We narrowed to 23,099 results for: crispr

1. pMOD6-pGRR-r11-r10-yeGFP

   Plasmid

   #74368

   Purpose

   pGRR NOR gate promoter responsive to gRNA-r11 and gRNA-r10. Integrates in the URA locus of W303 and recovers URA function.

   Depositor

   Eric Klavins

   Article

   Gander et al bioRxiv 041871

   Insert

   yeGFP
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pGRR-r11-r10

   Available Since

   April 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. p2CT-His-MBP-Ppr_Cas13a_WT

   Plasmid

   #91872

   Purpose

   Bacterial expression for Cas13a

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

   Insert

   PprCas13a (PprC2c2) WT
   

   Use

   Tags

   His6-MBP-TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pM115: CasRx control presgRNA

   Plasmid

   #166867

   Purpose

   U6-driven expression of control (non-targeting) presgRNA compatible with CasRx.

   Depositor

   Guei-Sheung Liu

   Article

   Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

   Insert

   non-targeting presgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pOC1 Gria1-GFP-FRB KI

   Plasmid

   #183428

   Purpose

   CreOFF knock-in for GluA1-GFP-FRB (amino acid position: stop codon)

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Insert

   gRNA and GFP-FRB donor
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pOC2 Dlg4-Halo-FKBP KI

   Plasmid

   #183435

   Purpose

   CreOFF knock-in for PSD95-Halo-FKBP (amino acid position: R721)

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Insert

   gRNA and Halo-FKBP donor
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCas5

   Plasmid

   #82378

   Purpose

   sgRNA targeting YFP expressed from pJ23119 in the NS1 locus with kanamycin resistance

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   gRNA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   PJ23119

   Available Since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p2CT-His-MBP-Lne_Cas13a_WT

   Plasmid

   #91866

   Purpose

   Bacterial expression for Cas13a

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

   Insert

   LneCas13a (LneC2c2) WT
   

   Use

   Tags

   His6-MBP-TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pX330A_D10A-1x6

   Plasmid

   #58776

   Purpose

   Expresses Cas9 nickase and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 (D10A) nickase
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D10A nickase-converting mutation

   Promoter

   CBh

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p2CT-His-MBP-Lbu_C2c2_R1079A_K1080A

   Plasmid

   #91861

   Purpose

   Bacterial expression for Cas13a

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

   Insert

   LbuCas13a (LbuC2c2) R0179A/K1080A
   

   Use

   Tags

   His6-MBP-TEV site

   Expression

   Bacterial

   Mutation

   K1080A

   Promoter

   Available Since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pOC3 smFP-HA-Cacna1e KI

    Plasmid

    #183436

    Purpose

    CreON FlpOFF knock-in for smFP-HA-CaV2.3 (amino acid position: G5)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and smFP HA donor
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p2CT-His-MBP-Lbu_C2c2_E299A

    Plasmid

    #91855

    Purpose

    Bacterial expression for Cas13a

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

    Insert

    LbuCas13a (LbuC2c2) E299A
    

    Use

    Tags

    His6-MBP-TEV site

    Expression

    Bacterial

    Mutation

    E299A

    Promoter

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Neg. Ctrl_sgRNA

    Plasmid

    #111298

    Purpose

    CRISPR KO murine neg. ctrl.

    Depositor

    Vamsi Mootha

    Article

    Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

    Insert

    NegCtrl sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MsCLYBL_sgRNA1

    Plasmid

    #111294

    Purpose

    CRISPR KO murine CLYBL

    Depositor

    Vamsi Mootha

    Article

    Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

    Insert

    sgRNA1 against murine CLYBL
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFETCh_ONECUT2

    Plasmid

    #135738

    Purpose

    Donor vector for 3' FLAG tag of human ONECUT2

    Depositor

    Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    ONECUT2 Homology arms (ONECUT2 Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCfB6677

    Plasmid

    #106155

    Purpose

    EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6632, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pOC1 Gria1-HA KI

    Plasmid

    #183427

    Purpose

    CreON knock-in for GluA1-HA (amino acid position: stop codon)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and HA donor
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pOC2 Dlg4-Halo KI

    Plasmid

    #183449

    Purpose

    CreOFF knock-in for PSD95-Halo (amino acid position: R721)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and Halo donor
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p2CT-His-MBP-Rca_Cas13a_WT

    Plasmid

    #91870

    Purpose

    Bacterial expression for Cas13a

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

    Insert

    RcaCas13a (RcaC2c2) WT
    

    Use

    Tags

    His6-MBP-TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSIBR002

    Plasmid

    #177665

    Purpose

    SIBR Intron 2 (Int2) interrupts the CDS of FnCas12a

    Depositor

    John van der Oost

    Article

    Patinios et al Nucleic Acids Res. 2021 Oct 6. pii: 6382

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOC2 CRISPIE-Halo-Homer1 KI

    Plasmid

    #183446

    Purpose

    CreON knock-in for Halo-Homer1 (amino acid position: G2)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and CRISPIE Halo donor
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. BPK2014-AmCas12b

    Plasmid

    #121951

    Purpose

    E. coli expression, protein purification

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    AmCas12b
    

    Use

    Tags

    10xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. MsCLYBL_sgRNA6

    Plasmid

    #111295

    Purpose

    CRISPR KO murine CLYBL

    Depositor

    Vamsi Mootha

    Article

    Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

    Insert

    sgRNA6 against murine CLYBL
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCfB6684

    Plasmid

    #106154

    Purpose

    EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6631, integration into Yarrowia lipolytica chromosomal location IntD_1, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAC-U63-tgRNA-nlsBFP

    Plasmid

    #169029

    Purpose

    gRNA-marker vector contain a U6:3 promoter, gRNA scaffold, and a Ubi-mTagBFP-NLS marker.

    Depositor

    Chun Han

    Article

    Allen et al PLoS Biol. 2021 Jan 14;19(1):e3001061. d

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6:3

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p2CT-His-MBP-Cam_Cas13a_WT

    Plasmid

    #91869

    Purpose

    Bacterial expression for Cas13a

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

    Insert

    CamCas13a (CamC2c2) WT
    

    Use

    Tags

    His6-MBP-TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p2CT-His-MBP-Ere_Cas13a_WT

    Plasmid

    #91868

    Purpose

    Bacterial expression for Cas13a

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

    Insert

    EreCas13a (EreC2c2) WT
    

    Use

    Tags

    His6-MBP-TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBZ191-pU6-sgBFP-CBh-sv40NLS-Cas9-NLS-T2A-mCherry

    Plasmid

    #170183

    Purpose

    Expression vector for a sgRNA against BFP and spCas9 linked to mCherry via a T2A peptide

    Depositor

    Hunter Fraser

    Article

    Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris

    Insert

    sgBFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCfB6682

    Plasmid

    #106152

    Purpose

    EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6627, integration into Yarrowia lipolytica chromosomal location IntC_2, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. p2CT-His-MBP-Lbu_C2c2_K1087A

    Plasmid

    #91864

    Purpose

    Bacterial expression for Cas13a

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

    Insert

    LbuCas13a (LbuC2c2) K1087A
    

    Use

    Tags

    His6-MBP-TEV site

    Expression

    Bacterial

    Mutation

    K1087A

    Promoter

    Available Since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pOC4 Actb-mRuby3 KI

    Plasmid

    #183437

    Purpose

    FlpON knock-in for mRuby3-beta-actin (amino acid position: before start codon)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and mRuby3 donor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pOC5 Tubb3-GFP KI

    Plasmid

    #183440

    Purpose

    FlpOFF knock-in for beta3-tubulin GFP (amino acid position: stop codon)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and GFP donor
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pKLV2-EF1aMS2p65HSF1hyg-W

    Plasmid

    #112920

    Purpose

    Lentiviral vector expressing MS2p65HSF1 under the control of the long human EF1a promoter

    Depositor

    Kosuke Yusa

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    MS2p65HSF1-T2Ahyg
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human EF1a promoter

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pENTR-R4R3-3xmEGFP-C

    Plasmid

    #113751

    Purpose

    Gateway multi-site entry clone for second position 3x mEGFP (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    mEGFP-mEGFP-mEGFP
    

    Use

    CRISPR; Gateway entry vector

    Tags

    3xmEGFP

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCas2F

    Plasmid

    #82377

    Purpose

    dCas9 expressed from aTc-inducible promoter in acsA, F RBS: TGGGCG

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    PEZ3

    Available Since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pX330A_D10A-1x3

    Plasmid

    #58773

    Purpose

    Expresses Cas9 nickase and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSIBR001

    Plasmid

    #177628

    Purpose

    SIBR Intron 1 (Int1) interrupts the CDS of FnCas12a

    Depositor

    John van der Oost

    Article

    Patinios et al Nucleic Acids Res. 2021 Oct 6. pii: 6382

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCas9–mKate2ps–EgRNA

    Plasmid

    #62716

    Purpose

    Empty; no U6 gRNA cassette

    Depositor

    Leonidas Bleris

    Article

    Moore et al Nucleic Acids Res. 2015 Jan 30;43(2):129

    Insert

    Cas9–mKate2ps
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

38. SIN40C.SFFV.sfGFP-RepEntry.iPAC

    Plasmid

    #100893

    Purpose

    Lentiviral CRISPR-Cas9 Reporter

    Depositor

    Dirk Heckl

    Article

    Labuhn et al Nucleic Acids Res. 2018 Feb 16;46(3):137

    Inserts

    sfGFP
    PAC
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pYPQ138A

    Plasmid

    #69280

    Purpose

    Golden Gate entry vector; 8th gRNA under AtU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pYPQ137A

    Plasmid

    #69279

    Purpose

    Golden Gate entry vector;7th gRNA under AtU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pENTR-R4R3-3xmRuby-N

    Plasmid

    #113754

    Purpose

    Gateway multi-site entry clone for second position 3x mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    mRuby2-mRuby2-mRuby2
    

    Use

    CRISPR; Gateway entry vector

    Tags

    3xmRuby2

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCfB6679

    Plasmid

    #106158

    Purpose

    EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6638, integration into Yarrowia lipolytica chromosomal location IntE_4, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pORANGE Tubb3-4xGFP KI

    Plasmid

    #183445

    Purpose

    GFP knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and 4xGFP donor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. p2CT-His-MBP-Lbu_C2c2_R1072A

    Plasmid

    #91859

    Purpose

    Bacterial expression for Cas13a

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

    Insert

    LbuCas13a (LbuC2c2) R1072A
    

    Use

    Tags

    His6-MBP-TEV site

    Expression

    Bacterial

    Mutation

    R1072A

    Promoter

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pBSV2G_PflaBS-sgRNA500

    Plasmid

    #149615

    Purpose

    gRNA expression in B. burgdorferi, parental vector

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaBS

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pBSV2G_P0026-sgRNA500

    Plasmid

    #149620

    Purpose

    gRNA expression in B. burgdorferi, parental vector

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P0026

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. BC1364-mouse U6-sgH2B

    Plasmid

    #164043

    Purpose

    U6-driven sgRNA targeting H2B

    Depositor

    Baohui Chen

    Article

    Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

    Insert

    H2B targeting sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    mouse U6

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pJZ002

    Plasmid

    #120231

    Purpose

    CRISPR-cas9 targeting in E. coli with ampicillin resistance

    Depositor

    Bradley Moore

    Article

    Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pCfB6681

    Plasmid

    #106157

    Purpose

    EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6637, integration into Yarrowia lipolytica chromosomal location IntE_3, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pX330.iGFP1

    Plasmid

    #66581

    Purpose

    sgRNA for iGFP

    Depositor

    Wen Xue

    Article

    Li et al Genome Biol. 2015 May 28;16(1):111.

    Insert

    iGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only