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pSIN4-EF1a-TAL1-IRES-Puro

Plasmid

#61065

Purpose

lentiviral vector for TAL1

Depositor

Igor Slukvin

Article

Elcheva et al Nat Commun. 2014 Jul 14;5:4372. doi: 10.

Insert

TAL1 (TAL1 Human)

Use

Lentiviral

Tags

FLAG

Promoter

EF1alpha

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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T7-CMVtrans-FFLuc-polyA

Plasmid

#101156

Purpose

Template for T7 RNAP production of RNA transcript for expression of firefly luciferase in a eukaryotic mammalian cell free lysate.

Depositor

Marcel Bruchez

Article

Canady et al J Am Chem Soc. 2015 Aug 19;137(32):10268

Insert

Firefly luciferase

Use

Luciferase

Promoter

T7 promoter

Available Since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-Bcatfl

Plasmid

#24193

Depositor

David Rimm

Insert

beta-catenin (CTNNB1 Human)

Tags

GST

Expression

Bacterial

Available Since

March 9, 2010

Availability

Academic Institutions and Nonprofits only
AAV-CAG-ChR2-GFP

Plasmid

#26929

Purpose

AAV-mediated expression of ChR2-GFP for neuronal excitation

Depositor

Edward Boyden

Article

Boyden et al Nat Neurosci. 2005 Sep . 8(9):1263-8.

Insert

ChR2

Use

AAV; Adeno-associated virus

Tags

GFP

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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mCherry-Parkin

Plasmid

#23956

Purpose

Mammalian expression of human Park2 fused to mCherry

Depositor

Richard Youle

Article

Narendra et al J Cell Biol. 2008 Dec 1. 183(5):795-803.

Insert

Park2 (PRKN Human)

Tags

mCherry

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only

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FH-TET1-pEF

Plasmid

#49792

Purpose

This plasmid expresses the full-length human TET1 gene with a Flag and HA tag.

Depositor

Anjana Rao

Article

Tahiliani et al Science. 2009 May 15;324(5929):930-5. Ep

Insert

Tet methylcytosine dioxygenase 1 (TET1 Human)

Tags

Flag and HA

Expression

Mammalian

Promoter

EF-1alpha

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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MSCV-XZ066-EGFRvIII

Plasmid

#20737

Depositor

Alonzo Ross

Article

Li et al Neuro Oncol. 2009 Feb . 11(1):9-21.

Insert

EGFRvIII (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

EGFR with a 267 amino acid deletion in the extrac…

Available Since

March 31, 2009

Availability

Academic Institutions and Nonprofits only

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AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR

Plasmid

#60226

Purpose

Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

Depositor

Feng Zhang

Article

Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

Inserts

sgRNA
Renilla luciferase
Cre recombinase

Use

AAV, CRISPR, Cre/Lox, Luciferase, and Mouse Targe…

Tags

Cre-HA and Rluc-P2A

Expression

Mammalian

Promoter

EFS and U6

Available Since

Oct. 9, 2014

Availability

Academic Institutions and Nonprofits only

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XBP-1 2: pFLAG.XBP1p.CMV2

Plasmid

#21833

Depositor

David Ron

Article

Calfon et al Nature. 2002 Jan 3. 415(6867):92-6.

Insert

XBP-1 processed/spliced (Xbp1 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 8, 2009

Availability

Academic Institutions and Nonprofits only

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pX330S-2-PITCh

Plasmid

#63670

Purpose

Expresses Cas9 nuclease and the PITCh-gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pMK345 (Hygro-P2A-mAID-mClover)

Plasmid

#121179

Purpose

N-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

Hygro-P2A-mAID-mClover

Use

CRISPR

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-Flpe:GFP

Plasmid

#13788

Purpose

Mammalian expression of enhanced form of Flp recombinase fused to GFP

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

Flpe-GFP fusion protein

Use

Flp/frt

Tags

GFP

Expression

Mammalian

Promoter

CAG

Available Since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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pTRE-TIGHT miR-1 (aka:pTRE-m1d)

Plasmid

#14896

Depositor

David Bartel

Article

Lewis et al Cell. 2003 Dec 26. 115(7):787-98.

Insert

miR-1

Expression

Mammalian

Available Since

April 27, 2007

Availability

Academic Institutions and Nonprofits only

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pLV_hEF1a_rtTA3

Plasmid

#61472

Purpose

3rd generation lentiviral vector; constitutive expression of rtTA3 (TetON system)

Depositor

Ron Weiss

Article

Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

Insert

rtTA3

Use

Lentiviral

Promoter

hEF1a

Available Since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pAct-Cas9

Plasmid

#62209

Purpose

Expresses Cas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous genome engineering in Drosophila melanogaster.

Depositor

Simon Bullock

Article

Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

Insert

cas9

Expression

Insect

Promoter

act5C

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pLX307 Luciferase

Plasmid

#117734

Purpose

Open reading frame vector encoding Luciferase

Depositor

William Hahn

Article

Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

Insert

firefly luciferase

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pBad-EBFP2

Plasmid

#14891

Purpose

Bacterial expression of EBFP2 blue fluorescent protein

Depositor

Robert Campbell

Article

Ai et al Biochemistry. 2007 May 22;46(20):5904-10

Has Service

Cloning Grade DNA

Insert

EBFP2

Tags

His

Expression

Bacterial

Mutation

The fluorescent protein was inserted between XhoI…

Available Since

May 15, 2007

Availability

Academic Institutions and Nonprofits only

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pJRH-1180 U6-reci Gag-pol v2

Plasmid

#201915

Purpose

Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

Gag-pol

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-SMN

Plasmid

#37057

Depositor

Greg Matera

Article

Shpargel et al Proc Natl Acad Sci U S A. 2005 Nov 29;10

Insert

Human SMN1 (SMN1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pSIN4-EF2-O2S

Plasmid

#21162

Depositor

James Thomson

Article

Yu et al Science. 2009 Mar 26.

Insert

Oct4, Sox2 (SOX2, POU5F1 Human)

Use

Lentiviral; Reprogramming

Available Since

June 9, 2009

Availability

Academic Institutions and Nonprofits only

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