We narrowed to 30,447 results for: Ide

1. pU6-DHFR

   Plasmid

   #80329

   Purpose

   Allows for expression of an sgRNA in Toxoplasma gondii and confers resistance to pyrimethamine

   Depositor

   Sebastian Lourido

   Article

   Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

   Insert

   DHFR-TSc3
   

   Use

   CRISPR

   Promoter

   DHFR-TS 5' UTR

   Available Since

   Sept. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. plenti-X1-Neo-RPN1-HA

   Plasmid

   #139868

   Purpose

   Lentiviral expression of HA-tagged (C terminal) human RPN1

   Depositor

   Jacob Corn

   Article

   Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

   Insert

   RPN1 (Ribophorin1) (RPN1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Available Since

   July 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3 Flag TSC2 (T1462A)

   Plasmid

   #14130

   Depositor

   Brendan Manning

   Article

   Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

   Insert

   TSC2 T1462A (TSC2 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   T1462A

   Available Since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pLenti-X1-Neo-TEX264-HA

   Plasmid

   #139866

   Purpose

   Lentiviral expression of HA tagged (C terminal) human TEX264

   Depositor

   Jacob Corn

   Article

   Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

   Insert

   TEX264 (TEX264 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Available Since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. p-mCherry-C1-GtACR1

   Plasmid

   #85464

   Purpose

   Natural anion conducting Channelrhodopsin with cyan absorption. Closing kinetics 140ms. High conductivity. Codon optimized for mammalian expression (human/mouse)

   Depositor

   Peter Hegemann

   Article

   Wietek et al J Biol Chem. 2016 Feb 19;291(8):4121-7.

   Insert

   Synthetic construct ACR1 gene
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV (+enhancer)

   Available Since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. p-mCherry-C1-GtACR2

   Plasmid

   #85463

   Purpose

   Natural anion conducting Channelrhodopsin with blue absorption. Closing kinetics 60ms. High conductivity. Codon optimized for mammalian expression (human/mouse)

   Depositor

   Peter Hegemann

   Article

   Wietek et al J Biol Chem. 2016 Feb 19;291(8):4121-7.

   Insert

   Synthetic construct ACR2 gene
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV (+enhancer)

   Available Since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pU6-SAG1-DHFR

   Plasmid

   #80322

   Purpose

   Encodes a CRISPR sgRNA that allows for mutation of the surface antigen SAG1 in Toxoplasma gondii and confers resistance to pyrimethamine

   Depositor

   Sebastian Lourido

   Article

   Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

   Insert

   SAG1 sgRNA
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   Sept. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-phsyn-FLEX-KASH-EGFP-U6-shRNA

   Plasmid

   #129708

   Purpose

   Express cre-dependent KASH-EGFP and scramble shRNA

   Depositor

   Yi Zhang

   Article

   Tuesta et al Nat Commun. 2019 Jun 7;10(1):2508. doi:

   Insert

   KASH-EGFP
   

   Use

   AAV

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-phsyn-FLEX-KASH-EGFP-U6-ShGmeb1

   Plasmid

   #129706

   Purpose

   Express cre-dependent KASH-EGFP and Gmeb1 shRNA

   Depositor

   Yi Zhang

   Article

   Tuesta et al Nat Commun. 2019 Jun 7;10(1):2508. doi:

   Insert

   KASH-EGFP
   

   Use

   AAV

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-GGG-ELP(120nm)-Cys

    Plasmid

    #91572

    Purpose

    Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine

    Depositor

    Hermann Gaub

    Article

    Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

    Insert

    GGG-ELP(120nm)-Cys
    

    Tags

    Cysteine and Sortase-Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAP03-ermE*p

    Plasmid

    #120234

    Purpose

    Carries promoter cassette containing ermE*p promoter and ampicillin resistance gene that can be amplified with homology sequence for refactoring.

    Depositor

    Bradley Moore

    Article

    Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

    Insert

    promoter cassette containing ermE*p promoter and ampicillin resistance gene
    

    Use

    Synthetic Biology

    Mutation

    bla (amp resistance gene)

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBGSA FPR1-EGFP

    Plasmid

    #62604

    Purpose

    Constitutive expression of FPR1-EGFP in mammalian cells

    Depositor

    Heini Miettinen

    Article

    Gripentrog et al J Immunol. 2003 Sep 15;171(6):3187-93.

    Insert

    FPR1 (FPR1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    SRalpha

    Available Since

    June 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX6P1-hPREP

    Plasmid

    #179387

    Purpose

    Express human prolyl oligopeptidase (tagged) in bacterial cells

    Depositor

    Christian Gruber

    Article

    Retzl et al J Nat Prod. 2020 Nov 25;83(11):3305-3314

    Insert

    human prolyl oligopeptidase (PREP Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    TAC

    Available Since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLicC-MBP-APETx2

    Plasmid

    #72668

    Purpose

    Periplasmic expression of APETx2 in E.coli

    Depositor

    Glenn King

    Article

    Anangi et al Mar Drugs. 2012 Jul;10(7):1605-18. doi:

    Insert

    APETx2
    

    Tags

    His tag, MBP, MalE signal sequence for periplasmi…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. p8103 LentiCRISPR v2 sgNT-2

    Plasmid

    #163313

    Purpose

    Expresses Cas9 and a non-targeting control guide RNA

    Depositor

    Elizabeth White

    Article

    Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

    Insert

    sgNT-2
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pWaldo-GFPe_PepTSo

    Plasmid

    #58334

    Purpose

    Expresses the POT family transporter from Shewanella oniedensis in E. coli as a C-terminally tagged GFP his tagged protein

    Depositor

    So Iwata, Simon Newstead

    Article

    Newstead et al EMBO J. 2011 Jan 19;30(2):417-26. doi: 1

    Insert

    PepTso
    

    Tags

    GFP-His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pET28a-Cys-ELP(120nm)-LPETGG

    Plasmid

    #90472

    Purpose

    Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGG

    Depositor

    Hermann Gaub

    Article

    Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

    Insert

    Cys-ELP(120nm)-LPETGG
    

    Tags

    Cysteine and Sortase-Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHS0663 pET45b(+) KraIscB-1 TwinStrep

    Plasmid

    #176536

    Purpose

    Bacterial expression of KraIscB-1-TwinStrep

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Insert

    KraIscB-1
    

    Tags

    TwinStrep

    Expression

    Bacterial

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. p-mCherry-C1-PsACR1

    Plasmid

    #85465

    Purpose

    Natural anion conducting Channelrhodopsin with red shifted absorption. Closing kinetics 80ms. Codon optimized for mammalian expression (human/mouse)

    Depositor

    Peter Hegemann

    Article

    Wietek et al J Biol Chem. 2016 Feb 19;291(8):4121-7.

    Insert

    Synthetic construct PsChR1 gene
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV (+enhancer)

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLL3.7-K122-TEAD-p-H2B-mCherry

    Plasmid

    #139648

    Purpose

    Reporter assay

    Depositor

    Yutaka Hata

    Article

    Kawano et al J Biochem. 2015 May 14. pii: mvv051.

    Insert

    IRES-GFP
    

    Use

    Lentiviral

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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