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We narrowed to 2,572 results for: GEM

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Showing: 481 - 520 of 2572 results

  1. pCRI001-pGEM-LS-Bar-PgpdA-dLbCas12a-VPR-Ttrpc-LS

    Plasmid
    #140193
    Purpose
    Chromosomal integration of PgpdA-dLbCas12a-VPR-TtrpC. Contains 1kb homology arms for Aspergillus nidulans and the bar gene for glufosinate selection. Filamentous fungi integrative vector.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Inserts
    dLbCas12a-VPR
    Bar
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
    Use
    CRISPR and Synthetic Biology; Fungal genome integ…
    Tags
    VPR (VP64-p65-Rta), NLS
    Mutation
    D832A DNAse deactivated
    Promoter
    PgpdA and PtrpC
    Available Since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    140193-plasmid-map-sequence-id-309648

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  2. pGEM BirA-2A-Citrine-SV40pA-FRT-Kan-FRT

    Plasmid
    #89890
    Purpose
    BAC donor construct containing 3XHA-tagged BirA, a ribosome skipping motif - 2A, Citrine reporter, polyadenyation signal, followed by FRT recombination sites flanking kanamycin selection cassette.
    Depositor
    Tatjana Sauka-Spengler
    Article
    Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d
    Insert
    HA-BirA-2A-citrine
    Use
    Unspecified
    Tags
    Citrine
    Available Since
    Aug. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    89890-plasmid-map-sequence-id-181309

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  12. pLXB3o1_hygR-MAR10-New BeYDV geminino-SP-nano72VHH-IgG1-MAR10 (GB4954)

    Plasmid
    #225441
    Purpose
    Module for stable transformation of Geminino 1.0-n72_h_HC flanked by two MAR10 insulators and next to a Hygromycin resistance cassette.
    Depositor
    Diego Orzaez
    Article
    Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1
    Insert
    hygR:MAR10:New BeYDV geminino:SP-nano72VHH-IgG1:MAR10
    Use
    Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available Since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    225441-plasmid-map-sequence-id-447713

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  14. pLV-hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110)-IRES-Blast

    Plasmid
    #193139
    Purpose
    Expresses bicistronic P2A construct of C-CRL4Cdt2 reporter(hCdt1(1-100)ΔCy-mCherry-) and APC/C reporter (hGeminin(1-110)) with blasticidin resistance
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110) (CDT1, GMNN Human)
    Use
    Lentiviral
    Mutation
    human Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…
    Promoter
    EF1a
    Available Since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193139-plasmid-map-sequence-id-380234

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  17. pAAVS1_TREtight-GEM2-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid
    #197061
    Purpose
    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM2-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)
    Depositor
    Julia Mahamid
    Article
    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.
    Inserts
    Encapsulin
    Adaptor Protein
    Use
    CRISPR
    Tags
    FRB and HaloTag
    Expression
    Mammalian
    Mutation
    Codon-optimised for human cell expression
    Available Since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    197061-plasmid-map-sequence-id-402602

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  18. pAAVS1_TREtight-FRB-GEM23-Halo-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid
    #197065
    Purpose
    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of FRB-GEM23-Halo and Adaptor Protein (FKBP-SNAP-vhhGFP4)
    Depositor
    Julia Mahamid
    Article
    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.
    Inserts
    I3-01
    Adaptor Protein
    Use
    CRISPR
    Tags
    FRB and HaloTag
    Expression
    Mammalian
    Mutation
    Codon-optimised for human cell expression
    Available Since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    197065-plasmid-map-sequence-id-402601

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  19. pAAVS1_TREtight-GEM22-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid
    #197064
    Purpose
    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM22-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)
    Depositor
    Julia Mahamid
    Article
    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.
    Inserts
    Encapsulin
    Adaptor Protein
    Use
    CRISPR
    Tags
    FRB and HaloTag
    Expression
    Mammalian
    Mutation
    Codon-optimised for human cell expression
    Available Since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    197064-plasmid-map-sequence-id-402605

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  20. pAAVS1_TREtight-GEM4-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid
    #197062
    Purpose
    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM4-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)
    Depositor
    Julia Mahamid
    Article
    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.
    Inserts
    Encapsulin
    Adaptor Protein
    Use
    CRISPR
    Tags
    FRB and HaloTag
    Expression
    Mammalian
    Mutation
    Codon-optimised for human cell expression
    Available Since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    197062-plasmid-map-sequence-id-402606

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  21. pAAVS1_TREtight-GEM7-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid
    #197063
    Purpose
    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM7-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)
    Depositor
    Julia Mahamid
    Article
    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.
    Inserts
    Encapsulin
    Adaptor Protein
    Use
    CRISPR
    Tags
    FRB and HaloTag
    Expression
    Mammalian
    Mutation
    Codon-optimised for human cell expression
    Available Since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    197063-plasmid-map-sequence-id-408409

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  22. pEN435 - pCAGGS-TagBFP-hGeminin-2A-mCherry-hCdt1-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt Tigre targeting

    Plasmid
    #92139
    Purpose
    Targeting vector to introduce a Fucci cell cyle reporter cassette mCherry(G1)/BFP(S/G2) at the mouse TIGRE acceptor locus using puromycin selection
    Depositor
    Benoit Bruneau
    Article
    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do
    Insert
    chromatin licensing and DNA replication factor 1 (GMNN, CDT1 Human)
    Use
    Mouse Targeting
    Expression
    Mammalian
    Available Since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    92139-plasmid-map-sequence-id-349059

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  23. TRE-pfv-Sapphire

    Plasmid
    #231957
    Purpose
    The plasmid containing TRE-pfv-Sapphire for the doxycycline-inducible expression of genetically encoded multimeric nanoparticles (GEM)
    Depositor
    Michael Shtutman
    Article
    Korunova et al bioRxiv [Preprint]. 2024 Nov 18:2024.11.
    Insert
    genetically encoded multimeric nanoparticles
    Use
    Lentiviral; Doxocycline inducible
    Expression
    Mammalian
    Promoter
    tight TRE promoter
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    231957-plasmid-map-sequence-id-462153

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  24. pAN1232

    Plasmid
    #127164
    Purpose
    Vector for integrating the controller (constitutive expression of GEM-degronSwitch and pZ3 expression of key)
    Depositor
    Hana El-Samad
    Article
    Ng et al Nature. 2019 Jul 24. pii: 10.1038/s41586
    Insert
    pRPL18B-GEM-degronSwitchA-tENO2-pZ3-keyA-mTurquoise2-NLS(SV40)-tTDH1
    Use
    Synthetic Biology
    Available Since
    Feb. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    127164-plasmid-map-sequence-id-250325

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  25. pUBC-Pfv-Sapphire

    Plasmid
    #203649
    Purpose
    Mammalian lentiviral vector for the expression of the Sapphire-tagged 40nm GEM under the UBC promoter.
    Depositor
    Liam Holt
    Article
    Shu T et et al bioRxiv 2022
    Insert
    PfV
    Use
    Lentiviral
    Tags
    Sapphire
    Available Since
    July 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    203649-plasmid-map-sequence-id-402479

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  26. TetCMV-Pfv-Sapphire

    Plasmid
    #203650
    Purpose
    Mammalian lentiviral vector for the expression of the Sapphire-tagged 40nm GEM under the doxycycline-inducible promoter.
    Depositor
    Liam Holt
    Article
    Shu T et et al bioRxiv 2022
    Insert
    PfV
    Use
    Lentiviral
    Tags
    Sapphire
    Available Since
    July 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    203650-plasmid-map-sequence-id-402480

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  27. pUBC-Pfv-Sapphire_PURO

    Plasmid
    #203651
    Purpose
    Mammalian lentiviral vector for the expression of the Sapphire-tagged 40nm GEM under the UBC promoter, with a puromycin selection marker.
    Depositor
    Liam Holt
    Article
    Shu T et et al bioRxiv 2022
    Insert
    PfV
    Use
    Lentiviral
    Tags
    Sapphire
    Available Since
    July 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    203651-plasmid-map-sequence-id-402481

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  31. pUBC-Pfv-Sapphire-IRES-H2B-mCherry

    Plasmid
    #203648
    Purpose
    Mammalian lentiviral vector for the expression of the Sapphire-tagged 40nm GEM under the UBC promoter, with a secondary translation initiation site for H2B-mCherry expression.
    Depositor
    Liam Holt
    Article
    Shu T et et al bioRxiv 2022
    Insert
    PfV
    Use
    Lentiviral
    Tags
    Sapphire-IRES-H2B-mCherry
    Available Since
    July 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    203648-plasmid-map-sequence-id-402478

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  33. GMIP

    Plasmid
    #32046
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    GMIP (GMIP Human)
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    amino acids 80-357 only
    Available Since
    Jan. 13, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  37. pUBC-popTag-QtE-mScarlet

    Plasmid
    #240101
    Purpose
    Mammalian lentiviral vector for expressing the mScarlet-tagged 50nm-GEMs, encoded by the encapsulin protein from Quasibacillus thermotolerans and fused to a PopZ tag.
    Depositor
    Liam Holt
    Article
    Xie et al Mol Cell. 2024 Jul 25;84(14):2698-2716.e
    Insert
    Encapsulin
    Use
    Lentiviral
    Tags
    mScarlet and popZ
    Expression
    Bacterial and Mammalian
    Promoter
    UBC promoter
    Available Since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    240101-plasmid-map-sequence-id-476613

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Showing: 481 - 520 of 2572 results