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We narrowed to 1,640 results for: plasmid for e coli

Showing: 601 - 650 of 1640 results

  1. pECT1D

    Plasmid
    #75448
    Purpose
    Plasmid that serves as the PCR template to create genomic pdt1D insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate
    Depositor
    James Collins
    Article
    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.
    Insert
    pdt1D
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    none
    Available since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75448-plasmid-map-sequence-id-146476

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  2. pECT1E

    Plasmid
    #75449
    Purpose
    Plasmid that serves as the PCR template to create genomic pdt1E insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate
    Depositor
    James Collins
    Article
    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.
    Insert
    pdt1E
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    none
    Available since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75449-plasmid-map-sequence-id-146477

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  3. pCas9cur

    Plasmid
    #71538
    Purpose
    Constitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.
    Depositor
    Tao Chen
    Article
    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10
    Inserts
    Cas9
    Plasmid curing system
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    71538-plasmid-map-sequence-id-129198

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  5. pCMV(MinDis).iGluSnFR

    Plasmid
    #41732
    Purpose
    Single-wavelength extracellular glutamate sensor constructed from E. coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:
    Insert
    GltI based Glutamate Biosensor
    Use
    Glutamate biosensor
    Tags
    Myc, PDGFR transmembrane helix (aa 513-561), and …
    Expression
    Mammalian
    Mutation
    cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…
    Promoter
    CMV
    Available since
    Jan. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    41732-plasmid-map-sequence-id-210671

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  6. pET28a_SYK

    Plasmid
    #224240
    Purpose
    Expression of human SYK Kinase in E. coli
    Depositor
    Andrew Mesecar
    Insert
    syk (SYK Human)
    Use
    Tags
    6xHis and 6xHis-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promotor
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    224240-plasmid-map-sequence-id-459259

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  7. pG2K

    Plasmid
    #71742
    Purpose
    Modular shuttle vector for Geobacillus and E. coli
    Depositor
    Tom Ellis
    Article
    Reeve et al ACS Synth Biol. 2016 Jul 1.
    Type
    Empty backbone
    Use
    Synthetic Biology; Shuttle vector
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71742-plasmid-map-sequence-id-130147

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  8. pG1K

    Plasmid
    #71741
    Purpose
    Modular shuttle vector for Geobacillus and E. coli
    Depositor
    Tom Ellis
    Article
    Reeve et al ACS Synth Biol. 2016 Jul 1.
    Type
    Empty backbone
    Use
    Synthetic Biology; Shuttle vector
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71741-plasmid-map-sequence-id-130144

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  9. pG1C

    Plasmid
    #71740
    Purpose
    Modular shuttle vector for Geobacillus and E. coli
    Depositor
    Tom Ellis
    Article
    Reeve et al ACS Synth Biol. 2016 Jul 1.
    Type
    Empty backbone
    Use
    Synthetic Biology; Shuttle vector
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71740-plasmid-map-sequence-id-130141

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  10. pG1AK

    Plasmid
    #71736
    Purpose
    Modular shuttle vector for Geobacillus and E. coli
    Depositor
    Tom Ellis
    Article
    Reeve et al ACS Synth Biol. 2016 Jul 1.
    Type
    Empty backbone
    Use
    Synthetic Biology; Shuttle vector
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71736-plasmid-map-sequence-id-130104

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  11. pDestTol2 ZfUbi::cMyc-oDam_f_GFPoNLS

    Plasmid
    #85821
    Purpose
    iDamID plasmid. To stably and ubiquitously express the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.
    Depositor
    Joachim Wittbrodt
    Article
    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261
    Insert
    Zf Ubi::cMyc oDam_f_GFPoNLS
    Use
    Tol2 based vector
    Tags
    cMyc N terminal to oDam_f_GFPoNLS and oNLS (optim…
    Expression
    Mutation
    L122A. Codon optimization compatible to work in M…
    Promoter
    Available since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    85821-plasmid-map-sequence-id-170479

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  15. pLVX-TetOne-Puro-EcSTH-FLAG

    Plasmid
    #218628
    Purpose
    This plasmid contains human codon optimized sequence of EcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.
    Depositor
    Valentin Cracan
    Article
    Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038
    Insert
    EcSTH
    Use
    Lentiviral and Synthetic Biology
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    218628-plasmid-map-sequence-id-432188

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  16. pLVX-TetOne-Puro-mitoEcSTH-FLAG

    Plasmid
    #218629
    Purpose
    This plasmid contains human codon optimized sequence of mitoEcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.
    Depositor
    Valentin Cracan
    Article
    Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038
    Insert
    mitoEcSTH
    Use
    Lentiviral and Synthetic Biology
    Tags
    FLAG and MTS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    218629-plasmid-map-sequence-id-432199

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  17. pGRG36-Kn-PA1-GFP

    Plasmid
    #79088
    Purpose
    pGRG36-Kn-PA1-GFP is a Kanamycine resistance plasmid used to integrate a GFP expression cassette into the chromosome of E.coli, described in Yang et al. 2016 (Pending publication Pubmed ID)
    Depositor
    Frank Rosenzweig
    Article
    Yang et al Appl Environ Microbiol. 2020 Apr 1;86(8)
    Insert
    GFP
    Use
    Bacterial chromosome integration and expression o…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PA1
    Available since
    July 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    79088-plasmid-map-sequence-id-182383

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  23. pGEX4T-1-Optimized UBE1L

    Plasmid
    #165099
    Purpose
    ecoli optimized expression of GST-fusion of human Ube1L
    Depositor
    Arthur Haas
    Insert
    UbeE1L (UBA7 Human)
    Use
    Tags
    GST from plasmid.
    Expression
    Bacterial
    Mutation
    codon optimized for E. coli expression
    Promoter
    Available since
    March 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    165099-plasmid-map-sequence-id-327741

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  24. pNIC28-Bsa4-TF

    Plasmid
    #61689
    Purpose
    E. coli expression vector based on pNIC28-Bsa4 (addgene #26103) with the TEE and trigger factor sequences inserted
    Depositor
    Brian Smith
    Type
    Empty backbone
    Use
    Tags
    HIS and Trigger Factor
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  25. Sc FLAG-S6-PCNA-pET

    Plasmid
    #239471
    Purpose
    Overexpress Sc FLAG-S6-PCNA in E. coli
    Depositor
    Michael O'Donnell
    Insert
    Sc FLAG-S6-PCNA
    Use
    Tags
    3X FLAG followed by S6
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    239471-plasmid-map-sequence-id-475695

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  26. pRF0G-Tyr

    Plasmid
    #113644
    Purpose
    Plasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging tyrosine at amber stop codons.
    Depositor
    Jeffrey Barrick
    Article
    Monk et al ACS Synth Biol. 2016 Sep 20.
    Insert
    MjYRS and tRNA gene
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 27, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    113644-plasmid-map-sequence-id-217129

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  27. pDEINO5

    Plasmid
    #179489
    Purpose
    Multi-host shuttle plasmid for E. coli, D. radiodurans, and S. cerevisiae; contains an oriT for transfer via conjugation
    Depositor
    Bogumil Karas
    Article
    Brumwell et al ACS Synth Biol. 2022 Mar 18;11(3):1068-1
    Insert
    aacC1
    Use
    Synthetic Biology; Bacterial and yeast cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    179489-plasmid-map-sequence-id-360441

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  28. pSAH0016

    Plasmid
    #79824
    Purpose
    Dual selection plasmid with lac operator, selection for binding with spectinomycin, for non-binding with chloramphenicol
    Depositor
    Katja Arndt
    Article
    Hoffmann et al Nucleic Acids Res. 2016 Jun 2;44(10):e95
    Inserts
    cat
    aadA
    Use
    Tags
    Expression
    Mutation
    Promoter
    AATTCAAAAAAATGCTTGACAATTAATCATCGGCTCGTATAATGTGTGG…
    Available since
    Aug. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    79824-plasmid-map-sequence-id-149510

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  29. pREDCas9

    Plasmid
    #71541
    Purpose
    Constitutive expression of Cas9, inducible expression of the Red recombineering system, and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.
    Depositor
    Tao Chen
    Article
    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10
    Inserts
    Cas9
    lambda red genes
    plasmid curing system
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    July 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71541-plasmid-map-sequence-id-333602

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  30. p46Cpf1-OP2

    Plasmid
    #98592
    Purpose
    Produces lambda Red and Cpf1 for recombination and selection. The plasmid is combined with a donor plasmid (with crRNA and template) for rapid genome editing in E.coli.
    Depositor
    Qiong Wu
    Article
    Ao et al Front Microbiol. 2018 Oct 9;9:2307. doi:
    Insert
    FnCpf1
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Codon optimized for E. coli
    Promoter
    araBAD
    Available since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    98592-plasmid-map-sequence-id-201329

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  31. pCVD419

    Plasmid
    #63754
    Purpose
    3.4 kb HindIII fragment from EHEC associated plasmid from E. coli 0157:H7 strain 933 cloned into pBR325. Contains the hlyA gene. Best EHEC probe for detection of this plasmid.
    Depositor
    James Kaper
    Article
    Levine et al J Infect Dis. 1987 Jul;156(1):175-82.
    Insert
    ehxA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 6, 2015
    Availability
    Academic Institutions and Nonprofits only

  32. COVID-SARS2 NSP14/10

    Plasmid
    #159613
    Purpose
    Bacterial co-expression vector fo Covid-SARS2 NSP14 and NSP10
    Depositor
    Opher Gileadi
    Article
    Baddock et al Nucleic Acids Res. 2022 Jan 17. pii: 650
    Inserts
    Covid-SARS2 Nsp14 (ORF1ab Synthetic, COVID-SARS2)
    Covid-SARS2 Nsp10
    Use
    Tags
    His6, TEV cleavage site and none
    Expression
    Bacterial
    Mutation
    Codon-optimized for E. coli expression
    Promoter
    (Bicistronic) and T7 - LacO
    Available since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    159613-plasmid-map-sequence-id-311056

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  33. pACYC-LIC+

    Plasmid
    #62312
    Purpose
    E. coli pACYC based expression vector with N-terminal Hexa-HIS and a thrombin protease cleavage site
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    6xHis and thrombin protease cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  34. pADsacA

    Plasmid
    #158649
    Purpose
    Constitutive transcription of sacA crRNA and GFP
    Depositor
    Wenjing Cui
    Insert
    sacA crRNA
    Use
    CRISPR and Synthetic Biology; Shuttle vector baci…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pveg
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158649-plasmid-map-sequence-id-310569

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  35. pHTganA-Cpf1

    Plasmid
    #158647
    Purpose
    Constitutive transcription of FnCpf1 and ganA crRNA
    Depositor
    Wenjing Cui
    Insert
    Cpf1, ganA crRNA
    Use
    CRISPR and Synthetic Biology; Shuttle vector baci…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P43
    Available since
    Oct. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    158647-plasmid-map-sequence-id-314144

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  36. pBsacA

    Plasmid
    #158650
    Purpose
    Constitutive transcription of sacA crRNA
    Depositor
    Wenjing Cui
    Insert
    sacA crRNA
    Use
    CRISPR and Synthetic Biology; Shuttle vector baci…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pveg
    Available since
    Oct. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    158650-plasmid-map-sequence-id-314145

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  37. pACYC-GST

    Plasmid
    #62329
    Purpose
    E. coli pACYC based expression vector with N-terminal TEV cleavable GST-tag
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    Glutathione S-transferase (GST) tag and TEV prote…
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lac0
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62329-plasmid-map-sequence-id-296399

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  38. pET32a-LIC

    Plasmid
    #62310
    Purpose
    E. coli expression vector with N-terminal Trx-tag, 6X His-tag, thrombin site, S-tag, and TEV
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    6xHis, S-tag, and Trx-Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62310-plasmid-map-sequence-id-326570

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  39. Sc Rad27-S6- pET24

    Plasmid
    #239205
    Purpose
    Overexpress Sc Rad27 (FEN1) (C-terminal S6 tag) in E. coli
    Depositor
    Michael O'Donnell
    Insert
    Rad27 (RAD27 Budding Yeast)
    Use
    Tags
    S6
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    239205-plasmid-map-sequence-id-474608

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  40. Sc FLAG-RFC1-pRSFDuet

    Plasmid
    #239201
    Purpose
    Overexpress Sc RFC1 (N-terminal 3X FLAG tag) in E. coli
    Depositor
    Michael O'Donnell
    Insert
    RFC1 (RFC1 Budding Yeast)
    Use
    Tags
    3X FLAG
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    239201-plasmid-map-sequence-id-474602

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  41. StarShip01

    Plasmid
    #164505
    Purpose
    To generate site specific attP-GA integrant cell lines upon co-delivery with BxbI. Contains T7-HaloTag-PDGFR_TM-T2A-Puro.
    Depositor
    Bo Huang
    Type
    Empty backbone
    Use
    Bxbi site specific integration
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    164505-plasmid-map-sequence-id-324722

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  42. StarShip02

    Plasmid
    #164506
    Purpose
    To generate site specific attP-GA integrant cell lines upon co-delivery with BxbI. Contains IRES(Myb)-T7-Halo-PDGFR_TM-T2A-Puro.
    Depositor
    Bo Huang
    Type
    Empty backbone
    Use
    Bxbi site specific integration
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    164506-plasmid-map-sequence-id-324723

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  43. StarShip03

    Plasmid
    #164507
    Purpose
    To generate site specific attP-GA integrant cell lines upon co-delivery with BxbI. Contains IRES2-T7-Halo-PDGFR_TM-T2A-Puro.
    Depositor
    Bo Huang
    Type
    Empty backbone
    Use
    Bxbi site specific integration
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    164507-plasmid-map-sequence-id-324724

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  44. pTuLys2CMR2

    Plasmid
    #53544
    Purpose
    Inhibition module. Constructed from the parent plasmids pLuxRI, pLuxmCherry, pET15bLCFPT7, and pLysS. The plasmid can express T7 lysozyme by inducing AHL and LuxR. Chloramphenicol resistance
    Depositor
    Lingchong You
    Article
    Payne et al Mol Syst Biol. 2013 Oct 8;9:697. doi: 10
    Insert
    pLux
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 26, 2015
    Availability
    Academic Institutions and Nonprofits only
    53544-plasmid-map-sequence-id-83437

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  45. pAcBac2.tR4-OMeYRS/GFP*

    Plasmid
    #50831
    Purpose
    baculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cells
    Depositor
    Peter Schultz
    Article
    Chatterjee et al Proc Natl Acad Sci U S A. 2013 Jul 16;11
    Inserts
    two-copy Tyr tRNA cassette
    EGFP*
    OMeYRS
    two copy Tyr tRNA cassette
    Use
    Baculoviral
    Tags
    His, Myc-6xHis, and WPRE
    Expression
    Insect
    Mutation
    Y39TAG and able to charge various unnatural amino…
    Promoter
    CAG, CMV, and U6 and H1
    Available since
    June 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    50831-plasmid-map-sequence-id-193569

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Showing: 601 - 650 of 1640 results