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Plasmid#162966PurposeGateway attB5r/attB1r entry clone for generation of N-terminal dual HA-tagged fusion proteinsDepositorInsertDual HA epitope tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGMR-WhiteA2
Plasmid#165874PurposeBasic transgenesis 5xGMR white CDS physical eye marker cassette in Alpha2, lacks attB siteDepositorInsertEye Marker Cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPuroR-GMRWhite
Plasmid#165884PurposePuromycin resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screeningDepositorInsertTransgenesis Ready Selection Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-BsmBI-HA_IRES_BSD
Plasmid#162805PurposeSTELLA destination plasmid with HA terminal tag; Suitable for amber suppression (4xMmaPylT cassette); for transient or stable piggyBac-mediated integrationDepositorInsertUb-BsmBI-HA_IRES_Blasticidin
UsePiggybacTagsHAExpressionMammalianMutationPromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWDY1226
Plasmid#138593PurposeExpresses NatL2-R156A in E.coliDepositorInsertphenylacetate-coenzyme A ligase
UseTags6-HisExpressionBacterialMutationchanged Arginine 156 to AlaninePromoterT7Available sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWDY1222
Plasmid#138592PurposeExpresses NatL2-K418A in E.coliDepositorInsertphenylacetate-coenzyme A ligase
UseTags6-HisExpressionBacterialMutationchanged Lysine 418 to AlaninePromoterT7Available sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMET17p-CCG 316
Plasmid#131166PurposeFor ScMET17 promoter regulated expression of mCherry-Cub-R-GFP (CCG) fused bait proteins, centromeric ARS plasmid, URA3 complements the uracil auxotrophy of S. cerevisiae ura mutantDepositorTypeEmpty backboneUseTagsCCGExpressionYeastMutationPromoterAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS_KS_attB2_SA(2)_T2AGAL80
Plasmid#125219PurposeArtificial phase 2 exon to include a spliced T2A-GAL80 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a geneDepositorInsertGAL80
UseOtherTagsT2AExpressionMutationPromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYC_HA-ZmSAE1_ZmSAE2(TRUNC)-FLAG
Plasmid#91945PurposeBacterial expression of maize SAE1 and truncated SAE2DepositorInsertsHA-ZmSAE1
ZmSAE2-FLAG
UseTagsFLAG and HAExpressionBacterialMutationTruncation of ZmSAE2 to mimic a splice variantPromoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNCMV HPV8 E6 R138S
Plasmid#87659PurposeFlag/HA tagged HPV8 E6 R138SDepositorInsertHPV 8E6
UseTagsFlag and HAExpressionMammalianMutationR138SPromoterCMVAvailable sinceJune 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNCMV HPV8 E6 K64A
Plasmid#87656PurposeFlag/HA tagged HPV8 E6 K64ADepositorInsertHPV 8E6
UseTagsFlag and HAExpressionMammalianMutationK64APromoterCMVAvailable sinceMarch 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNCMV HPV8 E6 K136N
Plasmid#87657PurposeFlag/HA tagged HPV8 E6 K136NDepositorInsertHPV 8E6
UseTagsFlag and HAExpressionMammalianMutationK136NPromoterCMVAvailable sinceMarch 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNCMV HPV8 E6 K142A
Plasmid#87660PurposeFlag/HA tagged HPV8 E6 K142ADepositorInsertHPV 8E6
UseTagsFlag and HAExpressionMammalianMutationK142APromoterCMVAvailable sinceMarch 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNCMV HPV8 E6 L59D
Plasmid#87655PurposeFlag/HA tagged HPV8 E6 L59DDepositorInsertHPV 8E6
UseTagsFlag and HAExpressionMammalianMutationL59DPromoterCMVAvailable sinceMarch 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
NCMV MmuPV1 E6 R52A
Plasmid#87678PurposeFlag/HA tagged MmuPV1 E6 R52ADepositorInsertMmuPV1 E6
UseLentiviralTagsFlag and HAExpressionMutationR52APromoterCMVAvailable sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNCMV MmuPV1 E6 R130A
Plasmid#87654PurposeFlag/HA tagged MmuPV1 E6 R130ADepositorInsertMmuPV1 E6
UseTagsFlag and HAExpressionMammalianMutationR130APromoterCMVAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNCMV MmuPV1 E6
Plasmid#87653PurposeFlag/HA tagged MmuPV1 E6DepositorInsertMmuPV1 E6
UseTagsFlag and HAExpressionMammalianMutationPromoterCMVAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
P. falciparum DHPS
Plasmid#64080PurposeExpression of P. falciparum dihydropteroate synthaseDepositorInsertdihydropteroate synthase
UseTagsHistidine tagExpressionMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
S. aureus DHPS
Plasmid#64079PurposeExpression of Staphylococcus aureus dihydropteroate synthaseDepositorInsertdihydropteroate synthase
UseTagsHistidine tagExpressionBacterialMutationPromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
TM1442-pET-21a
Plasmid#12650DepositorInsertPutative anti-sigma factor antagonist (TM1442 T. maritima)
UseTagsExpressionBacterialMutationN/APromoterAvailable sinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCS2HA
Plasmid#16330DepositorInsertHA tag
UseVertebrate expressionTagsExpressionMutationPromoterAvailable sinceDec. 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRS426-HsFECH
Plasmid#174524PurposeExpresses human ferrochelatase (FECH) in yeastDepositorInsertFECH with yeast HEM15 promoter and terminator (FECH Human, Budding Yeast, Synthetic)
UseTagsExpressionYeastMutationLacks original mitochondrial targeting sequence (…PromoterHEM15 (yeast, 257 bp)Available sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAG-LoxpStopLoxp-NLS-dCas9-HA-NLS-NLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-T2A-EGFP-WPRE-PolyA
Plasmid#107307PurposeEncoding an SPH activation systemDepositorInsertCAG-LSL-dCas9-10xGCN4-P2A-scFv-P65-HSF1-T2A-EGFP-WPRE-PolyA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-YFP-PKD1
Plasmid#31527DepositorInsertYFP-HA-Protein Kinase D (PRKD1 Human)
UseTagsHA and YFPExpressionMammalianMutationPromoterAvailable sinceNov. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
VR124 pETv2 10His-pG-Tn5 (E54K, L372P)
Plasmid#198468PurposeE. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in T7 Express lysY/Iq protein expression strain.DepositorInsert10His-pG-Tn5 (E54K, L372P)
UseTags10His-pGExpressionBacterialMutationE54K, L372PPromoterConsensus φ10 T7 PromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMVHA SUZ12
Plasmid#24232PurposeMammalian expression of human SUZ12 with HA tagDepositorInsertSUZ12 (SUZ12 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceDec. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-GFP
Plasmid#86537PurposeRetrovirus expressing GFPDepositorInsertGFP
UseRetroviralTagsExpressionMammalianMutationPromoterSynapsin I (human)Available sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
MAC-tag-N
Plasmid#108078PurposeGateway compatible destination vectors containing StrepIII/HA/BioIDDepositorInsertCCDB (ccdB(letD) )
UseTagsStrepIII-HA-BirA*ExpressionBacterial and MammalianMutationPromoterAvailable sinceApril 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
T7RNAP Cyslight
Plasmid#205077PurposeT7RNAP protein expressionDepositorInsertT7RNAP Cyslight (T7p07 Bacteriophage T7)
UseTagsExpressionBacterialMutationC125S, C347S, C510S, C515S, C530S, C723SPromoterAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
hRad51(A89E)–Cas9(D10A)
Plasmid#125563PurposeExpresses the Rad51(A89E)–Cas9(D10A) fusion construct in mammalian cells under CMV promoterDepositorInsertRad51(A89E)–Cas9(D10A) (RAD51 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-EGFP
Plasmid#114213PurposeAAV vector (control) that targets expression of EGFP to neuronsDepositorInsertEnhanced Green Fluorescent Protein
UseAAV and Mouse TargetingTagsNoneExpressionMammalianMutationNonePromoterhuman synapsin (hSyn)Available sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEHlyA5
Plasmid#168298PurposeSecretion of nanobodies and other polypeptides fused to C-terminal secretion signal of HlyADepositorInsertN‑terminal His tag, VHH, HA and E‑tags, C‑HlyA
UseTagsE-tag, HA-tag, His-tag, Prescission site, and VHH…ExpressionBacterialMutationPromoterLacI-PlacAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
hRad51(S208E-A209D)–Cas9(D10A)
Plasmid#125561PurposeExpresses the hRad51(S208E-A209D)–Cas9(D10A) fusion construct in mammalian cells under CMV promoterDepositorInsertRad51(S208E A209D)–Cas9(D10A) (RAD51 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5FRT/TO_HA-mAID-nanobody
Plasmid#117713PurposeExpression of tetracycline-inducible HA-tagged lysine-less mAID fused to lysine-less vhhGFP4 (mAID-nanobody)DepositorInsertmAID-nanobody
UseTagstriple HAExpressionMammalianMutationcodon optimized mAID for murine expressionPromoterCMVAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
Tailless VinculinTS
Plasmid#26020DepositorInserttailless vinculinTS
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
MAC-tag-C
Plasmid#108077PurposeGateway compatible destination vectors containing StrepIII/HA/BioIDDepositorInsertCCDB (ccdB(letD) )
UseTagsStrepIII-HA-BirA*ExpressionBacterial and MammalianMutationPromoterAvailable sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-StrepHA-SUMO2
Plasmid#66867PurposeMammalian expression of SUMO2 with a strep and an HA tagDepositorInsertSUMO2 (SUMO2 Human)
UseTagsHA and StrepExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-His-hAos1
Plasmid#53135PurposeE.coli expression of full length human Aos1 (SAE1) with Nterminal His-tagDepositorInsertAos1 (SAE1 Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
6xHis(Thrombin)-GFP(TEV)-Drp1-StrepII
Plasmid#174424PurposeExpresses human Drp1 Isoform 3 with EGFP at N-terminus in bacteriaDepositorInsertDrp1
UseTags6xHis(Thrombin)EGFP(TEV) and StrepIIExpressionBacterialMutationNonePromoterT7Available sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
Xenopus APC-myc
Plasmid#16686DepositorInsertAPC (apc.L Frog)
UseXenopus expressionTagsMycExpressionMutationPromoterAvailable sinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only