We narrowed to 6,963 results for: RAP

1. piggyBac-rtTA (4th_Gen)-NFIB-SOX9-IRES-mKate2 (VK_1244)

   Plasmid

   #209082

   Purpose

   NFIB and SOX9 mediated astrocye induction

   Depositor

   Vikram Khurana

   Article

   Lam et al bioRxiv 2022.11.08.515615

   Inserts

   NFIB-P2A-SOX9-V5 (NFIB, SOX9 Human)
   mKate2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE4G

   Available since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLenti-EF1a-HIF2a dPA (P405A, P531A)

   Plasmid

   #232954

   Purpose

   Stably express a constitutively stable version of HIF2a (HIF2a dPA)

   Depositor

   William Kaelin

   Article

   Jiang et al Cell. 2025 Feb 25:S0092-8674(25)00156-4.

   Insert

   HIF2a (EPAS1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   P405A, P531A

   Promoter

   EF1a

   Available since

   March 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. piggyBac-rtTA (4th_Gen)-sfGFP-IRES-NGN2-puro (VK_1122)

   Plasmid

   #209079

   Purpose

   Ngn2 mediated cortical neuron induction, along with sfGFP over-expression

   Depositor

   Vikram Khurana

   Article

   Lam et al bioRxiv 2022.11.08.515615

   Inserts

   sfGFP
   NGN2 (Neurog2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE4G

   Available since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. MADR pDonor-H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

   Plasmid

   #131461

   Purpose

   MADR donor plasmid for FlpO+Cre-mediated insertion of WT H3F3A and oncogenic PDGFRA D842V and TRP53

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
   

   Use

   Cre/Lox and Synthetic Biology; Mosaic analysis fo…

   Tags

   Trp53-V5, H3F3A-EGFP-AU1

   Expression

   Mammalian

   Mutation

   Pdgfra D842V

   Promoter

   none (4x polyA to mitigate episomal expression)

   Available since

   July 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. MADR pDonor-H3F3A-K27M-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

   Plasmid

   #131462

   Purpose

   MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic H3F3A K27M, PDGFRA D842V, and TRP53

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   H3F3A-K27M-EGFP AU1 pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
   

   Use

   Cre/Lox and Synthetic Biology; Mosaic analysis fo…

   Tags

   Trp53-V5, H3F3A-EGFP-AU1

   Expression

   Mammalian

   Mutation

   H3F3A K27M, Pdgfra D842V

   Promoter

   none (4x polyA to mitigate episomal expression)

   Available since

   July 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MADR pDonor-H3F3A-G34R-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

   Plasmid

   #131463

   Purpose

   MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic H3F3A G34R, PDGFRA D842V, and TRP53

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   H3F3A-G34R-EGFP AU1 pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
   

   Use

   Cre/Lox and Synthetic Biology; Mosaic analysis fo…

   Tags

   Trp53-V5, H3F3A-EGFP-AU1

   Expression

   Mammalian

   Mutation

   H3F3A G34R, Pdgfra D842V

   Promoter

   none (4x polyA to mitigate episomal expression)

   Available since

   July 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Nsp3 Mac1 - C2 (UCSF)

   Plasmid

   #169209

   Purpose

   Expression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removal

   Depositor

   James S Fraser

   Article

   Schuller et al Sci Adv. 2021 Apr 14;7(16). pii: 7/16/ea

   Insert

   SARS-CoV-2 Nsp3 Mac1
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 28, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. MADR pDonor TagBFP2-V5-HRAS G12VWPRE

   Plasmid

   #129420

   Purpose

   MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic Hras G12V with a TagBFP2 fusion

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   TagBFP2-V5-HRAS G12V (HRAS Entacmaea quadricolor (TagBFP), Human)
   

   Use

   Mosaic analysis for dual recombinase-mediated cas…

   Tags

   TagBFP2-V5 tag

   Expression

   Mutation

   G12V

   Promoter

   none (3x polyA to mitigate episomal expression)

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. FUW-tetO-hOKMS

   Plasmid

   #51543

   Purpose

   Inducible expression of polycistronic 2A spaced OCT4-KLF4-MYC-SOX2

   Depositor

   Tarjei Mikkelsen

   Article

   Cacchiarelli et al Cell. 2015 Jul 16;162(2):412-24. doi: 10

   Insert

   hOKMS (MYC, KLF4, SOX2, POU5F1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetO

   Available since

   May 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Nsp3 Mac1 - P43 (Oxford)

    Plasmid

    #169210

    Purpose

    Expression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removal

    Depositor

    James S Fraser

    Article

    Schuller et al Sci Adv. 2021 Apr 14;7(16). pii: 7/16/ea

    Insert

    SARS-CoV-2 Nsp3 Mac1
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 28, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. MADR pDonor TagBFP2-V5-HRAS G12V WPRE RCE

    Plasmid

    #131730

    Purpose

    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic Hras G12V with a TagBFP2 fusion compatible with RCE:loxP mouse strain

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    TagBFP2-V5-HRAS G12V (HRAS Entacmaea quadricolor (TagBFP), Human)
    

    Use

    Cre/Lox and Synthetic Biology; Mosaic analysis fo…

    Tags

    TagBFP2-V5 tag

    Expression

    Mammalian

    Mutation

    G12V

    Promoter

    none (4x polyA to mitigate episomal expression)

    Available since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. piggyBac-rtTA (4th_Gen)-SNCA(A53T)-sfGFP-IRES-NGN2-puro (VK_1123)

    Plasmid

    #209080

    Purpose

    Ngn2 mediated cortical neuron induction, along with SNCA(A53T)-sfGFP over-expression

    Depositor

    Vikram Khurana

    Article

    Lam et al bioRxiv 2022.11.08.515615

    Inserts

    SNCA (SNCA Human)
    NGN2 (Neurog2 Mouse)
    

    Use

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    A53T

    Promoter

    TRE4G

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. piggyBac-rtTA (4th_Gen)-SNCA(3K)-sfGFP-IRES-NGN2-puro (VK_1120)

    Plasmid

    #209078

    Purpose

    Ngn2 mediated cortical neuron induction, along with SNCA(3K) over-expression

    Depositor

    Vikram Khurana

    Article

    Lam et al bioRxiv 2022.11.08.515615

    Inserts

    SNCA (SNCA Human)
    NGN2 (Neurog2 Mouse)
    

    Use

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    3K mutant: E35K, E46K, E61K

    Promoter

    TRE4G

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBiT3.1-N_MED16:1-860

    Plasmid

    #211215

    Purpose

    Mammalian expression of full-length human MED16 isoform 3. N-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    MED16:M1-P860 (MED16 Human)
    

    Use

    Tags

    MVSGWRLFKKISGSSGGSSG

    Expression

    Mammalian

    Mutation

    isoform 3

    Promoter

    CMV

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBiT3.1-C_MED16:1-860

    Plasmid

    #211289

    Purpose

    Mammalian expression of full-length human MED16 isoform 3. C-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    MED16:M1-P860 (MED16 Human)
    

    Use

    Tags

    GSSGGSSGVSGWRLFKKIS

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. piggyBac-rtTA (4th_Gen)-SNCA(A53T-DNAC)-sfGFP-IRES-NGN2-puro (VK_1124)

    Plasmid

    #209081

    Purpose

    Ngn2 mediated cortical neuron induction, along with SNCA(A53T)-DeltaNAC-sfGFP over-expression

    Depositor

    Vikram Khurana

    Article

    Lam et al bioRxiv 2022.11.08.515615

    Inserts

    SNCA (SNCA Human)
    NGN2 (Neurog2 Mouse)
    

    Use

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    A53T, NAC deletion (aa60-95)

    Promoter

    TRE4G

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPB-C6F/TdTomato

    Plasmid

    #140826

    Purpose

    OCT4, SOX2, KLF4, C-MYC, KLF2, NANOG (C6F) and tdTomato under the control of TRE (Tet-ON)

    Depositor

    Hideyuki Okano

    Article

    Kisa et al Stem Cell Reports. 2017 Dec 12;9(6):1825

    Inserts

    OCT4 (POU5F1 Human)
    SOX2 (SOX2 Human)
    KLF4 (KLF4 Human)
    C-MYC (MYC Human)
    KLF2 (KLF2 Human)
    NANOG (NANOG Human)
    tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L104P- please see depositor comments

    Promoter

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGL4.84(hRlucCP/Puro)

    Plasmid

    #212935

    Purpose

    RapidResponse(TM) Renilla luciferase vector for investigating regions controlling transcription

    Depositor

    Pete Stecha

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Jan. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pAAV_CKI(0.4)_HA_AzPPO_AgeI_nrxn1a_WT_IRES_mScarlet

    Plasmid

    #205289

    Purpose

    Bistable opsin for neuronal inhibition

    Depositor

    Matthias Prigge

    Insert

    Parapinopsin
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV_CKII(0.4)_AzPPO_D14_ts_mScarlet_Kv2.1

    Plasmid

    #205288

    Purpose

    Bistable opsin for neuronal inhibition

    Depositor

    Matthias Prigge

    Insert

    Parapinopsin
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTIN-ura4

    Plasmid

    #161808

    Purpose

    A ura-selectable lncRNA-regulated gene expression system with rapid induction kinetics in the fission yeast Schizosaccharomyces pombe

    Depositor

    Stewart Shuman

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Pho1

    Available since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. MZB223

    Plasmid

    #228491

    Purpose

    Empty C-terminal 8xHis tag vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression

    Depositor

    Richard Baker

    Type

    Empty backbone

    Use

    Tags

    8xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. MZB184

    Plasmid

    #228488

    Purpose

    Empty 10xHis-SUMO (Smt3) vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression

    Depositor

    Richard Baker

    Type

    Empty backbone

    Use

    Tags

    10xHis-SUMO(Smt3)

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. MZB116

    Plasmid

    #228480

    Purpose

    "Empty" C-terminal self-cleaving CPD-tag vector for rapid cloning/DNA Assembly via SwaI restriction enzyme site (bacterial expression)

    Depositor

    Richard Baker

    Type

    Empty backbone

    Use

    Tags

    CPD-10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. MZB133

    Plasmid

    #228484

    Purpose

    "Empty" N-terminal TEV cleavable 6x His tag vector for rapid cloning/DNA Assembly via SmaI restriction site (Bacterial Expression)

    Depositor

    Richard Baker

    Type

    Empty backbone

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. MZB117

    Plasmid

    #228481

    Purpose

    "Empty" C-terminal HRV-3C cleavable GST tag vector for rapid cloning/DNA Assembly via NheI restriction enzyme site (Bacterial Expression)

    Depositor

    Richard Baker

    Type

    Empty backbone

    Use

    Tags

    GST (glutathione S-transferase)

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. myc mTOR kinase dead

    Plasmid

    #8482

    Depositor

    David Sabatini

    Insert

    mTOR (Mtor Rat)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    D2357E V2364I

    Promoter

    Available since

    June 20, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. MZB186

    Plasmid

    #228489

    Purpose

    Empty 10xHis-SUMO (Smt3) fused Avi-tag vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression

    Depositor

    Richard Baker

    Type

    Empty backbone

    Use

    Tags

    10xHis-SUMO(Smt3)-Avitag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEGFP-TSAd-wt

    Plasmid

    #84562

    Purpose

    mammalian expression of TSAd-EGFP fusion

    Depositor

    Anne Spurkland

    Insert

    TSAd (SH2D2A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MZB174

    Plasmid

    #228487

    Purpose

    10xHis-SUMO (Smt3) fused, HRV-3C cleavable, GST-tag for rapid cloning/DNA assembly of pulldown targets via NotI restriction site for E. coli based protein expression

    Depositor

    Richard Baker

    Type

    Empty backbone

    Use

    Tags

    10xHis-SUMO(Smt3)-GST-HRV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pOpen-T7gene5

    Plasmid

    #165534

    Purpose

    DNA Pol with high fidelity and rapid extension rate (useful in copying long stretches of DNA); Has strong 3'-5' exonuclease.

    Depositor

    Drew Endy, Jennifer Molloy, FreeGenes Project

    Insert

    T7 DNA Polymerase (unmodified)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pSLfa[Hsp_p-GAL4Delta-SV40;attp]fa

    Plasmid

    #86451

    Purpose

    Gal4 driver construct for misexpression in Tribolium with Tribolium basal promoter; has been used for enhancer trapping; (Shuttle plasmid)

    Depositor

    Gregor Bucher

    Insert

    Hsp_p-GAL4Delta-SV40;attp
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. MZB205

    Plasmid

    #228490

    Purpose

    Empty 10xHis-SUMO (Smt3) fused Avi-tag, HRV-3C cleavable vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression

    Depositor

    Richard Baker

    Type

    Empty backbone

    Use

    Tags

    10xHis-SUMO(Smt3)-Avitag-HRV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3-TRAM-CFP

    Plasmid

    #13027

    Depositor

    Doug Golenbock

    Insert

    Toll-like Receptor Adaptor Molecule (TICAM2 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. R777-E261 Hs.RASSF5

    Plasmid

    #70545

    Purpose

    Gateway ORF clone of human RASSF5 [NM_182665.3] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    RASSF5 (RASSF5 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. GuideVector_BbsI

    Plasmid

    #110692

    Purpose

    For rapid cloning and expression of sgRNAs in mammalian cells. Contains copGFP for easy FACS sorting.

    Depositor

    Douglas Mahoney

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. R777-E262 Hs.RASSF5-nostop

    Plasmid

    #70546

    Purpose

    Gateway ORF clone of human RASSF5 [NM_182665.3] without stop codon (for C-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    RASSF5 (RASSF5 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. A5TSR

    Plasmid

    #50358

    Purpose

    expresses T.gondii MIC2(67-593) domains in mammalian cells

    Depositor

    Timothy Springer

    Insert

    MIC2-A5TSR
    

    Use

    Tags

    HIS

    Expression

    Mammalian

    Mutation

    S158A, N357S, N463S, N470D

    Promoter

    CHICK beta-actin promoter

    Available since

    March 14, 2014

    Availability

    Academic Institutions and Nonprofits only

39. pPMS-1791

    Plasmid

    #232286

    Purpose

    Protein Expression

    Depositor

    Jeremy Nathans

    Insert

    Apcdd1 (Apcdd1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pTRERF1-donor

    Plasmid

    #176366

    Purpose

    CRISPR donor plasmid to create GFP fusion proteins

    Depositor

    Kevin White, Barbara Stranger

    Insert

    TRERF1 (TRERF1 Human)
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. TBXTA-c005

    Plasmid

    #139754

    Purpose

    For protein expression/crystallography (PDB:6F58)

    Depositor

    Opher Gileadi

    Insert

    TBXT, DNA-binding domain, WT (TBXT Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Contains only amino acids E41-S224

    Promoter

    T7

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. A6TSR

    Plasmid

    #50359

    Purpose

    expresses T.gondii MIC2(67-660) domains in mammalian cells

    Depositor

    Timothy Springer

    Insert

    MIC2-A6TSR
    

    Use

    Tags

    HIS

    Expression

    Mammalian

    Mutation

    S158A, N357S, N463S, N470D

    Promoter

    CHICK beta-actin promoter

    Available since

    March 14, 2014

    Availability

    Academic Institutions and Nonprofits only

43. pBS L30 mRuby3-Rab27A

    Plasmid

    #166432

    Purpose

    Rab27A fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.

    Depositor

    Raphael Gaudin

    Insert

    Rab27A
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    L30 weak promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pBS L30 mRuby3-Rab18

    Plasmid

    #166431

    Purpose

    Rab18 fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.

    Depositor

    Raphael Gaudin

    Insert

    Rab18
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    L30 weak promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pBS L30 mRuby3-Rab6b

    Plasmid

    #166427

    Purpose

    Rab5C fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.

    Depositor

    Raphael Gaudin

    Insert

    Rab6B
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    L30 weak promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pBS L30 mRuby3-Rab10

    Plasmid

    #166429

    Purpose

    Rab10 fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.

    Depositor

    Raphael Gaudin

    Insert

    Rab10
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    L30 weak promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. RABGEF1A-c014

    Plasmid

    #175478

    Purpose

    Protein expression in insect cells. Entire CDS, M1-G491. Codon optimised.

    Depositor

    Opher Gileadi

    Insert

    RABGEF1 (RABGEF1 Human)
    

    Use

    Tags

    His6-Zbasic-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pBS L30 mRuby3-Rab8A

    Plasmid

    #166428

    Purpose

    Rab8A fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.

    Depositor

    Raphael Gaudin

    Insert

    Rab8A
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    L30 weak promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. TBXTA-c006

    Plasmid

    #139755

    Purpose

    For protein expression/crystallography (PDB:6F59)

    Depositor

    Opher Gileadi

    Insert

    TBXT, DNA-binding domain, G177D (TBXT Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    G177D; contains only amino acids E41-S224

    Promoter

    T7

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pBS L30 mRuby3-Rab11A

    Plasmid

    #166430

    Purpose

    Rab11A fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.

    Depositor

    Raphael Gaudin

    Insert

    Rab11A
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    L30 weak promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge