We narrowed to 5,792 results for: actin
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Plasmid#217332PurposepiggyBac expression of multiAsCas12a-KRABDepositorInsertmultiAsCas12a
UseCRISPRTags6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2ExpressionMammalianMutationR1226A/E174R/S542R/K548RPromoterCAGAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-Sox2AV-T2A-Klf4-E2A-Myc
Plasmid#210017Purposeepisomal SKM (expressing mouse Sox2-A61V + Klf4 + Myc) for integration-free naïve reset.DepositorExpressionMammalianMutationSox2A61V is a point mutant of Sox2 that enhances …PromoterCAGAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.CAG.(cyto).cpSFGFP.HaloTag
Plasmid#209654PurposeNon-responsive controlDepositorInsertcpSFGFP.HaloTag
UseAAVPromoterCAGAvailable sinceNov. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-flex-GFP-4x6T
Plasmid#196418PurposeCre-dependent AAV expression of GFP preferentially in astrocytes; astrocyte selectivity generated with 4x6T miRNA targeting cassetteDepositorInsertGFP
UseAAV and Cre/Lox; Astrocyte-selectiveExpressionMammalianPromoterCAGAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CAG-DIO-pAceR-Kv2.1PR
Plasmid#195534PurposeCre-dependent red fluorescent, positive response-polarity voltage indicator; soma-targetedDepositorInsertpAceR-Kv2.1 proximal restriction sequence
UseAAV and Cre/LoxExpressionMammalianMutationVARNAM 78E, 81D, 92N; SI linkerPromoterCAGAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro CAG CLTX-NKG2D-2B4-CD3z CAR
Plasmid#157744Purposeknock CLTX-NKG2D-2B4-CD3z CAR into AAVS1 safe harbor locus for constitutive expressionDepositorInsertchlorotoxin containing chimeric antigen receptor targeting glioblastoma
UseCRISPR, Synthetic Biology, and TALENExpressionMammalianPromoterCAGAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Tol2-CAG::Nucbow
Plasmid#158992PurposeMulticolor nuclear-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing H2B-tethered mEYFP, mCherry or mCerulean upon Cre recombination)DepositorInsertNucbow
TagsAll FPs are fused to H2BExpressionMammalianPromoterCAGAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCX-SpiCee-NLS
Plasmid#140900Purposemammalian expression of SpiCee-NLS, a nucleus-restricted variant of the calcium buffer SpiCee that prevents downstream signalingDepositorInsertSpiCee-NLS
TagsmRFPExpressionMammalianPromoterCAGAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-Tet-on Advanced -BGH-PA
Plasmid#114379PurposeExpress reverse tetracycline inducible rtTA transcriptional activatorDepositorInsertrtTA and BGH-pA
ExpressionMammalianPromoterCAGAvailable sinceDec. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-FLInChR-mVenus
Plasmid#119298PurposeFLinChR is the fusion signal sequence and transmembrane (TM) domain of Neurexin 1B-delta to ChR2E123T/T159C for inversion ChR2 to an optogenetic inhibitorDepositorInsertNx1BTM-FCS-ChR2ET/TC-mVenus
UseAAVTagsmVenusPromoterCAGAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEM705 Gso (Ser)
Plasmid#109353PurposeGsX chimera with Gαo C-terminus (Pertussis toxin insensitive mutant)DepositorInsertGso chimera
TagsNoneExpressionMammalianMutationChanged Cysteine at position 391 to Serine (Pertu…PromoterCAGAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT2-CAG-fGFP
Plasmid#108714PurposeTransposon donor plasmid for long-term expression of membrane targetted GFPDepositorInsertEGFP
Tags6xHis and farnesylation signalExpressionMammalianPromoterCAGAvailable sinceMay 10, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.CAG-FLEX.SF-iGluSnFR.A184V.mRuby3
Plasmid#106205PurposeMedium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-iGluSnFR.A184V
UseAAVMutationGltI: A184V + mRuby3 fusionPromoterCAGAvailable sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CAG-Cas9
Plasmid#89995PurposeExpresses high levels of WT Cas9 for genome editingDepositorInserthCas9
Tags3xFlag, SV40 NLS, and T2A peptideExpressionMammalianPromoterCAGAvailable sinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CAG sHRPb-FKBP-post mGRASP
Plasmid#73146PurposeSmall fragment of split HRP and FKBP fused to the extracellular terminus of the "post mGRASP" scaffold published by Magee, in which the majority of the NLG1 extracellular domain is deleted.DepositorInsertsHRPb-FKBP-post mGRASP
TagsHA tagExpressionMammalianPromoterCAGAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCX-Klf4
Plasmid#66656PurposeFor expression of mouse Klf4.DepositorAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-HA-rat Nrxn1beta AS4(-)
Plasmid#58267PurposeExpression plasmid for HA-tagged rat Neurexin 1beta, lacking the insert at alternatively spliced segment 4 (AS4-)DepositorAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-GFPmG1
Plasmid#50444PurposeExpression of a EGFP variant in mammalian cellsDepositorInsertGFPmG1
ExpressionMammalianMutationE143K, N147QPromoterCAGAvailable sinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN-SBP-DICER1
Plasmid#50558Purposepurification of Dicer1 from mammalian cellsDepositorAvailable sinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLH_Scr15
Plasmid#100537PurposeCEN/ARS plasmid with expression cassettes for PhyBNT-CreN and PIF3-NLS-CreC fusion proteins for red/far-red light-regulated Cre recombinase activity in Saccharomyces cerevisiae.DepositorUseCre/Lox and Synthetic BiologyTagsCreC, CreN, and SV40 NLSExpressionYeastMutationN-terminal version of PhyB (AA 1-621)/ Mutation o…PromoterFBA1 and TDH3Available sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by