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We narrowed to 1,740 results for: plasmid for e coli

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Showing: 601 - 650 of 1740 results

  1. pCS2-MfnG-EcYRS-EGFP*

    Plasmid
    #191119
    Purpose
    A plasmid for incorporation of O-Me-Tyr into an EGFP reporter without O-Me-Tyr feeding for zebrafish
    Depositor
    Han Xiao
    Article
    Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:
    Insert
    MfnG - P2A - Mutant E. coli TyrRS - T2A - enhanced green fluorescence protein
    Tags
    His tag
    Expression
    Mammalian
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    191119-plasmid-map-sequence-id-373722

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  2. pET28-iLOVf

    Plasmid
    #63723
    Purpose
    E coli expression plasmid to produce Thrombin cleavable His tagged iLOV fusion proteins
    Depositor
    Brian Smith
    Type
    Empty backbone
    Tags
    TEE, iLOV, 6xHis, thrombin cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    63723-plasmid-map-sequence-id-110780

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  3. pET21a-LIC

    Plasmid
    #62303
    Purpose
    E. coli expression vector with N-terminal Hexa-HIS and TEV cleavage
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62303-plasmid-map-sequence-id-102191

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  4. Sc RFC5-pCDFDuet

    Plasmid
    #239202
    Purpose
    Overexpress Sc RFC5 in E. coli
    Depositor
    Michael O'Donnell
    Insert
    RFC5 (RFC5 Budding Yeast)
    Expression
    Bacterial
    Available Since
    June 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    239202-plasmid-map-sequence-id-474606

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  5. pOP5GP

    Plasmid
    #112610
    Purpose
    Used for expression of protein in E. coli as PreScission cleavable N-terminual 8His and GST tag fusions and with C-terminal Avi-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8His-GST and Avi
    Expression
    Bacterial
    Available Since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    112610-plasmid-map-sequence-id-220240

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  6. pOP5MP

    Plasmid
    #112612
    Purpose
    Used for expression of protein in E. coli as PreScission cleavable N-terminual 8His and MBP tag fusions and with C-terminal Avi-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8His-MBP and Avi
    Expression
    Bacterial
    Available Since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112612-plasmid-map-sequence-id-220250

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  7. pOP5GT

    Plasmid
    #112611
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and GST tag fusions and with C-terminal Avi-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8His-GST and Avi
    Expression
    Bacterial
    Available Since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112611-plasmid-map-sequence-id-220247

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  8. pOP5BT

    Plasmid
    #112609
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and GB1 tag fusions and with C-terminal Avi-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8His-GB1 and Avi
    Expression
    Bacterial
    Available Since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112609-plasmid-map-sequence-id-220239

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  9. pOP5TT

    Plasmid
    #112613
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and thioredoxin tag fusions and with C-terminal Avi-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8His-thioredoxin and Avi
    Expression
    Bacterial
    Available Since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112613-plasmid-map-sequence-id-220251

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  10. pOP5BP

    Plasmid
    #112608
    Purpose
    Used for expression of protein in E. coli as PreScission cleavable N-terminual 8His and GB1 tag fusions and with C-terminal Avi-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8His-GB1 and Avi
    Expression
    Bacterial
    Available Since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112608-plasmid-map-sequence-id-220237

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  12. pJMP3664

    Plasmid
    #222350
    Purpose
    Expression vector with PabstBR promoter, aprR marker, and replicates in A. baumannii and E. coli; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.
    Depositor
    Jason Peters
    Article
    Bacon et al Microbiol Spectr. 2024 Nov 5;12(11):e013
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    222350-plasmid-map-sequence-id-439070

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  13. pJMP3653

    Plasmid
    #222348
    Purpose
    Expression vector with PabstBR promoter, kanR marker, and replicates in A. baumannii and E. coli; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.
    Depositor
    Jason Peters
    Article
    Bacon et al Microbiol Spectr. 2024 Nov 5;12(11):e013
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    222348-plasmid-map-sequence-id-439082

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  14. pJMP3665

    Plasmid
    #222351
    Purpose
    Expression vector with PabstBR promoter, hygR marker, and replicates in A. baumannii and E. coli; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.
    Depositor
    Jason Peters
    Article
    Bacon et al Microbiol Spectr. 2024 Nov 5;12(11):e013
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    222351-plasmid-map-sequence-id-439073

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  17. pDP085

    Plasmid
    #198425
    Purpose
    pUBQ10:BirA. A pGreen based plasmid expressing E. coli BirA after the Arabidopsis pUBQ10 promoter. This plasmid is originated from You et al., Nat Commun. 2017, doi: 10.1038/ncomms15120
    Depositor
    Markus Schmid
    Article
    You et al Nat Commun. 2017 May 17;8:15120. doi: 10
    Insert
    E. coli BirA
    Expression
    Plant
    Promoter
    pUBQ10
    Available Since
    March 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    198425-plasmid-map-sequence-id-388239

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  19. pNIC28-Bsa4-iLOVf

    Plasmid
    #63722
    Purpose
    LIC E coli expression plasmid to produce TEV cleavable His tagged iLOV fusion proteins
    Depositor
    Brian Smith
    Type
    Empty backbone
    Tags
    TEE, iLOV, 6xHis, TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    63722-plasmid-map-sequence-id-106345

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  20. pTRC-LIC

    Plasmid
    #62343
    Purpose
    Used for tac promoter regulated high-level expression of cloned, untagged genes in E. Coli.
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    tac promoter from pKK233-2
    Available Since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62343-plasmid-map-sequence-id-102266

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  21. pFBOH-MHL

    Plasmid
    #62304
    Purpose
    It is a donor vector for generation of recombinant baculovirus by site-specific transposition in an E. coli host.
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Baculovirus expression
    Tags
    6xHis and TEV cleavage site
    Promoter
    polyhedrin
    Available Since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62304-plasmid-map-sequence-id-102204

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  22. p28BIOH-LIC

    Plasmid
    #62352
    Purpose
    E. coli expression vector with N-terminal AviTag and a C-terminal Hexa-HIS tag
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Tags
    6xHis and AviTag
    Expression
    Bacterial
    Promoter
    T7-lacO
    Available Since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62352-plasmid-map-sequence-id-102272

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  23. pGEX4T1-NDFIP2

    Plasmid
    #179330
    Purpose
    E. coli optimized expression of GST-fusion of human NDFIP2
    Depositor
    Arthur Haas
    Insert
    NDFIP2 (NDFIP2 Human)
    Tags
    GST
    Expression
    Bacterial
    Available Since
    April 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    179330-plasmid-map-sequence-id-358705

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  24. Sc Rad27-pET24

    Plasmid
    #239204
    Purpose
    Overexpress Sc Rad27 (FEN1) in E. coli
    Depositor
    Michael O'Donnell
    Insert
    Rad27 (RAD27 Budding Yeast)
    Expression
    Bacterial
    Available Since
    June 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    239204-plasmid-map-sequence-id-474607

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  25. pET/His-Rab1a(Q70L)

    Plasmid
    #232919
    Purpose
    E. coli Expression
    Depositor
    James Hurley
    Insert
    RAB1A (RAB1A Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Q70L
    Available Since
    March 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    232919-plasmid-map-sequence-id-465432

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  29. pECT3

    Plasmid
    #75450
    Purpose
    Plasmid that serves as the PCR template to create genomic pdt3 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate
    Depositor
    James Collins
    Article
    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.
    Insert
    pdt3
    Expression
    Bacterial
    Promoter
    none
    Available Since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75450-plasmid-map-sequence-id-146478

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  31. pECT5

    Plasmid
    #75451
    Purpose
    Plasmid that serves as the PCR template to create genomic pdt5 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate
    Depositor
    James Collins
    Article
    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.
    Insert
    pdt5
    Expression
    Bacterial
    Promoter
    none
    Available Since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75451-plasmid-map-sequence-id-146479

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  32. pECM1

    Plasmid
    #75445
    Purpose
    Plasmid that serves as the PCR template to create the genomic pdt1 insertions contained in the EPD library. Plasmid contains the R6K origin of replication so needs pir gene to replicate
    Depositor
    James Collins
    Article
    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.
    Insert
    pdt1
    Expression
    Bacterial
    Promoter
    none
    Available Since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75445-plasmid-map-sequence-id-146473

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  33. pECT1

    Plasmid
    #75446
    Purpose
    Plasmid that serves as the PCR template to create genomic pdt1 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate
    Depositor
    James Collins
    Article
    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.
    Insert
    pdt1
    Expression
    Bacterial
    Promoter
    none
    Available Since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75446-plasmid-map-sequence-id-146474

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  34. pECT1B

    Plasmid
    #75447
    Purpose
    Plasmid that serves as the PCR template to create genomic pdt1B insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate
    Depositor
    James Collins
    Article
    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.
    Insert
    pdt1B
    Expression
    Bacterial
    Promoter
    none
    Available Since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75447-plasmid-map-sequence-id-146475

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  35. pECT1D

    Plasmid
    #75448
    Purpose
    Plasmid that serves as the PCR template to create genomic pdt1D insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate
    Depositor
    James Collins
    Article
    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.
    Insert
    pdt1D
    Expression
    Bacterial
    Promoter
    none
    Available Since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75448-plasmid-map-sequence-id-146476

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  36. pECT1E

    Plasmid
    #75449
    Purpose
    Plasmid that serves as the PCR template to create genomic pdt1E insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate
    Depositor
    James Collins
    Article
    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.
    Insert
    pdt1E
    Expression
    Bacterial
    Promoter
    none
    Available Since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75449-plasmid-map-sequence-id-146477

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  37. pCas9cur

    Plasmid
    #71538
    Purpose
    Constitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.
    Depositor
    Tao Chen
    Article
    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10
    Inserts
    Cas9
    Plasmid curing system
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    pBAD
    Available Since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    71538-plasmid-map-sequence-id-129198

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  38. pREDCas9

    Plasmid
    #71541
    Purpose
    Constitutive expression of Cas9, inducible expression of the Red recombineering system, and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.
    Depositor
    Tao Chen
    Article
    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10
    Inserts
    Cas9
    lambda red genes
    plasmid curing system
    Expression
    Bacterial
    Promoter
    pBAD
    Available Since
    July 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71541-plasmid-map-sequence-id-333602

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  39. pCMV(MinDis).iGluSnFR

    Plasmid
    #41732
    Purpose
    Single-wavelength extracellular glutamate sensor constructed from E. coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:
    Insert
    GltI based Glutamate Biosensor
    Use
    Glutamate biosensor
    Tags
    Myc, PDGFR transmembrane helix (aa 513-561), and …
    Expression
    Mammalian
    Mutation
    cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…
    Promoter
    CMV
    Available Since
    Jan. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    41732-plasmid-map-sequence-id-210671

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  40. pET28a_SYK

    Plasmid
    #224240
    Purpose
    Expression of human SYK Kinase in E. coli
    Depositor
    Andrew Mesecar
    Insert
    syk (SYK Human)
    Tags
    6xHis and 6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7 promotor
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    224240-plasmid-map-sequence-id-459259

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  41. Sc FLAG-rad24-pRSFDuet

    Plasmid
    #240733
    Purpose
    Over-express FLAG-rad24 in E. coli
    Depositor
    Michael O'Donnell
    Insert
    FLAG-rad24 (RAD24 Budding Yeast)
    Tags
    3X FLAG
    Expression
    Bacterial
    Available Since
    Aug. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    240733-plasmid-map-sequence-id-482406

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  42. pET/His-GST-Rab1a(Q70L)

    Plasmid
    #232911
    Purpose
    E. coli Expression
    Depositor
    James Hurley
    Insert
    RAB1A (RAB1A Human)
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Q70L
    Available Since
    March 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    232911-plasmid-map-sequence-id-482325

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  43. pG2K

    Plasmid
    #71742
    Purpose
    Modular shuttle vector for Geobacillus and E. coli
    Depositor
    Tom Ellis
    Article
    Reeve et al ACS Synth Biol. 2016 Jul 1.
    Type
    Empty backbone
    Use
    Synthetic Biology; Shuttle vector
    Expression
    Bacterial
    Available Since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71742-plasmid-map-sequence-id-130147

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  44. pG1K

    Plasmid
    #71741
    Purpose
    Modular shuttle vector for Geobacillus and E. coli
    Depositor
    Tom Ellis
    Article
    Reeve et al ACS Synth Biol. 2016 Jul 1.
    Type
    Empty backbone
    Use
    Synthetic Biology; Shuttle vector
    Expression
    Bacterial
    Available Since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71741-plasmid-map-sequence-id-130144

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  45. pG1C

    Plasmid
    #71740
    Purpose
    Modular shuttle vector for Geobacillus and E. coli
    Depositor
    Tom Ellis
    Article
    Reeve et al ACS Synth Biol. 2016 Jul 1.
    Type
    Empty backbone
    Use
    Synthetic Biology; Shuttle vector
    Expression
    Bacterial
    Available Since
    July 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71740-plasmid-map-sequence-id-130141

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  46. pG1AK

    Plasmid
    #71736
    Purpose
    Modular shuttle vector for Geobacillus and E. coli
    Depositor
    Tom Ellis
    Article
    Reeve et al ACS Synth Biol. 2016 Jul 1.
    Type
    Empty backbone
    Use
    Synthetic Biology; Shuttle vector
    Expression
    Bacterial
    Available Since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71736-plasmid-map-sequence-id-130104

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  47. pDestTol2 ZfUbi::cMyc-oDam_f_GFPoNLS

    Plasmid
    #85821
    Purpose
    iDamID plasmid. To stably and ubiquitously express the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.
    Depositor
    Joachim Wittbrodt
    Article
    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261
    Insert
    Zf Ubi::cMyc oDam_f_GFPoNLS
    Use
    Tol2 based vector
    Tags
    cMyc N terminal to oDam_f_GFPoNLS and oNLS (optim…
    Mutation
    L122A. Codon optimization compatible to work in M…
    Available Since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    85821-plasmid-map-sequence-id-170479

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  50. pGRG36-Kn-PA1-GFP

    Plasmid
    #79088
    Purpose
    pGRG36-Kn-PA1-GFP is a Kanamycine resistance plasmid used to integrate a GFP expression cassette into the chromosome of E.coli, described in Yang et al. 2016 (Pending publication Pubmed ID)
    Depositor
    Frank Rosenzweig
    Article
    Yang et al Appl Environ Microbiol. 2020 Apr 1;86(8)
    Insert
    GFP
    Use
    Bacterial chromosome integration and expression o…
    Expression
    Bacterial
    Promoter
    PA1
    Available Since
    July 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    79088-plasmid-map-sequence-id-182383

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Showing: 601 - 650 of 1740 results