We narrowed to 30,447 results for: Ide

1. pET28a-Ec.coaBC (pCLK1210)

   Plasmid

   #50448

   Purpose

   Heterologous expression of E. coli CoA biosynthetic enzyme, PPCS_PPCDC - phosphopantothenoylcysteine synthetase_phosphopantothenoylcysteine decarboxylase

   Depositor

   Tadhg Begley, Erick Strauss

   Article

   Strauss et al J Biol Chem. 2001 Apr 27;276(17):13513-6

   Insert

   PPCS PPCDC
   

   Tags

   6XHIS tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEBTet_NAD-Snifit-mito

   Plasmid

   #113916

   Purpose

   Plasmid encoding the mitochondrial localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

   Insert

   NAD-Snifit
   

   Expression

   Mammalian

   Promoter

   CMV TetO2

   Available Since

   Sept. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-eNOS-GFP

   Plasmid

   #22444

   Depositor

   William Sessa

   Article

   Sowa et al J Biol Chem. 1999 Aug 6. 274(32):22524-3

   Insert

   WT eNOS-GFP
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Dec. 8, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMT447

   Plasmid

   #139387

   Purpose

   Bile Acid Inducible Nanoluc expression, pNBU1 backbone with BsaI sites, AmpR, ErmR

   Depositor

   Christopher Voigt

   Article

   Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

   Insert

   Bile Acid Inducible Nanoluc expression, pNBU1 backbone with BsaI sites
   

   Use

   Synthetic Biology

   Promoter

   Bile Acid inducible Promoter

   Available Since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET14-GFP15_Encap

   Plasmid

   #86407

   Purpose

   Expresses sfGFP tagged with a 15 amino acid CLP and encapsulin as an operon

   Depositor

   David Savage

   Article

   Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

   Inserts

   sfGFP with a 15aa CLP tag
   Encapsulin
   

   Tags

   6x Histidine

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEBTet_NAD-Snifit-cyto

   Plasmid

   #113914

   Purpose

   Plasmid encoding the cytosolic localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

   Insert

   NAD-Snifit
   

   Expression

   Mammalian

   Promoter

   CMV TetO2

   Available Since

   Sept. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. psiCHECK2-2A-3xFLAG-XBP1u_pause-2A

   Plasmid

   #159583

   Purpose

   Luciferase reporter for ribosome pausing

   Depositor

   Shintaro Iwasaki

   Article

   Han et al Cell Rep. 2020 May 5;31(5):107610. doi:

   Inserts

   XBP1u pause site
   EIF2S3 5UTR
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEBTet_NAD-Snifit-nucl

   Plasmid

   #113915

   Purpose

   Plasmid encoding the nuclear localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

   Insert

   NAD-Snifit
   

   Expression

   Mammalian

   Promoter

   CMV TetO2

   Available Since

   Sept. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMT451

   Plasmid

   #139391

   Purpose

   Bile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOT Gate 4), pNBU1 backbone, AmpR, ErmR

   Depositor

   Christopher Voigt

   Article

   Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

   Insert

   Bile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOT Gate 4)
   

   Use

   Synthetic Biology

   Promoter

   BA inducible promoters

   Available Since

   April 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEBTet_NADP-Snifit-nucl

    Plasmid

    #113918

    Purpose

    Plasmid encoding the nuclear localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

    Insert

    NADP-Snifit
    

    Expression

    Mammalian

    Promoter

    CMV TetO2

    Available Since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. LTR5HS S. aureus scaffold CARGO array

    Plasmid

    #191317

    Purpose

    Contains an array of guide RNAs targeting LTR5Hs elements

    Depositor

    Joanna Wysocka

    Article

    Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

    Insert

    Array of guide RNAs
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. psiCHECK2-2A-3xFLAG-SBP-2A

    Plasmid

    #159584

    Purpose

    Luciferase reporter for ribosome pausing control

    Depositor

    Shintaro Iwasaki

    Article

    Han et al Cell Rep. 2020 May 5;31(5):107610. doi:

    Inserts

    2A-3xFLAG-SBP-2A
    EIF2S3 5UTR
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMYCO1_SpdCas9_pmcDA1

    Plasmid

    #204615

    Purpose

    CRISPR cytosine deaminase replicative plasmid for Mycoplasma mycoides subsp. mycoides

    Depositor

    Pascal Sirand-Pugnet

    Article

    Ipoutcha et al Appl Environ Microbiol. 2022 Sep 13;88(1

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. RP hk339-GFP

    Plasmid

    #24431

    Depositor

    Ron Vale

    Article

    Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

    Insert

    Kinesin WT (KIF5B Human)
    

    Tags

    6XHis and GFP

    Expression

    Bacterial

    Mutation

    monomeric kinesin

    Available Since

    June 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL3-Gateway-DSCP

    Plasmid

    #71506

    Purpose

    flySTARR-seq validation vector with Gateway cassette upstream of the firefly luciferase gene and the DSCP core promoter

    Depositor

    Alexander Stark

    Article

    Arnold et al Science. 2013 Mar 1;339(6123):1074-7. do

    Type

    Empty backbone

    Use

    Flystarr-seq validation vector

    Tags

    luciferase

    Expression

    Insect

    Available Since

    Dec. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTUP1

    Plasmid

    #104341

    Purpose

    Express TUP1

    Depositor

    Alexander Varshavsky

    Article

    Xia et al J Biol Chem. 2008 Oct 24;283(43):28958-6

    Insert

    TUP1 (TUP1 Budding Yeast)
    

    Expression

    Yeast

    Available Since

    Dec. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. SLC30A10-GFP

    Plasmid

    #104381

    Purpose

    Expresses SLC30A10-GFP wild-type in mammalian cells

    Depositor

    Somshuvra Mukhopadhyay

    Article

    Selyunin et al J Cell Biol. 2017 Oct 2;216(10):3249-326

    Insert

    SLC30A10 (SLC30A10 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBGSA HA-FPR1

    Plasmid

    #62603

    Purpose

    Constitutive expression of HA-FPR1 in mammalian cells

    Depositor

    Heini Miettinen

    Article

    Gripentrog et al J Immunol. 2003 Sep 15;171(6):3187-93.

    Insert

    FPR1 (FPR1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    SRalpha

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pSSN6

    Plasmid

    #104340

    Purpose

    Express SSN6

    Depositor

    Alexander Varshavsky

    Article

    Xia et al J Biol Chem. 2008 Oct 24;283(43):28958-6

    Insert

    SSN6 (CYC8 Budding Yeast)
    

    Expression

    Yeast

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pXPR_212

    Plasmid

    #133457

    Purpose

    U6 promoter expresses customizable Spyo-guide; EFS promoter expresses SaurCas9 and 2A site provides puromycin resistance

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

    Insert

    control guide
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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