Addgene

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LYN11-FRB-CFP

Plasmid

#38003

Depositor

Robin Irvine

Article

Hammond et al Science. 2012 Jun 21.

Inserts

LYN (LYN Human)
MTOR (MTOR )

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2012

Availability

Academic Institutions and Nonprofits only
pARF1(T31N)-CFP

Plasmid

#11384

Depositor

Joel Swanson

Article

Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

Insert

ADP Ribosylation Factor 1 (ARF1 Human)

Use

Tags

CFP

Expression

Mammalian

Mutation

Changed Threonine 31 to Asparagine.

Promoter

Available since

March 3, 2006

Availability

Academic Institutions and Nonprofits only

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pMRX-IP/SECFP-hATG9A

Plasmid

#58995

Purpose

Expresses SECFP-hATG9A in mammalian cells

Depositor

Noboru Mizushima

Article

Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

Insert

Homo sapiens autophagy related 9A (PPARG Human)

Use

Retroviral

Tags

Venus

Expression

Mutation

Promoter

Available since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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289 RFP/CFP

Plasmid

#83321

Purpose

fluorescent signal joint substrate

Depositor

Katheryn Meek

Article

Neal et al J Immunol. 2016 Apr 1;196(7):3032-42. do

Insert

RFP +CFP joining substrate

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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isce rfp/cfp

Plasmid

#83323

Purpose

fluorescent Isce joint substrate

Depositor

Katheryn Meek

Article

Neal et al J Immunol. 2016 Apr 1;196(7):3032-42. do

Insert

RFP +CFP joining substrate

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pARF6(T27N)-CFP

Plasmid

#11386

Depositor

Joel Swanson

Article

Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

Insert

ADP Ribosylation Factor 6 (ARF6 Human)

Use

Tags

CFP

Expression

Mammalian

Mutation

Changed Threonine 27 to Asparagine.

Promoter

Available since

March 3, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRX-IP/SECFP-hATG16A1

Plasmid

#58994

Purpose

Expresses SECFP-hATG16A1 in mammalian cells

Depositor

Noboru Mizushima

Article

Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

Insert

Homo sapiens autophagy related 16-like 1 (S. cerevisiae) (ATG16L1 Human)

Use

Retroviral

Tags

SECFP

Expression

Mutation

Promoter

Available since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pARF6(Q67L)-CFP

Plasmid

#11387

Depositor

Joel Swanson

Article

Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

Insert

ADP Ribosylation Factor 6 (ARF6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pQC-FsCFP-mCherryFP

Plasmid

#154836

Purpose

Frame-shift fluorescent reporter for genomic DNA editing

Depositor

Fangliang Zhang

Article

Kumar et al BMC Genomics. 2019 Jul 24;20(1):609. doi

Insert

targeting sequence of VEGF

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Vimentin - APEX2 in pECFP

Plasmid

#66170

Purpose

APEX2 fused to the C-terminus human vimentin

Depositor

Alice Ting

Article

Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

Insert

human Vimentin - APEX2 (VIM Human, Synthetic)

Use

Tags

Flag epitope tag (DYKDDDDK)

Expression

Mammalian

Mutation

APEX2 contains 4 mutations relative to wt soybean…

Promoter

CMV

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TRAM-CFP

Plasmid

#13027

Depositor

Doug Golenbock

Insert

Toll-like Receptor Adaptor Molecule (TICAM2 Human)

Use

Tags

CFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 3, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pCrysβ:ECFP-LexOP:mCherry-NRasQ61K

Plasmid

#89886

Purpose

Human NRasQ61K fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

Depositor

Tatjana Sauka-Spengler

Article

Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

Insert

mCherry-NRasQ61K

Use

Unspecified

Tags

mCherry

Expression

Mutation

Promoter

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
cyto-CFP-FRBx5

Plasmid

#103776

Purpose

expresses iPOLYMER component in mammalian cells

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

cyto-CFP-FRBx5

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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miniTn7(Gm)PA1/04/03-ecfp-a

Plasmid

#111619

Purpose

eCFP Fluorescent tagging of bacteria at their Tn7-site

Depositor

Tim Tolker-Nielsen

Article

Klausen et al Mol Microbiol. 2003 Jun;48(6):1511-24.

Insert

PA1/04/03-ecfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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NPC1 His6 ECFP

Plasmid

#53522

Depositor

Matthew Scott

Article

Lopez et al J Neurosci. 2011 Mar 23;31(12):4367-78.

Insert

NPC1

Use

Tags

ECFP and His6

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
Flag-ECFP-betaPIXa

Plasmid

#15235

Depositor

Rick Horwitz

Article

Mayhew et al J Proteome Res. 2006 Sep . 5(9):2417-23.

Insert

beta-PIXa (Arhgef7 Mouse)

Use

Tags

ECFP and Flag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only
pCrysβ:ECFP-LexOP:mCherry-HrasG12V

Plasmid

#89887

Purpose

Human HRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

Depositor

Tatjana Sauka-Spengler

Article

Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

Insert

mCherry-HRasG12V

Use

Unspecified

Tags

mCherry

Expression

Mutation

Promoter

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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PIP2-CFP(ST) sensor

Plasmid

#170355

Purpose

CFP(ST)-PH fusion used to measure realtime changes in PIP2 levels.

Depositor

Kees Jalink

Article

van der Wal et al J Biol Chem. 2001 May 4. 276(18):15337-4

Insert

CFP(ST)-PH (PLCD1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
ECFP-TevS-YPET

Plasmid

#100097

Purpose

FRET-based ECFP-YPET reporter that is cleaved by TEV

Depositor

Jim Wells

Article

Gray et al Cell. 2010 Aug 20;142(4):637-46. doi: 10

Insert

ECFP-TevS-YPET

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-TLR6-CFP

Plasmid

#13021

Depositor

Doug Golenbock

Insert

Toll-Like Receptor 6 (TLR6 Human)

Use

Tags

CFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 3, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge