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pAG_PluxB_ToeRep_N01_trigger

Plasmid

#132736

Purpose

Inducible expression of first-generation toehold repressor index 1 trigger RNA via the PluxB promoter

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

PluxB_ToeRep_N01_trigger

Expression

Bacterial

Available Since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pJK_ToeRepG2_N19_switch

Plasmid

#132744

Purpose

T7 RNAP-driven expression of second-generation toehold repressor index 19 switch RNA with a GFP-ASV reporter

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

ToeRepG2_N19_switch

Expression

Bacterial

Available Since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pJK_ToeRepG2_N64_switch

Plasmid

#132743

Purpose

T7 RNAP-driven expression of second-generation toehold repressor index 64 switch RNA with a GFP-ASV reporter

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

ToeRepG2_N64_switch

Expression

Bacterial

Available Since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pYZ_PluxB_3WJrep_N19_trigger

Plasmid

#132738

Purpose

Inducible expression of 3WJ repressor index 19 trigger RNA via the PluxB promoter

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

PluxB_3WJrep_N19_trigger

Expression

Bacterial

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pYZ_3WJrep_N13_trigger

Plasmid

#132735

Purpose

T7 RNAP-driven expression of 3WJ repressor index 13 trigger RNA

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

3WJ_N13_trigger

Expression

Bacterial

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pYZ_3WJrep_N12_trigger

Plasmid

#132734

Purpose

T7 RNAP-driven expression of 3WJ repressor index 12 trigger RNA

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

3WJ_N12_trigger

Expression

Bacterial

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pYZ_3WJrep_N24_trigger

Plasmid

#132729

Purpose

T7 RNAP-driven expression of 3WJ repressor index 24 trigger RNA

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

3WJ_N24_trigger

Expression

Bacterial

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pYZ_3WJrep_N19_trigger

Plasmid

#132727

Purpose

T7 RNAP-driven expression of 3WJ repressor index 19 trigger RNA

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

3WJ_N19_trigger

Expression

Bacterial

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pJW4.31

Plasmid

#131371

Purpose

expression of GST:MEG-1 in C. elegans

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

meg-1 (meg-1 Nematode)

Expression

Bacterial

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pJW4.13

Plasmid

#131375

Purpose

expression of MBP:PPTR-2 in C. elegans

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

pptr-2 (pptr-2 Nematode)

Expression

Bacterial

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pJW4.12

Plasmid

#131374

Purpose

expression of MBP:PPTR-1 in C. elegans

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

pptr-1 (pptr-1 Nematode)

Expression

Bacterial

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2+ rat MYO1D

Plasmid

#118419

Purpose

For mammalian expression of rat myosin 1D

Depositor

Michael Tsang

Article

Saydmohammed et al Nat Commun. 2018 Aug 23;9(1):3381. doi:

Insert

rat myosin 1D (Myo1d Rat)

Expression

Mammalian

Available Since

Sept. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pRS-ATG1(mut)(406)

Plasmid

#122069

Purpose

Expressing a mutant ATG1

Depositor

Daniel Klionsky

Article

Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

Insert

ATG1 (ATG1 Budding Yeast)

Expression

Yeast

Mutation

Codon changes without changing the amino acid seq…

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pRS-ATG7p-ATG1(406)

Plasmid

#122071

Purpose

Expressing ATG1

Depositor

Daniel Klionsky

Article

Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

Insert

ATG1 (ATG1 Budding Yeast)

Expression

Yeast

Mutation

The native promoter was replaced by ATG7 promoter…

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pRS-Dhh1-PA(D195A,E196A)(405)

Plasmid

#122065

Purpose

Expressing Dhh1-PA

Depositor

Daniel Klionsky

Article

Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

Insert

Dhh1-PA (DHH1 Budding Yeast)

Tags

PA

Expression

Yeast

Mutation

Aspartic acid 195 to alanine; Glutamic acid 196 t…

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pRS-Dhh1-PA(S226A,T228A)(405)

Plasmid

#122066

Purpose

Expressing Dhh1-PA

Depositor

Daniel Klionsky

Article

Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

Insert

Dhh1-PA (DHH1 Budding Yeast)

Tags

PA

Expression

Yeast

Mutation

Serine 226 to alanine; Threonine 228 to alanine

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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gnu-GFP 9A

Plasmid

#113006

Purpose

P element vector for transposon insertion of gnu-GFP 9A into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2017 May 30;6. doi: 10.7554/eLife

Insert

full length gnu (gnu Fly)

Use

P insertion vector

Tags

GFP

Mutation

9 CycB/CDK1 sites mutated to A

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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gnu-GFP WT

Plasmid

#113005

Purpose

P element vector for transposon insertion of gnu-GFP WT into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2017 May 30;6. doi: 10.7554/eLife

Insert

full length gnu (gnu Fly)

Use

P insertion vector

Tags

GFP

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX6P-1 gnu 9A

Plasmid

#113000

Purpose

For protein expression and purification of full-length Drosophila gnu 9A

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2017 May 30;6. doi: 10.7554/eLife

Insert

full length gnu (gnu Fly)

Expression

Bacterial

Mutation

9 CycB/CDK1 sites mutated to A

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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full length png in pGEX4t

Plasmid

#112974

Purpose

For protein expression and purification of full-length Drosophila png

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2017 May 30;6. doi: 10.7554/eLife

Insert

png (png Fly)

Tags

His

Expression

Bacterial

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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