We narrowed to 9,310 results for: Coli
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pTU2S-b (p15A origin)
Plasmid#74089PurposeSecondary module (BpiI) site in pTU2-a backbone with p15A (low-copy) origin of replicationDepositorInsertGolden Gate destination vector - Level 2, 3 TU's with secondary module and p15A origin
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterNoneAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGK:TRL-EFS:iRFP670
Plasmid#68475PurposeLentiviral plasmid expressing tTR-KRAB-rtTA3-Luc cassette and iRFP670DepositorInsertstTR-KRAB-2A-rtTA3-2A-Luciferase
iRFP670
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterEFS and pGKAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp15-DARPinOFF7
Plasmid#182399PurposeBacterial expression of Vesicle Nucleating peptide15-DARPinOFF7 fusionDepositorInsertVNp15-DARPinOFF7
UseTagsVesicle Nucleating peptide 15 (VNp15)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2SCb
Plasmid#59385PurposeTemplate plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a modified chloramphenicol resistance gene.DepositorInsertTT-ISceI-cat2
UseTemplateTagsExpressionMutationThe 3’-end of the chloramphenicol resistance gene…PromoterAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
BirA*-HA-NES
Plasmid#214473PurposeLentiviral vector for expression in mammalian cells of the proximity ligase BirA(R118G) with a C-terminal HA tag followed by a nuclear exclusion sequence.DepositorInsertBirA(R118G)
UseLentiviral; GatewayTagsHA tag and NES (Nuclear Exclusion Sequence)ExpressionMammalianMutationR118GPromoterEF-1aAvailable sinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBEST_OR2-OR1-Pr-UTR1_ClpP-T500
Plasmid#153302PurposeExpresses ClpP protease in the cell-free TX-TL systemDepositorInsertclpP (clpP Escherichia coli)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTS2356_Tier3(PB)-PuroR
Plasmid#169655PurposeTier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-PuroR-pA-3'ITR)DepositorInsertPRPBSA-driven PuroR expression
UseTagsExpressionMammalianMutationPromoterPRPBSAAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-hPDI
Plasmid#60555Purposeenhance solubility and correct disulfide bond-formation of fused protein expressed in E.coliDepositorTypeEmpty backboneUseTagsHis6-hPDIExpressionBacterialMutationPromoterAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-baPrs-hdNadV
Plasmid#91950PurposepET28a-baPrs-hdNadV is a bicistronic vector designed for the simultaneous expression of PRS from Bacillus amyloliquefaciens and NadV from Haemophilus ducreyiDepositorInsertsPutative Nicotinamide Phosphoribosyl Transferase (nadV Haemophilus ducreyi, strain: ATCC 27722)
Phosphoribosyl Pyrophosphate Synthetases
UseTagsExpressionBacterialMutationchanged Leucine 135 to IsoleucinePromoterT7Available sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
prCAG-RBS"m1"-sfGFP
Plasmid#191400PurposeExpression of 47 rCAG repeats fused to a translational unit over-expressing superfolder GFP using a strong RBS. The RBS contains mutation (m1)DepositorInsert47xCAG RBS"m1"-sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLtet0-1Available sinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
Ad-CBR (GFP/cAPC)
Plasmid#16576DepositorInsertcAPC (APC Human)
UseAdenoviralTagsEGFPExpressionMammalianMutationResidues 958-2076PromoterAvailable sinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
CAP Z alpha 1 beta 1 CP
Plasmid#13451DepositorInsertcapping protein alpha 1 beta 1 (chicken) (CAPZB Chicken)
UseTagsExpressionBacterialMutationnonePromoterAvailable sinceDec. 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCph8
Plasmid#22869DepositorInsertpCph8
UseSynthetic BiologyTagsExpressionBacterialMutationFirst 517 amino acids of Cph1 and the last 229 am…PromoterAvailable sinceJune 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDEST-hPDIb′a′
Plasmid#60556Purposeenhance solubility and correct disulfide bond-formation of fused protein expressed in E.coliDepositorTypeEmpty backboneUseTagsHis6-hPDIb'a' tagExpressionBacterialMutationPromoterAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSKA397
Plasmid#80380Purposemini-Tn7 delivery plasmid with metE under the cpcG2Δ59 promoter.DepositorInsertscat
metE
UseSynthetic Biology; Mini-tn7 delivery vectorTagsExpressionBacterialMutationPromotercat promoter and cpcG2Δ59Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSET-TEV-12His
Plasmid#137032Purposeempty vector; C-terminal TEV-protease-cleavable His12; T7 promoter; BseRI cloning sites (e.g., In-Fusion compatible)DepositorTypeEmpty backboneUseTagsHis12 (TEV protease cleavable)ExpressionBacterialMutationPromoterT7Available sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETM6-At4CL-CmCHS-CmCHI
Plasmid#73395PurposeExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivoDepositorInsertsAt4CL
CmCHS
CmCHI
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSC201
Plasmid#129387PurposeA variant of the pSCrhaB2 vector with the oriR6K and mob genes from pGPΩ-TP. Used for homologous recombination of the rhamnose-inducible system into the chromosome of Burkholderia cenocepacia K56-2.DepositorInsertoriR6K and oriT
UseTagsExpressionBacterialMutationN/APromoterN/AAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRG/III-hs-MBP-A2aTr316-H10
Plasmid#121683PurposeBacterial expression of adenosine A2a receptor fusion proteinDepositorInsertadenosine A2a receptor (ADORA2A Human)
UseTagsHis10ExpressionBacterialMutationPromoterlacAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLaNGo11-1
Plasmid#126053PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cellDepositorInsertschloroplastic FNR transit peptide
ccdB cassette (BsaI)
GFP11
UseBinaryTagsGFP1-10ExpressionPlantMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
aav-CAG-FLEX-tdNfsB(F124W)-mCherry (eNTR)
Plasmid#113762PurposeAAV-mediated conditional expression of bacterial tdNfsB-mCherry (eNTR) under the CAG promoterDepositorInserttdNfsB(F124W)-mCherry
UseAAV and Cre/LoxTagsmCherryExpressionMammalianMutationF124WPromoterCAGAvailable sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOE5751.1
Plasmid#135083PurposepBR322-based L-rhamnose-inducible vector.DepositorInsertP-rhaP-BAD-His tag-eGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETM6-Pc4CL-CmCHS-MsCHI
Plasmid#73398PurposeExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivoDepositorInsertsPc4CL
CmCHS
MsCHI
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTU2S-b (colE1 origin)
Plasmid#74092PurposeSecondary module (BpiI) site in pTU2-a backbone with colE1 (medium-copy) origin of replicationDepositorInsertGolden Gate destination vector - Level 2, 3 TU's with secondary module and colE1 origin
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterNoneAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETM6-At4CL-PhCHS-CmCHI
Plasmid#73393PurposeExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivoDepositorInsertsAt4CL
PhCHS
CmCHI
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
E0030 (YFP)_CD
Plasmid#66031PurposeMoClo Basic Part: CDS - Fluorescent protein. Yellow. [C:E0030:D]DepositorInsertFluorescent reporter - YFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
E1010m (RFP)_CD
Plasmid#66033PurposeMoClo Basic Part: CDS - Fluorescent protein. Red. Modified from Bba_E1010 to fix illegal sites. [C:E1010m:D]DepositorInsertFluorescent reporter - RFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
aav-SYN-FLEX-tdNfsB(F124W)-mCherry (eNTR)
Plasmid#113763PurposeAAV-mediated conditional expression of bacterial tdNfsB-mCherry (eNTR) under the SYN promoterDepositorInserttdNfsB(F124W)-mCherry
UseAAV and Cre/LoxTagsmCherryExpressionMammalianMutationF124WPromoterSynapsinAvailable sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSLS.546
Plasmid#184963PurposeTest effect of a1/a2 length on editing priB using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, priB G1069A, a1/a2 length: 22
UseTagsExpressionBacterialMutationpriB donor G1069A, a1/a2 length extended to 22 bpPromoterT7/lacAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only
