We narrowed to 4,418 results for: 424

1. MAC_C_CH60

   Plasmid

   #111662

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   CH60 (HSPD1 Human)
   

   Expression

   Mammalian

   Available Since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. p6600 MSCV-IP N-HAonly JUN

   Plasmid

   #34898

   Depositor

   Peter Howley

   Article

   White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

   Insert

   JUN (JUN Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   MSCV LTR

   Available Since

   Feb. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pAAV-hSynapsin1-FLEx-axon-GCaMP6s-P2A-mRuby3

   Plasmid

   #112008

   Purpose

   For cre-dependent enriched expression of GCaMP6s in axons with cytosolic expression of mRuby3

   Depositor

   Lin Tian

   Article

   Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

   Insert

   axon-GCaMP6s-P2A-mRuby3
   

   Use

   AAV and Cre/Lox

   Tags

   GAP43 palmitoylation domain

   Expression

   Mammalian

   Promoter

   hSynapsin1

   Available Since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. PD-1-miSFIT-4x

   Plasmid

   #124678

   Purpose

   Tuning expression of PD-1

   Depositor

   Tudor Fulga

   Article

   Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

   Insert

   PD-1 (PDCD1 Human)
   

   Expression

   Mammalian

   Promoter

   minimal CMV

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLJC6-GLS-3xFLAG

   Plasmid

   #163450

   Purpose

   lentiviral overexpression vector for human GLS

   Depositor

   Jason Cantor

   Article

   Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

   Insert

   GLS (GLS Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   See: comments

   Promoter

   Ubc

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. POLD1_pLX307

   Plasmid

   #98358

   Purpose

   Lentiviral expression of POLD1

   Depositor

   Sefi Rosenbluh, William Hahn

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   POLD1 (POLD1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   E1Fa

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. LiPOP2

   Plasmid

   #168266

   Purpose

   A single-component optogenetic tool for photoswitchable pyroptosis

   Depositor

   Yubin Zhou

   Article

   He et al Adv Sci (Weinh). 2021 May 6;8(13):210042

   Insert

   mGSDMD(1-276) (Gsdmd Mouse)
   

   Tags

   cpLOV2, mCherry

   Expression

   Mammalian

   Mutation

   R138A, K146A, R152A, R154A

   Promoter

   CMV

   Available Since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYES2/25Q

   Plasmid

   #1384

   Depositor

   Michael Sherman

   Article

   Meriin et al J Cell Biol 2002 Jun 10;157(6):997-1004.

   Insert

   huntingtin (25Q) (HTT Human)
   

   Tags

   EGFP and FLAG

   Expression

   Yeast

   Mutation

   Exon1 sequences containing the first 17 amino aci…

   Available Since

   Feb. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. CMV-YE1-BE3-FNLS-CMV-mCherry

   Plasmid

   #154005

   Purpose

   Express YE1-BE3-FNLS

   Depositor

   Hui Yang

   Article

   Zuo et al Nat Methods. 2020 May 18. pii: 10.1038/s

   Insert

   YE1-BE3-FNLS
   

   Use

   CRISPR

   Tags

   FLAG

   Promoter

   CMV

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP-CHC17KDP

    Plasmid

    #59799

    Purpose

    Expresses knockdown-proof human CHC17 tagged with GFP.

    Depositor

    Stephen Royle

    Article

    Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10

    Insert

    CHC17 (CLTC Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Silent mutations in amino acids 61-65 to confer s…

    Promoter

    CMV

    Available Since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. Flag‐hTim1

    Plasmid

    #49207

    Purpose

    expresses Flag-tagged human Tim1

    Depositor

    Lawrence Kane

    Article

    de Souza et al Proc Natl Acad Sci U S A. 2005 Nov 22;10

    Insert

    Tim1 (HAVCR1 Human)
    

    Tags

    FLAG and signal sequence

    Expression

    Mammalian

    Mutation

    Tim1 lacks its own signal sequence

    Promoter

    EF1a

    Available Since

    Dec. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pCR1163

    Plasmid

    #111099

    Purpose

    Expresses FANCD2_+_10068200.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    FANCD2 gRNA (FANCD2 Human)
    

    Use

    Lentiviral

    Available Since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMRX-IP/SECFP-hATG16A1

    Plasmid

    #58994

    Purpose

    Expresses SECFP-hATG16A1 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

    Insert

    Homo sapiens autophagy related 16-like 1 (S. cerevisiae) (ATG16L1 Human)
    

    Use

    Retroviral

    Tags

    SECFP

    Available Since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFUW-TetO-Junb

    Plasmid

    #178441

    Purpose

    doxycycline-inducible expression of Mouse Junb in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

    Insert

    Junb (Junb Mouse)
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP-ATF6-(S1P-)

    Plasmid

    #32956

    Purpose

    For visualization of ATF6 in mammalian cells with a mutation in ATF6 that blocks cleavage by protease S1P. Activated protein will remain in the Golgi.

    Depositor

    Ron Prywes

    Article

    Chen et al J Biol Chem. 2002 Apr 12. 277(15):13045-

    Insert

    ATF6 (ATF6 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S1Protease site mutation: R415A/R416A

    Promoter

    CMV

    Available Since

    Feb. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCGN-ATF6 (1-373)m1

    Plasmid

    #27174

    Purpose

    For mammalian expression of the cytoplasmic domain of human ATF6 with mutations eliminating DNA binding and with an HA tag.

    Depositor

    Ron Prywes

    Article

    Wang et al J Biol Chem. 2000 Sep 1. 275(35):27013-2

    Insert

    ATF6 (ATF6 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Contains only aa 1-373 Amino acids 315–317 are c…

    Available Since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

17. eGFP-KIF1A-Rigor

    Plasmid

    #172207

    Purpose

    Expresses KIF1A G251A rigor mutant tagged with eGFP in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Guardia et al Cell Rep. 2016 Nov 15;17(8):1950-1961. d

    Insert

    KIF1A (Kif1a Mouse)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Changed Glycine 251 to Alanine

    Promoter

    CMV

    Available Since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. EGFP-IpgD

    Plasmid

    #183666

    Purpose

    N-terminally tagged Shigella flexneri IpgD for mammalian expression

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    IpgD
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330-PITCh-CLTC

    Plasmid

    #227312

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of CLTC for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of CLTC (CLTC Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28C-mCherry-HNRNPA1LCD

    Plasmid

    #204405

    Purpose

    E. coli expression of HNRNPA1 low-complexity domain (186-320) with an N-terminal mCherry tag

    Depositor

    Samie Jaffrey

    Article

    Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

    Insert

    HNRNPA1 (186-320) (HNRNPA1 Human)
    

    Tags

    6x-His; mCherry

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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