We narrowed to 11,551 results for: NSI

1. pHsp70B (GB0155)

   Plasmid

   #68172

   Purpose

   Provides the Arabidopsis thaliana heat shock protein 70B promoter as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   AtHsp70B promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available Since

   Oct. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. BoxB-MS2 adRNA (RAB7A-ACG site)

   Plasmid

   #170155

   Purpose

   AAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcript for C-U editing

   Depositor

   Prashant Mali

   Article

   Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

   Insert

   BoxB-RAB7A(ACG)-MS2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available Since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAB088c

   Plasmid

   #79213

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI-TnTBR3-F3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PlacZ-opt (OR1); Ppro1

   Available Since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PXPR007 sgGFP-2

   Plasmid

   #74961

   Purpose

   Cas9 + sgGFP-2 with blasticidin selection

   Depositor

   William Hahn

   Article

   Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

   Insert

   GFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDelila (GB0079)

   Plasmid

   #68200

   Purpose

   Provides the CDS of the Antirrhinum majus Delila transcription factor as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   Delila coding region
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available Since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRosea (GB0026)

   Plasmid

   #68194

   Purpose

   Provides the CDS of the Antirrhinum majus Rosea transcription factor as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   A. majus Rosea coding region
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available Since

   Oct. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBS-I-Sce1-slc6a3[11kb]:egfp

   Plasmid

   #53240

   Purpose

   allow visualization of pretectal dopaminergic neurons in vivo from day 3 post-fertilization and allow expression of transgenes in these neurons through adulthood

   Depositor

   Edward Burton

   Article

   Bai et al Neuroscience. 2009 Dec 15;164(3):1138-51

   Insert

   DAT-GFP
   

   Use

   Cloning

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available Since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHH0103_VAV2-2/2

   Plasmid

   #91506

   Purpose

   Protein expression and purification of human SH3 domain construct VAV2-2/2

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   VAV2-2/2 (VAV2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBad-CA TAG20

   Plasmid

   #105666

   Purpose

   expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 20 and C-terminal 6x his tag

   Depositor

   Ryan Mehl

   Article

   Mehl et al 2020

   Insert

   human carbonic anhydrase II thermostable variant (CA2 Human)
   

   Use

   Tags

   6x his tag

   Expression

   Bacterial

   Mutation

   TAG stop codon at site 20

   Promoter

   pBad/arabinose

   Available Since

   March 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-nef-CIAO-TVA-mCherry

    Plasmid

    #149295

    Purpose

    Cre-dependent expression of TVA:mCherry fusion protein for rabies tracing.

    Depositor

    Edward Callaway

    Article

    Fischer et al Proc Natl Acad Sci U S A. 2019 Dec 16. p

    Insert

    TVA:mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Start codon removed.

    Promoter

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. MS2-adRNA (5'UAG)

    Plasmid

    #170126

    Purpose

    Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a UAG at the 5' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    MS2-ADAR2(5'UAG)-MS2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 and mouse U6

    Available Since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. BoxB-MS2 adRNA (RAB7A-UAG site)

    Plasmid

    #170138

    Purpose

    AAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcript

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    BoxB-RAB7A(UAG)-MS2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. BAS292 C terminal SF-GFP kanR

    Plasmid

    #74087

    Purpose

    S.pombe recoded super-folder GFP in universal C-terminal tagging plasmid, G418 resistance

    Depositor

    Bassem Al-Sady

    Article

    Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

    Insert

    SF-GFP recoded for S.pombe
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pHH0103_VAV3-1/2

    Plasmid

    #91528

    Purpose

    Protein expression and purification of human SH3 domain construct VAV3-1/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    VAV3-1/2 (VAV3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHH0103_SORBS3-1/3

    Plasmid

    #91534

    Purpose

    Protein expression and purification of human SH3 domain construct SORBS3-1/3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SORBS3-1/3 (SORBS3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHH0103_VAV3-2/2

    Plasmid

    #91524

    Purpose

    Protein expression and purification of human SH3 domain construct VAV3-2/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    VAV3-2/2 (VAV3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHH0103_SH3YL1-1/1

    Plasmid

    #91485

    Purpose

    Protein expression and purification of human SH3 domain construct SH3YL1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SH3YL1-1/1 (SH3YL1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHH0103_NEBL-1/1

    Plasmid

    #91383

    Purpose

    Protein expression and purification of human SH3 domain construct NEBL-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    NEBL-1/1 (NEBL Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHH0103_NPHP1-1/1

    Plasmid

    #91358

    Purpose

    Protein expression and purification of human SH3 domain construct NPHP1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    NPHP1-1/1 (NPHP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHH0103_PACSIN1-1/1

    Plasmid

    #91372

    Purpose

    Protein expression and purification of human SH3 domain construct PACSIN1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    PACSIN1-1/1 (PACSIN1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHH0103_SH3D21-1/3

    Plasmid

    #91296

    Purpose

    Protein expression and purification of human SH3 domain construct SH3D21-1/3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SH3D21-1/3 (SH3D21 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHH0103_MYO15A-2/2

    Plasmid

    #91291

    Purpose

    Protein expression and purification of human SH3 domain construct MYO15A-2/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    MYO15A-2/2 (MYO15A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1(+) neo with Smad5 CDS

    Plasmid

    #61800

    Purpose

    pcDNA3.1(+) with full length mouse Smad5 CDS

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    Smad5 CDS (Smad5 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

24. pExT

    Plasmid

    #36889

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    T (i.e., ATG, start codon as a first exon)
    GFP
    beta-globin intron
    Hygro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted nucleotides before nucleotide 275 and ins…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available Since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRenilla (GB0056)

    Plasmid

    #68198

    Purpose

    Provides the CDS of the Renilla protein as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Renilla coding region
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pIRES.Puro.mBeta

    Plasmid

    #24175

    Depositor

    Robert Sobol

    Article

    Jelezcova et al Mutat Res. 2010 Apr 1;686(1-2):57-67. do

    Insert

    pIRES.Puro.mBeta (Polb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    x

    Promoter

    Available Since

    April 9, 2010

    Availability

    Academic Institutions and Nonprofits only

27. pExG

    Plasmid

    #36888

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    GFP
    tdTomato-3Myc
    beta-globin intron
    Hygro
    

    Use

    Tags

    3 Myc tags

    Expression

    Mammalian

    Mutation

    deleted nucleotides after nucleotide 274, inserti…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available Since

    Aug. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJOE5751.1

    Plasmid

    #135083

    Purpose

    pBR322-based L-rhamnose-inducible vector.

    Depositor

    Josef Altenbuchner

    Article

    Hoffmann et al FEBS J. 2012 Oct;279(20):3911-24. doi: 1

    Insert

    P-rhaP-BAD-His tag-eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHES873

    Plasmid

    #87945

    Purpose

    Expresses estradiol-inducible synthetic transcription factor in yeast under control of GAL1 promoter. It activates transcription of promoters containing Zif268 binding sites.

    Depositor

    Hana El-Samad

    Article

    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

    Insert

    pGAL1- Zif268 DBD - hPR LBD - MSN2 AD (PGR Human, Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. BAS287 C terminal E2C hygBR

    Plasmid

    #74082

    Purpose

    S.pombe recoded E2Crimson in universal C-terminal tagging plasmid, hygB resistance.

    Depositor

    Bassem Al-Sady

    Article

    Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

    Insert

    E2Crimson
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

31. pHH0103_PPP1R13L-1/1

    Plasmid

    #91509

    Purpose

    Protein expression and purification of human SH3 domain construct PPP1R13L-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    PPP1R13L-1/1 (PPP1R13L Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET-30a(+)-Syk-tSH2

    Plasmid

    #111269

    Purpose

    express murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form, in E.coli strain Rosetta 2 (DE3)

    Depositor

    Carol Post

    Article

    Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

    Insert

    murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form (Syk Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. PSUC2-yEGFPCLN2PEST-pRS406

    Plasmid

    #64405

    Purpose

    SUC2 promoter driven GFP expression

    Depositor

    Claudia Vickers

    Article

    Williams et al Microb Cell Fact. 2015 Apr 1;14:43. doi:

    Insert

    SUC2 promoter (SUC2 Budding Yeast)
    

    Use

    Tags

    yEGFPCLN2PEST (destabilized yeast EGFP)

    Expression

    Yeast

    Mutation

    Promoter

    SUC2

    Available Since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET21-MRPP1_native+

    Plasmid

    #67865

    Purpose

    bacterial expression of TRMT10C, full coding sequence (mitoch. form)

    Depositor

    Walter Rossmanith

    Article

    Vilardo et al Nucleic Acids Res. 2012 Dec;40(22):11583

    Insert

    MRPP1 (TRMT10C Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mitoch. form (contains aa 40-403)

    Promoter

    T7

    Available Since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. MCP-ADAR2-DD(E488Q, N496F)

    Plasmid

    #170139

    Purpose

    AAV vector carrying the MCP-ADAR2-DD(E488Q, N496F) with a nuclear export signal

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    NES-MCP-ADAR2-DD(E488Q, N496F)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMTTH+eGFP-bromo5(N140A)

    Plasmid

    #157806

    Purpose

    Expression of synthetic protein composed of eGFP and 5 repeats of BRD4 bromodomain #1 with N140A mutation

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    eGFP-bromo5(N140A)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    N140A

    Promoter

    pTAC

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pEGB Pnos:Luciferase:Tnos (GB0112)

    Plasmid

    #68217

    Purpose

    Transcriptional unit for Firefly Luciferase Protein plant expression driven by the Nos promoter

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Luc
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos

    Available Since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLKO.1-Teton-puro-shFAK #1

    Plasmid

    #198757

    Purpose

    conditional knockdown of FAK

    Depositor

    Hyonchol Jang

    Article

    Kim et al Cancers (Basel). 2019 Sep 2;11(9). pii:

    Insert

    shFAK #1 (PTK2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLKO.1-Teton-puro-shFAK #2

    Plasmid

    #198758

    Purpose

    conditional knockdown of FAK

    Depositor

    Hyonchol Jang

    Article

    Kim et al Cancers (Basel). 2019 Sep 2;11(9). pii:

    Insert

    shFAK #2 (PTK2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. p148

    Plasmid

    #62616

    Purpose

    ePathBrick pETM6 vector encoding CsF3H, AaDFR, and DuLAR

    Depositor

    Mattheos Koffas

    Article

    Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1

    Inserts

    flavaone 3B-hydroxylase
    dihydroflavonol 4-reductase
    leucoanthocyanidin reductase
    

    Use

    Synthetic Biology

    Tags

    GBD, PDZ, and SH3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge