We narrowed to 16,181 results for: nes
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Plasmid#60503PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of RFP, rtTA and neomycin resistance geneDepositorTypeEmpty backboneUseTransposonExpressionMammalianPromoterTCE & RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pYES-mtGFP
Plasmid#45053DepositorPublicationInsertmtGFP
ExpressionYeastPromoterGALAvailable sinceMay 24, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBID-UASC-VG
Plasmid#35206DepositorTypeEmpty backboneUseDrosophila transgene expressionTagsmVENUSPromoterDrosophila Synthetic Core Promoter (DSCP)Available sinceJuly 26, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TV-hPITX3-Reverse
Plasmid#22208DepositorAvailable sinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLA230
Plasmid#11724DepositorInsertb-lactamase
ExpressionBacterialMutationGAA for Glu26 changed to TAA (premature stop codo…Available sinceAug. 8, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFD116
Plasmid#124769PurposepFD116 carries dcas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vectorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsPtet
dcas9
PpflB sgRNA BsaI
UseCRISPRAvailable sinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
wtBxb1
Plasmid#222337PurposePlasmid for expressing wild type Bxb1 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCodon-optimized wild type Bxb1
ExpressionMammalianPromoterCMVAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti- V6.3 lox-dsRED-stop-lox-eGFP-blas
Plasmid#106171Purposecre mediated red to green shiftDepositorInsertsdsRED-express2
eGFP
UseCre/Lox and LentiviralExpressionMammalianPromoterCMVAvailable sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PKC gamma DN
Plasmid#21239DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPKC gamma (Prkcg Mouse)
TagsHAExpressionMammalianMutationK380RAvailable sinceAug. 13, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSEM238
Plasmid#161515PurposeNeuronal expression of histamine-sensitive chloride channel for negative selectionDepositorInsertpEXP[Psnt-1 | histamine | rpl-3 UTR]
ExpressionWormPromoterPsnt-1Available sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-PEmax
Plasmid#187893PurposeAll-in-one prime editor piggyBac transposon with PEmaxDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSpCas9_H840A_KK-MMLV-RT_co-P2A-PAC_dTK
UseCRISPR and Synthetic Biology; Piggybac transposonTagsBPSV40 NLS and c-Myc NLSExpressionMammalianPromoterCAGAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC95-pmax-dCas9VP160-2A-neo
Plasmid#48227PurposedCas9VP160-2A-neo (neo/G418-selectable) on pmax expression vector. Note: This is being tested.DepositorInsertdCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-neo
UseCRISPRTags2A-neo, HA Tag, and VP160ExpressionMammalianMutationD10A;H840A (catalytically inactive)PromoterCAGGSAvailable sinceSept. 27, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PKM2
Plasmid#25538PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable sinceAug. 24, 2010AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pSuperRetropuro-IL-6 shRNA 1
Plasmid#19959DepositorInsertpSuperRetropuro-IL-6 shRNA 1 (IL6 Human)
UseRNAi and RetroviralAvailable sinceJan. 12, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGL-FLKif3B
Plasmid#13743DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmouse Kif3B (Kif3b Mouse)
UseCre/LoxTagsGFPExpressionMammalianAvailable sinceJan. 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
pL-CRISPR.SFFV.PAC
Plasmid#57829PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, SFFV Promoter drivenDepositorInsertsSpCas9
Sp sgRNA scaffold
SFFV
P2A-PAC
UseCRISPR and LentiviralTagsFLAGAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONRp5-p2 SspB-mCherry-CShroom3
Plasmid#170977PurposeC-terminal component of OptoShroom3 optogenetic tool. Translocates to apical junctions to induce constriction upon blue light illumination if coexpressed with GFP-NShroom3-iLID. pDONRp5-p2 plasmid.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCShroom3 (Shroom3 Mouse)
UseGateway CloningTagsSspB and mCherryMutationIsoform X2, deleted aminoacids 1 - 1388Available sinceJuly 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
CD8a-EGFP
Plasmid#86051PurposeA mammalian expression plasmid encoding CD8a with a C-terminus EGFP.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCD8a (CD8A Human)
TagsGFPExpressionMammalianPromoterCMVAvailable sinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
OPN(-1206)-luc
Plasmid#31106DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertosteopontin promoter (SPP1 Human)
UseLuciferaseExpressionMammalianMutationpromoter region -1206 to +87Available sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCSW21
Plasmid#86836Purposebacterial expression of anti-CRISPR protein AcrIIA4DepositorInsertacrIIA4
ExpressionBacterialPromoteraraBADAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by