We narrowed to 50,629 results for: ang

1. pLenti-CamkII-MacQ-mOrange2

   Plasmid

   #48761

   Purpose

   sensor

   Depositor

   Mark Schnitzer

   Article

   Gong et al Nat Commun. 2014 Apr 22;5:3674. doi: 10.

   Insert

   macQ-mOrange
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   mac-D139Q

   Promoter

   CamKIIa

   Available Since

   Jan. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Orange Nano-lantern/pcDNA3

   Plasmid

   #67661

   Purpose

   orange luminescent protein for high-speed single-cell and whole body imaging

   Depositor

   Takeharu Nagai

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   Orange Nano-lantern
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pSB700 lenti gRNA iRFP713_zhang2.0

   Plasmid

   #167916

   Purpose

   Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBactin-mOrange2-NEDD1-gTBD

   Plasmid

   #196858

   Purpose

   Expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange. Used to displace endogenous gamma-TuRC from the centrosome.

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   mOrange2-N-gTBD (NEDD1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available Since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pNH13 (pmyo-2::QuasAr::mOrange)

   Plasmid

   #130273

   Purpose

   Expresses the eFRET-based voltage sensor QuasAr::mOrange in the pharynx of C. elegans.

   Depositor

   Alexander Gottschalk

   Article

   Azimi Hashemi et al Proc Natl Acad Sci U S A. 2019 Aug 1. pi

   Insert

   QuasAr::mOrange
   

   Use

   Tags

   mOrange

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEB31 (hANGPTL8-V5-His lenti)

   Plasmid

   #109116

   Purpose

   V5 and His-tagged human ANGPTL8 in a lentiviral vector

   Depositor

   Brandon Davies

   Article

   Chi et al Mol Metab. 2017 Oct;6(10):1137-1149. doi

   Insert

   ANGPTL8 (ANGPTL8 Human)
   

   Use

   Lentiviral

   Tags

   6x His and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. FCK-QuasAr2-mOrange2 eFRET

   Plasmid

   #59173

   Purpose

   Fluorescent membrane voltage sensor

   Depositor

   Adam Cohen

   Article

   Zou et al Nat Commun. 2014 Aug 13;5:4625. doi: 10.

   Insert

   QuasAr2-mOrange2 eFRET
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFA6a-link-yoPSmOrange-Kan

   Plasmid

   #44951

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized photoconvertible mOrange with Kan selection

   Depositor

   Kurt Thorn, Wendell Lim

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoPSmOrange
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMTB2-Avi-Cerulean-RanGAP

   Plasmid

   #79878

   Purpose

   Beta-actin2 promoter driving N-term Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
   

   Use

   Tags

   Avi

   Expression

   Bacterial

   Mutation

   Promoter

   Beta-actin2

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Orange Nano-lantern/pRSETB

    Plasmid

    #67659

    Purpose

    orange luminescent protein for high-speed single-cell and whole body imaging

    Depositor

    Takeharu Nagai

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Orange Nano-lantern
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pSB1C3-J23110-B0034-mRFP1_Orange

    Plasmid

    #160444

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Orange chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

    Insert

    promoter, RBS, mRFP1E_Orange
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BioBrick sites removed

    Promoter

    Available Since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFA6a-link-yoPSmOrange-SpHis5

    Plasmid

    #44856

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized photoconvertible mOrange with SpHis5 selection

    Depositor

    Kurt Thorn, Wendell Lim

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoPSmOrange
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-hSyn-ChrimsonR-LSSmOrange

    Plasmid

    #149691

    Purpose

    For packaging and expression of ChrimsonR-LSSmOrange in neurons via AAV

    Depositor

    Robert Malenka

    Article

    Christoffel et al Proc Natl Acad Sci U S A. 2021 Jun 15;11

    Insert

    ChrimsonR
    

    Use

    AAV

    Tags

    LSSmOrange

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available Since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMTB2-RanGAP-Cerulean-Avi

    Plasmid

    #80068

    Purpose

    Beta-actin2 promoter driving C-term Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
    

    Use

    Zebrafish biotagging

    Tags

    Avi

    Expression

    Bacterial

    Mutation

    Promoter

    Beta-actin2

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAS1-tax-4P::his-24::mOrange

    Plasmid

    #124340

    Purpose

    Multicolor Worms for Neuronal ID

    Depositor

    Andrew Leifer

    Insert

    tax-4, mOrange (tax-4 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEB5 (mANGPTL8-V5-His lenti)

    Plasmid

    #109115

    Purpose

    V5 and His-tagged mouse ANGPTL8 in a lentiviral vector

    Depositor

    Brandon Davies

    Article

    Chi et al Mol Metab. 2017 Oct;6(10):1137-1149. doi

    Insert

    ANGPTL8 (Angptl8 Mouse)
    

    Use

    Lentiviral

    Tags

    6x His and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTRE3G-orangutan cGASdeltaN-GFP

    Plasmid

    #186846

    Purpose

    Doxycycline-dependent expression of orangutan cGAS gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

    Insert

    Orangutan cGAS truncation mutant (158-521 a.a.) with C-terminal GFP tag (CGAS Pongo abelii, Synthetic)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mutation

    N-terminal truncation

    Promoter

    TRE3G

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBactin-mOrange2-Cdk5rap2-CTD

    Plasmid

    #196855

    Purpose

    Expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to mOrange2. Used to displace endogenous Cdk5rap2 from centrosomes.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    mOrange2-Cdk5rap2-CTD (Cdk5rap2 Discosoma sp.)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None (wt)

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBactin-Nedd1-mOrange2-rCM1

    Plasmid

    #196861

    Purpose

    Expression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2 and rat (r) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Nedd1-mOrange2-rCM1 (Nedd1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBactin-Nedd1-mOrange2-F75A

    Plasmid

    #196862

    Purpose

    Expression of Need1-rCM1 harboring a (F75A) mutation in the rat (r) Centrosomin motif 1 (CM1) and fused to mOrange2. F75A mutatuon renders CM1 incapable of binding and activating gamma-TuRC

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Nedd1-mOrange2-F75A (Nedd1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F75A in the CM1 domain

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pORANGE_NFL linker(A6TAG)-3xFLAG KI

    Plasmid

    #182680

    Purpose

    Expression of spCas9, gRNA targeting the end of mouse Nefl gene and donor linker(A6TAG)-3xFLAG. Can be used for amber codon suppression and click chemistry labeling of endogenous NFL.

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Insert

    Nefl-targeting gRNA and linker(A6TAG)-3xFLAG donor sequence (Nefl Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and chicken beta-actin promoter

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSB700 lenti gRNA Hygro_zhang2.0

    Plasmid

    #167915

    Purpose

    Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSB700 lenti gRNA EYFP_zhang2.0

    Plasmid

    #167914

    Purpose

    Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. YIplac204-pOst1-ESCargo(Orange)

    Plasmid

    #140157

    Purpose

    Expresses a regulatable secretory cargo for yeast at the TRP1 locus

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    pOst1-APVNTT-E2-Orange-FKBP(LV)(C22V)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TPI1

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. YIplac128-pOst1-ESCargo(Orange)

    Plasmid

    #140158

    Purpose

    Expresses a regulatable secretory cargo for yeast at the LEU2 locus

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    pOst1-APVNTT-E2-Orange-FKBP(LV)(C22V)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TPI1

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAS1-rab-3P::his-24::mOrange

    Plasmid

    #124346

    Purpose

    Nuclear Localized Pan-Neuronal mOrange

    Depositor

    Andrew Leifer

    Insert

    rab-3, mOrange (rab-3 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pORANGE LOX Cloning template vector

    Plasmid

    #139651

    Purpose

    Cloning template for ORANGE-CAKE conditional knock-in constructs

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Orange Nano-lantern(Ca2+)/pcDNA3

    Plasmid

    #71346

    Purpose

    Express Orange Nano-lantern-based luminescent Ca2+ indicator in mammalian cell

    Depositor

    Takeharu Nagai

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Orange Nano-lantern(Ca2+)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

29. pEF-pIgH-ESCargo(FTV,Orange)

    Plasmid

    #140161

    Purpose

    Expresses a regulatable secretory cargo for mammalian cells

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    piGH-FTVNTT-E2-Orange-FKBP(LV)(C22V)
    

    Use

    Tags

    ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-hSyn-ChrimsonR-LSSmOrange-NRN

    Plasmid

    #149693

    Purpose

    For packaging and expression of ChrimsonR-LSSmOrange featuring a neuritin (NRN) 3' UTR in neurons via AAV

    Depositor

    Robert Malenka

    Article

    Christoffel et al Proc Natl Acad Sci U S A. 2021 Jun 15;11

    Insert

    ChrimsonR
    

    Use

    AAV

    Tags

    LSSmOrange

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available Since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pN_35S/mCitrine/P_UBQ10/Derlin1-mOrange2

    Plasmid

    #118000

    Purpose

    Co-expression of mCitrine under the control of the CaMV 35S promoter and Derlin1 fused to mOrange2 (an ER-targeting marker protein) under the control of the A. thaliana ubiquitin-10 promoter.

    Depositor

    Mathias Pribil

    Article

    Behrendorff et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

    Insert

    Derlin1-mOrange2
    

    Use

    Plant/bacteria binary vector

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Ubiquitin-10

    Available Since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMT-ubb-Avi-Cerulean-RanGAP

    Plasmid

    #79881

    Purpose

    Ubiquitin promoter driving Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
    

    Use

    Tags

    Avi

    Expression

    Bacterial

    Mutation

    Promoter

    Ubiquitin promoter

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLenti-CMV-orangutan cGASdeltaN-OMM

    Plasmid

    #186888

    Purpose

    Expression of orangutan cGAS gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

    Insert

    Orangutan cGAS truncation mutant (158-521 a.a.) with C-terminal MAVS transmembrane domain fusion (CGAS Pongo abelii, Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    N-terminal truncation, MAVS transmembrane domain …

    Promoter

    CMV

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMSCV-IRES-mOrange/CALR del52

    Plasmid

    #214768

    Purpose

    Mammalian expression of human CALR del52

    Depositor

    Norio Komatsu

    Article

    De Marchi et al Leuk Res. 2022 Aug;119:106883. doi: 10.1

    Insert

    human CALR del52 (CALR Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    MSCV

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMSCV-IRES-mOrange/CALR ins5

    Plasmid

    #214769

    Purpose

    Mammalian expression of human CALR ins5

    Depositor

    Norio Komatsu

    Article

    De Marchi et al Leuk Res. 2022 Aug;119:106883. doi: 10.1

    Insert

    human CALR ins5 (CALR Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    MSCV

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pJPT025-24 Angstrom 12TMD de novo_mRFP1_3x FLAG

    Plasmid

    #202507

    Purpose

    Express 24 Angstrom 12TMD de novo protein

    Depositor

    Neha Kamat

    Article

    Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

    Insert

    24 Angstrom 12TMD de novo
    

    Use

    Tags

    mRFP1-3x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pJPT026-32 Angstrom 4TMD de novo_mRFP1_3x FLAG

    Plasmid

    #202508

    Purpose

    Express 32 Angstrom 4TMD de novo protein

    Depositor

    Neha Kamat

    Article

    Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

    Insert

    32 Angstrom 4TMD de novo
    

    Use

    Tags

    mRFP1-3x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pJPT028-40 Angstrom 12TMD de novo_mRFP1_3x FLAG

    Plasmid

    #202510

    Purpose

    Express 40 Angstrom 12TMD de novo protein

    Depositor

    Neha Kamat

    Article

    Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

    Insert

    40 Angstrom 12TMD de novo
    

    Use

    Tags

    mRFP1-3x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pJPT024-24 Angstrom 4TMD de novo_mRFP1_3x FLAG

    Plasmid

    #202506

    Purpose

    Express 24 Angstrom 4TMD de novo protein

    Depositor

    Neha Kamat

    Article

    Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

    Insert

    24 Angstrom 4TMD de novo
    

    Use

    Tags

    mRFP1-3x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pJPT027-40 Angstrom 4TMD de novo_mRFP1_3x FLAG

    Plasmid

    #202509

    Purpose

    Express 40 Angstrom 4TMD de novo protein

    Depositor

    Neha Kamat

    Article

    Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

    Insert

    40 Angstrom 4TMD de novo
    

    Use

    Tags

    mRFP1-3x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pSB700 lenti gRNA KanR neo_zhang2.0

    Plasmid

    #167917

    Purpose

    Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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42. pFUGW ORANGE Gria1-GFP KI _ mCherry-KASH

    Plasmid

    #131507

    Purpose

    Endogenous tagging of GluA1: C-terminal (amino acid position: STOP codon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Gria1, Gria1 Mouse, Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hUBC

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCAG-T7-TALEN(Sangamo)-FokI-ELD-Destination

    Plasmid

    #40132

    Purpose

    Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (ELD).

    Depositor

    Pawel Pelczar

    Article

    Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791

    Type

    Empty backbone

    Use

    Tags

    FokI nuclease (ELD)

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Aug. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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44. pCAG-T7-TALEN(Sangamo)-FokI-KKR-Destination

    Plasmid

    #40131

    Purpose

    Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (KKR).

    Depositor

    Pawel Pelczar

    Article

    Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791

    Type

    Empty backbone

    Use

    Tags

    FokI nuclease (KKR)

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Aug. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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45. Optopatch2 (QuasAr2-mOrange2-P2A-CheRiff-eGFP)

    Plasmid

    #51656

    Purpose

    hsyn promoting optopatch, which includes a ChR64, GFP, Quasar2 and mOrange

    Depositor

    Adam Cohen

    Insert

    Quasar2-mOrange-Chr64-GFP
    

    Use

    Lentiviral

    Tags

    GFP and mOrange

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin

    Available Since

    March 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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46. pFUGW ORANGE Tubb3-GFP KI _ mCherry-KASH

    Plasmid

    #131508

    Purpose

    Endogenous tagging of β3 Tubulin: C-terminal (amino acid position: STOP codon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Tubb3, Tubb3 Mouse, Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hUBC

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. Nuc-Orange Nano-lantern(Ca2+)/pcDNA3

    Plasmid

    #71349

    Purpose

    Express Nuc-Orange Nano-lantern-based luminescent Ca2+ indicator in mammalian cel

    Depositor

    Takeharu Nagai

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Orange Nano-lantern(Ca2+)_H2B
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

48. pHDR-CDKN2A-Ex2-CMV-H2B-mOrange2

    Plasmid

    #110735

    Purpose

    Recombination template for replacing CDKN2A Exon2 with Ef1alpha-H2B-mOrange2

    Depositor

    Robert Judson-Torres

    Article

    Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

    Insert

    H2B-mOrange2
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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49. PB-H2A-LSSmOrange2-2A-ERK-KTR-LSS-SGFP2

    Plasmid

    #129629

    Purpose

    Visualization of ERK activity

    Depositor

    Dorus Gadella

    Insert

    PB-H2A-LSSmOrange2-2A-ERK-KTR-LSS-SGFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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50. pLenti-CMV-FLAG-orangutan cGASdeltaN-OMM

    Plasmid

    #186889

    Purpose

    Expression of orangutan cGAS gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

    Insert

    Orangutan cGAS truncation mutant (158-521 a.a.) with N-terminal FLAG tag and C-terminal MAVS transmembrane domain fusion (CGAS Pongo abelii, Synthetic)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    N-terminal truncation, MAVS transmembrane domain …

    Promoter

    CMV

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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