We narrowed to 13,107 results for: CRISPR-Cas9

1. FUGW U6 gLacZ dCas9-KRAB-T2a-GFP

   Plasmid

   #234883

   Purpose

   non-targeting CRISPRi control

   Depositor

   Johan Jakobsson

   Article

   Adami et al bioRxiv 2025.01.17.633315

   Insert

   L1HS gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CMV-Hsp1-Hsp2Cas9-KY-P2A-puro

   Plasmid

   #192133

   Purpose

   Expresses highly specific Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

   Depositor

   Yongming Wang

   Article

   Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

   Insert

   Hsp1-Hsp2Cas9-KY
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Hsp1-Hsp2Cas9 (K390A, Y446A)

   Promoter

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. IF405: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-p300core

   Plasmid

   #121825

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the p300 catalytic core for 3 or 4-component MultiSite Gateway Pro assembly.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   Sa-dCas9/p300core (open)
   

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   NLS

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. IA304: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-VPR

   Plasmid

   #121822

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the VPR transcriptional activator for 3 or 4-component MultiSite Gateway Pro assembly.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   Sa-dCas9/VPR (open)
   

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   NLS

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. LLP774_dCas9-Spy-Snoop-Sunx5-Avi-Tag-BFP (dCas9-SSSavi-BFP)

   Plasmid

   #211767

   Purpose

   dCas9 docking array with four tag domains (Spy, Snoop, aGCN4, Avi), with BFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   dCas9, Spy, Snoop, aGCN4, AviTags
   

   Use

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   dCas9 D10A and H840A

   Promoter

   pGK and pEF1a

   Available since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR-pSFFV-dCas9-SunTag-P2A-BFP-dWPRE

   Plasmid

   #122151

   Purpose

   Expresses dCas9-SunTag(24X) fusion and a fluorescent indicator BFP.

   Depositor

   Clifford Brangwynne

   Article

   Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

   Insert

   dCas9 fused to SunTag(24x)
   

   Use

   Lentiviral

   Tags

   2xNLS-P2A-BFP and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   March 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMEH305 HA NLS SpCas9 3x high affinity MS2 loops

   Plasmid

   #168216

   Purpose

   Mammalian expression SV40 and nucleoplasmin NLS SpCas9 with 3 high affinity MS2 loops and fused to an N-terminal HA tag

   Depositor

   Joshua Leonard

   Article

   Hung et al J Extracell Vesicles. 2016 May 13;5:3102

   Insert

   SV40 NLS SpCas9
   

   Use

   Lentiviral

   Tags

   HA, SV40 NLS, and nucleoplasmin NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-Zif268

   Plasmid

   #69224

   Purpose

   Expresses R1335K mutant SpCas9 fused to Zif268 in mammalian cell

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   NLS-3XHA-NLS and Zif268

   Expression

   Mammalian

   Mutation

   Arginine 1335 is changed to Lysine

   Promoter

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-ZFP_TS2

   Plasmid

   #69228

   Purpose

   Expresses R1335K mutant SpCas9 fused to ZFP-TS2 in mammalian cell

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   TS2 ZFP
   

   Use

   CRISPR

   Tags

   NLS-3XHA-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-ZFP_TS4

    Plasmid

    #69230

    Purpose

    Expresses R1335K mutant SpCas9 fused to ZFP-TS4 in mammalian cell

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    TS4 ZFP
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-TALETS3

    Plasmid

    #107301

    Purpose

    Expresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and TALE-VEGFA-TS3 in mammalian cells

    Depositor

    Scot Wolfe

    Insert

    Truncated SpCas9 (aa 574-1368) fused to FKBP and TALE
    

    Use

    CRISPR

    Tags

    FKBP, SV40NLS, 3xHA, c-Myc NLS, and TALE_VEGFA-TS…

    Expression

    Mammalian

    Mutation

    Truncated SpCas9 (aa 574-1368) with R1335K mutati…

    Promoter

    CMV IE94

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCC_01 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9-NLS-2A-Puro-WPRE

    Plasmid

    #139086

    Purpose

    Expresses human codon-optimized SpCas9 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    SpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCC_02 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9NG-NLS-2A-Puro-WPRE

    Plasmid

    #139087

    Purpose

    Expresses human codon-optimized Cas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    SpCas9-NG
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    L1111R, D1135V,G1218R, E1219F, A1322R, R1335V, T1…

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCC_03 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9-NLS-2A-Puro-WPRE

    Plasmid

    #139088

    Purpose

    Expresses human codon-optimized xCas9 3.7 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    xCas9 3.7
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    A262T, R324L,S409I, E480K, E543D, M694I, E1219V

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. lentiCRISPR - EGFP sgRNA 1

    Plasmid

    #51760

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

    Inserts

    Cas9
    Puromycin resistance
    EGFP sgRNA 1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and U6

    Available since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. lentiCRISPR - EGFP sgRNA 2

    Plasmid

    #51761

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

    Inserts

    Cas9
    Puromycin resistance
    EGFP sgRNA 2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and U6

    Available since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. lentiCRISPR - EGFP sgRNA 4

    Plasmid

    #51763

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

    Inserts

    Cas9
    Puromycin resistance
    EGFP sgRNA 4
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and U6

    Available since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. lentiCRISPR - EGFP sgRNA 5

    Plasmid

    #51764

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

    Inserts

    Cas9
    Puromycin resistance
    EGFP sgRNA 5
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS and U6

    Available since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. lentiCRISPR - EGFP sgRNA 3

    Plasmid

    #51762

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

    Inserts

    Cas9
    Puromycin resistance
    EGFP sgRNA 3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and U6

    Available since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. lentiCRISPR - EGFP sgRNA 6

    Plasmid

    #51765

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

    Inserts

    Cas9
    Puromycin resistance
    EGFP sgRNA 6
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and U6

    Available since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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