We narrowed to 165,666 results for: addgene

1. pCMV-T7-ABE8e-nSpCas9-VRQR-P2A-EGFP (KAC1309)

   Plasmid

   #185913

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized ABE8e-nSpCas9-VRQR-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R

   Promoter

   CMV and T7

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJR152

   Plasmid

   #196280

   Purpose

   Cassette used in dual sgRNA library generation

   Depositor

   Rebecca Voorhees

   Article

   Guna et al BMC Genomics. 2023 Oct 30;24(1):651. doi

   Insert

   hU6-sgRNA-CR3
   

   Use

   CRISPR and Lentiviral

   Promoter

   hU6

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. CpxR-GFP

   Plasmid

   #220150

   Purpose

   To track the natural CpxR promoter's transcriptional dynamics

   Depositor

   Andre Sanches Ribeiro

   Article

   Dash et al mSystems. 2024 Jun 18;9(6):e0006524. doi

   Insert

   CpxR promoter only
   

   Expression

   Bacterial

   Available Since

   Aug. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pACAGW-oChiEFtdTomato-AAV

   Plasmid

   #32846

   Purpose

   AAV expression of mammalian codon optimized ChIEF for optogenetic excitation.

   Depositor

   Rory McQuiston

   Article

   Jurgens et al PLoS One. 2012;7(9):e45717. doi: 10.1371

   Insert

   oChiEF
   

   Use

   AAV

   Tags

   tdTomato

   Promoter

   CAGG

   Available Since

   March 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-Fab_8D3_2_H-H6

   Plasmid

   #176075

   Purpose

   Express His6 tagged heavy chain of Fab_8D3_2 in mammalian cells

   Depositor

   Tom Rapoport

   Article

   Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

   Insert

   His6 tagged heavy chain of Fab_8D3_2
   

   Tags

   His tag

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   Oct. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-EFS-SpCas9

   Plasmid

   #104588

   Purpose

   AAV vector expressing Myc-tagged SpCas9 under the EFS promoter

   Depositor

   Ryohei Yasuda

   Article

   Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)

   Insert

   Streptococcus pyogenes Cas9
   

   Use

   AAV and CRISPR

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEM184_30x147bp_Widom601

   Plasmid

   #240634

   Purpose

   30x 147-bp Widom 601 nucleosome positioning sequence

   Depositor

   Emma Morrison

   Article

   Hammonds et al bioRxiv [Preprint]. 2025 Apr 10:2025.04.

   Insert

   30x 147-bp Widom 601
   

   Use

   Dna amplification

   Available Since

   Sept. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIVM_02

   Plasmid

   #204500

   Purpose

   Expression of VHL (54-213) for production of VCB complexes in conjunction with plasmid pIVM_26 (EloB 1-104, EloC 17-112)

   Depositor

   Alessio Ciulli

   Article

   Cardote et al Structure. 2017 Jun 6;25(6):901-911.e3.

   Insert

   amino acids 54-213 VHL (VHL Human)
   

   Tags

   6xHis, TEV, and Thrombin

   Expression

   Bacterial

   Mutation

   0

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFA6a-link-ymNeongreen-URA3

   Plasmid

   #168059

   Purpose

   Replaces Addgene Plasmid 125703. Yeast tagging vector to create a gene fusion with yeast optimized mNeongreen with CaUra3 selection; Contains the linker region at the 5’ end of the FP

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymNeonGreen
   

   Tags

   ymNeonGreen

   Expression

   Yeast

   Available Since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. PKNOX1_pCSdest

    Plasmid

    #53899

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    PKNOX1 (PKNOX1 Human)
    

    Expression

    Mammalian

    Promoter

    SP6 and CMV

    Available Since

    Sept. 8, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

11. FU-tetO-Gateway

    Plasmid

    #43914

    Depositor

    John Gearhart

    Article

    Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector, doxycycli…

    Tags

    6xHis and V5

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet-ON

    Available Since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. EF1a-dCasRx-RBM25

    Plasmid

    #221001

    Purpose

    Transient expression of dCasRx-RBM25. EF1a promoter.

    Depositor

    Mikko Taipale, Benjamin Blencowe

    Article

    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

    Insert

    dCasRx-RBM25
    

    Use

    CRISPR

    Tags

    NLS and NLS-HA

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. TREER-Actin

    Plasmid

    #208330

    Purpose

    TREER genetically-encoded tag introduced before actin; to be used with TinyERRE probe peptide.

    Depositor

    Kimberly Beatty

    Article

    Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

    Insert

    TREER-Actin
    

    Tags

    TREER

    Expression

    Mammalian

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. TOMM20-PuRRRE

    Plasmid

    #208329

    Purpose

    PuRRRE genetically-encoded tag introduced after TOMM20; to be used with PunyEEER probe peptide.

    Depositor

    Kimberly Beatty

    Article

    Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

    Insert

    TOMM20-PuRRRE
    

    Tags

    PuRRRE

    Expression

    Mammalian

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. mCh-Rab5

    Plasmid

    #49201

    Purpose

    expression of Rab5 fused to mCherry

    Depositor

    Gia Voeltz

    Article

    Friedman et al J Cell Biol. 2010 Aug 9;190(3):363-75. d

    Insert

    Rab5 (RAB5B Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pICH-nuc-roGFP2-ORP1

    Plasmid

    #191758

    Purpose

    Ti plasmid pICH86988 harbouring nuc-roGFP2-ORP1 for peroxide detection in the nuclei of plant cells.

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

    Insert

    nuc-roGFP2-ORP1
    

    Use

    Binary agrobacterium ti plasmid

    Expression

    Plant

    Promoter

    CaMV 35S

    Available Since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLIX403-hNOS2

    Plasmid

    #110800

    Purpose

    tet-inducible NOS2 expression

    Depositor

    Edward Morgan

    Article

    Lee et al Free Radic Biol Med. 2017 Jul;108:478-48

    Insert

    NOS2 (NOS2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. ampl43

    Plasmid

    #161830

    Purpose

    pRS416-dCas9-Mxi1+TetR+pRPR1(TetO)-NotI-gRNA plasmid marked with His, expressing dCas9-Mxi1 under Tef1 promoter, and a tet-inducible promoter for gRNA expression.

    Depositor

    Saeed Tavazoie

    Article

    Momen-Roknabadi et al Commun Biol. 2020 Nov 27;3(1):723. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. sgOpti

    Plasmid

    #85681

    Purpose

    Lentiviral vector to express sgRNAs. The cloning site is BsmBI.

    Depositor

    Eric Lander, David Sabatini

    Article

    Fulco et al Science. 2016 Sep 29. pii: aag2445.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. PNCA-GFP-dYY1

    Plasmid

    #67791

    Purpose

    Use together with pCMV-intron and VSV-G to package MMLV reporter virus

    Depositor

    Stephen Goff

    Article

    Schlesinger et al Cell Rep. 2013 Jul 11;4(1):50-8. doi: 10

    Insert

    MMLV-LTR-GFP
    

    Mutation

    Deletion of LTR U3 YY1 binding domain

    Available Since

    Sept. 1, 2015

    Availability

    Academic Institutions and Nonprofits only