We narrowed to 2,927 results for: PPL

1. pCFJ2359 - intron GG2 - 250bp

   Plasmid

   #159878

   Purpose

   Golden Gate compatible intron with PATCs for reduced germline silencing

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   intron GG2 - 250bp
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCFJ2369 - intron GG1 - 250bp

   Plasmid

   #159877

   Purpose

   Golden Gate compatible intron with PATCs for reduced germline silencing

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   intron GG1 - 250bp
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSEM253 - sgRNA mosTI hygroR

   Plasmid

   #159827

   Purpose

   Single copy or array insertion by MosTI using split hygroR selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   sgRNA mosTI hygroR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pX459_gRNA pAS single_hspCas9

   Plasmid

   #193311

   Purpose

   Cas9 from S. pyogenes and U6-pAS single sgRNA (V2.0)

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

   Insert

   Cas9 from S. pyogenes and U6-pAS single sgRNA (V2.0)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   not applicable

   Promoter

   Available since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCFJ2370 - intron GG3 - 250bp

   Plasmid

   #159879

   Purpose

   Golden Gate compatible intron with PATCs for reduced germline silencing

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   intron GG3 - 250bp
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSEM270 - MCS mosTI hygroR target

   Plasmid

   #159826

   Purpose

   Single copy or array insertion by MosTI using split hygroR selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   MCS mosTI hygroR target
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pU6-SacB-Cas9-T2A-mCherry_(BbsI)

   Plasmid

   #117070

   Purpose

   Encodes Cas9, T2A-mCherry, and AmpR which creates a CRISPR system application with reliable positive selection

   Depositor

   Kim Failor

   Inserts

   Cas9
   SacB
   

   Use

   CRISPR

   Tags

   3x FLAG, NLS, and T2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pNM3749

   Plasmid

   #154108

   Purpose

   loxP FRT FRT3 RMCE landing site in a Golden Gate SapI GCG ACG assembly vector

   Depositor

   Michael Nonet

   Article

   Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

   Inserts

   LoxP-SEC-LoxP
   FRT::rpl-28p::GFP::his-58::FRT3
   

   Use

   Cre/Lox; Rmce

   Tags

   Expression

   Worm

   Mutation

   Promoter

   not applicable

   Available since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCFJ2259 - Intron GG2 - 900 bp

   Plasmid

   #159881

   Purpose

   Golden Gate compatible intron with PATCs for reduced germline silencing

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   Intron GG2 - 900 bp
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSEM321 - array co-injection fragment mosTI hygroR

    Plasmid

    #159828

    Purpose

    Single copy or array insertion by MosTI using split hygroR selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    array co-injection fragment mosTI hygroR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCFJ1117 - [4-1] - Promoter - Psmu-1(no_atg)

    Plasmid

    #159886

    Purpose

    Three-fragment Gateway compatible promoter with PATCs for germline expression from arrays

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [4-1] - Promoter - Psmu-1(no_atg)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMDJ80 - pENTR[4-1] - Promoter - Psmu-2 (no_start)

    Plasmid

    #159887

    Purpose

    Three-fragment Gateway compatible promoter with PATCs for germline expression from arrays

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [4-1] - Promoter - Psmu-2 (no_start)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCFJ1359 - Intron GG2 - smu-1

    Plasmid

    #159805

    Purpose

    Golden Gate compatible intron with PATCs for reduced germline silencing

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    Intron GG2 - smu-1
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCFJ2215 - Intron GG3 - 900 bp

    Plasmid

    #159882

    Purpose

    Golden Gate compatible intron with PATCs for reduced germline silencing

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    Intron GG3 - 900 bp
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUDP003

    Plasmid

    #101164

    Purpose

    E. coli/S. cerevisiae amdS shuttle vector allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)

    Depositor

    Jean-Marc Daran

    Article

    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScTDH3

    Available since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRS426-TUB1-internal6xHis

    Plasmid

    #60390

    Purpose

    Expression of yeast alpha tubulin (TUB1) - internal 6xHis using a GAL promoter

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB1 (TUB1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    internal 6xHis (see supplement figure 4)

    Promoter

    GAL

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pCFJ1360 - Intron GG3 - smu-1

    Plasmid

    #159806

    Purpose

    Golden Gate compatible intron with PATCs for reduced germline silencing

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    Intron GG3 - smu-1
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCFJ1358 - Intron GG1 - smu-1

    Plasmid

    #159804

    Purpose

    Golden Gate compatible intron with PATCs for reduced germline silencing

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    Intron GG1 - smu-1
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMNK55 - piRNAi 21ur-1224 - cku-80

    Plasmid

    #159820

    Purpose

    piRNA-induced RNA interference (piRNAi) to bias double-strand repair toward homologous recombination

    Depositor

    Christian Froekjaer-Jensen

    Article

    Priyadarshini et al Nat Methods. 2022 Feb;19(2):187-194. doi

    Insert

    cku-80 piRNAi 21ur-1224
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMNK54 - piRNAi 21ur-1224 - him-5

    Plasmid

    #159818

    Purpose

    piRNA-induced RNA interference (piRNAi) to induce males in transgenic animals

    Depositor

    Christian Froekjaer-Jensen

    Article

    Priyadarshini et al Nat Methods. 2022 Feb;19(2):187-194. doi

    Insert

    him-5 piRNAi 21ur-1224
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge