178,119 results
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pEGFP-SF2
Plasmid#17990DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSF2 (SRSF1 Human)
TagsEGFPExpressionMammalianMutationcontains SF2 (aa1-210)Available sinceMay 13, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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pCS2FLAG
Plasmid#16331DepositorInsertFlag tag
UseVertebrate expressionTagsFlagMutationFlag tag inserted into pCS2+ polylinkerAvailable sinceDec. 21, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBabe-puro largeTcDNA
Plasmid#14088DepositorPublicationAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRSET Nucleolin
Plasmid#13037DepositorAvailable sinceOct. 5, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-10His-mStayGold (E138D)
Plasmid#211363PurposeMammalian expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Contains a N-terminal 10xHis tag.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmStayGold
Tags10xHis-tagExpressionMammalianMutationE138DPromoterCMVAvailable sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCSII-EF/mt-(n1)StayGold
Plasmid#185823PurposeA soluble photostable fluorescent marker of the mitochondrial matrix. The StayGold gene has codon optimization for mammalian expression.DepositorInsertmt-(n1)StayGold
UseLentiviralExpressionMammalianPromoterEF1aAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-T7-SpRY-P2A-EGFP (RTW4830)
Plasmid#139989PurposeCMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…PromoterCMV and T7Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRDA_052
Plasmid#136474PurposeLentiviral expression of AsCas12a gRNADepositorInsertPuromycin resistance
UseLentiviral and Synthetic BiologyPromoterEF1aAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHelper_ShCAST_sgRNA
Plasmid#127921PurposeExpresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)
Available sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKLV2-EF1a-Cas9Bsd-W
Plasmid#68343PurposeLentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the C-terminusDepositorInsertEF1a-Cas9 cassette, WPRE
UseCRISPR and LentiviralTagsCas9 fused with Flag-tag and a NLS at the N termi…PromoterEF1aAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUC18R6KT-mini-Tn7T-Km
Plasmid#64969Purposemini-Tn7 base vector with transcriptional terminator and oriTDepositorPublicationTypeEmpty backboneExpressionBacterialAvailable sinceNov. 2, 2015AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pFLAG-YAP1
Plasmid#66853PurposeExpress FLAG-tagged YAP1 in mammalian cellsDepositorAvailable sinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
phage UBC NLS-HA-2XMCP-tagRFPt
Plasmid#64541PurposeUsed to label reporter mRNAs. Lentiviral expression of the MCP protein fused to tagRFP.DepositorInsertMCP
UseLentiviralTagsHA, NLS, and tagRFP-TExpressionMammalianPromoterhuman ubiquitin C promoterAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-ChrimsonR-tdT
Plasmid#59169PurposeExpression vector for ChrimsonR-tdTomatoDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertChrimsonR-tdTomato
TagstdTomatoExpressionMammalianMutationK176RPromoterCAGAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-DIO-rM3D(Gs)-mCherry
Plasmid#50458PurposeDouble floxed Gs-coupled rM3D DREADD fused with mCherry under the control of human synapsin promoterDepositorHas serviceAAV8 and AAV RetrogradeInsertrM3D-mCherry
UseAAVTagsHA and mCherryMutationSee supplemental documents for DREADD mutationsPromoterhuman Synapsin 1Available sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pICH86966::AtU6p::sgRNA_PDS
Plasmid#46966PurposeExpresses an sgRNA targeting the PDS gene in Nicotiana benthamiana from the Arabidopsis U6 promoterDepositorInsertAtU6p::sgRNA_PDS
UseCRISPR; Plant expressionAvailable sinceAug. 13, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP VAMP2
Plasmid#42308DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertVAMP 2
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceMarch 12, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCI-ASC-HA
Plasmid#41553DepositorInsertASC (PYCARD Human)
TagsHAExpressionMammalianPromoterCMV immediate-early enhancer/promoterAvailable sinceDec. 20, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by
