We narrowed to 34,448 results for: CaS

1. pCYP25

   Plasmid

   #212858

   Purpose

   Expresses the Streptomyces sp. HB202 LRD-modifying cytochrome P450 enzyme

   Depositor

   Christopher Voigt

   Article

   Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

   Insert

   cytochrome P450
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PcymRC

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCYP45

   Plasmid

   #212878

   Purpose

   Expresses the Streptomyces sp. BK447 LRD-modifying cytochrome P450 enzyme

   Depositor

   Christopher Voigt

   Article

   Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

   Insert

   cytochrome P450
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PcymRC

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCYP38

   Plasmid

   #212871

   Purpose

   Expresses the Xanthomonas translucens pv. phlei. LRD-modifying cytochrome P450 enzyme

   Depositor

   Christopher Voigt

   Article

   Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

   Insert

   cytochrome P450
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PcymRC

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCYP07

   Plasmid

   #212840

   Purpose

   Expresses the Bradyrhizobium sp. Rc3b LRD-modifying cytochrome P450 enzyme

   Depositor

   Christopher Voigt

   Article

   Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

   Insert

   cytochrome P450
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PcymRC

   Available since

   Feb. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLRD20

   Plasmid

   #208271

   Purpose

   Production of 13R-manoyl oxide in E. coli MEV20

   Depositor

   Christopher Voigt

   Article

   Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

   Inserts

   tHMGR
   abCAS
   cfTPS3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   abCAS(D537A)

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLRD07

   Plasmid

   #208260

   Purpose

   Production of (16R)-ent-kauran-16-ol in E. coli MEV20

   Depositor

   Christopher Voigt

   Article

   Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

   Inserts

   tHMGR
   ptmT2
   ptmT3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. C-SWAT 6FLAG

   Plasmid

   #182509

   Purpose

   C-SWAT compatible vector for C-terminal tagging with 6 FLAG

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   6 FLAG
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. C-SWAT GFP

   Plasmid

   #182510

   Purpose

   C-SWAT compatible vector for C-terminal tagging with GFP

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   GFP
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJH572

   Plasmid

   #172156

   Purpose

   Negative control pBAD backbone

   Depositor

   Matthew Bennett

   Article

   Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEMF038

    Plasmid

    #157635

    Purpose

    Empty vector

    Depositor

    Jonathan Silberg

    Article

    Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pNT-15

    Plasmid

    #154932

    Purpose

    15 designed non-target sites

    Depositor

    Michael Lynch

    Article

    Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

    Insert

    15 designed non-target sites
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNT-12

    Plasmid

    #154933

    Purpose

    12 designed non-target sites

    Depositor

    Michael Lynch

    Article

    Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

    Insert

    12 designed non-target sites
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pNT-3

    Plasmid

    #154934

    Purpose

    3 designed non-target sites

    Depositor

    Michael Lynch

    Article

    Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

    Insert

    3 designed non-target sites
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pNT-1

    Plasmid

    #154935

    Purpose

    1 designed non-target site

    Depositor

    Michael Lynch

    Article

    Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

    Insert

    1 designed non-target sites
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJZC530

    Plasmid

    #135434

    Purpose

    Yeast integrating plasmid for pTETO1-Venus reporter gene

    Depositor

    Jesse Zalatan

    Article

    Cunningham-Bryant et al Chembiochem. 2019 Jun 14;20(12):1519-152

    Insert

    pTETO1_Venus
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pTETO1

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJS032

    Plasmid

    #135431

    Purpose

    Yeast integrating plasmid to express PYL1-VP64

    Depositor

    Jesse Zalatan

    Article

    Cunningham-Bryant et al Chembiochem. 2019 Jun 14;20(12):1519-152

    Insert

    PYL1-VP64
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pAdh

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJS033

    Plasmid

    #135432

    Purpose

    Yeast integrating plasmid to express MCP-ABI

    Depositor

    Jesse Zalatan

    Article

    Cunningham-Bryant et al Chembiochem. 2019 Jun 14;20(12):1519-152

    Insert

    MCP-ABI
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pTEF1

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNX3a-PK9

    Plasmid

    #78356

    Purpose

    fission yeast epitope tagging

    Depositor

    Kazunori Tomita

    Article

    Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

    Inserts

    9xPK
    kanMX6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNXRVg-HACre

    Plasmid

    #78405

    Purpose

    constitutive Cre expression vector

    Depositor

    Kazunori Tomita

    Article

    Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

    Inserts

    3xHA-nls-Cre
    arg3+ (arg3 Fission Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNXRVh-HACre

    Plasmid

    #78406

    Purpose

    constitutive Cre expression vector

    Depositor

    Kazunori Tomita

    Article

    Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

    Inserts

    3xHA-nls-Cre
    leu1+ (leu1 Fission Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pNXRVk-HACre

    Plasmid

    #78408

    Purpose

    constitutive Cre expression vector

    Depositor

    Kazunori Tomita

    Article

    Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

    Inserts

    3xHA-nls-Cre
    ade6+ (ade6 Fission Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pNX3d-PK3

    Plasmid

    #78375

    Purpose

    fission yeast epitope tagging

    Depositor

    Kazunori Tomita

    Article

    Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

    Inserts

    3xPK
    ura4+ (ura4 Fission Yeast)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pNX3d-PK6

    Plasmid

    #78376

    Purpose

    fission yeast epitope tagging

    Depositor

    Kazunori Tomita

    Article

    Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

    Inserts

    6xPK
    ura4+ (ura4 Fission Yeast)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pNX3d-PK9

    Plasmid

    #78377

    Purpose

    fission yeast epitope tagging

    Depositor

    Kazunori Tomita

    Article

    Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

    Inserts

    9xPK
    ura4+ (ura4 Fission Yeast)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pNX3d-PK12

    Plasmid

    #78378

    Purpose

    fission yeast epitope tagging

    Depositor

    Kazunori Tomita

    Article

    Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

    Inserts

    12xPK
    ura4+ (ura4 Fission Yeast)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pNX3a-HA3

    Plasmid

    #78359

    Purpose

    fission yeast epitope tagging

    Depositor

    Kazunori Tomita

    Article

    Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

    Inserts

    3xHA
    kanMX6
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAKW.031-GM-R5

    Plasmid

    #154044

    Purpose

    Expresses R5 peptide in E. coli

    Depositor

    Christopher Voigt

    Article

    Wallace et al Advanced Functional Materials 2020

    Insert

    Silaffin R5 peptide
    

    Use

    Tags

    Maltose binding protein and his tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLG173

    Plasmid

    #124368

    Purpose

    Control plasmid with only spectinomycin

    Depositor

    Christopher Voigt

    Article

    Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

    Insert

    empty backbone (control) (NEWENTRY Synthetic)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    No promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLG320

    Plasmid

    #124370

    Purpose

    Vanillic acid inducible system for expression in B. subtilis

    Depositor

    Christopher Voigt

    Article

    Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

    Insert

    gfpmut2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pspank(V)

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. 6-His-MBP-TEV-FnCpf1

    Plasmid

    #90094

    Purpose

    Bacterial expression plasmid for protein purification

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    FnCpf1 (humanized)
    

    Use

    Tags

    3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. AAV-CMV-SOD2-2A-Catalase-WPRE

    Plasmid

    #67635

    Purpose

    AAV vector expressing both SOD2 and mitochondrial targeted Catalase

    Depositor

    Connie Cepko

    Article

    Xiong et al J Clin Invest. 2015 Apr;125(4):1433-45.

    Insert

    SOD-2A-Catalase (Sod2, Cat Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Deleted the Peroxisome Targeting Signal from Cata…

    Promoter

    CMV

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLenti_ABE8e-SpRY-P2A-BFP_HygroR

    Plasmid

    #213009

    Purpose

    A lentiviral vector used to create stable cell lines expressing the ABE8e-SpRY base editor and BFP

    Depositor

    Richard Sherwood

    Article

    Ryu et al Nat Genet. 2024 Apr 24. doi: 10.1038/s41

    Insert

    ABE8e-SpRY-D10A
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A nickase variant of SpRY

    Promoter

    Ef-1a

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDY1623 NOLC1 sgRNA 2

    Plasmid

    #234834

    Purpose

    sgRNA 2 for NOLC1 STITCHR insertion

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

    Insert

    NOLC1 sgRNA (NOLC1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pDY1622 NOLC1 sgRNA 1

    Plasmid

    #234833

    Purpose

    sgRNA 1 for NOLC1 STITCHR insertion

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

    Insert

    NOLC1 sgRNA (NOLC1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. lentiCRISPRv2FE-ABE8e-SpRY

    Plasmid

    #213008

    Purpose

    A lentiviral vector expressing the ABE8e-SpRY base editor and an sgRNA cloning site

    Depositor

    Richard Sherwood

    Article

    Ryu et al Nat Genet. 2024 Apr 24. doi: 10.1038/s41

    Insert

    ABE8e-SpRY-D10A
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A nickase variant of SpRY

    Promoter

    Ef-1a

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. AAV-U6-sgRNA-hSyn-mCherry

    Plasmid

    #87916

    Purpose

    sgRNA expressing AAV construct with a mCherry reporter driven by hSyn promoter. (replaced the GFP in pX552 from Zhang lab with mCherry)

    Depositor

    Alex Hewitt

    Article

    Hung et al Invest Ophthalmol Vis Sci. 2016 Jun 1;57

    Insert

    mCherry
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pVD1 tRNA-gRNA position E2 (GB2239)

    Plasmid

    #160561

    Purpose

    tRNA and scaffold for the assembly of GBoligomers for position [2-3] of a polycistronic tRNA-gRNA

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

    Insert

    tRNA-gRNA position E2 (Multiplexing Edit)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCAT077

    Plasmid

    #180511

    Purpose

    Plasmid expressing mammalian codon optimized wt PlmCasX, sgRNAv2 scaffold, and restriction sites to clone in new spacers

    Depositor

    Jennifer Doudna

    Article

    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

    Inserts

    CasX sgRNAv2
    PlmCasX
    

    Use

    CRISPR

    Tags

    2x FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and U6

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. PEmaxNG-_2star_IVT

    Plasmid

    #227678

    Purpose

    plasmid for SpCas9-NG-PEmax** mRNA IVT

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

    Insert

    NG-PEmax_2_star
    

    Use

    CRISPR

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAGT9054

    Plasmid

    #219430

    Purpose

    Transcriptional unit (MoClo-position 2): 2x35Ss_Omega enhancer_PapE-4LF2-N-SpCas9i-N_tOCS

    Depositor

    Tom Schreiber

    Article

    Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000

    Insert

    LB_2x35Ss-omega enhancer:PapE-4xLF2-NLS-SpCas9i-NLS:tOCS_RB (Position 2)
    

    Use

    CRISPR; Moclo compatible level 1 module (position…

    Tags

    PapE-4LF2

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCAT100

    Plasmid

    #180512

    Purpose

    Plasmid expressing mammalian codon optimized engineered chimeric PlmCasX-R1, sgRNAv2 scaffold, and restriction sites to clone in new spacers

    Depositor

    Jennifer Doudna

    Article

    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

    Inserts

    CasX sgRNAv2
    PlmCasX with DpbCasX R1 loop
    

    Use

    CRISPR

    Tags

    2x FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and U6

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCAT527

    Plasmid

    #180514

    Purpose

    Plasmid expressing mammalian codon optimized engineered chimeric PlmCasX-R1, mNeonGreen, sgRNAv2 scaffold, and restriction sites to clone in new spacers

    Depositor

    Jennifer Doudna

    Article

    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

    Inserts

    CasX sgRNAv2
    PlmCasX with DpbCasX R1 loop-2A-mNeonGreen
    

    Use

    CRISPR

    Tags

    2x FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and U6

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. SPACE (pRZ1813)

    Plasmid

    #140242

    Purpose

    CMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-UGI-UGI-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    SPACE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    V82G in TadA*, D10A in Cas9, R187W in pmCDA1

    Promoter

    CMV

    Available since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pAGT9569

    Plasmid

    #219431

    Purpose

    Transcriptional unit (MoClo-position 2): pAtRPS5a_PapE-4LF2-N-SpCas9i-N_tNOS

    Depositor

    Tom Schreiber

    Article

    Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000

    Insert

    LB_AtRPS5a:PapE-4xLF2-NLS-SpCas9i-NLS:tNOS_RB (Position 2)
    

    Use

    CRISPR and Synthetic Biology; Moclo compatible le…

    Tags

    PapE-4LF2

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. SPACE-NG (pRZ5150)

    Plasmid

    #140244

    Purpose

    CMV promoter expression plasmid for TadA*(V82G)-nCas9_NG-pmCDA1(R187W)-UGI-UGI-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    SPACE_NG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    V82G in TadA*, NG mutations in SpCas9, D10A in Sp…

    Promoter

    CMV

    Available since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pJEP13-AAV-H1/TO-L-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid

    #82703

    Purpose

    AAV backbone with a full length H1/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.

    Depositor

    Jonathan Ploski

    Article

    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do

    Insert

    H1/TO-L gRNA Expression Cassette Containing Tet2 gRNA
    

    Use

    AAV and CRISPR

    Tags

    CMV promoter driving expression of TetR-P2A-GFP-K…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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47. SECURE BE3(R33A/K34A)-P2A-EGFP (pJUL1410)

    Plasmid

    #123615

    Purpose

    CAG promoter expression plasmid for rAPOBEC1(R33A/K34A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A/K34A).

    Depositor

    Keith Joung

    Article

    Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

    Insert

    BE3(R33A/K34A)-P2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R33A/K34A in rAPOBEC1, D10A in SpCas9

    Promoter

    CAG

    Available since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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48. SECURE BE3(R33A)-P2A-EGFP (pJUL1085)

    Plasmid

    #123614

    Purpose

    CAG promoter expression plasmid for rAPOBEC1(R33A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A).

    Depositor

    Keith Joung

    Article

    Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

    Insert

    BE3(R33A)-P2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, D10A in SpCas9

    Promoter

    CAG

    Available since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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49. pCAT105

    Plasmid

    #180510

    Purpose

    Plasmid expressing mammalian codon optimized engineered DpbCasX-R3, sgRNAv2 scaffold, and restriction sites to clone in new spacers

    Depositor

    Jennifer Doudna

    Article

    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

    Inserts

    CasX sgRNAv2
    DpbCasX with R3 loop
    

    Use

    CRISPR

    Tags

    2x FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and U6

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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50. Lenti-BaeI-Pgk-Cre

    Plasmid

    #173663

    Purpose

    Vector with BaeI site to allow cloning of custom sgRNA into vector containing Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    BaeI site
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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