We narrowed to 15,043 results for: NOL
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Plasmid#228145PurposePlasmid contains the secretion signal peptide sequence of the yeast MEL1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).DepositorInsertsp_P04824_MEL1_YEASX:0-18
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
SP_A2
Plasmid#228146PurposePlasmid contains the secretion signal peptide sequence of the yeast (S. uvarum) SPR1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).DepositorInsertsp_Q876J2_SPR1_SACU7:0-20
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
SP_A3
Plasmid#228147PurposePlasmid contains the secretion signal peptide sequence of the human ECM33 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).DepositorInsertsp_P38248_ECM33_YEAST:0-19
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
SP_A4
Plasmid#228148PurposePlasmid contains the secretion signal peptide sequence of the human CHADL gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).DepositorInsertsp_Q6NUI6_CHADL_HUMAN:0-30
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
SP_A5
Plasmid#228149PurposePlasmid contains the secretion signal peptide sequence of the human GDF8 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).DepositorInsertsp_O14793_GDF8_HUMAN:0-18
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRRL-SFFV_V5-CHIC2_IFRPDdel_Tomato
Plasmid#238126PurposeExpresses V5-tagged CHIC2 (with IFRPD deletion) with Tomato selectable marker.DepositorArticleInsertV5-tagged CHIC2 with IFRPD deletion
UseTagsV5ExpressionMammalianMutationDeleted amino acids 160-165 (IFRPD).PromoterSFFVAvailable sinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRRL-SFFV_V5-CHIC2-K117R_Tomato
Plasmid#238127PurposeExpresses V5-tagged CHIC2 (with K117R mutation) with Tomato selectable marker.DepositorArticleInsertV5-tagged CHIC2 with K117R mutation
UseTagsV5ExpressionMammalianMutationMutated Lysine 117 to Arginine.PromoterSFFVAvailable sinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRRL-SFFV_STUB1-UBOXdel-V5_Tomato
Plasmid#238129PurposeExpresses V5-tagged STUB1 (with UBOX deletion) with Tomato selectable marker.DepositorArticleInsertV5-tagged-STUB1
UseTagsV5ExpressionMammalianMutationDeleted UBOX domain (amino acids 224-294)PromoterSFFVAvailable sinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLX_305_OVA_Blasticidin
Plasmid#238124PurposeEnables expression of Ovalbumin with Blasticidin selectable marker.DepositorArticleInsertOvalbumin (OVAL Chicken)
UseTagsExpressionMammalianMutationPromoterHuman PGKAvailable sinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXYB162
Plasmid#239633PurposeRibozyme split sfGFP linked with GFPuv via T2A expressionDepositorInsertsfGFPn_Y66-Ribozyme-2nd_sfGFPc-T2A-GFPuv
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceJune 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXYB163
Plasmid#239634PurposeDeactivated ribozyme split sfGFP linked with GFPuv via T2A expressionDepositorInsertsfGFPn_Y66-dRibozyme-2nd_sfGFPc-T2A-GFPuv
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceJune 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1-SARS2-Spike-HiBiT
Plasmid#191212PurposeTransient expression in mammalian cells of SARS-CoV-2 (A1) spike protein tagged on the C-terminus with HiBiT, the high affinity small fragment of split nanoluciferase assayDepositorInsertSARS-CoV-2 spike_WT (S SARS CoV-2)
UseTagsHiBiT (high affinity small nanoluciferase fragmen…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLUEF1 IRES GFP (backbone)
Plasmid#224466PurposeEf1a downstream of a ubiquitous chromatin opening element drives expression of GOI and IRES GFPDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterEf1aAvailable sinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGY18
Plasmid#221708PurposeEmpty Cas9 vector for multiplex gene editing in fungiDepositorTypeEmpty backboneUseCRISPR; AspergillusTagsExpressionBacterialMutationPromoterAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-His-E1GFP
Plasmid#233885PurposeBacterial Expression of recombinant His-tagged E1GFPDepositorInsertE1GFP
UseTagsHisExpressionBacterialMutationT65S and T203Y compared to EGFPPromoterT7 promoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4TO_24xGCN4_PCC_24xPP7
Plasmid#236094PurposeConstitutively encodes 24xGCN4-PCC-24xPP7 stem loops, for visualization of the Pcc mRNA.DepositorInsert24xGCN4-PCC-24xPP7
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4TO_24xGCN4_MUT_24xPP7
Plasmid#236093PurposeConstitutively encodes 24xGCN4-MUT-24xPP7 stem loops, for visualization of the Mut mRNA.DepositorInsert24xGCN4-MUT-24xPP7
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN_GFP
Plasmid#235611PurposeEncodes GFP. For use in transfections as a negative control without epitope tags.DepositorInsertGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN_NES_V5_GFP
Plasmid#235632PurposeEncodes V5-GFP with an N-terminal nuclear export sequence. Used as a no-ALIBi negative control.DepositorInsertGFP
UseTagsNES-V5ExpressionMammalianMutationPromoterAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80ENSF9-Pct3-(–)-Eldh2(cHis6)-Accs(ElA2)
Plasmid#233868PurposeExpresses His-tagged Eggerthella lenta A2 (–)-Eldh2 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purificationDepositorInsert(–)-Eldh2
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pISEre1reRNA
Plasmid#226821PurposeExpressing ISEre1 guide RNA targeting maeB site controled by PJ23119DepositorInsertISEre1 none target guide RNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80EN16-HcdhA(cHis8)BC-partial cluster(ElA2)
Plasmid#225328PurposeExpresses Eggerthella lenta A2 Hcdh partial gene cluster (inactive) in Gordonibacter urolithinfaciens DSM 27213DepositorInsertHcdh partial gene cluster
UseTagsHis8ExpressionBacterialMutationPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
GB4003
Plasmid#215248PurposeModule for the constitutive expression of PIF6 and PhyB:VP16DepositorInsertPIF6-PhyB:VP16
UseSynthetic BiologyTagsExpressionMutationV234L in PIF6- please see dep. sequence, BsaI and…PromoterAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80EN6-Elen0497-ElVadh-noAccs(ElDSM2243)
Plasmid#225314PurposeExpresses Eggerthella lenta DSM 2243 El Vadh without accessory genes (inactive) in Gordonibacter urolithinfaciens DSM 27213DepositorInsertEl Vadh (Elen_0497 Eggerthella lenta)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD70EN20-Pct5-GPA_25810-(+)-Eldh1(GpDSM19378)
Plasmid#225306PurposeExpresses Gordonibacter pamelaeae DSM 19378 (+)-Eldh1 in Eggerthella lenta DSM 2243 with a cumate inducible promoterDepositorInsert(+)-Eldh1
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD70EN21-Pct5-GPA_25800-(+)-Eldh2(GpDSM19378)
Plasmid#225307PurposeExpresses Gordonibacter pamelaeae DSM 19378 (+)-Eldh2 in Eggerthella lenta DSM 2243 with a cumate inducible promoterDepositorInsert(+)-Eldh2
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80ENSF25-Pct3-(–)-Eddh(nHis6)(G3C)
Plasmid#233873PurposeExpresses His-tagged Gordonibacter pamelaeae 3C (–)-Eddh with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purificationDepositorInsert(–)-Eddh
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80ENSF30-DadhABC(cHis8)RS-Accs(Cadh)(ElA2)
Plasmid#233876PurposeExpresses Eggerthella lenta A2 Dadh with the accessory genes from the cadh gene cluster in Gordonibacter urolithinfaciens DSM 27213DepositorInsertDadh
UseTagsHis8ExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMK83
Plasmid#226793PurposeIntegrates slr0214 (HIP1 methyltransferase) into gidB-atpI locus of E. coli DH10BDepositorInsertslr0214 (slr0214 Synechocystis sp. PCC 6803)
UseTagsExpressionBacterialMutationPromoterTetracycline resistance cassette promoter (consti…Available sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
GB4653
Plasmid#215267PurposeTU for the constitutive expression of the flippase (FLP) recombinase with a nopaline synthase (Nos) promoterDepositorInsertpNos:FLP:tNos
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
GB4829
Plasmid#215275PurposeTU for the constitutive expression of the N. nambi NPGA gene under the P35S promoter and the TNOS terminatorDepositorInsertP35S:NPGA:TNOS
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEL1022
Plasmid#225593PurposeThe backbone vector for rice genome editing is based on HkCas12aDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEL1023
Plasmid#225594PurposeThe backbone vector for rice genome editing is based on PrCas12aDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEL1024
Plasmid#225595PurposeThe Dual Pol II backbone vector for rice genome editing is based on Mb3Cas12aDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEL1025
Plasmid#225596PurposeThe STU backbone vector for rice genome editing is based on Mb3Cas12aDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEL1026
Plasmid#225597PurposeThe STU backbone vector for rice genome editing is based on Mb3Cas12a-R (D172R)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
Sc++
Plasmid#198545PurposeExpresses human codon-optimized Sc++ and blasticidin resistance: EFS promoter-Sc++-NLS-FLAG-P2A-BSDDepositorInsertSc++
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpG
Plasmid#198543PurposeExpresses human codon-optimized SpG and blasticidin resistance: EFS promoter-SpG-NLS-FLAG-P2A-BSDDepositorInsertSpG
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEM_ΔndhD2.km
Plasmid#185532Purposea pGEM-T easy vector containing the kanamycin resistance cassette flanked by the two regions for the double homologous recombination on the ndhD2 locusDepositorInsertnptII gene flanked by ndhD2 upstream and downstream regions
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEM_Δflv3.sm
Plasmid#185533Purposea pGEM-T easy vector containing the streptomycin/spectinomycin resistance cassette flanked by the two regions for the double homologous recombination on the flv3 locusDepositorInsertaadA gene flanked by flv3 upstream and downstream regions
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYAMTr2GCsgSeLEU2
Plasmid#224870PurposeDisruption of S. eubayanus type LEU2 genesDepositorInsertpYAMTr2GC having the guide sequence SeLEU2 (DI49_0736 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYAMTrGCsgScLEU2
Plasmid#224869PurposeDisruption of S. cerevisiae type LEU2 genesDepositorInsertpYAMTrGCas having the guide sequence ScLEU2 (LEU2 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYAMTr2GCsgScLEU2
Plasmid#224868PurposeDisruption of S. cerevisiae type LEU2 geneDepositorInsertpYAMTr2GC having the guide sequence ScLEU2 (LEU2 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYFAC-ubi-Y-pyrG
Plasmid#184497PurposeAMA1 based vector for optimised episomal expression in filamentous fungi, with increased selection stringency using the modified ubi-Y-pyrG marker (high stringency).DepositorTypeEmpty backboneUseSynthetic Biology; Expression in aspergillus and …TagsExpressionMutationPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB3774
Plasmid#215247PurposeCDS of CPH from N. nambi codon optimized for N.benthamiana. This gene is the final step and recycling of the bioluminiscente pathwayDepositorInsertCPH
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB4807
Plasmid#215273PurposeModule for the expressino of the N. nambi HISPS gene under the PNOS promoter and the constitutive expression of EGFP and p19.DepositorInsertPNOS:HISPS-SF-EGFP-p19
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB4808
Plasmid#215274PurposeModule for the expressino of the N. nambi HISPS gene under the P35S promoter and the constitutive expression of EGFP and p19.DepositorInsertP35S:HISPS-SF-EGFP-p19
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQA-LP580
Plasmid#222959PurposeExpresses R141S K142S LoaP double mutantDepositorInsertantiterminator protein LoaP
UseTags10xHIS-SUMOExpressionBacterialMutationmutated Arginine 141 to Serine and Lysine 142 to …PromoterT7Available sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQA-LP579
Plasmid#222958PurposeExpresses R36S K51S LoaP double mutantDepositorInsertantiterminator protein LoaP
UseTags10xHIS-SUMOExpressionBacterialMutationmutated Arginine 36 to Serine and Lysine 51 to Se…PromoterT7Available sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only