Addgene

We narrowed to 10,228 results for: T7

Showing: 5001 - 5040 of 10228 results

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1xNLS-pMJ915v2-sfGFP

Plasmid

#88919

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PfICH pET15b

Plasmid

#60684

Purpose

Recombinant protein expression of Isocyanide Hydratase from Pseudomonas fluorescens

Depositor

Mark Wilson

Article

Lakshminarasimhan et al J Biol Chem. 2010 Sep 17;285(38):29651-6

Insert

inhA

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only
TAL3563

Plasmid

#47282

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-stxbp1a-right (stxbp1a Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only
pHYDuet93

Plasmid

#42303

Depositor

Hideo Iwai

Article

Oeemig et al FEBS Lett. 2009 May 6;583(9):1451-6. doi

Insert

NpuDnaEdC6

Use

Tags

H6-GB1

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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2xNLS-pMJ915v2-sfGFP

Plasmid

#88920

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET3CTc3A(1-135)

Plasmid

#63138

Purpose

Expression of human Tc3(1–135) bipartite domain tagged with 6xHis

Depositor

Titia Sixma

Article

Watkins et al Nucleic Acids Res. 2004 Aug 10;32(14):43

Insert

Tc3A

Use

Tags

6xHis

Expression

Bacterial

Mutation

1-135 aa bipartite domain

Promoter

T7

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
TAL3349

Plasmid

#42812

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-Purinergic receptor Y2-right

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 19, 2013

Availability

Academic Institutions and Nonprofits only
pET3a pro-RegIIIg

Plasmid

#64934

Purpose

bacterial expression of full length mouse RegIIIgamma

Depositor

Lora Hooper

Article

Cash et al Protein Expr Purif. 2006 Jul;48(1):151-9

Insert

RegIIIg (Reg3g Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 25, 2015

Availability

Academic Institutions and Nonprofits only
pET15b+PCNA2 L171C

Plasmid

#66177

Purpose

Expresses the L171C mutant of Sulfolobus solfataricus PCNA2

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 2

Use

Tags

His6 tag

Expression

Bacterial

Mutation

L171C

Promoter

T7 promoter

Available since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15b+PCNA1 G108C

Plasmid

#66140

Purpose

Expresses the G108C mutant of Sulfolobus solfataricus PCNA1

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 1

Use

Tags

His6 tag

Expression

Bacterial

Mutation

G108C

Promoter

T7 promoter

Available since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
Stim1-CaRQ

Plasmid

#34560

Depositor

Kevin Truong

Article

Mills et al Chem Biol. 2011 Dec 23;18(12):1611-9.

Insert

Stim1-CaRQ (STIM1 Human)

Use

Tags

YFP

Expression

Bacterial and Mammalian

Mutation

Q63L in RhoA

Promoter

CMV, T7

Available since

March 29, 2012

Availability

Academic Institutions and Nonprofits only
pBluescript-KS-Nrg3-ISH

Plasmid

#87669

Purpose

In situ probe for mouse Nrg3

Depositor

Oscar Marin

Article

Bartolini et al Cell Rep. 2017 Jan 31;18(5):1157-1170. d

Insert

Nrg3 (Nrg3 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7/M13

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
TAL3112

Plasmid

#41286

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-mcu-Left (zgc:153607 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only
TAL3113

Plasmid

#41287

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-mcu-Right (zgc:153607 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only
TAL3233

Plasmid

#42696

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-crispld2-right (crispld2 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2013

Availability

Academic Institutions and Nonprofits only
TAL3232

Plasmid

#42695

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-crispld2-left (crispld2 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only
TAL2281

Plasmid

#36716

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

MYCN-TALEN_Right (Mycn Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2280

Plasmid

#36715

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

MYCN-TALEN_Left (Mycn Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL3339

Plasmid

#42802

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-pink1-right (pink1 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only
TAL3338

Plasmid

#42801

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-pink1-left (pink1 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only
TAL3237

Plasmid

#42700

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-dbx1b-right (dbx1b Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2013

Availability

Academic Institutions and Nonprofits only
TAL3236

Plasmid

#42699

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-dbx1b-left (dbx1b Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only
TAL3180

Plasmid

#41354

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-sox9b-Left (sox9b Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 14, 2013

Availability

Academic Institutions and Nonprofits only
TAL3181

Plasmid

#41355

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-sox9b-Right (sox9b Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only
TAL3224

Plasmid

#42687

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-cebpb-left (cebpb Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only
TAL3225

Plasmid

#42688

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-cebpb-right (cebpb Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only
TAL2405

Plasmid

#36808

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

CDK4-TALEN_Right (Cdk4 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

Sept. 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2404

Plasmid

#36807

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

CDK4-TALEN_Left (Cdk4 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL3202

Plasmid

#41376

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-zbtb7b-Left (zbtb7b Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 7, 2012

Availability

Academic Institutions and Nonprofits only
TAL3203

Plasmid

#41377

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-zbtb7b-Right (zbtb7b Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
TAL3134

Plasmid

#41308

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-parn-Left (parn Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only
TAL3135

Plasmid

#41309

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-parn-Right (parn Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only
TAL3183

Plasmid

#41357

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-spry4-Right (spry4 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only
TAL3182

Plasmid

#41356

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-spry4-Left (spry4 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
pET15b-At 14-3-3 upsilon

Plasmid

#44932

Depositor

Robert Ferl

Article

Wu et al Arch Biochem Biophys. 1997 Mar 1;339(1):

Insert

14-3-3 upsilon (GRF5 Mustard Weed)

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 14, 2013

Availability

Academic Institutions and Nonprofits only
pET15b-At 14-3-3 psi

Plasmid

#44931

Depositor

Robert Ferl

Article

Wu et al Arch Biochem Biophys. 1997 Mar 1;339(1):

Insert

14-3-3 psi (GRF3 Mustard Weed)

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 14, 2013

Availability

Academic Institutions and Nonprofits only
TAL3194

Plasmid

#41368

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-tfec-Left (tfec Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 7, 2012

Availability

Academic Institutions and Nonprofits only
TAL3195

Plasmid

#41369

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-tfec-Right (tfec Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 7, 2012

Availability

Academic Institutions and Nonprofits only
TAL3129

Plasmid

#41303

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-nphs2-Right (nphs2 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
TAL3186

Plasmid

#41360

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-svila-Left (svila Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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