We narrowed to 76,952 results for: rest

1. pTagBFP-Akt1

   Plasmid

   #86594

   Purpose

   Expresses TagBFP-tagged human Akt1(WT)

   Depositor

   Thomas Leonard, Ivan Yudushkin

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt1 (AKT1 Human)
   

   Expression

   Mammalian

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET17b-yebST

   Plasmid

   #175804

   Purpose

   Complementation of yebST knockout in E. coli K-12

   Depositor

   Gira Bhabha, Damian Ekiert, Ian Henderson

   Article

   Isom et al Sci Rep. 2017 Aug 17;7(1):8608. doi: 10.

   Insert

   yebST
   

   Tags

   None

   Expression

   Bacterial

   Mutation

   silent mutations to introduce restriction sites

   Promoter

   T7

   Available Since

   April 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. psiCHECK2-miR-34 WT

   Plasmid

   #78258

   Purpose

   psiCHECK2 dual luciferase reporter harboring a fully complementary miR-34 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene

   Depositor

   Joanne Weidhaas

   Article

   Salzman et al Nat Commun. 2016 Mar 21;7:10954. doi: 10

   Insert

   fully complimentary miR-34 target site
   

   Use

   Luciferase; Microrna activity

   Expression

   Mammalian

   Available Since

   June 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. EC71100

   Plasmid

   #154068

   Purpose

   Level 0 Golden Gate vector, pHvHSP17

   Depositor

   Cristobal Uauy

   Article

   Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

   Insert

   pHvHSP17
   

   Use

   Standard cloning vector used by geneart for synth…

   Mutation

   All BsaI, Esp3I and BpiI restriction sites were r…

   Available Since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBabe_puro_DEST_Flag_TNIK

   Plasmid

   #45276

   Depositor

   William Hahn

   Article

   Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

   Insert

   TNIK (TNIK Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   May 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLAC

   Plasmid

   #45072

   Purpose

   lacritin-intein fusion plasmid coding for intact mature lacritin without signal peptide

   Depositor

   Gordon Laurie

   Article

   Wang et al J Cell Biol. 2006 Aug 28;174(5):689-700.

   Insert

   Lacritin (LACRT Human)
   

   Use

   Protein expression vector

   Tags

   Intein

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTRY-YFP

   Plasmid

   #15302

   Depositor

   William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   YFP
   

   Use

   Gateway entry

   Available Since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pET-21a_2xNLS_LbCpf1_protein_expression

   Plasmid

   #114366

   Purpose

   Protein expression plasmid for 2xNLS LbCpf1 in pET-21a backbone

   Depositor

   Scot Wolfe

   Article

   Xu et al Blood. 2019 Jan 31. pii: blood-2019-01-8

   Insert

   2xNLS_LbCpf1
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL3-B-pGAS5

   Plasmid

   #46371

   Depositor

   Rolf Stahel, Emanuela Felley-Bosco

   Article

   Renganathan et al Mol Cancer. 2014 May 23;13:119. doi: 10.

   Insert

   GAS5 (GAS5 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Mutation

   promoter

   Available Since

   May 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. Tom20-mCherry-GAI(1-92)

    Plasmid

    #37316

    Depositor

    Takanari Inoue

    Article

    Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

    Insert

    GAI (GAI Mustard Weed)
    

    Tags

    Tom20 and mCherry

    Expression

    Mammalian

    Mutation

    contains aa 1-92

    Promoter

    CMV

    Available Since

    June 15, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pGLucIPZ

    Plasmid

    #228703

    Purpose

    Constitutive expression of Gaussia luciferase, puromycin N-acetyl-transferase and shRNAmir of interest

    Depositor

    Thorsten Stiewe

    Article

    Charles et al Nat Commun. 2014 Jun 3;5:3981. doi: 10.1

    Inserts

    Gaussia luciferase
    non-silencing shRNA
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 2, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. EC71022

    Plasmid

    #159608

    Purpose

    Level 0.5 Golden Gate Vector, loxP-mCHERRY-HDEL-t35s-loxP

    Depositor

    Cristobal Uauy

    Article

    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

    Insert

    loxP-mCherry-HDEL-t35S-loxP
    

    Use

    Cre/Lox and Synthetic Biology

    Mutation

    All BsaI, Esp3I and BPiI restriction sites were r…

    Available Since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDragon-empty

    Plasmid

    #155018

    Purpose

    Empty Tigre-Dragon vector. Genes of interest can be cloned using Mlu-I and SnaB-I. For Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA.

    Depositor

    Alberto Rosello-Diez

    Article

    Ahmadzadeh et al Development. 2020 May 28;147(10). pii: d

    Type

    Empty backbone

    Use

    Mouse Targeting

    Available Since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6m-WPRE-pA

    Plasmid

    #51473

    Purpose

    Bicistronic vector expressing mRuby2 and GCaMP6m from a single open reading frame.

    Depositor

    Mark Huebener, Tobias Rose, Tobias Bonhoeffer

    Article

    Rose et al Science. 2016 Jun 10;352(6291):1319-22.

    Insert

    mRuby2-P2A-GCaMP6m
    

    Use

    AAV

    Promoter

    hSyn1

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. 3xFLAG-dCas9/pMSCVpuro

    Plasmid

    #134983

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR and Retroviral

    Tags

    3xFLAG tag and NLS

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    LTR

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. C1-MPAct(delta1-6)-mRuby3

    Plasmid

    #155223

    Purpose

    F-actin bidning mutant of MPAct

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    Mutated version of F-tractin (aa 15-52 of rat ITPKA) fused to mRuby3 C-term tagged with the CaaX sequence derived from Kras4b
    

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Deletion of first 6 AA from F-tractin (9-14 from …

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. A3Bi-Cas9n-UGI

    Plasmid

    #119135

    Purpose

    Base editor made from full-length APOBEC3B with an L1 intron to prevent self-restriction

    Depositor

    Reuben Harris

    Article

    Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

    Insert

    APOBEC3B L1 intron-Cas9 nickase-UGI
    

    Use

    CRISPR

    Mutation

    L1 intron insertion

    Promoter

    CMV

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. C1-FT-mCitrine-EAAARx8-CaaX

    Plasmid

    #155224

    Purpose

    MPAct with increased search radius

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    F-tractin (aa9-52 of rat ITPKA) fused to mCitrine C-term tagged with codon optimized (EAAAR)x8 and CaaX seq derived from Kras4b
    

    Tags

    mCitrine

    Expression

    Mammalian

    Available Since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSUPERIORpuro-shGAS5

    Plasmid

    #46370

    Depositor

    Rolf Stahel, Emanuela Felley-Bosco

    Article

    Renganathan et al Mol Cancer. 2014 May 23;13:119. doi: 10.

    Insert

    GAS5 (GAS5 Human)
    

    Use

    RNAi

    Promoter

    H1

    Available Since

    Aug. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pCRISPReporter

    Plasmid

    #65007

    Purpose

    E. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with reporter cloning site

    Depositor

    Mattheos Koffas

    Article

    Cress et al ACS Synth Biol. 2015 Mar 30.

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    none

    Available Since

    May 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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