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pAc5.1B-EGFP-DmDCP1_1-127_A

Plasmid

#145901

Purpose

Insect Expression of DmDCP1_1-127

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmDCP1_1-127 (Dcp1 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmDCP1_128-372_A

Plasmid

#145902

Purpose

Insect Expression of DmDCP1_128-372

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmDCP1_128-372 (Dcp1 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmDCP1_1-127_A

Plasmid

#145903

Purpose

Insect Expression of DmDCP1_1-127

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmDCP1_1-127 (Dcp1 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmDCP1_128-372_A

Plasmid

#145904

Purpose

Insect Expression of DmDCP1_128-372

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmDCP1_128-372 (Dcp1 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pnYC-NvHN-DmXRN1_1323-1355_N

Plasmid

#147052

Purpose

Bacterial Expression of DmXRN1_1323-1355

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmXRN1_1323-1355 (pcm Fly)

Expression

Bacterial

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pYZ_NAND4_L17_S24_S11_S19_S13

Plasmid

#132732

Purpose

T7 RNAP-driven expression of a 4-input NAND gate RNA with a GFP-ASV reporter that binds to 3WJ repressor trigger indices 24, 11, 19, and 13

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

3WJ_NAND4_L17_S24_S11_S19_S13

Expression

Bacterial

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pYZ_NAND3_L11_S11_S13_S19

Plasmid

#132731

Purpose

T7 RNAP-driven expression of a 3-input NAND gate RNA with a GFP-ASV reporter that binds to 3WJ repressor trigger indices 11, 13, and 19

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

3WJ_NAND3_L11_S11_S13_S19

Expression

Bacterial

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pFastBac-LacI-LAP-HEC1

Plasmid

#114033

Purpose

Production of baculovirus with LacI-LAP-HEC1

Depositor

Geert Kops

Article

Hiruma et al Science. 2015 Jun 12;348(6240):1264-7. d

Insert

LacI-LAP-HEC1 (NDC80 Human)

Expression

Insect

Mutation

none

Promoter

CMV

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pET22b-ecDHFR-dCys-T46C

Plasmid

#110543

Purpose

Bacterial expression of E.coli DHFR C85A/C152S/T46C mutant

Depositor

Stephen Benkovic

Article

Liu et al J Am Chem Soc. 2014 Jul 23;136(29):10349

Insert

ecDHFR

Expression

Bacterial

Mutation

T46 mutated to C46, C85 mutated to A85, C152 muta…

Promoter

T7

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pET22b-ecDHFR-dCys-L54C

Plasmid

#110542

Purpose

Bacterial expression of E.coli DHFR C85A/C152S/L54C mutant

Depositor

Stephen Benkovic

Article

Liu et al J Am Chem Soc. 2014 Jul 23;136(29):10349

Insert

ecDHFR

Expression

Bacterial

Mutation

L54 mutated to C54, C85 mutated to A85, C152 muta…

Promoter

T7

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP eIF2beta C-term (aa 89-331)

Plasmid

#45901

Depositor

Louise Larose

Article

Kebache et al Proc Natl Acad Sci U S A. 2002 Apr 16;99

Insert

eIF2S2 aa89-331 (Eif2s2 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

aa 89-331

Promoter

CMV

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
pCDNA3 T7 BimL(S58D,DE)

Plasmid

#25057

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (S58D,DE) (BCL2L11 Human)

Tags

T7

Expression

Mammalian

Mutation

Changed serine 58 to aspartic acid changed leuci…

Available Since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 T7 BimL(S44A,DE)

Plasmid

#25054

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (S44A,DE) (BCL2L11 Human)

Tags

T7

Expression

Mammalian

Mutation

Changed serine 44 to alanine changed leucine 92 …

Available Since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 T7 BimL(T56A,DE)

Plasmid

#25055

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (T56A,DE) (BCL2L11 Human)

Tags

T7

Expression

Mammalian

Mutation

Changed threonine 56 to alanine changed leucine …

Available Since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 T7 BimL(S58A,DE)

Plasmid

#25056

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (S58A,DE) (BCL2L11 Human)

Tags

T7

Expression

Mammalian

Mutation

Changed serine 58 to alanine changed leucine 92 …

Available Since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag BimL(S44D,DE)

Plasmid

#25058

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (S44D,DE) (BCL2L11 Human)

Tags

Flag

Expression

Mammalian

Mutation

Changed serine 44 to aspartic acid changed leuci…

Available Since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag BimL(S58D,DE)

Plasmid

#25061

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (S58D,DE) (BCL2L11 Human)

Tags

Flag

Expression

Mammalian

Mutation

Changed serine 58 to aspartic acid changed leuci…

Available Since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
pPICZ:hTRAAK(W262S)

Plasmid

#130670

Purpose

P. pastoris expression vector. It will generate the human TRAAK channel (1-300) fused to a C-terminal GFP

Depositor

Dan Minor

Article

Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

Insert

KCNK4 (KCNK4 Human)

Tags

SNS- 3C_cleavage_site-TAAA-GFP

Expression

Yeast

Mutation

N104Q, N108Q, W262S

Availability

Academic Institutions and Nonprofits only

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pIRES:hTRAAK(W262S)

Plasmid

#133078

Purpose

Mammalian expression vector. It will generate the human TRAAK channel full lenght W262S mutant

Depositor

Dan Minor

Article

Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

Insert

Potassium channel subfamily K member 4 (KCNK4, TRAAK) W262S (KCNK4 Human)

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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pIRES:hTRAAK(G124I)

Plasmid

#133079

Purpose

Mammalian expression vector. It will generate the human TRAAK channel full lenght G124I mutant

Depositor

Dan Minor

Article

Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

Insert

Potassium channel subfamily K member 4 (KCNK4, TRAAK) G124I (KCNK4 Human)

Expression

Mammalian

Mutation

G124I

Availability

Academic Institutions and Nonprofits only

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HM3C NaVSp1 pET24b

Plasmid

#128088

Purpose

Bacterial expression vector for NaVSp1 bacterial sodium channel. It expresses for HIStag-MBP-3C-channel protein

Depositor

Dan Minor

Article

Shaya et al Proc Natl Acad Sci U S A. 2011 Jul 26;10

Insert

NaVSp1p

Tags

3C Cleavage, His Tag, and MBP

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

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pLentiFUCCI(CA)5

Plasmid

#223176

Purpose

Lentiviral fluorescent ubiquitination-based cell cycle indicator (FUCCI)

Depositor

Neville Sanjana

Article

Liang et al Cell. 2024 Dec 26;187(26):7637-7654.e29.

Insert

FUCCI(CA)5

Use

Lentiviral

Tags

AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)

Expression

Mammalian

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-TadA8e-PcrA M6

Plasmid

#221229

Purpose

Express HACE Editor as a fusion of TadA-8e adenosine deaminase and an optimized PcrA M6 helicase.

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

TadA8e-PcrA M6

Expression

Mammalian

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pLNCX2 ER:ras

Plasmid

#67844

Purpose

4-hydroxytamoxifen inducible ER:ras fusion protein

Depositor

Masashi Narita

Article

Young et al Genes Dev. 2009 Apr 1;23(7):798-803. doi

Insert

ER1a H-RasG12V fusion

Expression

Mammalian

Mutation

G525R renders the HBD largely insensitive to 17ß‐…

Promoter

CMV

Available Since

Aug. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pLPCX cyto Grx1-roGFP2

Plasmid

#64975

Purpose

Mammalian expression of cytosolic Grx1-roGFP2 (retroviral vector)

Depositor

Tobias Dick

Article

Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

Insert

Glutaredoxin-1 (GLRX Human)

Use

Retroviral

Tags

roGFP2

Expression

Mammalian

Available Since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-SpCas9(D10A)-T2A-EGFP

Plasmid

#221230

Purpose

Express nCas9 nickase (D10) with GFP reporter

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

SpCas9(D10A)-T2A-EGFP

Expression

Mammalian

Mutation

D10A

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-EFS-hfCas13d-HA-mCherry-pA

Plasmid

#233029

Purpose

To Express HA Tagged hfCas13d-mCherry fusion protein from the mammalian EFS promoter

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

hfCas13d-mCherry

Use

AAV

Tags

mCherry

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-CMV-NFE2L2-Puro

Plasmid

#181919

Purpose

Lentiviral plasmid that directs the expression of NFE2L2/Nrf2 in mammalian cells.

Depositor

Scott Dixon

Article

Forcina et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

Insert

NFE2L2 (NFE2L2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
8522-M01-663

Plasmid

#225660

Purpose

Lentiviral expression of a cancer stem cell reporter that responds to presence of Sox2/Oct4 or their paralogs (green configuration)

Depositor

Dominic Esposito

Article

Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

Insert

dsCopGFP

Use

Lentiviral

Promoter

SORE6-mCMVp

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
(559) pAAV EF-1a KRAB-SadCas9 U6-gRNA

Plasmid

#163024

Purpose

Expression of CRISPRi with U6-gRNA (Bsa1 sites)

Depositor

Tonia Rex

Article

Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

Insert

Mammalian codon-optimized SadCas9

Use

AAV

Tags

KRAB, SV40 polyA, and myc NLS

Expression

Mammalian

Promoter

hEF-1a

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSF4 TetCMV ATF4(5'UTR)-SunTag-Renilla-FKBP-Stop-24xMS2v5-CTE-polyA

Plasmid

#164615

Purpose

Expresses ATF4(5'UTR)-SunTag-Renilla mRNA reporter under tetracycline-inducible promoter. SunTag and Renilla luciferase are in frame with the main start codon of ATF4.

Depositor

Jeffrey Chao

Article

Mateju et al Cell. 2020 Dec 23;183(7):1801-1812.e13.

Insert

Renilla luciferase

Tags

24xMS2v5 (3'UTR) and SunTag

Expression

Bacterial and Mammalian

Promoter

TetCMV

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
8522-M31-663

Plasmid

#225661

Purpose

Lentiviral expression of a cancer stem cell reporter that responds to presence of Sox2/Oct4 or their paralogs (red configuration)

Depositor

Dominic Esposito

Article

Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

Insert

dsCherry

Use

Lentiviral

Promoter

SORE6-mCMVp

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
OMMGFP 1-10

Plasmid

#164119

Purpose

Expresses the non-fluorescent GFP1-10 moiety on the cytosolic face of the OMM

Depositor

Marisa Brini, Tito Calì

Article

Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

Insert

OMMGFP 1-10

Expression

Mammalian

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
8138-E02

Plasmid

#225659

Purpose

Gateway clone for SORE6 reporter (6 repeats of a SOX2/OCT4 response element from the human NANOG promoter)

Depositor

Dominic Esposito

Article

Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

Insert

SORE6

Use

Gateway entry clone

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLPCX mito Grx1-roGFP2

Plasmid

#64977

Purpose

Mammalian expression of mitochondrial Grx1-roGFP2 (retroviral vector)

Depositor

Tobias Dick

Article

Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

Insert

Glutaredoxin-1 (GLRX Human)

Use

Retroviral

Tags

roGFP2

Expression

Mammalian

Mutation

mitochondrial targeting sequence

Available Since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-HA-BACE1

Plasmid

#165032

Purpose

HA-BACE1

Depositor

Juan Bonifacino

Article

Prabhu et al Mol Biol Cell. 2012 Jun;23(12):2339-51.

Insert

BACE1 (BACE1 Human)

Tags

HA (embedded after propeptide cleavage site)

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-U6-DR30(SapI)-CMV-intron-CasRx-pA

Plasmid

#192485

Purpose

To express CasRX and a gRNA

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx

Use

AAV

Mutation

No

Promoter

CMV

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
HiBiT-FZD7

Plasmid

#195845

Purpose

Expresses human FZD7 with an N-terminal HiBiT

Depositor

Gunnar Schulte

Article

Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

Insert

FZD7 (FZD7 Human)

Tags

HiBiT

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR223-BALF4

Plasmid

#195835

Purpose

Gateway Entry Clone of Epstein-Barr virus BALF4

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BALF4 (BALF4 Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCG-TPMV Hd32-IL-13

Plasmid

#163869

Purpose

Expresses TPMV H glycoprotein bearing IL-13 in mammalian cells

Depositor

Jakob Reiser

Article

Argaw et al Mol Ther Methods Clin Dev. 2021 Apr 24;2

Insert

TPMV H glycoprotein/IL-13

Tags

Human IL-13

Expression

Mammalian

Mutation

Amino acids 2 to 33 at N terminus deleted

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-SpCas9(H840A)-T2A-EGFP

Plasmid

#221231

Purpose

Express nCas9 nickase (H840A) with GFP reporter

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

SpCas9(H840A)-T2A-EGFP

Expression

Mammalian

Mutation

H840A

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
psiCHECK2-let-7 MT

Plasmid

#78261

Purpose

psiCHECK2 dual luciferase reporter harboring a mutated let-7 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene

Depositor

Joanne Weidhaas

Article

Salzman et al Nat Commun. 2016 Mar 21;7:10954. doi: 10

Insert

mutated let-7 target site

Use

Luciferase; Microrna activity

Expression

Mammalian

Mutation

nucleotides 2-5 and 10-11 changed from CTCC to GA…

Available Since

June 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
HiBiT-FZD3

Plasmid

#195841

Purpose

Expresses human FZD3 with an N-terminal HiBiT; contains I292V and V495I

Depositor

Gunnar Schulte

Article

Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

Insert

FZD3 (FZD3 Human)

Tags

HiBiT

Expression

Mammalian

Mutation

I292V and V495I

Promoter

CMV

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE-60 Grx1-roGFP2-His

Plasmid

#64799

Purpose

Bacterial expression of His-tagged Grx1-roGFP2

Depositor

Tobias Dick

Article

Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

Insert

Glutaredoxin-1 (GLRX Human)

Tags

His and roGFP2

Expression

Bacterial

Available Since

Aug. 19, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
hSOD1-FLAG

Plasmid

#182922

Purpose

Mammalian expression plasmid for human WT SOD1

Depositor

Philip Eaton

Article

Switzer et al Proc Natl Acad Sci U S A. 2023 Jan 17;12

Insert

[Cu-Zn] Superoxide dismutase 1 (SOD1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-SFFV-PYL1-sfGFP-Emerin

Plasmid

#121532

Purpose

expression of PYL-SfGFP-Emerin, lentivirus vector

Depositor

Stanley Qi

Article

Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

Insert

Emerin (EMD Human)

Use

Lentiviral

Tags

PYL1-SFGFP

Expression

Mammalian

Promoter

SFFV

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR223-BRLF1(Rta)

Plasmid

#195824

Purpose

Gateway Entry Clone of Epstein-Barr virus BRLF1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BRLF1 (BRLF1 Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac HT JS-Rab10wt

Plasmid

#135557

Purpose

Express mouse Rab10wt in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Rab10wt.

Depositor

Jingshi Shen

Article

Gulbranson et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

Insert

Rab10 (Rab10 Mouse)

Tags

6xHis tag, TEV site

Expression

Insect

Promoter

polyhedrin promoter

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pQ79

Plasmid

#175064

Purpose

expresses mutant of the huntingtin exon1 in mammalian cells

Depositor

Arnold Boersma

Article

Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

Insert

polyQ79 (HTT Human)

Expression

Mammalian

Mutation

mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…

Promoter

CMV Promoter

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR223-BLLF1(gp350)

Plasmid

#195837

Purpose

Gateway Entry Clone of Epstein-Barr virus BLLF1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BLLF1 (BLLF1 Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 7,325 results for: 11