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Showing: 481 - 509 of 509 results

  1. pCK353

    Plasmid
    #129697
    Purpose
    For insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120015170
    Depositor
    John Heap
    Article
    Kelly et al Microorganisms. 2019 Aug 16;7(8). pii: m
    Inserts
    PrhaBAD
    yfp
    rhaS
    Terminator ECK120015170
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    129697-plasmid-map-sequence-id-258482

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  2. pCK355

    Plasmid
    #129699
    Purpose
    For insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + ilvBN terminator
    Depositor
    John Heap
    Article
    Kelly et al Microorganisms. 2019 Aug 16;7(8). pii: m
    Inserts
    PrhaBAD
    yfp
    rhaS
    ilvBN terminator
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    129699-plasmid-map-sequence-id-258506

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  3. Cas9-NAT

    Plasmid
    #64329
    Purpose
    Cas9 expression cassette carried by a yeast single-copy episome plasmid
    Depositor
    Yong-Su Jin
    Article
    Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):
    Insert
    Human-optimized S. pyogenes Cas9 with Clonnat marker gene expression cassette
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    64329-plasmid-map-sequence-id-227458

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  6. pBpaRS3tRNA

    Plasmid
    #197102
    Purpose
    To express the p-benzoylphenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into p-benzoylphenylalanine.
    Depositor
    Kensaku Sakamoto
    Article
    Sato et al Biochemistry. 2011 Jan 18;50(2):250-7. d
    Insert
    TyrRS variant, amber suppressor tRNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Gly32, Ser107, Thr158, Ser159, Arg286 (TyrRS)
    Promoter
    Available since
    June 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    197102-plasmid-map-sequence-id-388022

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  7. pPlacPbuCas13b-gRNA-T-MS2

    Plasmid
    #184839
    Purpose
    Expression of a single-spacer CRISPR array with spacer #1 targeting the MS2 phage genome and expression of PbuCas13b in bacteria.
    Depositor
    Chase Beisel
    Article
    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2
    Insert
    single-spacer CRISPR array with spacer #1 targeting the MS2 phage genome, PbuCas13b nuclease
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119 and lac promoter
    Available since
    Oct. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    184839-plasmid-map-sequence-id-373965

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  8. pCK314

    Plasmid
    #110545
    Purpose
    Modified (delta CRP-binding site) rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteria
    Depositor
    John Heap
    Article
    Kelly et al ACS Synth Biol. 2018 Apr 2. doi: 10.1021
    Inserts
    Modified PrhaBAD (CRP-binding sites removed)
    yfp
    rhaS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    110545-plasmid-map-sequence-id-212174

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  9. pPlacPbuCas13b-gRNA-T-deGFP

    Plasmid
    #184840
    Purpose
    Expression of a single-spacer CRISPR array with spacer #1 targeting the mRNA of deGFP and expression of PbuCas13b in bacteria.
    Depositor
    Chase Beisel
    Article
    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2
    Insert
    single-spacer CRISPR array with spacer #1 targeting the the mRNA of deGFP, PbuCas13b nuclease
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119 and lac promoter
    Available since
    Oct. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    184840-plasmid-map-sequence-id-373961

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  12. P2061

    Plasmid
    #186299
    Purpose
    Expresses LexA-HBD-B112 under the control of pAct1 and Cas9 under the control of LexA-HBD-B112+Beta-estradiol inducible promoter
    Depositor
    Joerg Stelling
    Article
    Azizoglu et al Curr Protoc. 2023 Jan;3(1):e647. doi: 10
    Inserts
    LexA-HBD-B112
    Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    2xLexop-minimalCyc1 and PACT1
    Available since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    186299-plasmid-map-sequence-id-367190

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  13. pPlacPbuCas13b-gRNA-NT

    Plasmid
    #184838
    Purpose
    Expression of single-spacer CRISPR array with a shortened non-targeting spacer for PbuCas13b and expression of PbuCas13b in bacteria.
    Depositor
    Chase Beisel
    Article
    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2
    Insert
    Co-expression of single-spacer CRISPR array with a nontargeting spacer for PbuCas13b and PbuCas13b nuclease in bacteria.
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119 and lac promoter
    Available since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    184838-plasmid-map-sequence-id-373981

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  20. pTUM4

    Plasmid
    #230905
    Purpose
    Plasmid encoding four periplasmic folding helper proteins to boost the secretion of functional heterologous proteins in E. coli
    Depositor
    Arne Skerra
    Article
    Schlapschy et al Protein Eng Des Sel. 2006 Aug;19(8):385-
    Inserts
    FkpA peptidyl-prolyl cis-trans isomerase FkpA (fkpA E. coli)
    SurA (surA E. coli)
    DsbA (dsbA E. coli)
    DsbC (dsbC E. coli)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    230905-plasmid-map-sequence-id-460506

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  21. pCK306

    Plasmid
    #110544
    Purpose
    rhaBAD, a rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteria.
    Depositor
    John Heap
    Article
    Kelly et al ACS Synth Biol. 2018 Apr 2. doi: 10.1021
    Inserts
    PrhaBAD
    yfp
    rhaS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    N/A, PrhaBAD, and kanR promoter (upstream of kanR…
    Available since
    June 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    110544-plasmid-map-sequence-id-212164

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  22. pBZCas13b

    Plasmid
    #89898
    Purpose
    Bacterial expression for bzCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.
    Depositor
    Feng Zhang
    Article
    Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.
    Insert
    Cas13b
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Lac
    Available since
    May 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    89898-plasmid-map-sequence-id-178222

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  23. pPbcas13b

    Plasmid
    #89906
    Purpose
    Bacterial expression for pbCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.
    Depositor
    Feng Zhang
    Article
    Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.
    Insert
    Cas13b
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Lac
    Available since
    May 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    89906-plasmid-map-sequence-id-178224

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  28. G30, Cdc20-mCherry-AID tagging

    Plasmid
    #130270
    Purpose
    Tagging the Saccharomyces cerevisiae Cdc20 gene with a mCherry and auxin-degron tag, via homologous recombination
    Depositor
    Matthias Heinemann
    Article
    Ozsezen et al Cell Syst. 2019 Oct 4. pii: S2405-4712(1
    Insert
    Cdc20/mCherry-AID(71-114)-tCYC1-NatMX (CDC20 Synthetic, Budding Yeast)
    Use
    Tags
    AID(71-114) and mCherry
    Expression
    Yeast
    Mutation
    Promoter
    Native promoter
    Available since
    Sept. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    130270-plasmid-map-sequence-id-255828

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  29. pDual

    Plasmid
    #207883
    Purpose
    Expresses thermosensitive variants of the homing endonucleases I-OnuI and I-GpeMI, each in a different open reading frame. Both were made thermosensitive by a fusion with the L212P VMA1 intein.
    Depositor
    David Edgell
    Article
    Foo et al Nucleic Acids Res. 2024 Feb 28;52(4):206
    Inserts
    Thermosensitive fusion of I-OnuI and the L212P VMA1 intein.
    Thermosensitive fusion of I-GpeMI and the L212P VMA1 intein.
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Leucine 212 changed to proline, leucine 434 in th…
    Promoter
    Available since
    Aug. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    207883-plasmid-map-sequence-id-422555

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Showing: 481 - 509 of 509 results