We narrowed to 159,134 results for: Gene

1. pcDNA3.1-hRARα

   Plasmid

   #135397

   Purpose

   Human Retinoic Acid Receptor-alpha in pcDNA3.1(+) plasmid expression vector

   Depositor

   Catharine Ross

   Article

   Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

   Insert

   Human retinoic acid receptor-alpha
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSLC-246

   Plasmid

   #73190

   Purpose

   Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (tse2) under the control of rhamnose induceable promoter (PrhaB) for negative selection.

   Depositor

   Swaine Chen

   Article

   Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

   Insert

   PrhaB-tse2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PrhaB

   Available since

   July 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. 4xHRE_minCMV-sfGFP-CMV_dsRed Exp

   Plasmid

   #117401

   Purpose

   sfGFP expressed from hypoxia-inducible minimal CMV promoter and dsRed expressed from consitutive CMV promoter

   Depositor

   Yvonne Chen

   Article

   Ede et al ACS Synth Biol. 2016 May 20;5(5):395-404

   Insert

   SuperFolder GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKO (Kan)

   Plasmid

   #110086

   Purpose

   Suicide vector for generation of gene deletions in mycobacteria. Contains the two genes sacB and galK for negative selection.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Use

   Bacterial knockout generation

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. lentiCRISPR - CTRL sgRNA

   Plasmid

   #70662

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a non-targeting sgRNA element from U6 promoter. Lentiviral backbone

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   non-targeting sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. AZ64_pE.DonorCLYBL.TS

   Plasmid

   #199228

   Purpose

   Donor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locus

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

   Insert

   Expression unit for EGFP reporter
   

   Use

   Gene targeting donor plasmid

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human PGK1 gene promoter

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVL1392 TMEM97

   Plasmid

   #157748

   Purpose

   Baculovirus transfer vectors to create a virus expressing human TMEM97

   Depositor

   Andrew Kruse

   Article

   Alon et al Proc Natl Acad Sci U S A. 2017 Jul 3;114

   Insert

   TMEM97 (TMEM97 Human)
   

   Use

   Tags

   flag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 10, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pcDNA-FLAG-Hu-Trip6

   Plasmid

   #27255

   Depositor

   Thomas Gilmore

   Article

   Zhao et al Gene Expr. 1999 . 8(4):207-17.

   Insert

   Trip6 (TRIP6 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 1, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pEE391

   Plasmid

   #149281

   Purpose

   T-DNA encoding TRV2 with FT augmented gRNA targeting NbPDS3

   Depositor

   Daniel Voytas

   Article

   Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

   Insert

   p35S:TRV2_NbPDS3sgRNA_FT:tNos
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   July 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKB01_mCherry

    Plasmid

    #69104

    Purpose

    Expresses mCherry (codon adapted for Bacillus subtilis); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

    Depositor

    Jan-Willem Veening

    Article

    Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pH6HTC_RBFOX2-Halo

    Plasmid

    #175304

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    RBFOX2 (RBFOX2 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pENTR R4 ATCC LacZ GCTT

    Plasmid

    #173668

    Purpose

    Destination vector for Golden Gate Cloning assemblies, blue-white screening

    Depositor

    Eric Marois

    Article

    Green et al Elife. 2023 Dec 5;12:e93142. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pE478G-EGFP

    Plasmid

    #68848

    Purpose

    mutated OPTN was inserted into pEGFP-N1 backbone

    Depositor

    Beatrice Yue

    Article

    Turturro et al Springerplus. 2014 Feb 19;3:99. doi: 10.

    Insert

    optineurin (OPTN Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    E478G, associated with ALS

    Promoter

    CMV

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMM135

    Plasmid

    #127216

    Purpose

    BeYDV replicon with constitutive Luc expression, WUS2, sgRNA targeting PDS

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    Luc, WUS2, sgRNA
    

    Use

    Tags

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TALEN-mROSA26 KKR

    Plasmid

    #60025

    Purpose

    Expression of a TALEN protein specific for mouse ROSA26 locus.

    Depositor

    Radislav Sedláček

    Article

    Kasparek et al FEBS Lett. 2014 Sep 18. pii: S0014-5793(

    Insert

    TALEN-ROSA26
    

    Use

    TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Nov. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMM146

    Plasmid

    #127218

    Purpose

    BeYDV replicon with constitutive Luc expression, BBM, sgRNA targeting PDS

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    Luc, BBM, sgRNA
    

    Use

    Tags

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBx-Spas-sgRNA-Gm

    Plasmid

    #105232

    Purpose

    Plasmid containing S. pasteurianus sgRNA with no 20bp target (Empty control) Gentamycin marker for P. aeruginosa

    Depositor

    Kristala Prather

    Article

    Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

    Insert

    S. pasteurianus sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUC18-mini-Tn7T-Plac-dCas9 (Plac)

    Plasmid

    #105235

    Purpose

    Tn7 integrating plasmid with S. pasteurianus dCas9 expressed from the Plac promoter for expression in P. aeruginosa

    Depositor

    Kristala Prather

    Article

    Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

    Insert

    S. pasteurianus dCas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    HA

    Expression

    Bacterial

    Mutation

    D10A, H599A

    Promoter

    Available since

    Aug. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAP-BAC

    Plasmid

    #120229

    Purpose

    TAR cloning in yeast and low-copy maintenance of large constructs in E. coli

    Depositor

    Bradley Moore

    Article

    Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-Cas13d-T2A-Citrine

    Plasmid

    #224565

    Purpose

    Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), Cas13d, and Citrine reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    Cas13d-T2A-Citrine
    

    Use

    CRISPR

    Tags

    T2A-Citrine

    Expression

    Mutation

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. U6-(BbsI)sgRNA_CAG-Venus-bpA

    Plasmid

    #86985

    Purpose

    For cloning and expression of sgRNA together with Venus expression

    Depositor

    Ralf Kuehn

    Article

    Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

    Insert

    Venus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Venus GFP variant

    Promoter

    CAG

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCMV-RosaR4 KKR mutations

    Plasmid

    #37199

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nucleic Acids Res. 2012 Apr 1;40(8):3741

    Insert

    FokI (fused to RosaR4 ZFN)
    

    Use

    Zfn expression

    Tags

    HA, ROSA26 eZFNs R4, and SV40 NLS

    Expression

    Mammalian

    Mutation

    I538K, E490K, and H537R

    Promoter

    CMV

    Available since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pUAS-Cre

    Plasmid

    #50797

    Purpose

    Expression of Cre recombinase under the control of the UAS-promoter

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    Cre recombinase
    

    Use

    Multi-species

    Tags

    Expression

    Mutation

    Promoter

    UAS-hsp70

    Available since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCDH-EF1a-HygroR-T2A-TetOn3G

    Plasmid

    #179887

    Purpose

    Expresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)

    Depositor

    Patrick Caswell

    Article

    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

    Insert

    TetOn3G
    

    Use

    Lentiviral

    Tags

    Hygromycin resistance

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCRISPR-S12

    Plasmid

    #84031

    Purpose

    To episomally express codon optimized Cas9 and chimeric guide RNA

    Depositor

    Sen Wu

    Article

    Li et al Protein Cell. 2016 Sep;7(9):689-91. doi:

    Inserts

    hSpCas9
    eGFP
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and CMV (downstream of F2A self-cleaving pept…

    Available since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. cr3Vn

    Plasmid

    #184861

    Purpose

    cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an V. natriegens-specific recombination system

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    Cas9, gam, bet, exo
    

    Use

    Tags

    Cas9 ssrA degradation tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSBbi-TIR1-tTA/Pur

    Plasmid

    #171683

    Purpose

    constitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (puromycin)

    Depositor

    Randy Poon

    Article

    Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.

    Inserts

    tetracycline-controlled transactivator
    Puromycin
    EF-1a promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a promoter and RPBSA promoter

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Myc-CICf

    Plasmid

    #48185

    Purpose

    Expresses Cic

    Depositor

    Huda Zoghbi

    Article

    Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

    Insert

    Capicua (Cic Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pIntegrase_9

    Plasmid

    #60580

    Purpose

    plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Integrase_9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pBAD

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLys-M1

    Plasmid

    #109382

    Purpose

    Arabinose inducible VioB-mCherry expression (stronger RBS). Used to test burden response in E. coli

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Insert

    pARABAD-VioB-mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCXLE-dCas9VP192-T2A-EGFP-shP53

    Plasmid

    #69535

    Purpose

    Episomal plasmid encoding dCas9VP192 and p53 shRNA

    Depositor

    Timo Otonkoski

    Article

    Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

    Insert

    dCas9-dCas9VP192-GFP-shp53
    

    Use

    CRISPR

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Nov. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pH6HTC_ENO1-Halo

    Plasmid

    #175330

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    ENO1 (ENO1 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pKO (Apr)

    Plasmid

    #110088

    Purpose

    Suicide vector for generation of gene deletions in mycobacteria. Contains the two genes sacB and galK for negative selection.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Use

    Bacterial knockout generation

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pHIV-Wnt1HA

    Plasmid

    #18983

    Depositor

    Zena Werb, Bryan Welm

    Article

    Welm et al Cell Stem Cell. 2008 Jan 10. 2(1):90-102

    Insert

    wingless-related MMTV integration site 1 (Wnt1 Mouse)
    

    Use

    Lentiviral

    Tags

    HA-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 24, 2008

    Availability

    Academic Institutions and Nonprofits only

35. pDGE1108

    Plasmid

    #202772

    Purpose

    CRISPR-KO in Arabidopsis thaliana

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

    Inserts

    FAST marker
    pRPS5a:zCas9_ttriple
    ccdB cassette (BsaI-excisable)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAbaMCi-GFP-BsaI_pJMP2748

    Plasmid

    #208888

    Purpose

    Acinetobacter baumannii CRISPRi test plasmid (GFP, BsaI cloning site in gRNA cassette).

    Depositor

    Jason Peters

    Article

    Ward et al mBio. 2023 Dec 21:e0205123. doi: 10.1128

    Insert

    dCas9, sgRNA expression cassette, sfGFP
    

    Use

    Pir dependent origin of replication

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAXY0007

    Plasmid

    #205576

    Purpose

    NpuDnaE(C)-Glucos(232-500)

    Depositor

    Xiaohan Yang

    Article

    Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

    Insert

    NpuDnaE(C)-Glucosyltransferase
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pFD115

    Plasmid

    #124768

    Purpose

    pFD115 carries cas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector

    Depositor

    David Bikard

    Article

    Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)

    Inserts

    Ptet
    cas9
    term PpflB sgRNA (BsaI)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ptet

    Available since

    Aug. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLenti-CMV-puro-2A-TEVp

    Plasmid

    #99610

    Purpose

    Lentivirus expressing puromycin resistance gene and TEV protease

    Depositor

    Marco Tripodi

    Article

    Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

    Inserts

    Puromycin resistance (PAC gene)
    TEV protease
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBSV2_Psyn-sgRNA500

    Plasmid

    #149613

    Purpose

    gRNA expression in B. burgdorferi, parental vector

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Psyn

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pMM131

    Plasmid

    #127214

    Purpose

    BeYDV replicon with constitutive Luc expression, AtSTM, sgRNA targeting PDS

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    Luc, AtSTM, sgRNA
    

    Use

    Tags

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. IL-4 promoter luciferase

    Plasmid

    #12195

    Depositor

    Anjana Rao

    Article

    Macian et al EMBO J. 2000 Sep 1. 19(17):4783-95.

    Insert

    IL-4 promoter (Il4 Mouse)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCaRNA3

    Plasmid

    #86088

    Purpose

    Expression of cDNA of full-length genomic RNA3 of PNRSV (Pch12 isolate) in transfected cells

    Depositor

    Aiming Wang

    Article

    Cui et al Plant Biotechnol J. 2016 Aug 27. doi: 10

    Insert

    RNA3
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pKO (Hyg)

    Plasmid

    #110087

    Purpose

    Suicide vector for generation of gene deletions in mycobacteria. Contains the two genes sacB and galK for negative selection.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Use

    Bacterial knockout generation

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pUCHR-inLuc

    Plasmid

    #58955

    Purpose

    HIV-1 transfer vector, encodes firefly luciferase gene interrupted by a gamma-globin intron. The reporter gene is silent in the transfected cells and expressed in the infected cells.

    Depositor

    Dmitriy Mazurov, David Derse

    Article

    Mazurov et al PLoS Pathog. 2010 Feb 26;6(2):e1000788.

    Insert

    inLuc
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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46. pCMV-RosaL6 ELD mutations

    Plasmid

    #37198

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nucleic Acids Res. 2012 Apr 1;40(8):3741

    Insert

    FokI (fused to Rosa L6 ZFN)
    

    Use

    Zfn expression

    Tags

    HA, ROSA26 eZFNs L6, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Q486E, I499L and N496D

    Promoter

    CMV

    Available since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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47. pH6HTC_HNRNPA1-Halo

    Plasmid

    #175307

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    HNRNPA1 (HNRNPA1 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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48. M-NMn-VP64

    Plasmid

    #48676

    Purpose

    Mammalian NM-VP64 nuclease-null Cas9 activator expression, human optimized

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Cas9-VP64, nuclease-null
    

    Use

    CRISPR

    Tags

    NLS and VP64

    Expression

    Mutation

    nuclease-null

    Promoter

    CMV

    Available since

    Oct. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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49. pAAV-Sico-Red

    Plasmid

    #84882

    Purpose

    Cre-inducible shRNA

    Depositor

    Eun Mi Hwang

    Article

    Jung et al Exp Neurobiol. 2016 Jun;25(3):120-9. doi

    Insert

    Sico-Red
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    EF1a and CMV

    Available since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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50. pAAV-G12-mTGFB1

    Plasmid

    #219553

    Purpose

    mTGFB1 expression in mammlian cells. pAAV vector. Reporter plasmid.

    Depositor

    Vladislav Verkhusha

    Article

    Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

    Insert

    mTGFB1 (Tgfb1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    12xUAS with TATA-box minimal promoter

    Available since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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