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Plasmid#67434PurposeMammalian expression of hArf4 with C-term V5 and His6 tagDepositorInsertARF4 (ARF4 Human)
UseTagsHis and V5ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-ARL4-GFP
Plasmid#67398PurposeMammalian expression of hArl4 with C-term GFP tagDepositorInsertARL4 (ARL4A Human)
UseTagsGFPExpressionMammalianMutationbp 105 A to T; silent mutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-ARF1-V5-His6
Plasmid#67432PurposeMammalian expression of hArf1 with C-term V5 and His6 tagDepositorInsertARF1 (ARF1 Human)
UseTagsHis and V5ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-ARFRP1
Plasmid#67471PurposeMammalian expression of hArfrp with stop codon (native expression)DepositorInsertARFRP1 (ARFRP1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G8_MTOR_Nick_Exon-53_Dual_sgRNA
Plasmid#178107PurposeControl vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G8 and MTOR Nick exon-53 sgRNAs from two independent U6 promoters.DepositorInsertATP1A1 G8 + MTOR nick exon-53 sgRNAs
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-ARF4-GFP
Plasmid#67392PurposeMammalian expression of hArf4 with C-term GFP tagDepositorInsertARF4 (ARF4 Human)
UseTagsGFPExpressionMammalianMutationV53A, L123P and M134I mutations were unintended P…PromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c2X-ORF24-NTD
Plasmid#138465PurposeExpresses MBP-tagged ORF24-NTD in bacteriaDepositorInsertORF24 (ORF24 KSHV (HHV-8))
UseTagsMBPExpressionBacterialMutationAmino acids 1-201PromotertacAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1-ORF24-NTD
Plasmid#138464PurposeExpresses GST-tagged ORF24-NTD in bacteriaDepositorInsertORF24 (ORF24 KSHV (HHV-8))
UseTagsGSTExpressionBacterialMutationAmino acids 1-201PromotertacAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G6_T804N_MTOR_E2419K_Dual_pegRNA
Plasmid#178110PurposeControl vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 T804N-G6 and MTOR-E2419K pegRNAs from two independent U6 promoters.DepositorInsertATP1A1 G6 T804N pegRNA + MTOR-E2419K pegRNA
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF24-3xFLAG
Plasmid#138423PurposeExpresses full-length ORF24 with a C-terminal 3xFLAG tag in mammalian cellsDepositorInsertORF24 (ORF24 KSHV (HHV-8))
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Neuroserpin
Plasmid#58259PurposeRetroviral vector for expressing Neuroserpin in mammalian cellsDepositorInsertNeuroserpin (SERPINI1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARL1
Plasmid#67316PurposehArl1 with stop codon in Gateway Entry vectorDepositorInsertARL1 (ARL1 Human)
UseGateway entry vectorTagsExpressionBacterialMutationbp 231 C to T; silent mutationPromoterNoneAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G3_RUNX1_Nick-38_Dual_sgRNA
Plasmid#173214PurposeControl vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and RUNX1 nick-38 sgRNAs from two independent U6 promoters.DepositorInsertATP1A1 G3 sgRNA + RUNX1 Nick-38 sgRNA
UseCRISPR; Prime editing (pe3)TagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARFRP1-no stop
Plasmid#67343PurposehArfrp1 without stop codon in Gateway Entry vectorDepositorInsertARFRP1 (ARFRP1 Human)
UseGateway entry vectorTagsExpressionBacterialMutationPromoterNoneAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDSHA-ARL5A-Myc
Plasmid#67357PurposeMammalian expression of hArl5 with C-term Myc tagDepositorInsertARL5A (ARL5A Human)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV3-SMAC-HA-eGFP
Plasmid#67489PurposeTargets HA-eGFP to mitochondrial intermembrane space (IMS)DepositorInsertSMAC (DIABLO Human)
UseTagsHA-eGFPExpressionMammalianMutationaa1-59Promoterattenuated CMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
p6H-SUMO3-ORF24-NTD-Strep
Plasmid#138467PurposeExpresses SUMO3-tagged ORF24-NTD-Strep in bacteriaDepositorInsertORF24 (ORF24 KSHV (HHV-8))
UseTagsSUMO3 and Strep-Tag IIExpressionBacterialMutationAmino acids 2-201PromoterT7Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQLink-GST-4xCTD
Plasmid#138471PurposeExpresses GST-tagged 4xCTD heptapeptide repeat in bacteriaDepositorInsertRpb1 (POLR2A Human)
UseTagsGSTExpressionBacterialMutation4x CTD repeatPromoterT5Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-ARL8A-V5-His6
Plasmid#67445PurposeMammalian expression of hArl8 with C-term V5 and His6 tagDepositorInsertARL8A (ARL8A Human)
UseTagsHis and V5ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-ARL1
Plasmid#67458PurposeMammalian expression of hArl1 with stop codon (native expression)DepositorInsertARL1 (ARL1 Human)
UseTagsExpressionMammalianMutationbp 231 C to T; silent mutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-ARF3-V5-His6
Plasmid#67433PurposeMammalian expression of hArf3 with C-term V5 and His6 tagDepositorInsertARF3 (ARF3 Human)
UseTagsHis and V5ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-HA-Flag-natT1R2 ECD
Plasmid#113954Purposemammalian expression plasmid for FLAG-tagged human T1R2 extracellular domain with signal peptide of influenza hemagglutininDepositorInsertT1R2 (TAS1R2 Human)
UseTagsFLAG and Signal/leader sequence from influenza he…ExpressionMammalianMutationresidues 22-568PromotercmvAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAd388-shLuc
Plasmid#83275PurposeshRNA targeting luciferaceDepositorInsertshRNA to Luciferase
UseRetroviralTagsExpressionMutationPromoterpUC oriAvailable sinceDec. 2, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDEST40-ARL8B-V5-His6
Plasmid#67446PurposeMammalian expression of hArl8b with C-term V5 and His6 tagDepositorInsertARL8B (ARL8B Human)
UseTagsHis and V5ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV2-SMAC-HA-eGFP
Plasmid#67488PurposeTargets HA-eGFP to mitochondrial intermembrane space (IMS)DepositorInsertSMAC (DIABLO Human)
UseTagsHA-eGFPExpressionMammalianMutationaa1-59Promoterattenuated CMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-SMAC-HA-eGFP
Plasmid#67491PurposeTargets HA-eGFP to mitochondrial intermembrane space (IMS)DepositorInsertSMAC (DIABLO Human)
UseTagsHA-eGFPExpressionMammalianMutationaa1-59Promoterattenuated CMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDSHA-ARL6-Myc
Plasmid#67359PurposeMammalian expression of hArl6 with C-term Myc tagDepositorInsertARL6 (ARL6 Human)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVenus-N1 cyclin B1 L45A (1324)
Plasmid#61851Purposemammalian expression of cyclin B1 L45 A mutant fused to VenusDepositorInsertcyclin B1 (CCNB1 Human)
UseTagsVenusExpressionMammalianMutationL45APromoterCMVAvailable sinceFeb. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-ARL2-V5-His6
Plasmid#67438PurposeMammalian expression of hArl2 with C-term V5 and His6 tagDepositorInsertARL2 (ARL2 Human)
UseTagsHis and V5ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
kank3_pJet1.2
Plasmid#121981Purposefor generating kank3 riboprobes for WISH in zebrafishDepositorInsertkank3 (kank3 Zebrafish)
UseCloning vectorTagsExpressionMutationPromoterT7Available sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARL6-V5-His6
Plasmid#67422PurposeExpression of hArl6 with C-term V5 and His6 tag in E. coliDepositorInsertARL6 (ARL6 Human)
UseTagsHis and V5ExpressionBacterialMutationPromoterT7/lacOAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARL8B-no stop
Plasmid#67335PurposehArl8b without stop codon in Gateway Entry vectorDepositorInsertARL8B (ARL8B Human)
UseGateway entry vectorTagsExpressionBacterialMutationPromoterNoneAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARL6-no stop
Plasmid#67329PurposehArl6 without stop codon in Gateway Entry vectorDepositorInsertARL6 (ARL6 Human)
UseGateway entry vectorTagsExpressionBacterialMutationPromoterNoneAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARL11-no stop
Plasmid#67341PurposehArl11 without stop codon in Gateway Entry vectorDepositorInsertARL11 (ARL11 Human)
UseGateway entry vectorTagsExpressionBacterialMutationbp 442 T to C; aa C148RPromoterNoneAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-ARL6-V5-His6
Plasmid#67443PurposeMammalian expression of hArl6 with C-term V5 and His6 tagDepositorInsertARL6 (ARL6 Human)
UseTagsHis and V5ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARL1-no stop
Plasmid#67317PurposehArl1 without stop codon in Gateway Entry vectorDepositorInsertARL1 (ARL1 Human)
UseGateway entry vectorTagsExpressionBacterialMutationbp 231 C to T; silent mutationPromoterNoneAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-eBE-S1
Plasmid#101092PurposeExpresses eBE-S1 in mammalian cellsDepositorInserteBE-S1
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARL4C-V5-His6
Plasmid#67423PurposeExpression of hArl4c with C-term V5 and His6 tag in E. coliDepositorInsertARL7 (ARL4C Human)
UseTagsHis and V5ExpressionBacterialMutationPromoterT7/lacOAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARL11
Plasmid#67340PurposehArl11 with stop codon in Gateway Entry vectorDepositorInsertARL11 (ARL11 Human)
UseGateway entry vectorTagsExpressionBacterialMutationbp 261 G to A; silent mutation; bp 442 T to C; aa…PromoterNoneAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pWzyE-His
Plasmid#196669PurposeExpression of C-terminally His10-tagged WzyE from Pectobacterium atrosepticum in E.coliDepositorInsertWzyE
UseTagsTEV cleavable His10ExpressionBacterialMutationPromoteraraBADAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_T804N_hPGK1_mScarlet-I-TOSI_Donor
Plasmid#173208PurposeHDR donor plasmid to introduce the T804N mutation conferring cellular resistance to ouabain and the mScarlet-I mTOR signaling indicator (TOSI) cassette to ATP1A1 intron 17.DepositorInsertATP1A1-T804N mScarlet-I-TOSI donor
UseCRISPRTagsExpressionMammalianMutationPromoterhPGK1Available sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-MAFG-V5-miniTurbo
Plasmid#207818PurposeC-terminal tagged MAFG for miniTurbo proximity experimentsDepositorInsertMAFG-V5-miniTurbo
UseLentiviralTagsV5-miniTurboExpressionMutationPromoterUBCAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-MAFG
Plasmid#207817PurposeN-terminal tagged MAFG for miniTurbo proximity experimentsDepositorInsertminiTurbo-V5-MAFG
UseLentiviralTagsminiTurbo-V5ExpressionMutationPromoterUBCAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Cas9-NS-A
Plasmid#207823PurposeDual expression of Cas9 and non-specific sgRNADepositorInsertnon-specific sgRNA
UseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterEF-1a; U6Available sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Cas9-NS-B
Plasmid#207824PurposeDual expression of Cas9 and non-specific sgRNADepositorInsertnon-specific sgRNA
UseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterEF-1a; U6Available sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-ZNF746-C571A
Plasmid#207826PurposeDox-inducible expression of ZNF746-C571ADepositorInsertZNF746-C571A (ZNF746 Human)
UseLentiviralTagsExpressionMutationC571APromoterTREAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-zeo G226A E321K ORF29
Plasmid#200026PurposeLentiviral vector for expression of KSHV ORF29 with G226A and E321K mutationDepositorInsertORF29
UseLentiviralTagsExpressionMutationGlycine 226 changed to alanine, glutamic acid 321…PromoterCMVAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pminiT-mEmerald-MED1 donor
Plasmid#197867PurposeDonor plasmid used for building mEmerald-MED1 knock-in mESCsDepositorInsertCRSP210 (Med1 Mouse)
UseMouse TargetingTagsmEmeraldExpressionMutationPromoterNAAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTij_sg_mmu_Med6_C_terminal
Plasmid#197869PurposeCRISPR/Cas9/sgRNA plasmid for cutting Med6 C terminal and building MED6-mEmerald/Halo knock-in mESCsDepositorInsertMed6 (Med6 Mouse)
UseTagsExpressionMammalianMutationPromoterU6Available sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G3_EBFP_Nick_Dual_sgRNA
Plasmid#178094PurposeControl vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and EBFP Nick sgRNAs from two independent U6 promoters.DepositorInsertATP1A1 G3 + EBFP nick sgRNAs
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only