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NPM1_mNeonGreen_Donor

Plasmid

#178093

Purpose

HDR donor plasmid to tag NPM1 with mNeonGreen using eSpCas9(1.1)_No_FLAG_NPM1_G6.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

NPM1-mNeonGreen HDR donor

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-Brainbow3.2-PGK-PuroTK

Plasmid

#159928

Purpose

Brainbow transgenesis

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Stem Cell Res. 2021 Jan 8;51:102164. doi

Inserts

NFPnls
mOrange2
EGFP
mKate2

Expression

Mammalian

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pRCC-N

Plasmid

#81192

Purpose

Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance

Depositor

Eckhard Boles

Article

Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

Inserts

Cas9
empty gRNA cassette

Use

CRISPR

Tags

NTS

Expression

Yeast

Mutation

Please see depositor comments below. and WT - cod…

Promoter

ROX3 and SNP52p

Available Since

Sept. 8, 2016

Availability

Academic Institutions and Nonprofits only

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piggyBac CMV-GFP-FRT

Plasmid

#92078

Purpose

production of transgenic animal and recombination using flipase

Depositor

Jae Yong Han

Article

Lee et al FASEB J. 2016 Feb;30(2):555-63. doi: 10.

Insert

CMV-eGFP and TK-Neo between FRT3 and FRT

Use

Piggybac

Expression

Mammalian

Promoter

CMV

Available Since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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pY2

Plasmid

#218115

Purpose

Activity reporter backbone with an empty protease cassette (BsaI) under (lexA-box)3PminCYC1 promoter and an empty substrate cassette (BsmBI) under pGAL1 promoter.

Depositor

Carl Denard

Article

Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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Prab-3::NLS::GCaMP6s

Plasmid

#68119

Purpose

Nuclear Localized Fluorescent Calcium Indicator for pan-neuronal expression in C. Elegans

Depositor

Andrew Leifer

Article

Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

Insert

rab-3::NLS::GCaMP6s

Expression

Worm

Promoter

rab-3

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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AAVTRE3_smFP_Myc

Plasmid

#201208

Purpose

AAV vector for TRE(TRE3G)-driven smFP_Myc (Myc-tagged spaghetti monster) expression

Depositor

Tetsuo Yamamori

Article

Watakabe et al J Vis Exp. 2025 Jan 17;(215). doi: 10.37

Insert

smFP _Myc (from addgene #59757)

Use

AAV

Expression

Bacterial and Mammalian

Promoter

TRE3G

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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p15a_AcCas12n

Plasmid

#203806

Purpose

p15a-based plasmid containing a rpsL driven AcCas12n cassette and the araBAD-driven lambda-red recombinase cassette. Apramycin resistance.

Depositor

Quanjiang Ji

Article

Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

Insert

AcCas12n

Use

CRISPR

Promoter

rpsl

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pES134

Plasmid

#127551

Purpose

Variant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F3-aadA-F3 cassette orthogonal to FRT-KanR-FRT in pKD13

Depositor

Emmanuele Severi, Gavin Thomas

Article

Wu et al Microbiology (Reading). 2024 Mar;170(3).

Insert

F3-flanked spectinomycin resistance cassette

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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T2A 3xNLS GFP

Plasmid

#227721

Purpose

Plasmid contains T2A 3xNLS GFP, with some nlp-51 homology sequence before, Amp Resistant

Depositor

Oliver Hobert, Haosheng Sun

Article

Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

Insert

T2A 3xNLS GFP with some nlp-51 homology sequence (nlp-51 Nematode)

Use

Cloning vector

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pBWB424

Plasmid

#140638

Purpose

RSF1010-Cas9-vanAB gRNA. Vector with constitutive Cas9 and gRNA targeting vanAB locus.

Depositor

Keith Tyo

Article

Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

Insert

Cas9

Expression

Bacterial

Available Since

May 11, 2020

Availability

Academic Institutions and Nonprofits only

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pNeuroD-ires-GFP

Plasmid

#61403

Purpose

Mammalian expression vector under the neuron-specific promoter NeuroD. Co-expression of GFP with IRES sequence

Depositor

Franck Polleux

Article

Guerrier et al Cell. 2009 Sep 4;138(5):990-1004. doi: 1

Type

Empty backbone

Expression

Mammalian

Promoter

NeuroD

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pBWB419

Plasmid

#140637

Purpose

RSF1010-Cas9-pcaHG gRNA. Vector with constitutive Cas9 with gRNA targeting pcaHG locus.

Depositor

Keith Tyo

Article

Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

Insert

Cas9

Expression

Bacterial

Available Since

May 11, 2020

Availability

Academic Institutions and Nonprofits only

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pDM1081

Plasmid

#108981

Purpose

Knockout cassette Hygromycin

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Insert

Knockout cassette Hygromycin

Use

Cre/Lox; Dictyostelium discoideum knockout vector

Promoter

coaA

Available Since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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pDD283

Plasmid

#66824

Purpose

YPET^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Bob Goldstein

Article

Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

Insert

YPET-C1^SEC^3xFlag

Use

CRISPR and Cre/Lox

Tags

3xFlag and C. elegans codon-optimized YPET

Expression

Worm

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pCRISPomyces-1

Plasmid

#61736

Purpose

Streptomyces expression of codon-optimized Cas9, tracrRNA, and custom crRNA

Depositor

Huimin Zhao

Article

Cobb et al ACS Synth Biol. 2014 Dec 8.

Inserts

sSpCas9
tracrRNA
crRNA cassette

Use

CRISPR

Expression

Bacterial

Mutation

BbsI-flanked lacZ cassette inserted in place of s…

Promoter

gapdhp(EL), rpsLp(CF), and rpsLp(XC)-BbsI

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pLX317 GFP

Plasmid

#193681

Purpose

Constitutive lentiviral expression of GFP

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

GFP

Use

Lentiviral

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pNM3689

Plasmid

#154111

Purpose

miniMos single component RMCE landing site vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

loxP::SEC::loxP::mex-5p::FLP::sl2::nNeonGreen::glh-2 3'::rpl-28p::FRT::GFP::his-58::unc-54 3'::FRT3

Use

Minimos

Promoter

not applicable

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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M-NylC_pET28a

Plasmid

#215409

Purpose

Expression construct for M-NylC gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

M-NylC

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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NylCK_pET28a

Plasmid

#215422

Purpose

Expression construct for NylCK gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylCK

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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