164,577 results

1. pCAG-Synapto-pHluorin

   Plasmid

   #168510

   Purpose

   Expression of Synapto-pHluorin for optical monitoring of presynaptic release in neurons.

   Depositor

   Franck Polleux

   Article

   Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

   Insert

   Synaptophysin-pHluorin (Syp Mouse)
   

   Use

   Tags

   pHluorin

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   April 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMax_mOrange

   Plasmid

   #177827

   Purpose

   Host Cell Reactivation (HCR) control mOrange plasmid (pMax backbone)

   Depositor

   Zachary Nagel, Leona Samson

   Article

   Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

   Insert

   mOrange
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJV53

   Plasmid

   #26904

   Depositor

   Graham Hatfull

   Article

   van Kessel et al Nat Methods. 2007 Feb . 4(2):147-52.

   Insert

   Che9c genes 60-61
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-pCbh-SpRY[C-term]-gRNAentry (CA20)

   Plasmid

   #197511

   Purpose

   Cbh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpRY and BsmBI entry cassette to clone SpCas9 gRNA spacer

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   AAV-[ITR]-pCbh-BPNLS-NpuC-SpRY[C-term]-BPNLS-gRNA[BsmBI]-pU6-[ITR]
   

   Use

   AAV and CRISPR

   Tags

   BPNLS and BPNLS-NpuC(intein)

   Expression

   Mutation

   C-terminal mutations of SpRY(L1111R/D1135L/S1136W…

   Promoter

   Cbh

   Available Since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pInducer20-EGFP-TEADiv2

   Plasmid

   #228876

   Purpose

   Tet-inducible lentiviral vector for expression of optimized GFP-TEAD inhibitor (TEADiv2)

   Depositor

   Ramiro Iglesias-Bartolome

   Article

   Branch et al bioRxiv [Preprint]. 2024 Oct 3:2024.10.0

   Insert

   TEADiv2
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. HypoxCR

   Plasmid

   #59946

   Purpose

   A lentiviral dual fluorescent protein reporter, HypoxCR, detects hypoxic [hypoxia-inducible factor (HIF) active] and/or cycling cells.

   Depositor

   Anne Le

   Article

   Le et al Proc Natl Acad Sci U S A. 2014 Aug 11. p

   Inserts

   GFP-Pest
   mCherry-Geminin
   

   Use

   Lentiviral

   Tags

   FLAG and PEST

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and min CMV

   Available Since

   April 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. EGFP-LC3

   Plasmid

   #11546

   Depositor

   Karla Kirkegaard

   Article

   Jackson et al PLoS Biol. 2005 May . 3(5):e156.

   Insert

   LC3B (MAP1LC3B Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. qTAG-C-3xFLAG-Puro

   Plasmid

   #207728

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xFLAG-2A-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pCMV4-ApoE3

   Plasmid

   #87086

   Purpose

   Expresses ApoE3 in Mammalian cells

   Depositor

   Bradley Hyman

   Article

   Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

   Insert

   Apolipoprotein E3 (APOE Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFUW-tetO-Pu.1

    Plasmid

    #139839

    Purpose

    doxycycline-inducible expression of mouse Pu.1 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    Pu.1 (Spi1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-mCMV

    Available Since

    April 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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