We narrowed to 64,328 results for: %s

Showing: 2541 - 2560 of 64328 results

Results per page:

Sort results by:

pDDGFP_LEU2D

Plasmid

#58352

Purpose

Used for expressing recombinant proteins as a C terminal GFP-His tagged constuct in S.cerevisiae

Depositor

Simon Newstead

Article

Parker et al Protein Sci. 2014 Jun 20. doi: 10.1002/p

Type

Empty backbone

Tags

GFP-His

Expression

Yeast

Promoter

GAL1

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSETa_ mEos4b

Plasmid

#51073

Purpose

Photoconverible Fluorescent Protein_ Fixative Resistant (monomeric)

Depositor

Loren Looger

Article

Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

mEos4b

Expression

Bacterial

Promoter

T7

Available Since

Feb. 19, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
M-ST1n-VP64

Plasmid

#48675

Purpose

Mammalian ST1-VP64 nuclease-null Cas9 activator expression, human optimized

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Cas9-VP64, nuclease-null

Use

CRISPR

Tags

NLS and VP64

Mutation

nuclease-null (D9A D598A H599A N622A)

Promoter

CMV

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pLv-HSA-uDys/eGFP

Plasmid

#26810

Depositor

Jeffrey Chamberlain

Article

Kimura et al Hum Mol Genet. 2008 Aug 15. 17(16):2507-

Insert

dystrophin (DMD Human)

Use

Lentiviral; Lentiviral plasmid backbone from l. n…

Tags

eGFP

Mutation

deleted R4-R23 deleted exons 71-78

Available Since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-Flag-hKlf4

Plasmid

#20074

Depositor

Juan Belmonte

Article

Aasen et al Nat Biotechnol. 2008 Nov . 26(11):1276-8

Insert

hKlf4 (KLF4 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
phREX1-Luc

Plasmid

#17222

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

Insert

Upstream region of human REX1 (ZFP42 Human)

Use

Luciferase

Expression

Mammalian

Mutation

None

Available Since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pLI50

Plasmid

#13573

Depositor

Chia Lee

Article

Lee et al Gene. 1991 Jul 15. 103(1):101-5.

Type

Empty backbone

Use

S. aureus-e. coli shuttle vector

Available Since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-2TK-14-3-3 eta GST

Plasmid

#13277

Depositor

Michael Yaffe

Article

Yaffe et al Cell. 1997 Dec 26. 91(7):961-71.

Insert

14-3-3 eta (YWHAH Human)

Tags

GST

Expression

Bacterial

Available Since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only
pAAV-EF1a-iCreV

Plasmid

#140135

Purpose

Can be used to generate AAV virus that will express light-inducible site-specific iCreV recombinase

Depositor

Hongkui Zeng

Article

Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

Has Service

AAV PHP.eB

Insert

iCreV

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1alpha

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-TO-hNIL

Plasmid

#172113

Purpose

Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Article

Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

Inserts

hNGN2 (NEUROG2 Human)
hISL1 (ISL1 Human)
hLHX3 (LHX3 Human)
puroR-T2A-mycNLS-mTagBFP2

Tags

T2A-mycNLS-mTagBFP2

Expression

Mammalian

Promoter

EF1a and TRE3G

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p4xmts-mScarlet3_N1

Plasmid

#189774

Purpose

Construct for labelling mitochondria in mammalian cells using mitochondrial targeted mScarlet3

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

4xmts-mScarlet3

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-HyPer7-MEM

Plasmid

#136465

Purpose

Mammalian expression of cytosolic side of plasma membrane targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Tags

Farnselation tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Artichoke

Plasmid

#73320

Purpose

Artichoke is a protein degradation reporter plasmid in a lentiviral backbone (SFFV.BsmBIClonesite.17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)

Depositor

Benjamin Ebert

Article

Slabicki et al Nature. 2020 Sep;585(7824):293-297. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

SFFV and EF1a

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA6 asyn WT

Plasmid

#107425

Purpose

Mammalian expression of human alpha synuclein

Depositor

Hilal Lashuel

Article

Mbefo et al J Biol Chem. 2015 Apr 10;290(15):9412-27

Insert

alpha-synuclein (SNCA Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-3xFLAG-TEV-UBE2O

Plasmid

#105718

Purpose

Expresses WT full length human N- terminally tagged UBE2O

Depositor

Ramanujan Hegde

Article

Yanagitani et al Science. 2017 Aug 4;357(6350):472-475. d

Insert

UBE2O (UBE2O Human)

Tags

3xFLAG-TEV

Expression

Mammalian

Promoter

CMV (from vector)

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK02

Plasmid

#105133

Purpose

Clostridium difficile CRISPR-cas9 mutagenesis plasmid traJ oriT

Depositor

Joseph Sorg

Article

McAllister et al Sci Rep. 2017 Nov 7;7(1):14672. doi: 10.

Inserts

Upstream & Downstream pyrE deletion region
tetR
Cas9
gRNA

Use

E. coli - c. difficile shuttle vector

Available Since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR-tagBFP

Plasmid

#75160

Purpose

Derived from LentiCRISPR v1 but expresses tagBFP instead of puromycin resistance

Depositor

Beat Bornhauser

Article

McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

tagBFP

Expression

Mammalian

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E1B

Plasmid

#64221

Purpose

Expression vector for sgRNA and for Expression of Cas9 linked via T2A to mCherry and to Ad4 E1B55K via P2A

Depositor

Ralf Kuehn

Article

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
sgRNA cassette

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-mCherry-P2A-E1B

Expression

Mammalian

Promoter

CBh and U6

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-CHRNA7

Plasmid

#62276

Purpose

mammalian expression of human Alpha7 (CHRNA7)

Depositor

Sherry Leonard, Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

CHRNA7 (CHRNA7 Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 21, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
Tet-inducible mCherry reporter

Plasmid

#64128

Purpose

Photoactivatable transcription system. mCherry reporter under the control of a tet-inducible promoter

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

mCherry

Expression

Mammalian

Promoter

TetOx13-CMV

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge