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pTet-O-ATOH1-T2A-PuroR

Plasmid

#162342

Purpose

Lentiviral expression of ATOH1 under the control of the TetON promoter

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

ATOH1 (ATOH1 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Promoter

Tet-ON

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-p38beta

Plasmid

#86833

Purpose

mammalian expression of GFP-p38beta

Depositor

Rony Seger

Article

Zehorai et al Mol Cell Biol. 2014 Jan;34(2):259-70. do

Insert

p38beta (MAPK11 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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mP58.FL1-pCDNA3

Plasmid

#21883

Depositor

David Ron

Article

Oyadomari et al Cell. 2006 Aug 25. 126(4):727-39.

Insert

P58 (Dnajc3 Mouse)

Expression

Mammalian

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pMSCVneo-DEST

Plasmid

#119746

Purpose

Allows Gateway cloning of gene of interest into retroviral pMSCV vector (with Neomycin/G418 resistance)

Depositor

Andrew Jackson, Martin Reijns

Article

Harley et al Nat Genet. 2016 Jan;48(1):36-43. doi: 10

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-iGluSnFR4s-NGR-WPRE

Plasmid

#234451

Purpose

AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

Depositor

GENIE Project

Article

Aggarwal et al Nat Methods. 2026 Feb;23(2):417-425. doi

Insert

iGluSnFR4s-NGR

Use

AAV

Tags

IgK chain and Myc epi tag-NGR GPI anchor

Expression

Mammalian

Promoter

CAG

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-13Myc-His3MX6

Plasmid

#41602

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

His3

Use

Yeast genomic targeting

Tags

13Myc, A. gossypii translation elongation factor …

Available Since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pCAG-EGFP/RFP-miR-Scrint

Plasmid

#19821

Depositor

Zuoshang Xu

Article

Qiu et al BMC Biotechnol. 2008 Oct 7;8:77. doi: 10

Insert

Scrambled miRNA

Use

Cre/Lox and RNAi

Expression

Mammalian

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-ASAP2s

Plasmid

#101276

Purpose

Genetically encoded voltage indicator (GEVI) ASAP2s in AAV vector for neuron-specific expression using the promoter hSyn1

Depositor

Francois St-Pierre

Article

Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

Insert

ASAP2s

Use

AAV

Expression

Mammalian

Promoter

hSyn (human synapsin)

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-GST-eIF4E K119A

Plasmid

#112818

Purpose

Expresses N-terminally GST-tagged mouse Eif4e K119A in bacterial cells

Depositor

Daniel Schoenberg

Article

Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

Eif4e (Eif4e Mouse)

Tags

GST

Expression

Bacterial

Mutation

K119 mutated to A, increasing affinity for cap st…

Promoter

T7

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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BRCA1 A10.2 gRNA

Plasmid

#90549

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRCA1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

BRCA1 (Guide Designation A10.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3 MDM2 1-400

Plasmid

#45754

Depositor

Robert Lefkowitz

Article

Shenoy et al Science. 2001 Nov 9;294(5545):1307-13. E

Insert

MDM2 1-400 (MDM2 Human)

Expression

Mammalian

Mutation

aa 1-400, lacks catalytic RING domain

Promoter

CMV

Available Since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only
FLAG-SENP6m

Plasmid

#18716

Depositor

Edward Yeh

Article

Dou et al Mol Cell. 2010 Aug 13. 39(3):333-45.

Insert

FLAG-SENP6m (SENP6 Human)

Tags

FLAG

Expression

Mammalian

Mutation

C1030A

Available Since

June 20, 2008

Availability

Academic Institutions and Nonprofits only
HLA_A0101

Plasmid

#165009

Purpose

Expression of HLA-A*01:01 cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

HLA-A*01:01 (HLA-A Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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PIA900

Plasmid

#104401

Purpose

Polycistronic vector for expression of E. coli core RNAP

Depositor

Irina Artsimovitch

Article

Svetlov et al Methods Mol Biol. 2015;1276:13-29. doi:

Insert

rpoA, B, C, and Z

Use

E. coli expression vector

Tags

encodes a C-terminally His-tagged rpoC which can …

Expression

Bacterial

Promoter

T7

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCFD7

Plasmid

#73916

Purpose

gRNA vector for AsCpf1

Depositor

Simon Bullock

Article

Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

Insert

tRNA:hAsCpf1-gRNA:tRNA

Use

CRISPR

Expression

Insect

Promoter

dU6:3

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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HNF1B_pLX307

Plasmid

#98342

Purpose

Lentiviral expression of HNF1B

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

HNF1B (HNF1B Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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TGFbetaR1 (T204D)-pcw107-V5

Plasmid

#64629

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

TGFBR1 (transcript variant 1) (TGFBR1 Human)

Use

Lentiviral

Tags

V5

Mutation

T204D

Promoter

PGK

Available Since

June 1, 2015

Availability

Academic Institutions and Nonprofits only
pTYF-1xGfaABC1D-EGFP

Plasmid

#19974

Depositor

Sergey Kasparov

Article

Liu et al BMC Biotechnol. 2008 May 16;8:49. doi: 1

Insert

GfaABC1D-EGFP

Expression

Mammalian

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pDV06_feedback_entry_TagBFP-2A-MCS-2A-mNeonGreen-2A-MCP-ADAR2dd(E488Q)-NES

Plasmid

#200402

Purpose

Entry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon target detection

Depositor

James Collins

Article

Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

Insert

Closed-loop DART VADAR sensor entry cassette

Use

Synthetic Biology

Expression

Mammalian

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pTUM4

Plasmid

#230905

Purpose

Plasmid encoding four periplasmic folding helper proteins to boost the secretion of functional heterologous proteins in E. coli

Depositor

Arne Skerra

Article

Schlapschy et al Protein Eng Des Sel. 2006 Aug;19(8):385-

Inserts

FkpA peptidyl-prolyl cis-trans isomerase FkpA (fkpA E. coli)
SurA (surA E. coli)
DsbA (dsbA E. coli)
DsbC (dsbC E. coli)

Expression

Bacterial

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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