165,947 results

1. pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G

   Plasmid

   #167935

   Purpose

   Inducible CRISPRi expression

   Depositor

   Emma Rawlins

   Article

   Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

   Insert

   KRAB-dCas9-DHFR
   

   Use

   Lentiviral

   Promoter

   tetON

   Available Since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMJ915

   Plasmid

   #69090

   Purpose

   Express Streptococcus pyogenes Cas9 carrying two C-terminal SV40 NLS

   Depositor

   Jennifer Doudna

   Article

   Lin et al Elife. 2014 Dec 15;3:e04766. doi: 10.755

   Insert

   cas9
   

   Tags

   MBP, hexa His, and two SV40 NLS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE-EGFR

   Plasmid

   #116731

   Purpose

   Lentiviral expression of EGFR

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   EGFR (EGFR Human)
   

   Use

   Lentiviral

   Mutation

   WT

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pvPE-V4

   Plasmid

   #240288

   Purpose

   Encodes fourth generation porcine endogenous retrovirus-derived prime editing (pvPE) system into mammalian cells for gene editing.

   Depositor

   Lusheng Huang

   Article

   Liu et al Trends Biotechnol. 2025 Aug 30:S0167-779

   Insert

   pvPE-V4
   

   Use

   CRISPR

   Tags

   SV40 NLS and c-Myc NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV4-ApoE3

   Plasmid

   #87086

   Purpose

   Expresses ApoE3 in Mammalian cells

   Depositor

   Bradley Hyman

   Article

   Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

   Insert

   Apolipoprotein E3 (APOE Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR-rtTA3G

   Plasmid

   #139556

   Purpose

   rtTA ORF with stop codon between attL1-attL2 on Gateway entry vector

   Depositor

   Minoru Ko

   Insert

   rtTA3G
   

   Expression

   Mammalian

   Available Since

   March 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. TRPML1-HA

   Plasmid

   #18825

   Depositor

   Craig Montell

   Article

   Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

   Insert

   TRPML1 (MCOLN1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hsyn-GRAB_HA1h

   Plasmid

   #190473

   Purpose

   Express the high-affinity histamine sensor GRAB_HA1h in neurons

   Depositor

   Yulong Li

   Article

   Dong et al Neuron. 2023 Mar 7:S0896-6273(23)00128-9

   Insert

   Histamine sensor GRAB_HA1h (high affinity)
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pdCas9-DNMT3A-EGFP

   Plasmid

   #71666

   Purpose

   Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA

   Depositor

   Vlatka Zoldos

   Article

   Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

   Insert

   S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A S. pyogenes, Synthetic, Human)
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9

   Promoter

   CBh

   Available Since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX-FFSS-Cyclin D1

    Plasmid

    #215151

    Purpose

    Expresses FFSS-Cyclin D1 in mammalian cells from a lentiviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Lentiviral

    Tags

    FFSS (FLAG-FLAG-STREP-STREP)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. htrpC6-YFP

    Plasmid

    #21084

    Depositor

    Craig Montell

    Article

    Kwon et al Mol Cell. 2007 Feb 23. 25(4):491-503.

    Insert

    TrpC6 (TRPC6 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR223-BMPR2

    Plasmid

    #23669

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    BMPR2 (BMPR2 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pDisCoTune

    Plasmid

    #173713

    Purpose

    Promotes expression of disulfide-rich protein in E. coli T7 expression system. The plasmid encodes expression of the folding factors Erv1p and hPDI controlled by the Ptac promoter.

    Depositor

    Morten Norholm

    Article

    Bertelsen et al Microb Biotechnol. 2021 Nov;14(6):2566-2

    Inserts

    ERV1 (ERV1 Budding Yeast)
    PDI
    

    Expression

    Bacterial

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCW empty vector

    Plasmid

    #184708

    Purpose

    Lentiviral empty vector for doxycycline-inducible expression

    Depositor

    Alessia Ciarrocchi, Gloria Manzotti

    Article

    Gugnoni et al J Exp Clin Cancer Res. 2022 Mar 25;41(1)

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. FgH1tUTG

    Plasmid

    #70183

    Purpose

    Inducible expression of guide RNA with fluorescent GFP reporter

    Depositor

    Marco Herold

    Article

    Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

    Insert

    H1-Tet-sgrna cassette
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)

    Plasmid

    #139999

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

    Promoter

    CAG

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMarC9-R6k

    Plasmid

    #89477

    Purpose

    transposon plasmid, compatible with Tn-Seq

    Depositor

    George Church

    Article

    Lee et al Nat Microbiol. 2019 Apr 8. pii: 10.1038/

    Insert

    pMarC9-R6k
    

    Available Since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. rtTA-N144

    Plasmid

    #66810

    Purpose

    2nd generation lentiviral transfer plasmid. Expresses the reverse tetracycline-controlled transactivator (rtTA) with hygromycin resistance

    Depositor

    Andrew Yoo

    Article

    Richner et al Nat Protoc. 2015 Oct;10(10):1543-55. doi

    Insert

    Reverse tetracycline-controlled transactivator
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    Human EF1a

    Available Since

    Sept. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. MSCV-C 18E6

    Plasmid

    #37884

    Depositor

    Karl Munger

    Article

    Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

    Insert

    HPV 18E6 (E6 HPV)
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Promoter

    PKG

    Available Since

    July 26, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pAAV-hSyn-FLEX-TeLC-P2A-dTomato

    Plasmid

    #159102

    Purpose

    Cre-dependent tetanus toxin light chain construct with nuclear-localized dTomato fluorophore for conditional silencing of neurons.

    Depositor

    Sandeep Datta

    Article

    Pashkovski et al Nature. 2020 Jul;583(7815):253-258. doi:

    Insert

    TeLC-P2A-NLS-dTomato
    

    Use

    AAV

    Promoter

    hSynapsin

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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