We narrowed to 4,337 results for: U6 gRNA

1. pAGK55

   Plasmid

   #228114

   Purpose

   Express node 2 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

   Depositor

   Seth Shipman

   Article

   Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

   Insert

   Retron node 2, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG for RT, U6/H1 for ncRNA/gRNA

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pML45

   Plasmid

   #206995

   Purpose

   Contains gRNA targeting mouse Ptbp1 gene and Cas9 ORF.

   Depositor

   Eugene Makeyev

   Article

   Kainov et al STAR Protoc. 2023 Oct 18;4(4):102644. do

   Insert

   Ptbp1 gRNA (Ptbp1 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUC19-sgNCF1

   Plasmid

   #190193

   Purpose

   Expresses a gRNA for base editing the Kozak sequence of the human NCF1 gene

   Depositor

   Alessandro Quattrone

   Article

   Ambrosini et al Nucleic Acids Res. 2022 Oct 14;50(18):10

   Insert

   gRNA sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. mCm7RNA6A

   Plasmid

   #84367

   Purpose

   Cas9-gRNA plasmid for mouse Cbfa2t2 m7 mutant knockin

   Depositor

   Danny Reinberg

   Article

   Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

   Insert

   Cbfa2t2 m7-gRNA6
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

5. pJA55

   Plasmid

   #59935

   Purpose

   gRNA for cleavage near sqt-1(e1350) locus in C elegans

   Depositor

   Andrew Fire

   Article

   Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

   Insert

   sqt-1(e1350) gRNA2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Sept. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PB-GG-OMKSL-PGK-Puro

   Plasmid

   #102894

   Purpose

   PiggyBac transposon system construct with 10 concatenated U6 promoter driven transcriptional cassettes targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters. Contains PGK-puro selection cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   OMKSL-gRNAs-PGK-Puro (POU5F1, MYC, KLF4, SOX2, LIN28A Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJW1328

   Plasmid

   #69488

   Purpose

   sgRNA(F+E) targeting pha-1 (no Cas9); for co-conversion in C. elegans

   Depositor

   Jordan Ward

   Insert

   sgRNA(F+E) targeting pha-1
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   R07E5.16 U6 promoter

   Available since

   Sept. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PB-2G-CNCB

   Plasmid

   #236764

   Purpose

   A piggybac-based cloning vector containing dual sgRNAs for spCas9 and CAG promoter-driven nuclear-localized Clover-T2A-BSR.

   Depositor

   Kazutoshi Takahashi

   Article

   Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do

   Type

   Empty backbone

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6 promoter, human U6 promoter, CAG promoter

   Available since

   May 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUS2-SH231

   Plasmid

   #115150

   Purpose

   Cas9-GFP, dual gRNA expression vector for targeted integration of repair templates into safe harbor 231 locus in chromosome 4

   Depositor

   Raymond Monnat, Michael Phelps

   Article

   Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

   Insert

   Dual SH231 gRNAs
   

   Use

   CRISPR

   Tags

   3xFLAG-SV40NLS, GFP, and Nucleoplasmin NLS

   Expression

   Mammalian

   Mutation

   Promoter

   U6, CBh

   Available since

   Oct. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. OA-1045C

    Plasmid

    #125005

    Purpose

    Expresses gRNAs targeting hid, eve, and wingless

    Depositor

    Omar Akbari

    Article

    Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

    Insert

    eve gRNA (eve Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6:3

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. OA-1045B

    Plasmid

    #125004

    Purpose

    Expresses gRNAs targeting hid, eve, and hedgehog

    Depositor

    Omar Akbari

    Article

    Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

    Insert

    eve gRNA (eve Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6:3

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. OA-1045E

    Plasmid

    #125007

    Purpose

    Expresses tRNA-flanked gRNAs targeting hid, eve, and hedgehog

    Depositor

    Omar Akbari

    Article

    Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

    Insert

    eve gRNA (eve Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6:3

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. OA-1045A

    Plasmid

    #125003

    Purpose

    Expresses gRNAs targeting hid and eve

    Depositor

    Omar Akbari

    Article

    Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

    Insert

    eve gRNA (eve Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6:3

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pU6-sgCXCR4-2

    Plasmid

    #46917

    Purpose

    Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting endogenous CXCR4 gene

    Depositor

    Stanley Qi, Jonathan Weissman

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Inserts

    sgCXCR4 -2 (CXCR4 Human)
    Puromycin resistance and mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pU6-sgCD71-2

    Plasmid

    #46918

    Purpose

    Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting endogenous CD71 gene

    Depositor

    Stanley Qi, Jonathan Weissman

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Inserts

    sgCD71 -2 (TFRC Human)
    Puromycin resistance and mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PB-UniSAM

    Plasmid

    #99866

    Purpose

    Encodes for Cas9-VP64, MS2-p65-HSF1, mCherry and for the gRNA 2.0

    Depositor

    Lesley Forrester

    Article

    Fidanza et al Sci Rep. 2017 Jul 25;7(1):6394. doi: 10.

    Insert

    UniSAM-mCherry + U6-gRNA2.0
    

    Use

    CRISPR and Synthetic Biology; Dcas9-sam activation

    Tags

    Expression

    Mammalian

    Mutation

    BbsI sites in CDS were ablated by consensus mutat…

    Promoter

    EF1a

    Available since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LentiCRISPRv2-ATG5

    Plasmid

    #99573

    Purpose

    Expresses Cas9 with sgRNA targeting Exon 7 of ATG5

    Depositor

    Edward Campbell

    Article

    Imam et al J Virol. 2016 Jan 13. pii: JVI.03033-15.

    Insert

    gRNA targeting ATG5 (ATG5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSpCas9(BB)-2A-Puro-C9orf72

    Plasmid

    #83439

    Purpose

    Expresses Cas9 and a gRNA targeting C9orf72

    Depositor

    Shawn Ferguson

    Article

    Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

    Insert

    gRNA targeting human C9orf72 (C9orf72 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDR348 FANCF

    Plasmid

    #47510

    Purpose

    human gRNA expression vector targeting FANCF

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-FANCF (FANCF Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pZLCv2-gD4Z4-3-3xFLAG-dCas9-HA-2xNLS

    Plasmid

    #106354

    Purpose

    CRISPR/Cas9 engineered chromatin immunoprecipitation of D4Z4 locus

    Depositor

    Stephen Tapscott

    Article

    Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

    Insert

    D4Z4 gRNA-3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge