We narrowed to 15,045 results for: NOL

1. pGWB414

   Plasmid

   #74808

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with 3xHA (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   Oct. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. hSTARR-seq_SCP1 vector_blocking 4

   Plasmid

   #99319

   Purpose

   Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

   Depositor

   Alexander Stark

   Article

   Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

   Type

   Empty backbone

   Use

   Hstarr-seq screening vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. FUW-tetO-NFIA

   Plasmid

   #141403

   Purpose

   Inducible Expression of NFIA

   Depositor

   Lorenz Studer

   Article

   Tchieu et al Nat Biotechnol. 2019 Mar;37(3):267-275.

   Insert

   NFIA (NFIA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGWB451

   Plasmid

   #74833

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with G3GFP (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAGK61

   Plasmid

   #228121

   Purpose

   Express node 1467 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

   Depositor

   Seth Shipman

   Article

   Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

   Insert

   Retron node 1467, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG for RT, U6/H1 for ncRNA/gRNA

   Available since

   Dec. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. FUW-tetO-SOX9

   Plasmid

   #141404

   Purpose

   Inducible Expression of SOX9

   Depositor

   Lorenz Studer

   Article

   Tchieu et al Nat Biotechnol. 2019 Mar;37(3):267-275.

   Insert

   SOX9 (SOX9 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGWB404

   Plasmid

   #74798

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with sGFP (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   No Promoter

   Available since

   July 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJL-SaKKH-BE3

   Plasmid

   #85170

   Purpose

   Expresses SaKKH-BE3 in mammalian cells [Edit]

   Depositor

   David Liu

   Article

   Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Insert

   SaKKH-BE3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUC19/NLS-mChe

   Plasmid

   #183162

   Purpose

   Construct for transient expression of cell nucleus-targeted mCherry in plants.

   Depositor

   Qinlong Zhu, Yao-Guang Liu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Insert

   NLS-mCherry
   

   Use

   Tags

   16 aa nuclear localization signal and 16 aa nucl…

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRDB_156

    Plasmid

    #216090

    Purpose

    Cas9 [Sp] CRISPRa targeting CD274, positive control

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    CD274 guide
    

    Use

    CRISPR and Lentiviral; Positive controls

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-eNme2-C.NR

    Plasmid

    #185672

    Purpose

    Expresses nuclease active eNme2-C.NR in mammalian cells

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    eNme2-C.NR nuclease
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    eNme2-C S6P/G33E/A520E/S646R/V696F/R711G/V758I/Y7…

    Promoter

    Available since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRosetta_v2

    Plasmid

    #136477

    Purpose

    Lentiviral GFP Reporter assay for determining appropriate dose of puromycin, blasticidin, and hygromycin

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

    Insert

    HygroR-T2A-BlastR-P2A-PuroR-F2A-EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MMLV-RT(dRH) for Split-PE (pEH50)

    Plasmid

    #190106

    Purpose

    Expresses MMLV-RT (from PE2) with truncated RNAse H domain (dRH) for Split-PE use

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    MMLV-RT(delta RNAseH)
    

    Use

    Tags

    bpNLS

    Expression

    Mammalian

    Mutation

    mutations from RT in PE2 and truncation of RNAse …

    Promoter

    CMV

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGI3EM17

    Plasmid

    #210569

    Purpose

    Reporter gene expression in B. dendrobatidis

    Depositor

    Lillian Fritz-Laylin

    Article

    Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

    Insert

    SpH2Bpro-Hshph-G4S-nanoluc-ScADH1ter
    

    Use

    Luciferase

    Tags

    NanoLuc luciferase

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    SpH2Bpro

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pY2ShHELIX_sgRNA_entry (CJT30)

    Plasmid

    #181781

    Purpose

    Expresses Y2-ShHELIX containing a nicking Y2 I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    Y2-nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y2-nAniI = F13Y, S111Y, K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRDB_118

    Plasmid

    #216089

    Purpose

    Cas9 [Sp] CRISPRa targeting CD47, positive control

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    CD47 guide
    

    Use

    CRISPR and Lentiviral; Positive controls

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pShuttle-CMV-iRFP

    Plasmid

    #31856

    Purpose

    a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging

    Depositor

    Vladislav Verkhusha

    Article

    Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

    Insert

    iRFP
    

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    T2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198…

    Promoter

    CMV

    Available since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. piGECI-N1

    Plasmid

    #160421

    Purpose

    Cytoplasmic expression of a near-infrared genetically encoded calcium indicator iGECI

    Depositor

    Vladislav Verkhusha

    Article

    Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

    Insert

    iGECI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGWB544

    Plasmid

    #74878

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with eCFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGWB444

    Plasmid

    #74830

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with eCFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLVX-Tight-Puro_Sox10

    Plasmid

    #46536

    Purpose

    A lentiviral vector for the expression of mouse Sox10 under doxycyline control.

    Depositor

    Paul Tesar

    Article

    Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

    Insert

    Sox10 (Sox10 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGM1202

    Plasmid

    #69615

    Purpose

    inducible gene expression in Streptomyces, C-terminal His-tag encoding sequence

    Depositor

    Günther Muth

    Article

    Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

    Inserts

    aac
    tsr
    rep
    sso
    TipA promoter
    dso
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGL3 NSE promoter

    Plasmid

    #11606

    Depositor

    Rosalyn Adam

    Article

    Kim et al Endocrinology. 2004 Feb . 145(2):613-9.

    Insert

    NSE promoter (ENO2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLenti_CMV:Flag-GeNL

    Plasmid

    #208841

    Purpose

    Expresses FLAG epitope-tagged GeNL "Green" BRET reporter in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

    Insert

    GeNL Nanolantern
    

    Use

    Lentiviral

    Tags

    1xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRDA_833

    Plasmid

    #216086

    Purpose

    Cas9 [Sp] knockout targeting CD47, positive control

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    CD47 guide
    

    Use

    CRISPR and Lentiviral; Positive controls

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pUC19/Mito-mChe

    Plasmid

    #183167

    Purpose

    Construct for transient expression of mitochondrion-targeted mCherry in plants.

    Depositor

    Qinlong Zhu, Yao-Guang Liu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Insert

    Mito-mCherry
    

    Use

    Tags

    OsCOX11, Oryza sativa cytochrome c oxidase assemb…

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMKO.1 puro GFP shRNA

    Plasmid

    #10675

    Purpose

    Negative control vector for pMKO.1 puro (Addgene plasmid #8452); Contains shRNA against GFP.

    Depositor

    William Hahn

    Article

    Masutomi et al Proc Natl Acad Sci U S A. 2005 Jun 7. 10

    Insert

    GFP shRNA
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCTCON2 Aga2P-HRP

    Plasmid

    #73152

    Purpose

    Full-length horseradish peroxidase (HRP) fused to the C-terminus of the yeast mating protein Aga2P. Galactose-inducible expression.

    Depositor

    Alice Ting

    Article

    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

    Insert

    Aga2P-HRP
    

    Use

    Tags

    HA tag and myc tag

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGWB513

    Plasmid

    #74855

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with 3xHA (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMKO.1 puro hTERT shRNA

    Plasmid

    #10688

    Depositor

    William Hahn

    Article

    Masutomi et al Proc Natl Acad Sci U S A. 2005 Jun 7. 10

    Insert

    hTERT shRNA (TERT Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. gRNA NIA Null

    Plasmid

    #198728

    Purpose

    Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Neither gRNA is fused to a mobility signal and are therefore non-mobile.

    Depositor

    Friedrich Kragler

    Article

    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    AtNIA1 gRNAs
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pU6-26, pU6-29

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCIpA102-G-HLA-A2_GFP

    Plasmid

    #85162

    Purpose

    for mRNA expression of HLA.A2-EGFP

    Depositor

    Sébastien Walchli, Tone Fredsvik Gregers

    Article

    Walchli et al Eur J Immunol. 2014 Mar;44(3):774-84. do

    Insert

    HLA.A2 (HLA-A Human)
    

    Use

    Mrna expression

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pJBL703

    Plasmid

    #140373

    Purpose

    Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 2BP dowsntream from the promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-2BP-tetO-3WJdB-T
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pEK19

    Plasmid

    #210578

    Purpose

    Reporter gene expression in B. dendrobatidis

    Depositor

    Lillian Fritz-Laylin

    Article

    Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

    Insert

    BdBIP1pro-Hshph-G4S-nanoluc-ScADH1ter
    

    Use

    Luciferase

    Tags

    NanoLuc luciferase

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    BdBIP1pro

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCellFree_G03 CIRBP

    Plasmid

    #67055

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    CIRBP (CIRBP Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

36. pGWB415

    Plasmid

    #74809

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with 3xHA (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pR26 CAG/BFP Dest

    Plasmid

    #74282

    Purpose

    Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, BFP, for gateway cloning

    Depositor

    Ralf Kuehn

    Article

    Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

    Insert

    Rosa26 5-homology region
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3 IKKe flag

    Plasmid

    #26201

    Depositor

    Tom Maniatis

    Article

    Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

    Insert

    I-kappa-B kinase epsilon (IKBKE Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGWB550

    Plasmid

    #74880

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with G3GFP (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pENTR-HLA-A2_GFP

    Plasmid

    #85161

    Purpose

    HLA.A2_GFP fusion with 2 glycines in between

    Depositor

    Sébastien Walchli, Tone Fredsvik Gregers

    Article

    Walchli et al Eur J Immunol. 2014 Mar;44(3):774-84. do

    Insert

    HLA.A2 (HLA-A Human)
    

    Use

    Gateway entry vector

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. HBT-pcoCas9

    Plasmid

    #52254

    Purpose

    transient expression of pcoCas9 gene in plant cells

    Depositor

    Jen Sheen

    Article

    Li et al Nat Biotechnol. 2013 Aug;31(8):688-91. d

    Insert

    pcoCas9
    

    Use

    CRISPR; Plant expression

    Tags

    FLAG and NLS

    Expression

    Mutation

    Promoter

    hybrid constitutive promoter 35SPPDK

    Available since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pU6-Sp-gRNA-AAVS1_B3903c_attP

    Plasmid

    #182144

    Purpose

    To insert attP attachment site at AAVS1 in human cells via twinPE

    Depositor

    David Liu

    Article

    Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

    Insert

    AAVS1_B3903c_attP pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pU6-Sp-gRNA-AAVS1_A3786c_attP

    Plasmid

    #182143

    Purpose

    To insert attP attachment site at AAVS1 in human cells via twinPE

    Depositor

    David Liu

    Article

    Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

    Insert

    A3786c-attP pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pLVX-Tight-Puro_MYRF

    Plasmid

    #64843

    Purpose

    A lentiviral vector for the expression of mouse MYRF under doxycyline control.

    Depositor

    Paul Tesar

    Article

    Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

    Insert

    MYRF (Myrf Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pXPR_206

    Plasmid

    #96920

    Purpose

    for SaCas9 knockout, lentiviral expression of S. aureus Cas9 (SauCas9) and gRNA scaffold

    Depositor

    David Root, John Doench

    Article

    Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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46. pGWB535

    Plasmid

    #74873

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with LUC (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    No Promoter

    Available since

    Oct. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pv6_CBX1_2xChromo

    Plasmid

    #179393

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1_2xChromo

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    CBX1_2xChromo
    

    Use

    Tags

    AviTag and EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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48. pTXB1.Tat(48-57)-Cre recombinase

    Plasmid

    #165042

    Purpose

    Expression of Tat(48-57) conjugated Cre recombinase for protein purification (Intein-chitin binding domain fussion)

    Depositor

    Dokyun Na

    Article

    Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

    Insert

    Cre recombinase
    

    Use

    Tags

    Mxe Intein-Chitin binding domain and Tat(48-57)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pBK-VQR-BE3

    Plasmid

    #85171

    Purpose

    Expresses VQR-BE3 in mammalian cells

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    VQR-BE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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50. pGWB504

    Plasmid

    #74846

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with sGFP (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    No Promoter

    Available since

    Oct. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge