-GFP

    Plasmid
    #236162
    Purpose
    Fluorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with the SCN1a promoter region encompassing 500 bp upstream of its transcriptional start site
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al bioRxiv 2025.02.24.639918
    Insert
    H2B (H2BC11 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    E1b minimal promoter with the SCN1a promoter regi…
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236162-plasmid-map-sequence-id-468412

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  15. SCN1Aprom(335-406)-H2B-GFP

    Plasmid
    #236151
    Purpose
    Fluorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with the SCN1a promoter region encompassing 335 to 406 bp upstream of its transcriptional start site
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al bioRxiv 2025.02.24.639918
    Insert
    H2B (H2BC11 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    E1b minimal promoter with the SCN1a promoter regi…
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236151-plasmid-map-sequence-id-468384

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  16. pCAGGS-Twin-strep-TEV-DGKδ2

    Plasmid
    #223720
    Purpose
    Express human diacylglycerol kinase delta 2 protein (N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell
    Depositor
    Chiaki Murakami
    Insert
    DGKD (DGKD Human)
    Use
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Mammalian
    Mutation
    deleted start codon
    Promoter
    CAG and chicken β-actin promoter
    Available since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    223720-plasmid-map-sequence-id-457431

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  17. p2H12ref

    Plasmid
    #221188
    Purpose
    cdGreen expressed in operon with mScarlet-I from an aTc-inducible promoter (Internal Lab ID: UJ11206)
    Depositor
    Urs Jenal
    Article
    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:
    Inserts
    cdGreen
    mScarlet-I
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N-terminus changed: starts with MSKKYGEAVIKE ...
    Promoter
    None (same as cdGreen) and Ptet
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    221188-plasmid-map-sequence-id-437182

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Showing: 2461 - 2480 of 2760 results