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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 23,988 results for: nes

Showing: 2521 - 2540 of 23988 results

  2. PGL4.11-COL1A1double mutants

    Plasmid
    #230918
    Purpose
    Double mutation of TEAD and SMAD binding elements in COL1A1 promoter; check the promoter activity.
    Depositor
    Avi Rosenberg
    Article
    Yang et al Lab Invest. 2024 May;104(5):100336. doi:
    Insert
    Human CLO1A1 promoter including SMAD and TEAD mutant elements (COL1A1 Human)
    Use
    Luciferase
    Tags
    Luciferase-luc2p
    Expression
    Bacterial and Mammalian
    Mutation
    COL1A1 promoter with mutant SMAD and TEAD binding…
    Promoter
    COL1A1 with mutant SMAD and TEAD elements
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230918-plasmid-map-sequence-id-460669

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  3. pαH-S-GSAS/EG.5.1

    Plasmid
    #218668
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein of OMICRON.EG.5.1 variant
    Depositor
    Priyamvada Acharya
    Article
    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.
    Insert
    pαH-S-GSAS/EG.5.1 (S )
    Use
    Tags
    HRV 3C cleavage site (before tags) C terminal on …
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    CMV
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218668-plasmid-map-sequence-id-433275

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  4. pαH-S-GSAS/XBB.1.5

    Plasmid
    #212991
    Purpose
    "Mammalian cell expression of SARS-CoV-2 Spike protein of Omicron.XBB.1.5 variant
    Depositor
    Priyamvada Acharya
    Article
    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.
    Insert
    pαH-S-GSAS/XBB.1.5 (S )
    Use
    Tags
    HRV 3C cleavage site (before tags) C terminal on …
    Expression
    Mammalian
    Mutation
    (T19I,Del24/26,A27S,V83A,G142D,Del144,H146Q,Q183E…
    Promoter
    CMV
    Available since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    212991-plasmid-map-sequence-id-422311

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  6. pαH-S-GSAS/EG.5

    Plasmid
    #212990
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein of OMICRON.EG5 variant
    Depositor
    Priyamvada Acharya
    Article
    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.
    Insert
    pαH-S-GSAS/EG.5 (S )
    Use
    Tags
    HRV 3C cleavage site (before tags) C terminal on …
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    CMV
    Available since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    212990-plasmid-map-sequence-id-423192

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  19. pαH-S-RRAR/XBB.1.5

    Plasmid
    #212993
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein of Omicron.XBB.1.5 with with furin side intact
    Depositor
    Priyamvada Acharya
    Article
    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.
    Insert
    Spike S-RRAR/XBB.1.5 (S )
    Use
    Tags
    HRV 3C cleavage site (before tags) C terminal on …
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); furin site intake; (T19I…
    Promoter
    CMV
    Available since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    212993-plasmid-map-sequence-id-423196

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  20. pC0050-CMV-dPspCas13b-longlinker-ADAR2DD(wt)

    Plasmid
    #103866
    Purpose
    dPspCas13b-ADAR2DD(wt) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Longer linker than pC0039.
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Inserts
    PspCas13b
    huADAR2DD (ADARB1 Human)
    Use
    CRISPR
    Tags
    GSGGGGS and HIV NES
    Expression
    Mammalian
    Mutation
    Changed histidne 133 to alanine and histidine 105…
    Promoter
    Available since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    103866-plasmid-map-sequence-id-199147

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Showing: 2521 - 2540 of 23988 results