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Showing: 601 - 650 of 78333 results

  9. mCitrine-Talin-ABM-10

    Plasmid
    #56569
    Purpose
    Localization: Talin Actin Binding Domain, Excitation: 516, Emission: 529
    Depositor
    Michael Davidson
    Insert
    Talin ABM
    Use
    Tags
    mCitrine
    Expression
    Mammalian
    Mutation
    Actin Binding Module; I/LWEQ = 197 amino acids fr…
    Promoter
    CMV
    Available since
    Dec. 5, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. CeCollectrin-like pXOOM

    Plasmid
    #48704
    Purpose
    Encodes the Caenorhabditis elegans Collectrin homologue for expressing in Xenopus Oocytes of Mammalian cells
    Depositor
    Dmitri Boudko
    Insert
    NM_058858 (C50F2.7 Nematode)
    Use
    Xenopus oocyte expression
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    t7
    Available since
    Nov. 12, 2013
    Availability
    Academic Institutions and Nonprofits only

  12. Top7 Eb13 triple

    Plasmid
    #12475
    Depositor
    David Baker
    Insert
    Top7 Eb13 triple
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Eb13 triple. Top7 extended.
    Promoter
    Available since
    March 13, 2007
    Availability
    Academic Institutions and Nonprofits only

  13. Top7 Eb8_2 triple

    Plasmid
    #12476
    Depositor
    David Baker
    Insert
    Top7 Eb8_2 triple
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Eb8_2 triple. Top7 extended.
    Promoter
    Available since
    March 13, 2007
    Availability
    Academic Institutions and Nonprofits only

  16. TRIPZ-bioGEF-UBnc-PURO

    Plasmid
    #208044
    Purpose
    Enables inducible expression of low-affinity Avitag-UBnc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin
    Depositor
    James Sutherland, Rosa Barrio
    Article
    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:
    Insert
    UBnc (UBC Human)
    Use
    Lentiviral
    Tags
    Low-affinity Avitag
    Expression
    Mutation
    L73P Mutation near C-terminus suppresses cleavage…
    Promoter
    tetO/UBC
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208044-plasmid-map-sequence-id-410266

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  20. C1-mCitrine-FKBP-EZRabd(t567D)

    Plasmid
    #155227
    Purpose
    Acute increase in MPA when used with FRB-FKBP dimerizaton system upon addition with Rapamycin
    Depositor
    Tobias Meyer
    Article
    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210
    Insert
    mCitrine fused to FKBP and the actin BD of Ezrin (T567D), aa552-586 for use with FRB and rapamycin induced dimerization system
    Use
    Tags
    mCitrine
    Expression
    Mammalian
    Mutation
    Changed Thr 567 to Asp in original Ezrin
    Promoter
    Available since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    155227-plasmid-map-sequence-id-310009

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  22. TRUPATH Triple (bidirectional) GasS

    Plasmid
    #196055
    Purpose
    Encodes a G alpha subunit (GNAS2) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphasS-RLuc8
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196055-plasmid-map-sequence-id-385866

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  27. pCM66T: trimmed pCM66 backbone

    Plasmid
    #74738
    Purpose
    Small IncP-based broad host range replicating vector
    Depositor
    Mary Lidstrom
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    74738-plasmid-map-sequence-id-141453

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  30. pLVX-FLAG-mCherry-C1-centrin-1

    Plasmid
    #73333
    Purpose
    Lentiviral vector encoding centrin-1 with N-terminal FLAG and mCherry tags
    Depositor
    Manuel Thery
    Insert
    centrin-1 (CETN1 Human)
    Use
    Lentiviral
    Tags
    FLAG and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    73333-plasmid-map-sequence-id-139042

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  41. pTriEx-mCherry-PA-Rac1-C450A

    Plasmid
    #22028
    Purpose
    Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)
    Depositor
    Klaus Hahn
    Article
    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
    Insert
    PA-Rac1-C450A (RAC1 Avena sativa (oat), Human)
    Use
    Tags
    6xHis and mCherry
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    LOV(Leu404-Leu546, C450A). Rac1 starts at Ile4 an…
    Promoter
    CMV, p10
    Available since
    Sept. 29, 2009
    Availability
    Academic Institutions and Nonprofits only
    22028-plasmid-map-sequence-id-213358

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  44. pMSCV-IRES-mAmetrine 1.1 FP

    Plasmid
    #52113
    Purpose
    a bicistronic IRES-mAtetrine containing retroviral vector
    Depositor
    Dario Vignali
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    IRES-mAmetrine 1.1
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    52113-plasmid-map-sequence-id-80499

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Showing: 601 - 650 of 78333 results