Addgene

We narrowed to 18,217 results for: Met;

Showing: 2561 - 2580 of 18217 results

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MLM2579

Plasmid

#43891

Purpose

Activation Domain: NF-kB; RVD of C-terminal 0.5 TALE repeat: NG (binds T)

Depositor

Keith Joung

Article

Maeder et al Nat Methods. 2013 Feb 10. doi: 10.1038/n

Insert

TALE-activator p65 backbone

Use

Tale transcriptional activator expression vector

Tags

Expression

Mutation

Promoter

EF1alpha

Available since

March 8, 2013

Availability

Academic Institutions and Nonprofits only

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MLM2583

Plasmid

#43889

Purpose

Activation Domain: NF-kB; RVD of C-terminal 0.5 TALE repeat: HD (binds C)

Depositor

Keith Joung

Article

Maeder et al Nat Methods. 2013 Feb 10. doi: 10.1038/n

Insert

TALE-activator p65 backbone

Use

Tale transcriptional activator expression vector

Tags

Expression

Mutation

Promoter

EF1alpha

Available since

March 8, 2013

Availability

Academic Institutions and Nonprofits only

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pAAVf-EnhCB-lacZnls

Plasmid

#35642

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Type

Empty backbone

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

April 25, 2012

Availability

Academic Institutions and Nonprofits only

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MLM2581

Plasmid

#43888

Purpose

Activation Domain: NF-kB; RVD of C-terminal 0.5 TALE repeat: NI (binds A)

Depositor

Keith Joung

Article

Maeder et al Nat Methods. 2013 Feb 10. doi: 10.1038/n

Insert

TALE-activator p65 backbone

Use

Tale transcriptional activator expression vector

Tags

Expression

Mutation

Promoter

EF1alpha

Available since

March 8, 2013

Availability

Academic Institutions and Nonprofits only

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Kohinoor-Golgi/pcDNA3

Plasmid

#67779

Purpose

Golgi-targetted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Use

Tags

GT

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only
EGFP-mOrange pHis-//

Plasmid

#53302

Purpose

Expresses His-tagged EGFP-mOrange fusion protein.

Depositor

John Lis

Article

Tome et al Nat Methods. 2014 Jun;11(6):683-8. doi:

Insert

EGFP

Use

Tags

His and mOrange

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

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Kohinoor-Zyxin/pcDNA3

Plasmid

#67777

Purpose

Zyxin-targetted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Use

Tags

Zyxin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only
mRuby2-NT-N1

Plasmid

#54612

Purpose

Localization: N1 Cloning Vector, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Type

Empty backbone

Use

Tags

mRuby2-NT

Expression

Mammalian

Mutation

Promoter

Available since

June 22, 2015

Availability

Industry, Academic Institutions, and Nonprofits
MLM3583

Plasmid

#43887

Purpose

Activation Domain: VP64; RVD of C-terminal 0.5 TALE repeat: NG (binds T)

Depositor

Keith Joung

Article

Maeder et al Nat Methods. 2013 Feb 10. doi: 10.1038/n

Insert

TALE-activator VP64 Backbone

Use

Tale transcriptional activator expression vector

Tags

Expression

Mutation

Promoter

EF1alpha

Available since

March 8, 2013

Availability

Academic Institutions and Nonprofits only

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pPD540 Reporter Template v3

Plasmid

#138750

Purpose

Template for COMET promoters, expresses EYFP reporter upon promoter activation. BsaI sites produce non-palindromic sticky ends.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pDS255

Plasmid

#34970

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2(SS/TM)-GFP-LOVpep

Use

Tags

Expression

Yeast

Mutation

T406A, T407A

Promoter

Available since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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MLM2585

Plasmid

#43890

Purpose

Activation Domain: NF-kB; RVD of C-terminal 0.5 TALE repeat: NN (binds G)

Depositor

Keith Joung

Article

Maeder et al Nat Methods. 2013 Feb 10. doi: 10.1038/n

Insert

TALE-activator p65 backbone

Use

Tale transcriptional activator expression vector

Tags

Expression

Mutation

Promoter

EF1alpha

Available since

March 11, 2013

Availability

Academic Institutions and Nonprofits only

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pU6_(GLuc)_sgRNA

Plasmid

#68422

Purpose

Transient expression of a minimal sgRNA targeting the GLuc reporter, in mammalian cells. U6 promoter

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

Gluc sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pRATIO2251

Plasmid

#141305

Purpose

Gateway cloning compatible ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Use

Tags

Firefly Luciferase or LUC2 and mScarlet-I

Expression

Plant

Mutation

Promoter

UBQ10 promoter

Available since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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MLM3587

Plasmid

#43886

Purpose

Activation Domain: VP64; RVD of C-terminal 0.5 TALE repeat: NN (binds G)

Depositor

Keith Joung

Article

Maeder et al Nat Methods. 2013 Feb 10. doi: 10.1038/n

Insert

TALE-activator VP64 Backbone

Use

Tale transcriptional activator expression vector

Tags

Expression

Mutation

Promoter

EF1alpha

Available since

March 8, 2013

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNeo3/Cre

Plasmid

#89653

Purpose

Expresses a Neomycin-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting Neomycin

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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EE-SP!gIII

Plasmid

#48667

Purpose

Bacterial SP Cas9 targeting filamentous phage gene III at five protospacers

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Inserts

Cas9
anti-gIII tracrRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

proC and tracrRNA promoter

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pXN-FBLWLF-GAL80

Plasmid

#26265

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

GAL80 (GAL80 Budding Yeast)

Use

Drosophila transgenesis

Tags

Expression

Bacterial and Insect

Mutation

Promoter

Available since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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pDS247

Plasmid

#34967

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2(SS/TM)-GFP-LOVpep

Use

Tags

Expression

Yeast

Mutation

T406A, T407A, V416I, T–2S

Promoter

Available since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pDS248

Plasmid

#34968

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2(SS/TM)-GFP-LOVpep

Use

Tags

Expression

Yeast

Mutation

T406A, T407A, V416I

Promoter

Available since

March 21, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge