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pAAV-ND4 Right-GSVG-UGI

Plasmid

#187414

Purpose

human mitochondrial DNA ND4 target TALE

Depositor

Jin-Soo Kim

Article

Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

Insert

DddA GSVG variant

Use

AAV and TALEN

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-ND4 Right-E1347A-UGI

Plasmid

#187415

Purpose

human mitochondrial DNA ND4 target TALE

Depositor

Jin-Soo Kim

Article

Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

Insert

DddA GSVG variant

Use

AAV and TALEN

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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ND4 right TALE–G1333 DddAtox N

Plasmid

#158096

Purpose

expresses the right-side ND4 TALE–DddAtox half in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

COX8A MTS–3xFLAG–ND4 right TALE–G1333 DddA tox-N–1xUGI

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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p3s-ND5-Nonsense-Right-G1333-C

Plasmid

#169215

Purpose

Express DdCBE for ND5 Nonsense Mutation

Depositor

Jin-Soo Kim

Article

Lee et al Nat Commun. 2021 Feb 19;12(1):1190. doi:

Insert

DdCBE for ND5 mutation

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pTre-Tight-luciferase-Hottip (522)

Plasmid

#65490

Purpose

positive control for lncRNA expression assay

Depositor

Rory Johnson, Roderic Guigo

Insert

Hottip (HOTTIP Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

tight TRE promoter

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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KA2880_pPB-TREtight-FLAG-Esrrb-InO

Plasmid

#124178

Purpose

piggyBac-based Dox-inducible expression of FLAG-Esrrb

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

FLAG-Esrrb-IRES-H2B-mKO2 (Esrrb Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

tetO-CMVmin

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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KA2897_pPB-TREtight-UBCmin-cHA-IRESH2BmKO2

Plasmid

#124176

Purpose

piggyBac-based Dox-inducible expression (moderate)

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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KA2944_pPB-TREtight-Esrrb-FLAG-InO

Plasmid

#124179

Purpose

piggyBac-based Dox-inducible expression of Esrrb-FLAG

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Esrrb-FLAG-IRES-H2B-mKO2 (Esrrb Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

tetO-CMVmin

Available since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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A24M04 Ig kappa light chain-pFEEKW

Plasmid

#234810

Purpose

Express Ig kappa light chain containing the VL region of the monoclonal antibody, A24M04, specific for human papilloma virus16 L1

Depositor

Susan Pierce

Article

Torgbor et al Proc Natl Acad Sci U S A. 2025 Mar 11;12

Insert

A24M04 Igkappa light chain (IGKC, IGK Human)

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

EEK promoter

Available since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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TL1 alphaDdCBE 3_NC (right, DddA11-C)

Plasmid

#222005

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alpha-3_NC right)-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

TALE: Q231R, W232G, S233A | DddA: T1413I

Promoter

CMV

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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TL1 alphaDdCBE 3_CN (right, DddA11-N)

Plasmid

#222003

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alpha-3_CN right)-TALE CTD-DddA11 1397N-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

TALE: Q231R, W232G, S233A | DddA: S1330I, A1341V,…

Promoter

CMV

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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HK13A Clone 13A light chain

Plasmid

#192182

Purpose

Clone 13A light chain (HK13A)

Depositor

Sidi Chen

Article

Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

Insert

Clone 13A light chain (HK13A)

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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KA2896_pPB-TREtight-Tkmin-cHA-IRESH2BmKO2

Plasmid

#124175

Purpose

piggyBac-based Dox-inducible expression (very weak)

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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KA2895_pPB-TREtight-TATAmin-cHA-IRESH2BmKO2

Plasmid

#124174

Purpose

piggyBac-based Dox-inducible expression (weak)

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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ND4.2-Right DdCBE-G1397-C- T1413I-GFP

Plasmid

#179686

Purpose

Expression of base editor in mammalian cells and for cell sorting

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

SOD2 MTS_3xHA_ND4.2 Right TALE_2aa_DddA G1397-C (T1413I)_1xUGI_P2A_GFP_ATP5B 3'UTR

Use

Tags

Expression

Mammalian

Mutation

T1413I

Promoter

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pLX304 CMV FKBP-V5-sTurboID-high affinity (N)

Plasmid

#153004

Purpose

Expresses high-affinity split-TurboID N-terminal fragment in mammalian cells

Depositor

Alice Ting

Article

Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

Insert

sTurboID-high affinity (N) (G78)

Use

Lentiviral

Tags

FKBP and V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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pMEH373 dTomato 3x high affinity MS2 loops

Plasmid

#168221

Purpose

Mammalian expression of dTomato with 3 high affinity MS2 loops

Depositor

Joshua Leonard

Article

Hung et al J Extracell Vesicles. 2016 May 13;5:3102

Insert

dTomato

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-mCherry-P2A-NLSFlpN-nMagHigh

Plasmid

#180585

Purpose

PA-Flp

Depositor

Won Do Heo

Article

Jung et al Nat Commun. 2019 Jan 18;10(1):314. doi:

Insert

Split FlpO

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_4D5-High-CAR-mCherry_PGK_4D5-Low-SynNotch_Gal4VP64_RHL133

Plasmid

#164822

Purpose

Lentiviral vector for Gal4 inducible AntiHER2 4D5-High CAR-mCherry and constitutive expression of antiHER2 4D5-Low Gal4VP64 Synthetic Notch

Depositor

Wendell Lim

Article

Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

Inserts

AntiHER2 4D5-High Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
AntiHER2 4D5-Low Gal4VP64 synthetic Notch receptor

Use

Lentiviral

Tags

mCherry

Expression

Mutation

Promoter

miniCMV and pGK

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CMVd1-NLS-GAL4-iLight2-VP16

Plasmid

#219550

Purpose

NLS-Gal4(1-65)-iLight2-VP16 construc expression in mammalian cells. pAAV vector.

Depositor

Vladislav Verkhusha

Article

Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

Insert

NLS-Gal4(1-65)-iLight2-VP16

Use

AAV

Tags

Expression

Mammalian

Mutation

The (SAGG)x4 linker between GAL4(1-65) and the PC…

Promoter

CMVd1

Available since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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