We narrowed to 22,946 results for: Tes

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pSIF-H1-hpolb-copGFP

Plasmid

#23256

Depositor

Robert Sobol

Article

Tang et al Mol Cancer Res. 2010 Jan;8(1):67-79. doi

Insert

DNA polymerase beta shRNA

Use

Lentiviral and RNAi

Tags

GFP

Available Since

May 27, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3 Glt1a

Plasmid

#98705

Purpose

mammalian expression of Glt1a

Depositor

Paul Rosenberg

Article

Chen et al J Neurosci. 2002 Mar 15;22(6):2142-52.

Insert

Glt1a (Slc1a2 Rat)

Expression

Mammalian

Mutation

I288V (please see depositor comments below)

Promoter

CMV

Available Since

July 17, 2017

Availability

Academic Institutions and Nonprofits only

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ATF4 13: 5’ATF4.uORF2AUA.GFP

Plasmid

#21860

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

AUG codon of uORF2 was converted to AUA

Available Since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag2B Flag BimEL(TA)

Plasmid

#23093

Depositor

Roger Davis

Article

Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

Insert

Bim EL (TA) (Bcl2l11 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Changed Threonine 112 to Alanine

Available Since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only
pTol2-HybridBarcode

Plasmid

#174037

Purpose

For Tol2 transgenesis of expressed barcode containing SpyCas9, SauCas9, and LbCas12a target sites

Depositor

James Gagnon

Article

Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

Insert

CRISPR target sites

Use

Tol2 transgenesis

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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Keratin 8 S431A

Plasmid

#18058

Depositor

M Bishr Omary

Article

Ku et al J Biol Chem. 2002 Mar 29. 277(13):10775-

Insert

Keratin 8 (KRT8 Human)

Expression

Mammalian

Mutation

S431A

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
pfTRAP

Plasmid

#50048

Purpose

Expresses plasmodium falciparum TRAP adhesion domains (26-299) in mammalian cells

Depositor

Timothy Springer

Article

Song et al Proc Natl Acad Sci U S A. 2012 Dec 26;10

Insert

plamodium falciparum TRAP (TRAP Synthetic)

Tags

6Xhis tag

Expression

Mammalian

Mutation

C55G, N132S

Promoter

CMV

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only
pfTRAP(VWA)

Plasmid

#50049

Purpose

Expresses plasmodium falciparum TRAP VWA domain (41-240) in mammalian cells

Depositor

Timothy Springer

Article

Song et al Proc Natl Acad Sci U S A. 2012 Dec 26;10

Insert

pfTRAP(vwa) (TRAP Synthetic)

Tags

6Xhis tag

Expression

Mammalian

Mutation

C55G, N132S

Promoter

CMV

Available Since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
pRK5-TGF beta type I receptor (mL45)

Plasmid

#34701

Depositor

Ying Zhang

Article

Yu et al EMBO J. 2002 Jul 15;21(14):3749-59.

Insert

TGF beta type I receptor (Tgfbr1 Rat)

Tags

Flag

Expression

Mammalian

Mutation

N265A, D267A, N268A

Promoter

CMV

Available Since

June 22, 2012

Availability

Academic Institutions and Nonprofits only
pET21a-mAIM2-deltaPYD

Plasmid

#51535

Purpose

Express His6-tagged HIN domain of mAIM2 in bacteria

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

Aim2 (Aim2 Mouse)

Tags

His6 and T7

Expression

Bacterial

Promoter

lac operator

Available Since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pMX155

Plasmid

#29481

Depositor

Linda Wordeman

Article

Moore et al J Cell Biol. 2005 May 9. 169(3):391-7.

Insert

Kif2A-alpha (Kif2a Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

E140K

Available Since

April 7, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA6-5'UTR(mtIF3)-mtIF3-Dendra2-3'UTR(mtIF3)

Plasmid

#182370

Purpose

Dendra2-based translation reporter

Depositor

Chunghun Lim

Article

Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

Insert

mtIF3 (Mtif3 Mouse)

Tags

Pal-Dendra2

Expression

Mammalian

Promoter

CMV promoter

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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tau exon 9–12 FTDP-17 N279K splicing minigene

Plasmid

#122967

Purpose

expresses circular tau RNAs with the FTDP-17 N279K

Depositor

Stefan Stamm

Article

Welden et al Biochim Biophys Acta Mol Basis Dis. 2018

Insert

maps

Expression

Mammalian

Mutation

FTDP-17 mutation

Available Since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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2-Luc

Plasmid

#21724

Depositor

Wafik El-Deiry

Article

Zeng et al Nat Genet. 1997 Jan . 15(1):78-82.

Insert

p21 promoter deletion mutant (CDKN1A Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only
F5A deltaCT S6K1 MSCV puro

Plasmid

#8464

Depositor

David Sabatini

Article

Ali et al J Biol Chem 2005 Apr 4;.

Insert

f5a delta ctp70 S6K1 (Rps6kb1 Rat)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

deletion of 104 c-terminal amino acids mutation …

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pFastBac-His-Flag-Tipin

Plasmid

#22890

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

Insert

Tipin (TIPIN Human)

Tags

Flag- and His

Expression

Insect

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pCMV-FKBP-mCherry-Vav2cat

Plasmid

#178865

Purpose

Mammalian expression of Vav2(DH-PH-CRD) fused to FKBP-mCherry

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

FKBP-mCherry-Vav2(DH-PH-CRD) (Vav2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pEF-HA-Net1

Plasmid

#28264

Depositor

Jeffrey Frost

Article

Carr et al J Biol Chem. 2009 Sep 4. 284(36):24269-8

Insert

Net1 (Net1 Mouse)

Tags

2x HA

Expression

Mammalian

Available Since

April 11, 2011

Availability

Academic Institutions and Nonprofits only
pSLIK 3XFLAG-MKK4-EE-NLS hygro

Plasmid

#87779

Purpose

Inducible expression of constitutively active mutant MKK4 with a C-terminal NLS sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

MKK4

Use

Lentiviral

Tags

3xFLAG and NLS

Expression

Mammalian

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only
pIhh-5A9-LUC

Plasmid

#46318

Depositor

Xiangli Yang

Article

Wang et al Development. 2009 Dec;136(24):4143-53. d

Insert

5 copies of A9 sequence from Ihh promoter

Tags

luciferase

Expression

Mammalian

Promoter

mouse osteocalcin gene 2 promoter

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
pALPS_3'LTR GFP@gag start SFFV rev

Plasmid

#101339

Purpose

LTR drives GFP and internal SFFV promoter drives codon optimized rev

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

GFP, rev

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET:GACG:GFP

Plasmid

#60735

Purpose

A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GACG' between -11 and -8.

Depositor

Matthew Bennett

Article

Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-Tag2B Flag BimEL(TD)

Plasmid

#23092

Depositor

Roger Davis

Article

Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

Insert

Bim EL (TD) (Bcl2l11 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Changed Threonine 112 to Aspartate

Available Since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-hAIM2deltPYD-T7

Plasmid

#51539

Purpose

Express T7-tagged HIN domain of hAIM2 in mammalian cells

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

AIM2 (AIM2 Human)

Tags

T7

Expression

Mammalian

Mutation

deletion of pyrin domain

Promoter

CMV

Available Since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

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CAG-RARa-VP16

Plasmid

#185559

Purpose

Ubiquitous expression of constitutively active Retinoic Acid Receptor alpha

Depositor

Connie Cepko

Article

Amamoto et al Elife. 2022 Mar 22;11. pii: 76389. doi:

Insert

RARa-VP16 (Rara Mouse)

Tags

VP16

Expression

Mammalian

Promoter

CAG

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1-mSNX27 RNAi-resistant

Plasmid

#163620

Purpose

mouse SNX27 cDNA with point mutation to resist siRNA against SNX27 C-terminally tagged with GFP

Depositor

Josef Kittler

Article

Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

Insert

mouse SNX27 RNAi-resistant (Snx27 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

RNAi resistance

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pET:GCAT:GFP

Plasmid

#60736

Purpose

A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GCAT' between -11 and -8.

Depositor

Matthew Bennett

Article

Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21a-mAIM2-GST

Plasmid

#51533

Purpose

Express GST-tagged mAIM2 in bacteria

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

Aim2 (Aim2 Mouse)

Tags

GST

Expression

Bacterial

Promoter

lac operator

Available Since

March 11, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH3043

Plasmid

#175284

Purpose

Pdpy-30 DAF-16a unc-54 3' UTR C.elegans pan-tissue expression of isoform a of DAF-16 fused to GFP

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

daf-16a mini-gene (daf-16 Nematode)

Expression

Worm

Promoter

Pdpy-30

Available Since

Nov. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
ATF4 12: 5’ATF4.uORF1AUA.GFP

Plasmid

#21859

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

AUG codon of uORF1 was converted to AUA

Available Since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pHA843 - hrp-1p::hrp-1HsLCdeltaLC

Plasmid

#139200

Purpose

Expresses hrp-1p::hrp-1HsLCdeltaLC::hrp-1 3'UTR

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Expression

Worm

Mutation

stop at beginning of last exon

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
SERA5-bio-His

Plasmid

#50817

Purpose

Expresses enzymatically monobiotinylated full length SERA5

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

SERA5

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pSicoR-mCh-Chd1i1

Plasmid

#21904

Depositor

Miguel Ramalho-Santos

Article

Gaspar-Maia et al Nature. 2009 Aug 13;460(7257):863-8. doi

Insert

Chd1-i1

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 30, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
PcDNA-DEST47-CypB-Iso C-term GFP

Plasmid

#36130

Depositor

Patrick Van Oostveldt

Article

Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

Insert

cyclophilin B (PPIB Human)

Tags

GFP

Expression

Mammalian

Mutation

contains amino acids 47-204 of cyclophilin B

Promoter

CMV

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
pGL3-PIM2

Plasmid

#51848

Purpose

promoter region (-550;0) of PIM2

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

PIM2 (PIM2 Human)

Use

Luciferase; Reporter vector

Available Since

July 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGE-attBCC-FRT-mW (pTL788)

Plasmid

#115159

Purpose

Integration vector for phiC31-mediated transgenesis using a genomic attP-CC site. Contains an attB-CC site, the visible GMR-white flanked by a FRT site for FLP-mediated removal.

Depositor

Tobias Langenhan

Article

Blanco-Redondo et al G3 (Bethesda). 2018 Jul 31. pii: g3.118.

Insert

attB-CC FRT

Expression

Insect

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
PcDNA-DEST47-CypB-Golgi C-term GFP

Plasmid

#36128

Depositor

Patrick Van Oostveldt

Article

Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

Insert

cyclophilin B (PPIB Human)

Tags

GFP

Expression

Mammalian

Mutation

contains amino acids 1-211 of cyclophilin B

Promoter

CMV

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
pHR-MCS-YFP-NLS

Plasmid

#145274

Purpose

Mammalian Expression of Nuclear YFP with N-terminal MCS and SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

MCS and YFP

Expression

Mammalian

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHMWGFPR

Plasmid

#64396

Purpose

High molecular weight glutenin gene promoter driving GFP expression

Depositor

Claudia Vickers

Article

Vickers et al Plant Mol Biol. 2006 Sep;62(1-2):195-214

Insert

High molecular weight glutenin gene promoter

Tags

GFP

Expression

Plant

Promoter

High molecular weight glutenin gene promoter

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only
pCAGGS-PB1-MontiRed-LOV2

Plasmid

#178872

Purpose

Mammalian expression of PB1-MontiRed-LOV2

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

PB1-MontiRed-LOV2

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
SEPT5 Mut(S327A)

Plasmid

#27274

Depositor

Harish Pant

Article

Amin et al J Neurosci. 2008 Apr 2. 28(14):3631-43.

Insert

Septin 5 (SEPTIN5 Human)

Tags

Myc (MASMQKLISEEDL)

Expression

Mammalian

Mutation

S327A

Available Since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only
pLentiCRISPR V2 sgACO1_1

Plasmid

#169885

Purpose

Disrupt ACO1

Depositor

Richard Possemato

Article

Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

Insert

sgRNA targeting ACO1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR V2 sgACO1_2

Plasmid

#169892

Purpose

Disrput ACO1

Depositor

Richard Possemato

Article

Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

Insert

sgRNA targeting ACO1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH2947

Plasmid

#132362

Purpose

Prgef-1 INS-2::GFP unc-54 3' UTR C.elegans pan-neural expression of INS-2::GFP

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

INS-2 (ins-2 Nematode)

Tags

GFP

Expression

Worm

Promoter

Prgef-1

Available Since

Oct. 7, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
PcDNA-DEST53-CypB-Golgi N-term GFP

Plasmid

#36127

Depositor

Patrick Van Oostveldt

Article

Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

Insert

cyclophilin B (PPIB Human)

Tags

GFP

Expression

Mammalian

Mutation

contains amino acids 1-211 of cyclophilin B

Promoter

CMV

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3 Glt1b

Plasmid

#98706

Purpose

mammalian expression of Glt1b

Depositor

Paul Rosenberg

Article

Chen et al J Neurosci. 2002 Mar 15;22(6):2142-52.

Insert

Glt1b (Slc1a2 Rat)

Expression

Mammalian

Mutation

I348V and N365Y (please see depositor comments se…

Promoter

CMV

Available Since

July 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLJM1-zeo-2xStrep-BFLF1

Plasmid

#162661

Purpose

Lentiviral vector for stable expression of N-terminal 2xStrep-tagged BFLF1 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

BFLF1

Use

Lentiviral

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
997 pSG5L HA PTEN T382A

Plasmid

#10757

Depositor

William Sellers

Article

Vazquez et al Mol Cell Biol. 2000 Jul . 20(14):5010-8.

Insert

PTEN (PTEN Human)

Tags

HA

Expression

Mammalian

Mutation

T382A

Available Since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only
pWZL blast goosecoid

Plasmid

#14095

Depositor

Bob Weinberg

Article

Hartwell et al Proc Natl Acad Sci U S A. 2006 Dec 12. 1

Insert

goosecoid (Gsc Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pLX302-PTP-SL-V5 puro

Plasmid

#87774

Purpose

Expresses V5 tagged human PTP-SL

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

PTP-SL (PTPRR Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

March 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 22,946 results for: Tes