We narrowed to 12,028 results for: LEA

Showing: 2721 - 2740 of 12028 results

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pBabe(SV40)-Cytochrome C-GFP

Plasmid

#41184

Depositor

Douglas Green

Article

Goldstein et al Nat Cell Biol. 2000 Mar;2(3):156-62.

Insert

Cytochrome C-GFP

Use

Retroviral

Expression

Mammalian

Promoter

SV40

Available Since

Nov. 26, 2013

Availability

Academic Institutions and Nonprofits only
pEMS1052

Plasmid

#29004

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple112 (HCRT Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Cre-EGFP-NLS

Available Since

Aug. 4, 2011

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro_shDDX58_230212

Plasmid

#167293

Purpose

Lentiviral plKO.1 construct coding for a short-hairpin RNA targeting the human gene DDX58 (coding for RLR sensor RIG-I). Contains a puro resistance cassette.

Depositor

Agnel Sfeir

Article

Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

Insert

shRNA hsDDX58 (RIGI Human)

Use

Lentiviral

Expression

Mammalian

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-NLGN3 R451C

Plasmid

#59319

Purpose

Expression plasmid of HA-tagged human Neuroligin 3 harboring an R451C mutation

Depositor

Peter Scheiffele

Article

Chih et al Hum Mol Genet. 2004 Jul 15;13(14):1471-7

Insert

NLGN3 R451C (NLGN3 Human)

Tags

HA

Expression

Mammalian

Mutation

Arginine 451 to Cysteine (R451C mutation)

Promoter

CAG

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pEMS1172

Plasmid

#29301

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple151 (OLIG1 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available Since

Jan. 6, 2012

Availability

Academic Institutions and Nonprofits only

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Wild-type CoV-2 ORF8

Plasmid

#159933

Purpose

Protein overexpression in E.coli

Depositor

James Hurley

Article

Flower et al Proc Natl Acad Sci U S A. 2021 Jan 12;11

Insert

CoV-2 ORF8 18-121 (ORF8 CoV-2)

Tags

6x Histidine, TEV cleavable

Expression

Bacterial

Mutation

None

Promoter

T7

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pY006 (pcDNA3.1-hBpCpf1)

Plasmid

#69978

Purpose

Expresses humanized BpCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hBpCpf1

Use

CRISPR

Tags

NLS & 3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only
PET-HiFi SpCas9-NLS-6xHis

Plasmid

#207376

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

HiFi SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

R691A

Promoter

T7

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-DIO-GRAB_OT1.0

Plasmid

#185387

Purpose

Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent manner

Depositor

Yulong Li

Article

Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

GPCR activation based oxytocin sensor GRAB_OT1.0

Use

AAV

Promoter

EFS

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pSynapsin_NLS_GFP_P2A_Halo_GluA2

Plasmid

#170454

Purpose

Expresses halo tagged GluA2 in neuronal cells

Depositor

Shigeki Watanabe

Article

Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

Insert

GluA2 (Gria2 Mouse)

Use

Lentiviral

Tags

Halo

Expression

Mammalian

Promoter

Synapsin

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-GfaABC1D-GRAB_Ado1.0med

Plasmid

#153289

Purpose

Express the medium-affinity adenosine sensor GRAB_Ado1.0med in astrocytes

Depositor

Yulong Li

Article

Wu et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Adenosine sensor GRAB_Ado1.0med (medium affinity)

Use

AAV

Promoter

GfaABC1D

Available Since

Aug. 3, 2020

Availability

Academic Institutions and Nonprofits only

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HS-PCNA-GFP

Plasmid

#105942

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

PCNA (PCNA Human)

Expression

Mammalian

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST14-SpyCatcher002 EQ

Plasmid

#102830

Purpose

Bacterial expression of unreactive version of SpyCatcher002

Depositor

Mark Howarth

Article

Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

Insert

SpyCatcher002 EQ

Tags

GSSGS, His6, and TEV cleavage site

Expression

Bacterial

Mutation

E77Q mutation prevents isopeptide bond formation

Promoter

T7

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-CD19.28H.28TM.BB.3z

Plasmid

#200679

Purpose

Modular CD28hinge-CD28transmembrane-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

Depositor

Michele Bernasconi

Article

Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

Insert

FMC63-CD28HD-CD28TM-41BB-CD3z-P2A-EGFP (CD28, CD247, TNFRSF9 Human)

Use

Lentiviral

Tags

MycTag

Expression

Mammalian

Promoter

EF1a-short

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pLentiCMV_HyPer7RgDAAO

Plasmid

#217653

Purpose

Expresses the fusion of HyPer7,a H2O2 sensor, and Rhodotorula gracilis D amino acid oxidase (DAAO) to measure transport of D amino acids across the plasma membrane

Depositor

Seth Parker

Article

Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi

Insert

HyPer7 D amino acid oxidase

Use

Lentiviral

Tags

Nuclear export signal

Expression

Mammalian

Mutation

Fused DAAO to the C-terminus of HyPer7 using a Gl…

Promoter

CMV

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV-opEBNA2-IRES-GFP

Plasmid

#220354

Purpose

Expresses EBV latent gene EBNA2 in mammalian cells (EBNA2 coding sequence was optimised for expression and carries a C-terminal 3xHA-tag)

Depositor

Klaus Rajewsky

Article

Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

Insert

opEBNA2 (EBNA-2 Epstein-Barr Virus (EBV) strain B95-8)

Use

Retroviral

Tags

3 x HA tag

Expression

Mammalian

Mutation

codon-optimised for expression in mammalian cells

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMesh1*

Plasmid

#175591

Purpose

Lac inducible, AMP Resistant, fluor labelled (CFP) gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.

Depositor

Greg Bokinsky

Article

Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.

Insert

Mesh1 (Mesh1 Fly)

Tags

Fused with a flexible GS linker to cerulean

Expression

Bacterial

Mutation

Codon optimized for translation in E.coli. AGGAGG…

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-16-4GS-LaG-2

Plasmid

#172764

Purpose

Bacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (4GS linker)

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-16-4GS-LaG-2

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pTRIPZ shcGAS-Mmu

Plasmid

#127698

Purpose

Doxycyclin inducible shRNA knockdown of mouse cGAS gene

Depositor

Sandra Demaria

Article

Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

Insert

shcGAS Mmu (Cgas Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pNES-iRFP-C1

Plasmid

#116863

Purpose

cytosolic iRFP

Depositor

Gerry Hammond

Article

Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

Insert

iRFP713

Tags

X.leavis map2k1.L(32-44)

Expression

Mammalian

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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