170,806 results
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Plasmid#223747PurposeExpression of recombinant protein for purificationDepositorInsertLambda-PPase
Tags6xHis-TEVExpressionBacterialAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
UAS:mCherry
Plasmid#170994PurposeUAS:GAL4 repeat-containing promoter drives simple mCherry reporter gene. Blasticidin selectable.DepositorInsertUAS:mCherry
UseLentiviral and Synthetic BiologyExpressionMammalianPromoterSynthetic 14x UAS:Gal4Available SinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
EZ-Tet-pLKO-Blast
Plasmid#85973PurposeTet/Dox inducible shRNA lentivirus with blasticidin selectionDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral and RNAiPromoterH1 / TetOAvailable SinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pHCMV-AmphoEnv
Plasmid#15799DepositorInsert4070A Amphotropic Murine leukemia virus envelope gene
ExpressionMammalianMutationN/AAvailable SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
TLCV2-DelGFP
Plasmid#133302PurposeEGFP reporter was removed in the TLCV2 vector so it could be used for inducible gene CRISPR knockout in cell lines with an eGFP reporter.DepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT31
Plasmid#223403PurposeT-DNA vector for SpCas9-D10A based A-to-G base editing for dicot plants; NGG PAM; SpCas9D10A-ABE was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter; Hygromycin for plants selection.DepositorInsertAtUBQ10-ecTadA8e-SpCas9-D10A-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-SOUL-P2A-TdTomato-WPRE-hGH-polyA
Plasmid#177576PurposeExpresses SOUL in neuronal cell typesDepositorInsertSOUL
UseAAVTagstdTomatoPromoterhSynAvailable SinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1-hVAP-B
Plasmid#104448PurposeExpression of human VAP-B fused to GFP in mammalian cellsDepositorInsertvesicle-associated membrane protein-associated protein B (VAPB Human)
TagsEGFP and HAExpressionMammalianAvailable SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSETB/mStayGold
Plasmid#212017PurposemStayGold has an n1 adaptor at its N-terminus and is useful for C-terminal tagging. This construct is used also for expression of mStayGold itself.DepositorInsertmStayGold
ExpressionBacterialPromoterT7Available SinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJR104
Plasmid#187243PurposeLentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expressionDepositorInsertLentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression
UseLentiviralAvailable SinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro
Plasmid#71236PurposeExpress sgRNA and dCas9-KRAB from lentiviral vectorDepositorInsertshumanized dCas9-KRAB T2A Puro
sgRNA
UseCRISPR and LentiviralTagsFlagMutationD10A and H840AAvailable SinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCherry-DFCP1
Plasmid#86746PurposeN-terminal mCherry-tagged protein expression in mammalian cellsDepositorAvailable SinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
3` CC: Hygro – GFP-N – loxP – mScarlet
Plasmid#219564Purpose3` circularization cassette.To induce Cre-mediated circularization or inversion of a genomic region with concomitant GFP reconstitution and mScarlet expression from the chromosomeDepositorInsert3` CC: Hygro – GFP-N – loxP – mScarlet
UseUnspecifiedPromoterEF1-aAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pscALPS- SIV-MAC251 vpx
Plasmid#115810PurposeEncodes codon optimized 3xFLAG-VpxMAC251 and puromycin resistance proteinDepositorInsertSIVMAC251 Vpx
UseLentiviralTags3x-FLAGPromoterSFFVAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLM494
Plasmid#100539PurposeCEN/ARS plasmid harboring expression cassettes for crtI, crtYB, crtE and tHMG1 of the β-carotene pathway, each flanked by loxPsym sites.DepositorInsertsGGPP synthase
phytoene desaturase
phytoene-beta carotene synthase
HMG CoA reductase
UseCre/Lox and Synthetic BiologyExpressionYeastPromoterPGK1, TDH3, TIP1, and ZEO1Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAD-GamS
Plasmid#99246PurposeExpresses 6xHis-tagged GamS from T7lac promoter in the E.coli Tuner (DE3) strain. See notes below for important strain information.DepositorInsertgamS (with C-terminal 6xHis tag)
Tags6xHis tagExpressionBacterialPromoterT7lacAvailable SinceOct. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV rtTA3 Hygro (w785-1)
Plasmid#26730Purpose3rd gen lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, HygroDepositorInsertTetracycline repressor A3 mutant
UseLentiviralExpressionMammalianAvailable SinceJune 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
DLX2-hygro
Plasmid#97330PurposeGenerating lentiviruses to express Dlx2 and hygromycin resistanceDepositorAvailable SinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-EF1a-Zim3-dCas9-P2A-GFP
Plasmid#188778PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionPromoterEF1aAvailable SinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only