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pFast-FT-N1

Plasmid

#31910

Depositor

Vladislav Verkhusha

Publication

Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

Insert

Fast-FT

Expression

Mammalian

Mutation

M18V/E34K/K69R/L84W/S149T/A224S compared to mCher…

Promoter

CMV

Available since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLPCX mito Grx1-roGFP2

Plasmid

#64977

Purpose

Mammalian expression of mitochondrial Grx1-roGFP2 (retroviral vector)

Depositor

Tobias Dick

Publication

Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

Insert

Glutaredoxin-1 (GLRX Human)

Use

Retroviral

Tags

roGFP2

Expression

Mammalian

Mutation

mitochondrial targeting sequence

Available since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pMedium-FT-N1

Plasmid

#31911

Depositor

Vladislav Verkhusha

Publication

Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

Insert

Medium-FT

Expression

Mammalian

Mutation

M18L/N23D/T43S/K69R/L84W/M152I/Q194L/L205M/Y221C/…

Promoter

CMV

Available since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMC_mNG2(11)_BSDminus_F2

Plasmid

#184164

Purpose

Donor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.

Depositor

Ophir Shalem

Publication

Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

Inserts

mNG2(11)
bsd

Use

CRISPR

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pMC_mNG2(11)_BSDminus_F0

Plasmid

#184162

Purpose

Donor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 0, as well as a blasticidin resistance gene for selection of genomic integrants.

Depositor

Ophir Shalem

Publication

Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

Inserts

mNG2(11)
bsd

Use

CRISPR

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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Dominant negative Hsp90 beta

Plasmid

#22480

Depositor

William Sessa

Publication

Miao et al Arterioscler Thromb Vasc Biol. 2008 Jan

Insert

Dominant negative Hsp90 (HSP90AB1 Human)

Tags

HA

Expression

Mammalian

Available since

Dec. 8, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pSP64T X11 (DM#103)

Plasmid

#15043

Depositor

Douglas Melton

Publication

Ku et al Development. 1993 Dec . 119(4):1161-73.

Insert

Xwnt-11 (wnt11b.L Frog)

Expression

Bacterial

Available since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
pEGFP_sfCherry2(11)_Keratin

Plasmid

#82604

Purpose

Expresses sfCherry2(11) tagged keratin in mammalian cells

Depositor

Bo Huang

Publication

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

sfCherry2(11)_Keratin

Expression

Mammalian

Mutation

changed Gly12 to Ala in sfCherry11+

Available since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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pSlow-FT-N1

Plasmid

#31912

Depositor

Vladislav Verkhusha

Publication

Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

Insert

Slow-FT

Expression

Mammalian

Mutation

M18V/E30V/K69R/L84W/A179V compared to mCherry (bu…

Promoter

CMV

Available since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pGTR

Plasmid

#63143

Purpose

PCR template to construct polycistronic tRNA-gRNA

Depositor

Yinong Yang

Publication

Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

Insert

gRNA-tRNA fusion

Use

CRISPR and Synthetic Biology

Available since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pcDNA cyclin D1 HA T286A

Plasmid

#11182

Depositor

Bruce Zetter

Publication

Newman et al J Biol Chem. 2004 Oct 1. 279(40):41504-1

Insert

cyclin D1 T286A (CCND1 Human)

Tags

HA

Expression

Mammalian

Mutation

T286A. Mutant that cannot be degraded via the u…

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pLPCX cyto Grx1-roGFP2

Plasmid

#64975

Purpose

Mammalian expression of cytosolic Grx1-roGFP2 (retroviral vector)

Depositor

Tobias Dick

Publication

Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

Insert

Glutaredoxin-1 (GLRX Human)

Use

Retroviral

Tags

roGFP2

Expression

Mammalian

Available since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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NH-11

Plasmid

#87516

Purpose

For library amplification of fragment NH-11

Depositor

Steve Pollard

Publication

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Insert

NH-11

Use

Synthetic Biology and TALEN; Tale dbd library pre…

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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NG-11

Plasmid

#87500

Purpose

For library amplification of fragment NG-11

Depositor

Steve Pollard

Publication

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Insert

NG-11

Use

Synthetic Biology and TALEN; Tale dbd library pre…

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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NI-11

Plasmid

#87484

Purpose

For library amplification of fragment NI-11

Depositor

Steve Pollard

Publication

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Insert

NI-11

Use

Synthetic Biology and TALEN; Tale dbd library pre…

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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HD-11

Plasmid

#87468

Purpose

For library amplification of fragment HD-11

Depositor

Steve Pollard

Publication

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Insert

HD-11

Use

Synthetic Biology and TALEN; Tale dbd library pre…

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pEIGW Grx1-roGFP2

Plasmid

#64990

Purpose

Mammalian expression of cytosolic Grx1-roGFP2 (lentiviral vector)

Depositor

Tobias Dick

Publication

Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

Insert

Glutaredoxin-1 (GLRX Human)

Use

Lentiviral

Tags

roGFP2

Expression

Mammalian

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pcDNA cyclin D1 HA

Plasmid

#11181

Depositor

Bruce Zetter

Publication

Newman et al J Biol Chem. 2004 Oct 1. 279(40):41504-1

Insert

cyclin D1 (CCND1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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1203W-CMV-Cas7.1-7.2-bGHpolyA

Plasmid

#220961

Purpose

Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and Cas7.2 domains from DiCas7-11, deactivated (truncated from DiCas7-11 effector)

Depositor

Omar Akbari

Insert

Truncated DiCas7-11

Use

CRISPR

Expression

Mammalian

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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NYESO beta/alpha into TRAC HDRT Source (pTR 169)

Plasmid

#112021

Purpose

DNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-alpha

Depositor

Alexander Marson

Publication

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

NYESO beta/alpha into TRAC HDRT

Use

CRISPR and Synthetic Biology

Available since

Sept. 1, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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