We narrowed to 8,173 results for: 11
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Plasmid#80591PurposeExpresses GFP from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.DepositorInsertGFP
UseAAVExpressionMammalianPromoterCBAAvailable SinceSept. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-Bsa4-ICL1
Plasmid#105822PurposeExpression of Mycobacterium tuberculosis icl1 recombinant proteinDepositorInserticl1
TagsHis tag with TEV cleavageExpressionBacterialPromoterT7Available SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
hisSUMO-Gli1
Plasmid#87225PurposeExpress hisSUMO tagged DNA binding domain of Gli1(mouse) in E. coliDepositorAvailable SinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
hisSUMO-ZFP57
Plasmid#102716PurposeExpress DNA binding domain of human Zinc finger protein 57 in E. coliDepositorAvailable SinceNov. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
dTomato_LD 0_CS M_tTA-BFP
Plasmid#58875PurposeMESA target chain with dTomato ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with dTomato ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1')
UseAAVTagsEBFPExpressionMammalianPromoterCMVAvailable SinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
CD4_LD 0_CS G_tTA-BFP
Plasmid#58869PurposeMESA target chain with CD4 ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with CD4 ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
UseAAVTagsEBFPExpressionMammalianPromoterCMVAvailable SinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
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Rix-PGK-Tom-W
Plasmid#25813DepositorInsertRix-PGK-Tom-W
UseLentiviralMutationtdTomato from Tsien labAvailable SinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-EphB2
Plasmid#200982PurposeMammalian expression plasmid for myc-tagged EphB2DepositorInsertEphB2 (EPHB2 Human)
TagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianPromoterCMVAvailable SinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
nLuc-PTHR1
Plasmid#162577PurposeExpresses N-terminally NanoLuc-Tagged PTHR1 in mammalian cellsDepositorAvailable SinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-LwaCas13acrRNA-BsmBIcassette (LTH151)
Plasmid#171129PurposeLwaCas13a crRNA entry vector for pU6 expression (need to clone in spacer into BsmBI sites)DepositorInsertU6 promoter entry plasmid for LwaCas13a crRNA cloning
UseCRISPRExpressionMammalianPromoterU6Available SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbB8k-csg-amylase
Plasmid#166859PurposeArabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to α-amylase via a flexible linker. Derived from https://www.addgene.org/35363.DepositorInsertcsgBACEFG, with α-amylase fused to CsgA via flexible linker
UseSynthetic BiologyExpressionBacterialAvailable SinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJYScr
Plasmid#132719PurposePlasmid for an easy-to-construct crRNA delivery vector using hybridized oligonucleotides. Constitutive transcription of crRNA, Spectinomycin resistanceDepositorTypeEmpty backboneUseCRISPRExpressionBacterialAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_WTDHFR_WTTS
Plasmid#91226Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
Tags6xHisExpressionBacterialPromoterT7Available SinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONRPEX18Km-SceI-pheS
Plasmid#111610PurposeGateway compatible allelic exchange vector for Burkholderia sp.DepositorTypeEmpty backboneUseAllelic exchange in burkholderia sp.Available SinceAug. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hARdelta120-472
Plasmid#89088Purposemammalian expression of human androgen receptor with deletion of 120-472 amino acid residuesDepositorInserthAR-120-472 N-term deletion (AR Human)
ExpressionMammalianMutationhAR-120-472PromoterCMVAvailable SinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLpC-Zipp.-L-IFP-F[2]+Venus (mammalian)
Plasmid#52906PurposeBinary retroviral pl. (puromycin). Zipper (PacI/ClaI):Linker (GGGGS)X2+IFP-F[2]. In the second MCS, the Venus fluorescent protein is cloned in between PmeI/SalI.Confers ampicillin resistance to DH5α.DepositorInsertZipp.-L-IFP-F[2]+Venus
UseRetroviralTagsL-IFP-F[2]ExpressionMammalianPromoterUnknownAvailable SinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
TALEN-CCR5R18-CtermQ7
Plasmid#51443PurposeExpresses TALEN targeting right CCR5A site (R18) with Q7 C-terminal domainDepositorInsertTALEN CCR5 Right (R18) Q7 C-term
UseTALENTags3xFLAG and Fok1 KK variantExpressionMammalianMutationK777Q, K778Q, K788Q, R789Q, R792Q, R793Q and R801QPromoterCMVAvailable SinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
FH1-CLGT#2-mCherry-_PGK-H2B-CFP
Plasmid#164106PurposeFluorescent reporter for genetic tracing of classical Glioblastoma cell-state.DepositorInsertCLGT#2-mCherry
UseLentiviral and Synthetic BiologyAvailable SinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xEcoLeuT(CUA)_AnapRS
Plasmid#140019PurposePlasmid with 4xEcoLeuT(CUA) cassette and E. coli AnapRS for amber suppression and incorporation of the fluorescent ncAA Anap; for transient or stable piggyBac-mediated integrationDepositorInsertEcoLeuRS (AnapRS)
ExpressionMammalianMutationL38F, M40G, L41P, Y499V, Y500L, Y527A, H537E, L53…PromoterEF1Available SinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only