Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

177,692 results

Showing: 1271 - 1280 of 177692 results

  3. pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro

    Plasmid
    #141385
    Purpose
    Lentiviral expression of SARS-CoV-2 envelope (E) protein; transient expression and generate lentivirus
    Depositor
    Nevan Krogan
    Publication
    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:
    Insert
    SARS-CoV-2-E (E SARS-CoV-2)
    Use
    Lentiviral
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    EF1alpha
    Available since
    April 8, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    21 Articles
    141385-plasmid-map-sequence-id-288068

    Enlarge

  6. AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL)

    Plasmid
    #60224
    Purpose
    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and three U6-driven sgRNAs targeting Kras, p53, and Lkb1. Contains KrasG12D HDR donor. AAV backbone.
    Depositor
    Feng Zhang
    Publication
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Renilla luciferase
    Cre recombinase
    KrasG12D HDR donor (Kras Mouse)
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA and Rluc-P2A
    Expression
    Mammalian
    Promoter
    EFS, No promoter, and U6
    Available since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    9 Articles
    60224-plasmid-map-sequence-id-418949

    Enlarge

  8. CMV-B-GECO1

    Plasmid
    #32448
    Purpose
    Mammalian expression of blue fluorescent genetically encoded Ca2+ indicator for optical imaging
    Depositor
    Robert Campbell
    Publication
    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    B-GECO1.0
    Expression
    Mammalian
    Mutation
    GCaMP3 S40P/L60P/D86G/K119I/L173Q/T223S/Y224H/D30…
    Promoter
    CMV
    Available since
    Sept. 30, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    32448-plasmid-map-sequence-id-122739

    Enlarge

  9. pLenti-EF1a-hChR2(H134R)-EYFP-WPRE

    Plasmid
    #20942
    Purpose
    3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by EF1a promoter for optogenetic activation
    Depositor
    Karl Deisseroth
    Publication
    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.
    Insert
    channelrhodopsin-2
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    H134R mutation in ChR2
    Promoter
    EF1alpha
    Available since
    May 13, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    11 Articles
    20942-plasmid-map-sequence-id-476966

    Enlarge

Showing: 1271 - 1280 of 177692 results