We narrowed to 15,088 results for: pET

1. pET21d-colEdes4/Imdes4

   Plasmid

   #107112

   Purpose

   plasmid for expression of computationally designed colicin endonuclease and immunity pair, des4

   Depositor

   Sarel Fleishman

   Article

   Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

   Insert

   colEdes4/Imdes4
   

   Tags

   His-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21d-colEdes5/Imdes5

   Plasmid

   #107113

   Purpose

   plasmid for expression of computationally designed colicin endonuclease and immunity pair, des5

   Depositor

   Sarel Fleishman

   Article

   Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

   Insert

   colEdes5/Imdes5
   

   Tags

   His-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET21d-colEdes6/Imdes6

   Plasmid

   #107114

   Purpose

   plasmid for expression of computationally designed colicin endonuclease and immunity pair, des6

   Depositor

   Sarel Fleishman

   Article

   Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

   Insert

   colEdes6/Imdes6
   

   Tags

   His-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFS_0387_pET30-RT-CRISPR_Rivularia_sp._PCC_7116_ARRAY_2

   Plasmid

   #116999

   Purpose

   Expresses RPCC7116RT-Cas1-Cas2 under pT7lac promoter, encodes RPCC7116CRISPR array 2, compatible with SENECA acquisition readout

   Depositor

   Randall Platt

   Article

   Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

   Insert

   Rivularia sp. PCC 7116 RT-Cas1-Cas2
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFS_0388_pET30-RT-CRISPR_Rivularia_sp._PCC_7116_ARRAY_2_RC

   Plasmid

   #117000

   Purpose

   Expresses RPCC7116RT-Cas1-Cas2 under pT7lac promoter, encodes RPCC7116CRISPR array 2 RC, compatible with SENECA acquisition readout

   Depositor

   Randall Platt

   Article

   Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

   Insert

   Rivularia sp. PCC 7116 RT-Cas1-Cas2
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFS_0386_pET30-RT-CRISPR_Rivularia_sp._PCC_7116_ARRAY_1_RC

   Plasmid

   #116998

   Purpose

   Expresses RPCC7116RT-Cas1-Cas2 under pT7lac promoter, encodes RPCC7116CRISPR array 1 RC, compatible with SENECA acquisition readout

   Depositor

   Randall Platt

   Article

   Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

   Insert

   Rivularia sp. PCC 7116 RT-Cas1-Cas2
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFS_0347_pET30-RT-CRISPR_Campylobacter_fetus_subsp._Fetus_ARRAY_1

   Plasmid

   #116959

   Purpose

   Expresses CfRT-Cas1-Cas2 under pT7lac promoter, encodes CfCRISPR array 1, compatible with SENECA acquisition readout

   Depositor

   Randall Platt

   Article

   Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

   Insert

   Campylobacter fetus subsp. Fetus RT-Cas1-Cas2
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFS_0348_pET30-RT-CRISPR_Campylobacter_fetus_subsp._Fetus_ARRAY_1_RC

   Plasmid

   #116960

   Purpose

   Expresses CfRT-Cas1-Cas2 under pT7lac promoter, encodes CfCRISPR array 1 RC, compatible with SENECA acquisition readout

   Depositor

   Randall Platt

   Article

   Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

   Insert

   Campylobacter fetus subsp. Fetus RT-Cas1-Cas2
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET51b(+)_MBP-nLucCP-65/68

   Plasmid

   #113921

   Purpose

   Plasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 65 and 68

   Depositor

   Kai Johnsson

   Article

   Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

   Insert

   MBP-NanoLucCP-65/68
   

   Use

   Luciferase

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET51b(+)_MBP-nLucCP-65/66

    Plasmid

    #113920

    Purpose

    Plasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 65 and 66

    Depositor

    Kai Johnsson

    Article

    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

    Insert

    MBP-NanoLucCP-65/66
    

    Use

    Luciferase

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET51b(+)-LUCIDRS-MTX

    Plasmid

    #113925

    Purpose

    Plasmid encoding the bacterial expression of the red-shifted LUCID semi-synthetic BRET biosensor for methotrexate

    Depositor

    Kai Johnsson

    Article

    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

    Insert

    LUCID_RS-MTX
    

    Use

    Luciferase

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET29b-clbI S178A

    Plasmid

    #114159

    Purpose

    Expresses ClbI S178A

    Depositor

    Emily Balskus

    Article

    Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

    Insert

    clbI
    

    Tags

    6xHis and 6xHis-Thrombin site

    Expression

    Bacterial

    Mutation

    Mutated S178 to Ala

    Available Since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a-clbH-C-A2-PCP

    Plasmid

    #114157

    Purpose

    Expresses ClbH-C-A2-PCP

    Depositor

    Emily Balskus

    Article

    Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

    Insert

    clbH
    

    Tags

    6xHis-thrombin-T7 tag

    Expression

    Bacterial

    Mutation

    Only contains the C-A2-PCP domains of ClbH

    Available Since

    Aug. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a_6HT-TBP6.7(P46A)

    Plasmid

    #112721

    Purpose

    This mutation (P46A) marks the first residue within the evolved region of U1A.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(P46A)
    

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    P46A

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a_6HT-TBP6.7(R47A)

    Plasmid

    #112722

    Purpose

    Arg47 within TBP6.7 is the most critical residue in terms of forming interactions with WT TAR. Mutating this amino acid to Ala results in ~600-fold reduction in Kd.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(R47A)
    

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    R47A

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a_6HT-TBP6.7(R47K)

    Plasmid

    #112723

    Purpose

    Arg47 of TBP6.7 interacts with Hoogsteen edge of TAR's Gua26. Mutating this amino acid to Lys reduces binding to the WT TAR RNA ~330-fold.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(R47K)
    

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    R47K

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a_6HT-TBP6.7(Q48A)

    Plasmid

    #112724

    Purpose

    Gln48 contacts the backbone of TAR RNA as well as mediates intramoecular interaction to stabilize the conformation of beta2-beta3 loop within TBP6.7.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(Q48A)
    

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    Q48A

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a_6HT-TBP6.7(Q48T)

    Plasmid

    #112725

    Purpose

    This mutant of TBP6.7 binds to TAR very similarly to TBP6.7(WT), with only 1.4-fold reduced Kd.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(Q48T)
    

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    Q48T

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a_6HT-TBP6.7(T50A)

    Plasmid

    #112727

    Purpose

    Lav-evolved amino acid T50 within TBPs is important in engaging intramolecularly to steer also lab-evolved R49 into conformation compatible binding to RNA.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(T50A)
    

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    T50A

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a_6HT-TBP6.7(P51A)

    Plasmid

    #112728

    Purpose

    Similar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(P51A)
    

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    P51A

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge