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pLKO.1 MP1 shRNA

Plasmid

#26632

Depositor

David Sabatini

Article

Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

Insert

MP1 shRNA (LAMTOR3 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Nov. 17, 2010

Availability

Academic Institutions and Nonprofits only
pCI-neo-Myc-hAtg14

Plasmid

#24293

Depositor

Noboru Mizushima

Article

Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

Insert

autophagy related genes 14 (ATG14 Human)

Tags

3xMyc

Expression

Mammalian

Available Since

Sept. 15, 2010

Availability

Academic Institutions and Nonprofits only
pYPQ210

Plasmid

#109329

Purpose

Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and bar gene for plant selection

Depositor

Yiping Qi

Article

Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

Type

Empty backbone

Use

Gateway compatible attr1-attr2 destination vector

Expression

Plant

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCSCherryN5icd

Plasmid

#22456

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

Notch3 (notch3 Zebrafish)

Use

Expression vector

Tags

mCherry

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pPro24(s)-gfp

Plasmid

#17813

Depositor

Jay Keasling

Article

Lee et al Biotechnol Bioeng. 2006 Apr 5. 93(5):912

Insert

GFPuv

Use

Synthetic Biology

Expression

Bacterial

Mutation

D234N compared to AF007834

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
pBS mPax1 (CT#1007)

Plasmid

#13898

Depositor

Cliff Tabin

Article

Brent et al Cell. 2003 Apr 18. 113(2):235-48.

Insert

Pax1 (Pax1 Mouse)

Expression

Bacterial

Available Since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only

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pKLV-EF1a-mCherryBCL11B-W

Plasmid

#208758

Purpose

Lentiviral vector expressing mCherry fused with BCL11B cDNA

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

BCL11B (BCL11B Human)

Use

Lentiviral

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-BSD-tet-mEGFP-MIB1

Plasmid

#140241

Purpose

Lentiviral vector expressing mEGFP-tagged MIB1 upon tetracycline treatment

Depositor

Urs Greber

Article

Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

Insert

Tet-inducible mEGFP-MIB1 (MIB1 Human)

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

TRE

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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mTurquoise2-Rab7(WT)

Plasmid

#112959

Purpose

In vivo visualization of Rab7 (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Rab7

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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HA-hAtg13

Plasmid

#31967

Depositor

Do-Hyung Kim

Article

Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

Insert

Atg13 (ATG13 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV, SP6

Available Since

Sept. 1, 2011

Availability

Academic Institutions and Nonprofits only
pGP-AAV-CAG-FLEX-jGCaMP7c variant 1513-WPRE

Plasmid

#105323

Purpose

AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre-dependent expression

Depositor

Douglas Kim, GENIE Project

Article

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Insert

jGCaMP7c variant 1513

Use

AAV and Cre/Lox

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 23, 2018

Availability

Academic Institutions and Nonprofits only

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G protein alpha-q-YFP

Plasmid

#55782

Purpose

This G protein alpha-q construct contains internal insertions of YFP and the EE epitope.

Depositor

Catherine Berlot

Article

Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

Insert

alpha-q EE YFP (Gnaq Mouse)

Tags

The EE epitope was introduced into the alpha-q se…

Expression

Mammalian

Mutation

BamHI and SacI sites in alpha-q were removed with…

Promoter

CMV

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-evoCjCas9-ABE8e-BlastR

Plasmid

#194060

Purpose

Mammalian expression of evoCjCas9 ABE8e

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-ABE8e

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-evoCjCas9-PEmax_dRnH-BlastR

Plasmid

#194063

Purpose

Mammalian expression of evoCjCas9 PEmax_deltaRnH

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-PEmax_dRnH

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ262B-ABE8e

Plasmid

#161524

Purpose

Gateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 ABE8e mediated A-G base editing

Depositor

Yiping Qi

Article

Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

Insert

TadA8e-zSpRYCas9(D10A)

Use

CRISPR; Gateway compatible tada8e-zsprycas9(d10a)…

Expression

Plant

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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eGFP L202 gRNA

Plasmid

#119132

Purpose

gRNA that guides Cas9 and APOBEC complexes to L202 in eGFP.

Depositor

Reuben Harris

Article

Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

Insert

eGFP L202 gRNA

Use

CRISPR

Mutation

None

Promoter

U6

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pBEVY-GA

Plasmid

#51227

Purpose

bi-directional expression vector for yeast, galactose inducible, ADE2 selection

Depositor

Charles Miller

Article

Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

Type

Empty backbone

Expression

Yeast

Promoter

GAL1,10

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pAR015 Lenti pEF-dEnAsCas12a-NLS-NFZ-3XHA-T2A-BSD

Plasmid

#195544

Purpose

dCas12a effector fusion for manipulating transcription

Depositor

Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

Lenti pEF-dEnAsCas12a-NLS-NFZ-3XHA-T2A-BSD

Use

CRISPR and Lentiviral

Tags

3XHA

Expression

Mammalian

Promoter

pEF1a

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pBFC0996

Plasmid

#186695

Purpose

VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, 2xLguI cargo stuffer, with ET-Seq (AsiSI+SbfI) restriction sites flanking Tn7 Right End

Depositor

Jennifer Doudna

Article

Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

Inserts

tnsA
tnsB
tnsC
tnsD
tniQ
cas8-cas5 fusion
cas7
cas6
crRNA
Tn7 transposon
2xLguI Cargo Stuffer
SbfI+AsiSI dual restriction site for donor plasmid removal during ET-Seq

Expression

Bacterial

Promoter

PLac

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO V5 DNAJA3

Plasmid

#19520

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DnaJ (Hsp40) homolog, subfamily A, member 3 (DNAJA3 Human)

Tags

V5

Expression

Mammalian

Mutation

F193L based on NP_005138.3

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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