Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 5,287 results for: pcdna3.1

Showing: 5041 - 5080 of 5287 results

  1. SpyTag-Quartet

    Plasmid
    #214727
    Purpose
    In mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses SHC014, Rs4081, RaTG13, and SARS-CoV-2 each separated by GS-linkers with an N-terminal SpyTag fusion
    Depositor
    Mark Howarth
    Article
    Hills et al Nat Nanotechnol. 2024 May 6. doi: 10.103
    Insert
    SpyTag-Quartet (S Synthetic)
    Use
    Tags
    SpyTag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    214727-plasmid-map-sequence-id-435532

    Enlarge

  2. SpyTag-Alternate Quartet

    Plasmid
    #214728
    Purpose
    In mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses pang17, RmYN02, Rf1, and WIV1 each separated by GS-linkers with an N-terminal SpyTag fusion
    Depositor
    Mark Howarth
    Article
    Hills et al Nat Nanotechnol. 2024 May 6. doi: 10.103
    Insert
    SpyTag-Alternate Quartet
    Use
    Tags
    SpyTag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    214728-plasmid-map-sequence-id-435533

    Enlarge

  3. SpyTag-Quartet [SARS1]

    Plasmid
    #214729
    Purpose
    In mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses SHC014, Rs4081, RaTG13, and SARS-CoV each separated by GS-linkers with an N-terminal SpyTag fusion
    Depositor
    Mark Howarth
    Article
    Hills et al Nat Nanotechnol. 2024 May 6. doi: 10.103
    Insert
    SpyTag-Quartet [SARS1] (S Synthetic)
    Use
    Tags
    SpyTag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    214729-plasmid-map-sequence-id-435534

    Enlarge

  4. SpyTag-Kraken Quartet

    Plasmid
    #214730
    Purpose
    In mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses SHC014, Rs4081, RaTG13, and SARS-CoV-2 XBB.1.5 separated by GS-linkers with an N-terminal SpyTag
    Depositor
    Mark Howarth
    Article
    Hills et al Nat Nanotechnol. 2024 May 6. doi: 10.103
    Insert
    SpyTag-Kraken Quartet (S Synthetic)
    Use
    Tags
    SpyTag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    214730-plasmid-map-sequence-id-435535

    Enlarge

  5. SpyTag-Quartet_NoLinker

    Plasmid
    #214731
    Purpose
    In mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses SHC014, Rs4081, RaTG13, and SARS-CoV-2 with no linkers between the RBDs and an N-terminal SpyTag
    Depositor
    Mark Howarth
    Article
    Hills et al Nat Nanotechnol. 2024 May 6. doi: 10.103
    Insert
    SpyTag-Quartet_NoLinker (S Synthetic)
    Use
    Tags
    SpyTag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    214731-plasmid-map-sequence-id-435536

    Enlarge

  7. pCDNA3-SREBP-1 S1P and S2P mutations (U124AFG200-5)

    Plasmid
    #210767
    Purpose
    Mammalian expression of SREBP-1 (isoform 1) with mutations in residues reported to be critical for S1P and S2P cleavage.
    Depositor
    Allan Weissman
    Article
    Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.
    Insert
    SREBP-1 (SREBF1 Human)
    Use
    Tags
    E-tag and T7x2
    Expression
    Mammalian
    Mutation
    DRSR 514-517 to AS; 557 R to A; multiple silent m…
    Promoter
    Available since
    April 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    210767-plasmid-map-sequence-id-421642

    Enlarge

  15. anti-GFAP R416WT scFv [N206B/9] with sortase tag

    Plasmid
    #204420
    Purpose
    Mammalian Expression of anti-GFAP scFv with a sortase tag for direct dye conjugation. Derived from hybridoma N206B/9.
    Depositor
    Jeff Lichtman
    Article
    Han et al bioRxiv. 2023 Jun 8:2023.05.20.540091. d
    Insert
    Recombinant mouse scFv targeting GFAP R416WT (Homo sapiens)
    Use
    Tags
    6xHis tag and Sortase tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204420-plasmid-map-sequence-id-406611

    Enlarge

  31. TRUPATH Triple Gaq

    Plasmid
    #196058
    Purpose
    Encodes a G alpha subunit (GNAQ) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaQ-RLuc8 (GNAQ Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196058-plasmid-map-sequence-id-385868

    Enlarge

  33. TRUPATH Triple Gai1

    Plasmid
    #196048
    Purpose
    Encodes a G alpha subunit (GNAl1) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphai1-RLuc8 (GNAI1 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196048-plasmid-map-sequence-id-385883

    Enlarge

  34. TRUPATH Triple GaoA

    Plasmid
    #196051
    Purpose
    Encodes a G alpha subunit (GNAO1 Isoform Alpha 1) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaoA-RLuc8
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196051-plasmid-map-sequence-id-385865

    Enlarge

  35. TRUPATH Triple Ga13

    Plasmid
    #196062
    Purpose
    Encodes a G alpha subunit (GNA13) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlpha13-RLuc8 (GNA13 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196062-plasmid-map-sequence-id-385881

    Enlarge

  36. TRUPATH Triple GaoB

    Plasmid
    #196052
    Purpose
    Encodes a G alpha subunit (GNAO1 Isoform Alpha 2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaoB-RLuc8
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196052-plasmid-map-sequence-id-387470

    Enlarge

  37. pcDNA-dCas9-p300 Core

    Plasmid
    #61357
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, S. pyogenes, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61357-plasmid-map-sequence-id-358013

    Enlarge

  38. pcDNA-dCas9-p300 Core (D1399Y)

    Plasmid
    #61358
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, S. pyogenes, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; D1399Y mutation i…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61358-plasmid-map-sequence-id-99789

    Enlarge

  40. lucMAPT-GenRep

    Plasmid
    #214674
    Purpose
    MAPT Exon 10 reporter, with Exon 10 and 100bp flanking intronic regions replaced with a BamHI/EcoRI cloning site for testing different exons
    Depositor
    Eugene Yeo
    Article
    Schmok et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038
    Insert
    MAPT (MAPT Human)
    Use
    Tags
    Firefly Luciferase, Renilla Luciferase, and V5
    Expression
    Mammalian
    Mutation
    Contains Exon 9 and Exon 11, with a BamHI EcoRI c…
    Promoter
    CMV
    Available since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214674-plasmid-map-sequence-id-424937

    Enlarge

Showing: 5041 - 5080 of 5287 results