8,736 results

1. pCMV-dCas13-M3M14nes

   Plasmid

   #155367

   Purpose

   Targeted m6A RNA methylation in mammalian cells

   Depositor

   David Liu

   Article

   Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

   Insert

   dCas13-M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   PspCas13b Δ984-1090 H133A

   Promoter

   Available since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRK5-EGFP-Tau AP

   Plasmid

   #46905

   Purpose

   expresses EGFP tagged Tau AP in mammalian cells

   Depositor

   Karen Ashe

   Article

   Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

   Insert

   Tau AP (MAPT Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   all 14 S/P or T/P amino acid residues (T111, T153…

   Promoter

   CMV

   Available since

   July 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. Klotho (membrane)

   Plasmid

   #17712

   Depositor

   Hal Dietz

   Article

   Arking et al Proc Natl Acad Sci U S A. 2002 Jan 22. 9

   Insert

   Klotho (KL Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   P101S

   Promoter

   Available since

   April 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. Gq-CASE

   Plasmid

   #168125

   Purpose

   Encodes a BRET-based activity sensor for the heterotrimeric G protein Gq. Composed of the subunits G alpha q (GNAQ) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

   Depositor

   Gunnar Schulte

   Article

   Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

   Insert

   GNAQ, GNB3, GNG9 (GNAQ, GNB3, GNGT2 Human)
   

   Use

   Luciferase

   Tags

   cpVenus on GNG9

   Expression

   Mammalian

   Mutation

   NLuc is inserted at F124/E125 within GNAQ

   Promoter

   Available since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST53-LRRK2-R1441C

   Plasmid

   #25046

   Depositor

   Mark Cookson

   Article

   Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Arginine 1441 mutated to Cysteine

   Promoter

   Available since

   June 17, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pOSIP-KO (KanR, 186)

   Plasmid

   #45985

   Depositor

   Drew Endy, Keith Shearwin

   Article

   St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. wt dynamin 2 pEGFP

   Plasmid

   #34686

   Depositor

   Sandra Schmid

   Insert

   dynamin 2 (Dnm2 Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   D406G, V664A, H692R and Q758R

   Promoter

   CMV

   Available since

   Feb. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Mfn2-YFP

   Plasmid

   #28010

   Depositor

   Richard Youle

   Article

   Karbowski et al J Cell Biol. 2002 Dec 23. 159(6):931-8.

   Insert

   mitofusin 2 (MFN2 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   silent S508S

   Promoter

   Available since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDH-puro-EWS-FLI1

   Plasmid

   #102813

   Purpose

   Overexpress EWS-FLI1. 3rd generation lentiviral vector.

   Depositor

   Jialiang Wang

   Article

   Loganathan et al Oncotarget. 2016 Jul 12;7(28):43504-4351

   Insert

   EWS-FLI1 (EWSR1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. p1321 HPV-16 E6/E7

    Plasmid

    #8641

    Depositor

    Peter Howley

    Article

    Münger et al J Virol. 1989 Oct . 63(10):4417-21.

    Insert

    HPV-16 E6/E7 (E7, E6 Human papillomavirus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Amph1-pmCherryN1

    Plasmid

    #27692

    Depositor

    Christien Merrifield

    Article

    Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

    Insert

    Amphiphysin 1 (Amph Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFA6a-6xGLY-FLAG-kanMX6

    Plasmid

    #20751

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEGFPN3-Sstr3

    Plasmid

    #35623

    Depositor

    Kirk Mykytyn

    Article

    Berbari et al Mol Biol Cell. 2008 Apr;19(4):1540-7. Ep

    Insert

    Sstr3 (Sstr3 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-Neo_CAG-Cas9

    Plasmid

    #86698

    Purpose

    Cas9 expression vector for targeting to the AAVS1 locus

    Depositor

    Ludovic Vallier

    Article

    Bertero et al Development. 2016 Dec 1;143(23):4405-441

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCag

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. p2lox-cAMPr

    Plasmid

    #99143

    Purpose

    Expresses cAMPr in mammalian cells

    Depositor

    Justin Blau

    Article

    Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/

    Insert

    cAMPr
    

    Use

    Cre/Lox

    Tags

    cpGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. Cas9m4-VP64

    Plasmid

    #47319

    Purpose

    Cas9m4 Activator

    Depositor

    George Church

    Article

    Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

    Insert

    Cas9m4-VP64
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTALYM3B15

    Plasmid

    #47878

    Purpose

    TALE-mClover against mouse major satellite sequence

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Miyanari et al Nat Struct Mol Biol. 2013 Oct 6. doi: 10

    Insert

    TALE-mClover
    

    Use

    TALEN

    Tags

    mClover

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP-HSPA8

    Plasmid

    #121161

    Purpose

    Mammalian expression of GFP tagged HSPA8

    Depositor

    Christine Mayr

    Article

    Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

    Insert

    HSPA8 (HSPA8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-Ef1a-DIO-mCherry-WPRE-pA

    Plasmid

    #37083

    Purpose

    Cre-dependent expression of mCherry

    Depositor

    Bernardo Sabatini

    Article

    Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

    Insert

    mCherry
    

    Use

    AAV and Cre/Lox; Cre-on

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    July 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLENTI6.3/AR-GC-E2325

    Plasmid

    #85128

    Purpose

    exogenous AR expression

    Depositor

    Karl-Henning Kalland

    Article

    Olsen et al BMC Cancer. 2016 Jul 4;16:377. doi: 10.1

    Insert

    androgen receptor (AR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge